GC-ECD를 이용한 유기염소계 및 Pyrethroid계 농약 동시 분석법

김우성,이선화,김재이,정지윤,이명자,박영채,이영자,정성욱,이봉헌,박흥재 한국환경과학회 2003 한국환경과학회지 Vol.12 No.4

Pesticides were extracted from samples with 70% acetone and methylene chloride in order, and then cleaned up via open-column chromatography apparatus packed with florisil, and finally analyzed simultaneously the organochlorine and pyrethroid pesticides using GC(ECD). An ultra-2 fused silica capillary column was used to separate and identify the products. The resolution between the last isomeric peak of cypermethrin(59.987min) and the first isomeric peak of flucythrinate(60.043min) was not satisfactory. The last isomeric peak of fenvalerate(62.344min) and the first isomeric peak of fluvalinate(62.397min) were overlapped. Recoveries of soybean sample for the most pesticides were 73.3% to 102.4%. Detection limits were between 0.004 and 0.063 ㎍/㎎ when this method was used.

소형펀치시험의 유한요소해석에 의한 Mn-Mo-Ni 저합금강의 파괴강도 평가

李載奉,金民喆,吳聳俊,朴宰鶴,李奉相 대한금속재료학회 2002 대한금속·재료학회지 Vol.40 No.12

The critical fracture stresses(σ^*_f (sp)) in various low alloy steels for reactor pressure vessel(RPV) have been evaluated by finite element analysis(FEA) of small punch test in the cleavage temperature region(-150 ∼ -196℃). In order to improve the accuracy of the FEA results on small punch tests, the uniaxial stress-strain properties beyond the necking were optimized by comparing the results of uniaxial tensile test and its finite element analysis. The loaddisplacement curves and distances from center to fracture surface(D_f) of FEA results are in good consistence with the experimental results. The σ^*_f(sp) from small punch test in various reactor pressure vessel(RPV) steels were found to have a linear relationship to the values from the precracked specimens(σ^*_f(PCVN)). This result indicates that small punch tests could be a useful method to evaluate the cleavage fracture behavior of the low alloy steels.

마이크로프로세서를 부가한 방사선감시 시스템에 관한 연구

이수용,이봉재 漢陽大學校 基礎科學硏究所 1988 基礎科學論文集 Vol.7 No.-

본 연구에서는 방사선검출기로 사용된 섬광계수관으로부터 생성된 출력펄스을 직점 처리하도록 마이크로 프로세서를 부가한 방사선감시 시스템을 설계 제작하였다. 펄스신호 처리회로를 통해서 나온 펄스는 마이크로프로세서 시스템에 의해 계수되고, 본 연구에서의 소프트웨어 프로그램에 의해서 실행된 논리 연산처리에 의해 방사선량율로 변환되었다. 시스템의 동작특성을 가장 함축성 있는 방법으로 시험하기 위하여 표준방사선선원으로서 세슘-137(3 Ci)를 사용하였다. 본 연구에서의 방사선감시 시스템은 애널로그회로의 것과 비교할 때 오차가 5% 이내에서 잘 실행됨을 나타내었고, 검출기가 20mR/h를 초과하는 방사선량율로 피폭될 경우에 있어서는 경보(alarm)를 결정하는 실제적인 사용에 적용할 수 있었다. In the present work, a microprocessor based radiation monitoring system in which direct process the out pulses produced from the scintillation counter used as a radiation detector was designed and constructed. The pulses out from the signal processing circuit was counted by means of the microprocessor system, and then converted into dose rate by the logical operation which was performed using the software program in the present work. In order to test the toperational behaviors of the system, cesium-137(3 Ci) source was used as a standard irradiation source. Radiation monitoring system in this work has shown good performance within the error of 5% when it was compared with the analogue circuit and has been put into practical use of the alarm determination in the case of the detector is exposed to the excessive radiation of which the dose rate is over 20mR/h.

사람唾液 Superoxide Dismutase의 精製 및 性狀

이봉재,이병래,전현우,양종대,이근배 朝鮮大學校 附設 醫學硏究所 1986 The Medical Journal of Chosun University Vol.11 No.1

The purification and some properties of superoxide dismutase(O_(2-)-O_(2-) oxidoreductase, ECI. 15.1.1) of human saliva have been studied. Human saliva was collected from medical students, male and female together with smoking male students. The activity of superoxide dismutase was measured by the method of McCord and Fridovich. The superoxide dismutase was purified by ammonium sulfate precifitation, DEAE-cellulose and sephadex C-100 column chromatography. The results are summerized as follows : 1. Human saliva contain measurable amounts of superoxide dismutase. 2. The activities of superoxide dismutase. were 0.72U/㎖ in male and 0.73U/㎖ in female saliva. The activity of this enzyme was 0.81U/㎖ in smoker. Accodingly, there was no recognizabIe differences in the enzyme activity between smoker and non-smoker. 4. The human salivary superoxide dismutase was purified approximately 90 folds, and the molecular weight of the purified enzyme was estimated to be approximately 32,000 by gel filtretion. 4. Purified salivary superoxide dismutase has contained 1.83 atoms of copper and 2.00 atoms of zinc per molecule and was strikingly similar to the superoxide dismutase previonsly isolated from other cukaryotes by other investigatury. 5. The ultraviolet absorption spectrum of purified salivan- superoxide dismutase was 268㎚ which was similar to the value previously seen with the cytosolic enzyme of eukaryotes.

환경 독성 Peptide의 인지질과의 상호 작용 특성 분석

이봉헌,박홍재 한국환경과학회 2003 한국환경과학회지 Vol.12 No.1

The interaction of mastoparan B, a cationic tetradecapeptide amide from the hornet Vespa basalis, with phosphilipid bilayers was with synthetic mastoparan B and its analogue with instead of hydrophobic 12th amino acid residue in mastoparan B. MP-B and its derivative, [12-Ala]MP-B were synthesized by the solid-phase peptide synthesis method. MP-B and analogue, [12-Ala]MP-B adopted an unordered structure in buffer solution. In the presence of neutral and acidic liposomes, the peptides took an α-helical structure. The two peptides interacted with neutral and acidic lipid bilayers. These results indicated that the hydrophobic α-helix of MP-B critically affected biological and helical content.

Ortho-Chlorobenzylidene Malononitrile(CS) 이 흰쥐 피부조직에 미치는 조직학적 영향에 관한 연구

이우용,최봉근,김잉곤,엄기일,정호삼,정호삼,류재만 한양대학교 의과대학 1998 한양의대 학술지 Vol.18 No.1

This study was undertaken to describe the fine structural changes of cytoplasmic organelles in epidermal cells, and the changes of volume and localization of collagen type Ⅰ in the dermis of rat skin by CS(Ortho-chlorobenzylidene malononitrile) application. The experimental animals, 100∼150gm of body weight rats(Sprague-Dawley strain), were sacrificed at 12 hours, 24 hours, 3 day and 5 day after CS application on the hair-shaved back skin. The specimens were prepared for ultrastructural findings, ultrathin sections were stained with uranyl acetate and lead citrate double stain and observed with Hitachi-600 electron microscrope. The others were incubated with rabbit anti rat type Ⅰ collagen antibody as primary antibody, and Goat anti rabbit IgG antibodies as secondary antibody And advidin-biotin-conjugated(ABC) peroxidase procedure was used. All of specimens for immune reactions were observed with light microscope. The results obtained were as follows. 1 Keratinocytes appeared fully necrotized after 3 days of CS application. The epidermis showed degeneration of basal lamina, enlargement of intercellular spaces and necrotic keratinocytes with vacuole and vesicles. And large keratohyalin granules were seen in the shrunken epidermal cells. 2 At 3 or 5 day after CS treatment, the normal cytoplasmic organelles of keratinocytes began to appear on the basal and prickel cells. 3 At 12 hour an 24 hour after CS treatment. The immune reaction of type Ⅰ collagen showed weak reaction in reticular layer of dermis. But at 3 day and 5 day after CS treatment, moderate immune reaction were seen in throughout the dermis. These results suggest that CS application to rak skin may induce changes of keratinocytes and reductive changes of type Ⅰ collagen in the dermis.

조직배양에서 얻어진 쪽파의 유전적 변이체들이 보여주는 단백질과 Isozyme의 유형분석

朴敬淑,李在悅,徐奉甫 慶北大學校 1992 論文集 Vol.52 No.-

Some genetic variants of Amphihaploid, Hypoamphidiploid, Amphidiploid are regenerated from tissue culture in Allium wakegi. They were applied to investigate the effects of the chromosomal differences on the gene expression of some isozymes. The electrophoretic migration patterns of total proteins were different at the position of MW5×10^5 Dalton among the genetic variants. Three different isozymes were characterized from the migration patterns of isozymes in the discontinuous polyacrylamide gel electrophoresis. Alcohol dehydrogenase(ADH) appeared 3 bands in the gel electrophoresis corresponding to 3 isozymes, that is allozymes distributed as single isozyme in each individual. Isocitric acid dehydrogenase(ICDH) appeared 5 bands in the gel and corresponds to 5 isozymes as allozymes which distributed single isozyme in each individual. Phosphoglucoisomerase(PGI) appeared 8 bands in electrophoresis and to 8 isozymes which originated from 2 genes and distributed more than two isozumes in each individual. According to the isozume expression in the genetic variants of Allium wagegi, the amplification of chromosomal numbers can affect neither to increase the isozyme numbers and concentration nor to express heterogeneous types in the genetic variants.

Ⅲ급 부정교합자의 연조직 측모 감별에 관한 연구

황병남,이승훈,이정근,이재봉 대한악안면성형재건외과학회 2000 Maxillofacial Plastic Reconstructive Surgery Vol.22 No.2

This study was performed to investigate the characteristics of soft tissue profile of the class III malocclusion and to test the yardstick far differential diagnosis between surgical and orthodontic patients Initial lateral cephalograms of orthodontic group(30 patients) that have acceptable occlusion and profile by orthodontic treatment alone and surgical group(30 patients) that have favorable occlusion and profile by combined surgical-orthodontic treatment were selected in Ajou university hospital. Powell and Burstone II analysis were made on the tracing. Descriptive, comparative, factor, cluster, and discriminant analysis were carried out with computer program. The results were as followings : 1.Patients who received surgery had a more concave profile and a longer lower facial height than patients who received orthodontic treatment alone. 2.Nasolabial angle, ratio of vertical height, and mentolabial sulcus were significantly different at the 5% level. And facial protuberance, upper lip protuberance, mentocervical angle, nasofrontal angle, nasomental angle, mandibular vertical height, angle between cervix and lower face, ratio of mandibular vertical height divided by cervical depth, ratio of vertical height between upper and lower lip, and maxillary protuberance were significantly different at the 1% level. 3.8 factors were extracted and factor 2, 3, and 8 showed significant differences by factor analysis. 4.Orthodontic group (25) and surgical group (35) were classified by cluster analysis. 5.Discriminant function was D = 0.079Nasomental angle + 0.081Sn-Gn + 3.343Sn-Gn/C-Gn + 1.734Sn-St/St-Me'-26.460, and cutting score was 0, so we can discriminate that orthodontic group has the score above 0, and surgery group below 0. And 91.7% of original grouped cases were correctly classified.

하청 근로자들의 건강수준 평가

최홍열,고상백,장세진,차봉석,임형준,이상윤,김재용,강동묵,조수헌 大韓産業醫學會 2001 대한직업환경의학회지 Vol.13 No.1

목 적 : 하청노동을 이용한 노동 유연화 전략은 근로자들의 고용을 불안정시키고, 근로자들의 삶에 부정적 영향을 미치고 있다. 따라서 이 연구는 하청업체 근로자들의 고용현황을 조사하고, 기존의 업체중심의 건강평가의 문제점을 지적하고자 하였다. 또한 하청업체 근로자들의 건강평가의 현재적 의미와 사회적 배제의 특성을 알아보고자 하였다. 방 법 : 모기업과 하청업체 근로자들의 건강수준을 비교하기 위하여, 정기건강진단(일반, 특수)과 채용건강진단 자료를 이용하고자 하였다. 건강진단에서 누락된 비정규직을 포괄하기 위하여 SF-36을 이용하여 건강설문조사를 시행하였다.결 과 : 조선업 하청업체 근로자들의 고용기간은 짧고, 빈번한 노동이동 현상을 보였다. 3년간 건강진단 수검 현황을 통해 살펴볼 때, 1998년 수검자중 2000년까지 연속해서 검진 받은 수검자는 40.3%였다. 건강진단 결과 유소견자 규모를 통해 모기업과 하청업체간의 건강수준을 비교해 보았다. 모기업은 일반질병 요관찰자와 질병자를 포함한 유소견자가 1,011명으로 약 10 %의 유소견율을 보이고 있고, 질병자의 경우 596명으로 유병률이 5 %를 상회하고 있다. 반면 하청업체의 경우 1,967명의 수검자중 유소견자가 115명으로 6 %의 유소견율을 보이고있고, 질병자는 82명으로 4 %의 유병률을 보이고있다 채용건강진단을 받은 수검자는 6,260명 이었으며, 이 중 유소견자 수는 2,373명으로 전체 수검자의 37.9 %를 차지하였다. 건강설문지의 경우는 전체 건강수준의 총합은 하청보다 원청 근로자가 높았고 통계적으로 유의하였다. 결 론' 정기건강검진 결과는 하청근로자들이 고용형태에 따라 검진을 받지 못한 경우가 많아, 건강 근로자 효과로 표현될 가능성이 있다. 따라서 채용건강진단 결과는 다른 측면에서 하청업체 근로자들의 건강수준의 현실적 반영이라고 할 수 있다. 향후 하청업체 근로자들의 건강문제를 감시하고 개선하려면 기존의 업체 중심의 접근으로는 하청 근로자의 건강문제를 제대로 다룰 수 없고, 업종과 지역을 동시에 고려하여 접근할 필요가 있다. 또한 단기 고용 근로자 및 비정규직 근로자를 사회보장의 확충과 모기업의 연대책임 강화 및 기업복지 차원에서 포괄하는 방안이 모색되어야 하겠다. Objectives : The strategy for the labor flexibility through subcontracted labor have brought a negative effect on the lives of the workers, such as the increase of the unstable employment. This study was conducted to analyze the relationship between the employment of subcontracted firms and the health status of them, and to find out some problems which have been caused in the process of health evaluation and the characteristics of social exclusion. Methods : We reviewed the periodical health examination and preemployment health examination in order to compare health status of the subcontracted workers with those of the parent firm workers. The SF-36 questionnaire was administered to assess the self-perceived health status. Results : The result shows that compared to the working duration of the parent firm workers, those of the subcontracted workers were shorter, and the job transfer rate was higher. In the periodical health examination, the prevalence of occupational and general disease was higher in the parent firm(10%) than in the subcontracted firms(6%). Preemployment health examination plays an important role in worker's health manage- ment. Among 6,260 workers who received preemployment health examination, the prevalence of disease was 37.9%. Mean scores of health status measured by SF-36 were lower in the subcontracted firm than in the parent firm. Conclusions : Due to social exclusion of the subcontracted workers from the periodical health examination, there is a possibility that the periodical health examination have the healthy worker effect. These results suggests that the preemployment health examination could be a more appropriate indicator which is able to assess the health status of the subcontracted workers than the periodical health examination.

우리나라 行政區劃線의 不合理要因과 그 改善方向에 관한 硏究

金裕赫,琴東信,梁在乾,李鳳三 단국대학교 법정대학 지역개발학과 1980 地域開發論叢 Vol.2 No.-

1. This thesis aims at analyzing the characteristics of the demarcation line of the administration areas in Korea by reviewing the history and background of how it was drawn and at the same time at exploring ways of correcting factors contributing to problems and ineffectiveness of administration. In order to collect data of opinions on this matter from officials actually assigned to the counties and their sub-level administration areas where administration is bifurcated in two separated blocks of areas, irrespective of the administrative efficiency and convenience of the populace in the areas, we selected eight kuns of this nature and sent questionaires to 488 chiefs and leading officials of kuns, myuns and villages. A total of 237 persons, 48.6% out of 488 responded. This data proved to be very expedient and instrumental in carrying out this study. 2. One of the characteristics of the demarcation line of administration areas is that it is drawn on the basis of natural conditions such as the extention of mountains, meandering lines of rivers, the formation of the community, traditional reasons, and so forth rather than on the theoretical as well practical backgrounds imbued with administrative philosophy, which seeks for rational and balanced development of a whole community with two factors in mind: the principle of spatial division as an administrative skill and the principle of spatial harmony for balanced division of administration zones. However, it is true that a large number of local inhabitants have felt inconveniences due to the boundary lines being zigzag or meandering. When we surveyed on the frequency of going to market as an indicator of economic activities of the people living along the boundary lines, it is found that almost 77.6% of the people questioned answered that they were going to markets located in the neighboring kun rather than those in their own administration zones. The factors that lead to choosing markets are usually two: one is spatial distance and the other is the peoples norm of life. Although the nature of the responses may vary according to where people live within the divided block of the administration area, a total of 86.6% of the questioned expressed their hope that the boundary lines would be straightened. This reflects their wishes of improving the efficiency of local administration and convenience of living for effectiveness of the administration cantrol. For example, state boundary lines of the U.S and Australia belong to the theoretical type is whereas sung boundary lines of China and kun(county) demarcation lines of Korea belong to the natural condition (or setting) type. Especially, some of administration demarcation lines of Korea have been zigzay so that one kun is divided into two or more separate blocks of areas as the result of city administration areas within the kun becoming enlarged. There are eight cases of this nature in Korea. 3. Our survey which was conducted to discover in part the extent of awareness of such bifurcation or division by the inhabitants living in the divided zones, showed that only 83.4% of those who responded said that they had knows the fact and the remaining 15.5% were quite unaware of the fact until they came to realize it upon reading our questionaire. This extent of unawareness means that local ranking administration leaders did not pay much attention to the effect of the local administration with respect to the boundary lines. 4. Speaking from the above finding, we are to propose that irrational factors related to the present administration demareation lines be corrected and improved for the following reasons: 1) To enhence the efficiency of administration, 2) To reestablish the system of utilization of resources 3) And finally to enlarge the integrated potentials of various social dynamics. We hope that efforts will be made to straighten administration demarcation lines as far as possible, taking into consideration the opinions and wishes of the people living in the affected areas and zones so that directions of development and improvement on this matter may be effectively explored. At the same time we propose the establishment of long range policies with a view of eliminating disparity in the development between the regions, by implementing necessary measures for development of local cities.