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강준구,Sungjin Ko,W. Barney Smith,Heung-Chul Kim,이인용,Joon-Seok Chae 대한수의학회 2016 Journal of Veterinary Science Vol.17 No.2
North Korea is located on the northern part of the Korean Peninsula in East Asia. While tick-borne pathogens of medical and veterinary importance have been reported from China and South Korea, they have not been reported from North Korea. To screen for zoonotic tick-borne pathogens in North Korea, ticks were collected from domestic goats. A total of 292 (27 nymph, 26 male, 239 female) Haemaphysalis (H.) longicornis were collected and assayed individually for selected tick-borne pathogens. A total of 77 (26.4%) were positive for Anaplasma bovis, followed by Bartonella (B.) grahamii (15, 5.1%), Anaplasma phagocytophilum (12, 4.1%), Bartonella henselae (10, 3.4%), and Borrelia spp. (3, 1.0%) based on 16S ribosomal RNA and ITS species-specific nested polymerase chain reaction. Using the groEL-based nested PCR, a total of 6 and 1 H. longicornis were positive for B. grahamii and B. henselae, respectively. All products were sequenced and demonstrated 100% identity and homology with previously reported sequences from other countries in GenBank. This is the first report of the detection of tick-borne pathogens in the North Korea and suggests that farm animals may act as reservoirs for zoonotic tick-borne pathogens.
Comparative proteomic analysis of outer membrane protein 43 (omp43)-deficient Bartonella henselae
강준구,이희우,Sungjin Ko,Joon-Seok Chae 대한수의학회 2018 Journal of Veterinary Science Vol.19 No.1
Outer membrane proteins (OMPs) of Gram-negative bacteria constitute the first line of defense protecting cells against environmental stresses including chemical, biophysical, and biological attacks. Although the 43-kDa OMP (OMP43) is major porin protein among Bartonella henselae-derived OMPs, its function remains unreported. In this study, OMP43-deficient mutant B. henselae (Δomp43) was generated to investigate OMP43 function. Interestingly, Δomp43 exhibited weaker proliferative ability than that of wild-type (WT) B. henselae. To study the differences in proteomic expression between WT and Δomp43, two-dimensional gel electrophoresis-based proteomic analysis was performed. Based on Clusters of Orthologus Groups functional assignments, 12 proteins were associated with metabolism, 7 proteins associated with information storage and processing, and 3 proteins associated with cellular processing and signaling. By semi-quantitative reverse transcriptase polymerase chain reaction, increases in tldD, efp, ntrX, pdhA, purB, and ATPA mRNA expression and decreases in Rho and yfeA mRNA expression were confirmed in Δomp43. In conclusion, this is the first report showing that a loss of OMP43 expression in B. henselae leads to retarded proliferation. Furthermore, our proteomic data provide useful information for the further investigation of mechanisms related to the growth of B. henselae.
(DNA-CTMA/형광염료)복합계를 이용한 측면발광 증폭 연구
강준구,박영민,파하드마틴,홍성규 한국공업화학회 2019 한국공업화학회 연구논문 초록집 Vol.2019 No.1
DNA-CTMA 복합체는 낮은 광학 손실, 높은 열 안정성뿐만 아니라 빛의 방출을 증폭시킨다고 알려져 있다. 이러한 DNA-CTMA 복합체에 형광염료 물질을 혼합하게 되면 염료 분자는 DNA와 직접적으로 반응하지 않고, DNA의 이중나선 구조와의 분자 간 상호작용을 통해서 발광 효율의 증대가 기대된다. 본 연구에서는 DNA-CTMA 복합체와 형광 염료를 포함한 혼합염료를 측면발광 소자에 활용하여 발광 증폭을 확인하고자 한다.
Phenobarbital 前處置가 Halothane 麻醉時 肝病變에 미치는 影響에 關한 病理組織學的 硏究
姜俊求,姜大榮 충남대학교 의과대학 지역사회의학연구소 1984 충남의대잡지 Vol.11 No.2
Since halothane, as a new potent, nonexplosive, volatile agent, was introduced in 1956, many case reports of liver necrosis following halothane anesthesia have raised to possibility that the agent may damage the liver under certain circumstances. This study was carried out to investigate the histopathological effects of phenobarbital pretreated halothane anesthesia to the rat liver cells. Sprague-Dawley rats (male and female) were pretreated with phenobarbital; PBT(100 mg/kg/ day) for 3 days. All animals in each experimental group (saline-control, saline-anesthetized, PBT-control, and PBT-anesthetized) were fasted for 15 to 16 hours prior to each anesthesia period. PBT and Saline-pretreated animals were anesthetized together for 3 hours one to two times at 3 day intervals in a single chamber from a calibrated vaporizer. Stage III anesthesia was introduced with 1% halothane in oxygen and maintained with 0.8% to 1% halothane. Controls received the equivalent amount of saline solution. Each animal was sacrificed immediately, at 6, 12, 18, 24 and 48 hours after anesthesia respectively. The histopathological changes in the liver of the control and experimental groups were noted as follows; 1. Vacuolar degeneration of the hepatic cells of PBT pretreated rats showed more marked degree than the control. In repeated exposure of animals to the anesthetic at 3 day intervals aggravates the lesioned in control and PBT pretreated rats. 2. In first exposure to the anesthetic, fatty changes of the hepatic cells in PBT pretrea rats showed more marked in degree than the control. In repeated exposure of rats aggravates the lesions in control and PBT pretreated rats, and that of PBT pretreated rats was similar to the control. 3. The marked necrotic changes of hepatic cells were present in the PBT pretreated rats but those of the control were rarely observed. Repeated exposure of rats aggravate the lesion.
산업연관분석에 의한 한중일 물류 서비스의 구조와 파급효과 분석
강준구,이홍식,정승연 서울대학교 국제학연구소 2011 국제지역연구 Vol.20 No.4
This paper aims to analyze the current situation of Korean logistics industry. To conduct an inter-industry analysis, we attempt an international comparative analysis for logistics service of the three nations, South Korea, China, and Japan. This study uses IO tables of each country published by the OECD and Asian International IO table provided by IDE․JETRO(2006). The three countries were compared in terms of labor productivity, spillover effect on value-added, employment of logistics service, and productivity of logistics service among each other. The results indicate that Japan yields relatively high value-added in logistics service with less labor input while China still depends largely on labor input, in spite of recent improvements in productivity. In the Korean logistics industry, employment accounts for a much greater part of the national economy than output and value-added do. This implies that the Korean logistics service is a low value-added industry compared to that of China and Japan, as well as other Korean industries. This paper concludes that without first improving its low productivity, it will be difficult for Korea to develop a domestic logistics service and enhance its competitiveness. 본 논문의 목적은 한국 물류서비스 산업의 현 위치를 객관적인 입장에서 정확히 파악하고자 함이다. 이를 위해 산업연관분석의 응용분석 방법을 이용하여 OECD의 OECD.StatExtracts에서 발표하고 있는 각국의 산업연관표 자료와 고용 통계, 그리고 IDE․JETRO(2006)에서 제공하고 있는 Asian International Input-Output Table 등의 자료를 사용함으로써 한중일 3국의 물류서비스에 대한 국제 비교분석을 시도하였다. 3국의 비교를 위하여 각국의 물류서비스에 대한 노동생산성은 물론 직간접적인 부가가치 파급효과와 물류서비스의 고용에 대한 파급효과를 동시에 고려하여 한중일 3국의 물류서비스 생산성을 비교 분석하였다. 분석 결과에 따르면, 한중일 3국 중 일본은 물류서비스 분야에서 노동투입은 상대적으로 적고 부가가치를 많이 창출하는 양상을 보인 반면, 중국은 2000~2005년 동안 다소간의 개선 양상을 보였음에도 불구하고 여전히 노동투입에 많이 의존하는 특징을 나타내었다. 이에 반해 한국의 경우 물류서비스가 국민경제에서 차지하는 총산출 및 부가가치 비중보다 고용 비중이 월등히 높은 것으로 분석되었다. 이와 같은 결과는 한국의 물류서비스 분야가 국내 여타산업뿐만 아니라 중국과 일본의 물류서비스 분야와 비교해 볼 때 상대적으로 저부가가치 산업이라는 사실을 보여주는 것으로 이러한 문제점의 개선 없이는 국내 물류서비스의 발전과 경쟁력 제고는 상당히 어려울 것이라는 점을 말해 주고 있다.