한국인 다기관 암 코호트 시료의 DNA 생활성도 평가

양미희,유지현,김청식,신애선,강대희,장성훈,박수경,신해림,유근영,Yang, Mi-Hi,Yoo, Ji-Hyun,Kim, Cheong-Sik,Shin, Ae-Sun,Kang, Dae-Hee,Chang, Soung-Hoon,Park, Sue-Kyung,Shin, Hai-Rim,Yoo, Keun-Young 대한예방의학회 2003 예방의학회지 Vol.36 No.4

Objectives : Peripheral blood-buffy coat fractions (N=14,956) have been stored at $-70^{\circ}C$ in the headquarter of the Korean Multicenter Cancer Cohort (KMCC), since 1993. To study the future molecular etiology of cancers using specimens of the cohort, properly stored specimens are necessary, Therefore, the DNA-viability of the bully coat samples was investigated. Methods : Buffy coat fraction samples were randomly selected from various collection areas and years (N=100). The DNA viability was evaluate from the UV-absorbent ratios at 260/280nm and the PCH for $\beta$-globin was performed with genomic DNA isolated from the buffy coat. Results : PCR products were obtained from 85 and 98% of the C and H area-samples, respectively, using 50 or $100{\mu}l$ of the buffy coat. There were significant differences in the yields of the PCR-amplifications from the C and H areas (p<0.05), which was due to differences in the homogenization of the buffy coat fractions available as aliquots. The PCR-products were obtained from all of the samples (N=7) stored at the C area-local confer, but the other aliquots stored at the headquarter were not PCR-amplified, Therefore, the PCR products in almost all the samples, even including the DNA-degraded samples, were obtained. In addition, an improvement in the DNA isolation, i,e. approx. 1.6 fold, was found after using extra RBC lysis buffer. Conclusions : PCR products for $\beta$-globin were obtained from nearly all of the samples. The regional differences in the PCR amplifications were thought to have originated from the different sample-preparation and homogenization performance. Therefore, the long term-stored buffy coat species at the KMCC can be used for future molecular studies.

[서평 (3)] 성숙한 신앙으로 삶을 살아 내기 위한 한 이성적 접근

양미희 대한기독교서회 2015 基督敎 思想 Vol.676 No.-

내부표준물질, 9-hydroxyphenanthrene을 이용한 뇨 중 1-hydroxypyrene의 새로운 정량법

양미희,이호선,신민경,김용학 숙명여자대학교 약학연구소 2005 약학논문집-숙명여자대학교 Vol.22 No.-

Urinary 1-hydroxypyrene (1-OHP) has been used as exposure biomarkers for polycyclic aromatic hydrocarbons and analyzed with HPLC/FD after preparation by column extraction or simple centrifuge. However, the above methods have several drawbacks such as high noise or large volume of urine. In this study, we developed a new analysis method with liquid-liquid extraction and purification of commercial enzymes. We analyzed uirnary 1-OHP in 27 spot urines ofa subject in 3 weeks with the new method. As result, we could analyze all 27 samples clearly. The resolution of the new method was better than the previous centrifuge method. Therefore, the new method is a recommendable analysis method for urinary 1-OHP. Considering both of easiness and accuracy of the experiments, the new method should be further compared with the previous methods.

Development and application of biomarkers for monitoring of environmental toxicants in humans

양미희 환경독성보건학회 2006 한국독성학회 심포지움 및 학술발표회 Vol.- No.-

대학생의 결혼관에 대한 연구

양미희,권혜성 명지대학교 1986 家政學硏究 Vol.2 No.-

한국인에서 DPD 및 TS, MTHFR의 유전자다형

양미희 숙명여자대학교 약학연구소 2004 약학논문집-숙명여자대학교 Vol.21 No.-

In-order to do efficient treatment of 5-Fluorouracil (5-FU) in Koreans, we investigated genetic polymorphisms of dihydropyrimidine dehydrogenase (DPD; gene, DPYD), thymidylate synthase (75), and methyltetrahydrofolate reductase (MTHFR), which are involved in 5- FU metabolism or efficacy in a Korean population. As results, we found genetic polymorphisms in DPYD-85, -1627, and -1896 site, TS-5'ER, and -3'U'TR, and MTHFR-222, 429 site among the Koreans (normal, N=105; head and neck cancer patients, N=28). 5-FU efficacy was determined into PR(partial response, decrease of cancer size over 50%) and SD (stable disease). Each or combination of the above 7 genetic polymorphisms were not associated with 5-FU efficacy. Therefore, the studied 7 genotypes can not be considered to result in individual variation in 5-FU efficacy.

한국산 배(梨)의 항돌연변이 및 항암효과

양미희,박장환,김대중,정헌상 대한암예방학회 2005 Journal of cancer prevention Vol.10 No.2

We already reported that consumption of Korean pears had inverse association with level of urinary 1-hydroxypyrene (1-OHP), which is an exposure biomarker and metabolite of polycyclic aromatic hydrocarbons (PAHs). As PAHs include various carcinogens such as benzo(a)pyrene, our report showed the chemopreventive potential of Korean pears. Recently, we studied effects of Korean-pears on PAH-induced mutagenicity and carcinogenicity, i.e. antimutagenic effects with MN (micronuclei) test and Comet assay, and anticarcinogenic effects with animals. Finally, we performed an intervention study with the pears in volunteers using toxicokinetic methods. As results, we found that Korean pears have antimutagenic, antioxidative, and anticarcinogenic effects against PAHs exposure and rapid excretion of parental PAHs. Therefore, the intake of pears after PAHs exposure such as BBQ is thought as an ideal lifestyle to prevent PAH-induced cancers. (Cancer Prev Res 10, 124-127, 2005)

Individual Variations in Toxicity of Tobacco Smoking

양미희 대한암예방학회 2005 Journal of cancer prevention Vol.10 No.3

Tobacco smoking is a well-known cause of cancers and has been emphasized not only to clarify the mechanism of carcinogenesis but also to prevent the smoking-related cancers. In addition, the Korean female passive smokers showed early onset of lung cancer and high incidence of adenocarcinoma compared to males. Therefore, several factors are suspected as etiology of female passive smokers, e.g. ETS (environmental tobacco smoking), and high sensitivity to carcinogens via genetic and hormonal factors. Traditional epidemiological data showed controversial results of sex or gender differences in lung cancer susceptibility. Therefore, many confounders were expected in those studies. Among them, one is due to self reported smoking, therefore, the effects of ETS were not thoughtfully considered. The consequence of this is that there may be an overestimation of baseline lung cancer incidence or underestimation of the ORs in female smoke. To obtain undistorted mechanisms of etiology in side stream females, proper exposure monitoring of ETS and considering of susceptibility markers are recommended. For the exposure monitoring, urinary cotinine or naphthols are recommendable, while individual variation in their metabolisms, such as genetic polymorphisms in CYP2E1 and 2A6 and GSTT1 and GSTM1 among Asians should be understood. For susceptibility factors of lung cancer in passive smoker females, genetic polymorphisms of the CYP1A1 exon 7 Ile462Val and the GSTP1 exon 5 Ile105 Val are recommended. Sensitive to tobacco carcinogenic PAHs or CYP1A1 and ER cross talk are possible mechanism of the susceptibility biomarkers in the passive smoker females. (Cancer Prevention Res 10, 135-148, 2005)

한국인의 고지혈증과 CETP 및 APOE 유전자다형

양미희,양지연,윤여정 숙명여자대학교 약학연구소 2006 약학논문집-숙명여자대학교 Vol.23 No.-

Since 1980s, hyperlipidemia in Korea has been rapidly increased and its related cardiovascular diseases are one of the main causes of death in Korea. Thus, for effective prevention of hyperlipidemia, clarification of etiology of the hyperlipidemia in Koreans is required. In this study, we focused on polymorphisms in genes, which are involved in cholesterol-transfer, and -binding, e.g. cholesteryl ester transfer protein (CETP) and apolipoprotein E (APOE). Study subjects were 257 Koreans (cases who had hyperlipidemia, LDL≥130, N=47; controls, N=210; Men, 62%). Genetic polymorphisms were determined by PCR-RFLP, and single base primer extension assays. As results, allele frequency of the CETP -Tag IB1 was significantly higher in cases than that in controls (p<0.05). When age and BMI were controled for statistics, there was 2.3 fold increase of OR (95%CI, 1.1-5.1) for hyperlipidemia in the Bl/Bl homozygotes compated to the B1/B2 heterozygotes. However, 2 SNPs, CI 12R and R158C of the APOE, or 5 haplotypes of the 2 SNPs were not associated with hyperlipidemia risk. Therefore, presence of the CETP-B1 allele has a potential of risk for Korean hyperlipidemia.

내분비장애물질노출이 유방암에 미치는 영향 -비스페놀 A를 중심으로

양미희 대한암예방학회 2006 Journal of cancer prevention Vol.11 No.4

Incidence ratio of breast cancer to total cancer has been increased in late 2 decades in Korea and breast cancer ranks second as a cause of death in women in recent Korea. In addition, exposure to endocrine disruptors has been emphasized as a new etiology of breast cancer. In this study, the effects of bisphenol A exposure on breast cancer in Korean women (100 breast cancer patients; 100 hospital controls) were investigated. Conjugated form of blood bisphenol A was designated as an exposure biomarker and analyzed with HPLC/FD. As results, blood bisphenol A could be detected in 94% of the subjects and the range of blood bisphenol A was 0.69~33.7μg/L (median, 5.3μg/L). The geomeric mean of bisphenol A was somewhat higher in the cases than that in the controls, however, was not statistically significant due to multivariate analysis including age, alcohol dringking, breast feeding, and age of menarche. Thus, bisphenol A exposure may not work as a breast cancer risk factor. (Cancer Prev Res 11, 273-277, 2006)