http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Erythromycin 내성 A군 연쇄구균 출현과 관련된 세포벽 T 단백 항원성 검사의 역학적 중요성
차성호,황규잠,이영희 대한소아청소년과학회 2000 Clinical and Experimental Pediatrics (CEP) Vol.43 No.10
목 적 : A군 연쇄구균의 혈청학적 특이성(specificity)을 확인하는 방법으로 혈청형 분류(serotyping)와 항생제 감수성검사를 시행하여 같은 시기에 서로 다른 지역에서 분리된 연쇄구균의 혈청형 분류로 지역별로 균의 동태를 감시하고, 질병의 발생이나 내성균의 출현 등 역학적 변화가 있을 때 이용할 수 있는 역학적 검사 방법으로서의 유용성을 판단하고자 하였다. 방 법 : 1996년부터 1998년 사이에 정상 초등학교 소아 1,294명을 대상으로 경부배양검사를 시행하여 보균자로부터 얻어진 A군 연쇄구균과 1998년 A군 연쇄구균 감염 환자로부터 얻어진 92개 균주를 대상으로 역학적으로 유용한 T 단백혈청학형 분류와 항생제 감수성 검사 그리고 최소억제 농도를 측정하였다.결 과 : 울진으로 부터 분리된 균주의 T 단백형 분류에서 EM 내성 T 혈청형과 관련 있는 T12은 1996년 4.2%, 1997년 14.3%, 1998년 45.7%로 증가하는 양상을 보였고, 오산에서 1996년에 분리된 132개 균주 중 T12은 7.6%, T28 은 3.8%, T4는 4.5%, T1은 6.0%로 1996년도의 EM 내성 T 혈청형의 빈도는 적었다. 1998년 환자로부터 분리된 균주의 T 단백형 분리 분포는 T12가 41주(44.7%), T4가 18주(19.6%), T28은 4주(4.3%), T1은 4주(4.3%)였다. EM에 대한 내성은 92주 중 38주(41.3%)가 내성균이며 T12로 분리된 41주 중 EM, clindamycin, tetracycline에 교차 내성을 나타내는 균주는 27주(65.8%)였다. Purpose : The identification of antigenic specificity of Streptococcus pyogenes using T serotyping is important to understand biologic characteristics of microorganisms. We would like to disover the association of the occurrence of predominant T type, with possible outbreak of erythromycin resistant Streptococcus pyogenes in this country, which has been documented since the late 1990s. Methods : Throat swab cultures were taken from a total of 1,294 normal school children(Subject A) in two different geographical areas. A total of 92 strains(Subject B) were obtained from the patients with group A streptococcal infections from Jan. 1998 to Dec. 1998. All strains were serotyped with T protein antisera. Results : The distribution of T12 in Uljin increased from 4.2%(1996) to 45.7%(1998). T4 increased from 6.3% to 20.0%. Thirty-eight out of 92 strains were resistant to erythromycin. Twenty-seven out of 41 strains(T12) were multidrug resistant to erythromycin, clindamycin, and tetracycline. Conclusion : We can see the sudden increase in T12 strains, one of the strains that are resistant to erythromycin in 1998, compared with previous years. T protein serotyping could be epidemiologically useful as a screening methods for detecting erythromycin resistant group A streptococci in hospitals where the routine antibiotic sensitivity test dose not examin for streptococci.
Fluoroquinolone계 항균제의 penicillin 내성 폐렴구균(Streptococcus pneumoniae)에 대한 항균력 비교
이광준,배송미,황규잠,이영희,김기상 대한화학요법학회 2002 대한화학요법학회지 Vol.20 No.3
목적 : 최근들어 penicillin 내성 폐렴구균의 급속한 증가와 전세계로의 확산은 심각한 문제를 야기하고 있다. 1990년대 들어 그람음성 세균뿐 아니라 그람 양성 세균에 대한 항균력이 향상된 많은 fluoroquinolones계 항균제의 개발에 힘입어 폐렴구균성 감염질환 치료시 사용이 증대되고 있으며 한편, 이와 더불어 이미 fluoroquinolone계 항균제에 대한 내성을 보이는 폐렴구균에 대한 보고가 증가하고 있는 실정이다. 이에 본 실험에서는 국내 임상 분리 폐렴구균 중 penicillin에 고도내성을 보이는 균주를 대상으로 하여 fluoroquinolones계 항균제에 대한 감수성 여부를 확인하고자 하였다. 방법 : 항균제 감수성 검사는 3% 말혈액이 첨가된 Mueller-Hinton broth를 사용한 액체배지 미량희석법을 이용하여 NCCLS에서 제시한 방법에 준하여 실시하였다. 결과 : MIC_90를 기준으로 하였을 때 본 실험에서 사용한 5가지의 fluoroquinolones계 한균제 중 gatifloxacin과 moxifloxacin(MIC_90, 0.5㎍/㎖)이 ciprofloxacin (MIC_90, 4㎍/㎖), levofloxacin (mic_90, 2㎍/㎖) sparfloxacin (MIC_90, 1㎍/㎖)에 비해 penicillin 내성 폐렴구균에 대한 항균력이 높게 나타났다. 결론 : 5가지의 fluoroquinolones계 항생제 중 최근들어 새로이 개발된 항생제인 moxifloxacin과 gatifloxacin이 ciprofloxacin, levofloxacin, sparfloxacin보다 폐렴구균에 대한 더 우수한 항균력을 가지고 있음을 확인하였다. 한편, 국내 페니실링 고도 내성 폐렴구균에서 fluoroquinolones에 대한 고도 내성을 보이는 균주의 출현은 fluoroquinolones 내성 균주의 급속한 증가와 확산에 대한 지속적인 감시와 대책 마련이 요구된다. Background : Recently the rapid increase and global spread of penicillin-resistant S. pneumoniae has become a serious problem. In the 1990s, a varisty of novel fluoroquinolones with enhanced activity against gram-positive bacteria have been developed and used for the treatment of pneumococcal infections. In such situations, the fluoroquinolone-resistance in S. pneumonaie has been reported from many countries. In this study, we compared the activity of ciprofloxacin, levofloxacin, sparfloxacin, gatifloxacin and moxifloxacin against 93 isolates of S. pneumoniae which were highly- resistant to penicillin (MIC 2~8㎍/㎖). methods : Antimicrobial susceptibilities were determined by the broth microdilution method in cation-adjusted Muller-Hinton broth supplemented with 3% lysed horse blood according to the guidelines of the National Committee for Laboratory Standards (NCCLS). Results : Based on MIC_90s, sparfloxacin and moxifloxacin were the most potent fluoroquinolone tested against penicillin-resistant S. pneumoniae and retained excellent activity comparable with ciprofloxacin and levofloxacin. The rank order of bactericidal activity was ciprofloxacin (least active)《 levofloxacin 〈 sparfloxacin 〈 gatifloxacin and moxifloxacin (most active). Conclusion : In this study, the newer fluoroquinolones (moxifloxacin, sparfloxacin)showed improved activity over that observed with ciprofloxacin, levofloxacin, and sparfloxacin. The emergence of highly resistance to fluoroquinolones among penicillin-resistant pneumococci in Korea call for continuous attention and strategies to prevent rapid increase and spread of these strains.
Seroreactivity to Q Fever Among Slaughterhouse Workers in South Korea
주혁,유석주,황규잠,임현술,이관,박미연 대한예방의학회 2017 Journal of Preventive Medicine and Public Health Vol.50 No.3
Objectives: Q fever is a zoonotic disease that occurs worldwide; however, little is known about its prevalence in South Korea. We attempted to determine the prevalence of Q fever seroreactivity among Korean slaughterhouse workers and the risk factors for seroreactivity according to the type of work. Methods: The study was conducted among 1503 workers at a total of 73 slaughterhouses and 62 residual-product disposal plants. During the study period, sites were visited and surveys were administered to employees involved in slaughterhouse work, and serological tests were performed on blood samples by indirect immunofluorescence assays. Serological samples were grouped by job classification into those of slaughter workers, residual-product handlers, inspectors and inspection assistants, and grading testers and testing assistants. Employee risk factors were analyzed according to the type of work. Results: Out of 1481 study subjects who provided a blood sample, 151 (10.2%) showed reactive antibodies. When these results were analyzed in accordance with the type of work, the result of slaughter workers (11.3%) was similar to the result of residual-product handlers (11.4%), and the result of inspectors and assistants (5.3%) was similar to the result of grading testers and assistants (5.4%). Among those who answered in the affirmative to the survey question, “Has there been frequent contact between cattle blood and your mouth while working?” the proportions were 13.4 and 4.6%, respectively, and this was identified as a risk factor that significantly varied between job categories among slaughterhouse workers. Conclusions: This study found a Q fever seroreactivity rate of 10.2% for slaughterhouse workers, who are known to be a high-risk population. Contact with cattle blood around the mouth while working was the differential risk factor between job categories among slaughterhouse workers.