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RT-LAMP를 이용한 콩황화일반모자이크바이러스의 진단
배대현, 이영훈, 김봉섭, 윤영남, 강범규, 최만수, 구성철, 김현태, 윤홍태, 이수헌, 백인열 忠北大學校 農業科學硏究所 2014 農業科學硏究 Vol.30 No.2
Soybean yellow common mosaic virus (SYCMV) has been recently reported, it has been occurred a lot with Soybean mosaic virus (SMV) and Soybean yellow mottle mosaic virus (SYMMV) in soybean field. SYCMV belongs to genus of Sobemovirus and induced viral symptoms with yellowing, mottle and mosaic. A reverse transcription loop-mediated isothermal amplification (RT-LAMP) method allowed one-step detection of gene amplification by simple procedure and needed only a simple incubator for isothermal template. This RT-LAMP method allowed direct detection of RNA from virus-infected plants without thermal cycling and gel electrophoresis. In this study, we designed RT-LAMP primers named SYCML-F3/B3/FIP/BIP from coat protein gene sequence of SYCMV. After the reaction of RTLAMP, products were identified by electrophoresis and with the detective fluorescent dye, SYBR Green I. under daylight and UV light. Optimal reaction condition was at 63 for 60min and the primers of RTLAMP showed the specificity for only SYCMV tested in this study.
일부 초등학생의 비만도에 따른 칼슘과 철의 섭취상태와 혈청 농도에 관한 연구
이화성,김은영,배윤정,승정자 숙명여자대학교 건강·생활과학연구소 2006 生活科學硏究誌 Vol.- No.22
The purpose of this study was to evaluate the nutrition status of calcium and iron in 116 elementary school students with different obesity index. Subjects were assigned to under weight, normal weight or over weight group according to the their obesity index. Their nutritional status, serum calcium and iron levels were evaluated based on 24-recall dietary recalls, anthropometric measurements and blood analysis. The mean height, weight, obesity index and blood pressure of total subjects were 150.4 cm, 45.5 kg, 5.8% and 118.2/76.8 mmHg. The mean % of body fat, SBP and WHR were higher while LBM and TBW were lower in over weight subject than those of other group. The iron intake of overweight was lower than that of underweight and normal subjects(p<0.05) and average calcium intakes were only 50% and 60% respectively of the RDA for Korean. Serum calcium and iron levels of a three groups were within the normal range. There were no significant differences in serum calcium and iron concentrations among three groups, however those of underweight group were a slightly lower than those of other two groups. There were significantly negative correlations between iron of intake and weight, obesity index, systolic pressure and body fat percent. Systolic blood pressure was negatively correlated to the intakes of energy, carbohydrate and protein. Energy intake was also negatively correlated to serum iron. To summarize the results, calcium and iron intakes should be supplied to elementary school students, especially those who were overweight. These indicated that overweight group had improper mineral nutrition status. Therefore, nutrition education and profound studies from many different focus for overweight groups are highly required.
( Yeong-bae Yun ),( Ji-hye Han ),( Young-kee Kim ) 한국미생물 · 생명공학회 2018 Journal of microbiology and biotechnology Vol.28 No.12
Pseudomonas tolaasii 6264 is a representative strain that causes bacterial blotch disease on the cultivated oyster mushroom, Pleurotus ostreatus. Bacteriophages are able to sterilize the pathogenic P. tolaasii strains, and therefore, they can be applied in creating disease-free mushroom cultivation farms, through a method known as “phage therapy”. For successful phage therapy, the characterization of phage-resistant strains is necessary, since they are frequently induced from the original pathogenic bacteria in the presence of phages. When 10 different phages were incubated with P. tolaasii 6264, their corresponding phage-resistant strains were obtained. In this study, changes in pathogenic, genetic, and biochemical characteristics as well as the acquired phage resistance of these strains were investigated. In the phylogenetic analyses, all phage-resistant strains were identical to the original parent strain based on the sequence comparison of 16S rRNA genes. When various phage-resistant strains were examined by three different methods, pitting test, white line test, and hemolytic activity, they were divided into three groups: strains showing all positive results in three tests, two positive in the first two tests, and all negative. Nevertheless, all phage-resistant strains showed that their pathogenic activities were reduced or completely lost.
Yun, Yeong-Bae,Park, Seong-Wan,Cha, Jae-Soon,Kim, Young-Kee The Korean Society for Applied Biological Chemistr 2013 Applied Biological Chemistry (Appl Biol Chem) Vol.56 No.1
Brown blotch disease of cultivated mushrooms is caused by Pseudomonas tolaasii, which secretes the bacterial toxin, tolaasin. Tolaasin is a peptide toxin that causes pore formation in the plasma membrane of mushroom cells. Forty-two strains of pathogenic bacteria causing brown blotch or similar diseases were isolated from mushrooms showing disease symptoms. To characterize these bacteria, the genes of 16S rRNA were sequenced and analyzed. Thirty-three strains were identified as five different species of Pseudomonas. Of these, 23 were identified as P. tolaasii and named as P1-type pathogens. Because the strains identified as P. tolaasii were major pathogens that cause the brown blotch disease, phylogenetic analyses of these pathogens were conducted by the neighbor-joining method. These strains comprised three phylogenetic subtypes, $P1{\alpha}$ (6 strains), $P1{\beta}$ (16 strains), and $P1{\gamma}$ (1 strain). Biological characterizations of the isolated bacteria were performed and confirmed that all three subtypes were able to cause the disease by forming blotches on the surface of the mushroom tissue. However, hemolytic activities were observed in the $P1{\alpha}$ and $P1{\gamma}$ strains, but not in the $P1{\beta}$ strains. These results imply that remarkable diversity exists among the various strains of P. tolaasii, each strain showing distinct biological characters.
Yeong-Bae Yun,Seong-Wan Park,차재순,김영기 한국응용생명화학회 2013 Applied Biological Chemistry (Appl Biol Chem) Vol.56 No.1
Brown blotch disease of cultivated mushrooms is caused by Pseudomonas tolaasii, which secretes the bacterial toxin,tolaasin. Tolaasin is a peptide toxin that causes pore formation in the plasma membrane of mushroom cells. Forty-two strains of pathogenic bacteria causing brown blotch or similar diseases were isolated from mushrooms showing disease symptoms. To characterize these bacteria, the genes of 16S rRNA were sequenced and analyzed. Thirty-three strains were identified as five different species of Pseudomonas. Of these, 23 were identified as P. tolaasii and named as P1-type pathogens. Because the strains identified as P. tolaasii were major pathogens that cause the brown blotch disease, phylogenetic analyses of these pathogens were conducted by the neighbor-joining method. These strains comprised three phylogenetic subtypes, P1α (6 strains), P1β (16 strains), and P1γ(1 strain). Biological characterizations of the isolated bacteria were performed and confirmed that all three subtypes were able to cause the disease by forming blotches on the surface of the mushroom tissue. However, hemolytic activities were observed in the P1α and P1γ strains, but not in the P1β strains. These results imply that remarkable diversity exists among the various strains of P. tolaasii, each strain showing distinct biological characters.
Yeong-Bae Yun,Dae-Hui Jeong,Hae-Yun Kwon,Yurry Um 한국응용생명화학회 2024 Journal of Applied Biological Chemistry (J. Appl. Vol.67 No.-
Angelica gigas Nakai belongs to the Unbelliferae family and was a representative medicinal plant with antiinflammatory, antioxidant and immunoenhancing activities. Recently, changes in the atmospheric environment, such as temperature, CO2 concentration and solar radiation, have continuously resulted in the reduction of cultivation area and production amount of A. gigas. They could affect the synthesis of secondary metabolites including nodakenin, decursin and decursinol angelate. This study aimed to investigate the atmospheric factors and the growth and content of active compounds of A. gigas. Air temperature and soil temperature were lowest in Jeongseon (northern region). Solar radiation was highest in Bonghwa (central region). Relative humidity was the lowest in Jeongseon from January to June, and it was the lowest in Bonghwa after July. At the Jeongseon experimental site, most of the growth characteristics of A. gigas were poor. The growth characteristics of A. gigas in Chungju and Bonghwa did not show any significant difference. The contents of active compounds were significantly highest in A. gigas collected from Chungju, but were significantly low in Jeongseon. Relative humidity showed a significant positive correlation with the content of the active compounds except for nodakenin. Decursin and decursinol angelate were significantly negatively correlated with solar radiation and soil moisture content, respectively. Most of growth characteristics of A. gigas, except for root length, showed a significant positive correlation with nodakenin content. The results of this study can be used as data for predicting the location of A. gigas cultivation according to the growth and active compounds of A. gigas in different cultivation regions.