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Phylogenetic Analysis of Pines Based on Chloroplast trnT-trnL Intergenic Spacer DNA Sequences
Um, Yurry,Park, Won-Kyu,Jo, Nam-Su,Han, Sim-Hee,Lee, Yi Institute of Forest Science 2014 Journal of Forest Science Vol.30 No.3
This study was conducted to distinguish the pines that are too similar to differentiate using conventional methods. Pinus densiflora and Pinus sylvestris have similar anatomical structure. They both have window-like pits and dentate ray tracheids, so it is not easy to distinguish the plants. We tried to find molecular markers by comparing chloroplast DNA sequences to differentiate the pines growing in Korea. We used P. densiflora, P. densiflora for. multicaulis, P. sylvestris, P. rigida, P. rigitaeda, P. koraiensis, and P. bungeana for this study. We found that the non-coding intergenic region of trnT(UGU) and trnL(UAA) genes have differences among the species. We designed a primer set to amplify the region efficiently and compared the PCR product sequences using CLC Workbench programs to find the polymorphism. We could distinguish the species using the sequences of the amplified region and the sequences were reproducible from the pines collected in Korea.
Genetic diversity analysis of Glycyrrhiza uralensis using 8 novel polymorphic microsatellite markers
Um, Yurry,Jin, Mei-Lan,Lee, Yi,Hur, Mok,Cha, Seon Woo,Jung, Chan Sik,Kim, Seong Min,Lee, Jeong-Hoon The Korean Society of Plant Biotechnology 2016 식물생명공학회지 Vol.43 No.2
Licorice plant (Glycyrrhiza spp.) is an important herb, but the major portion of the national demand is imported to Korea because the domestic production base is vulnerable. We performed basic molecular breeding research for domestic cultivation and production. All publicly available G. uralensis EST sequences, which totaled 56,089, were assembled into 4,821 unigenes and examined for microsatellites. Eight polymorphic microsatellite loci were identified and 16 G. uralensis and 6 G. glabra accessions, which were collected from different locations, were genotyped using the microsatellites. Genetic diversity within the accessions was estimated by construction of a dendrogram. The dendrogram was clustered into two groups. The results showed that there is a correlative genetic relationship between species. The microsatellite markers were found to be useful for diversity analysis as they are able to successfully distinguish the Glycyrrhiza accessions.