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효소고정화막과 반투막성질의 PVA적층막 제조 및 산소센서에서의 특성
서종원,김태진,정용섭,윤정원 경북대학교 센서기술연구소 1998 센서技術學術大會論文集 Vol.9 No.1
The oxygen electrode of biosensor needs enzyme immobilized membrane and dialysis membrane for measurement of oxygen concentration, which in solution after enzyme reaction with its substrate. The one-layer PVA laminated CTA/PCL membrane was developed for oxygen electrode of biosensor. Enzymes have immobilized on cellulose triacetate/polycarprolactone membrane by CDI method, and laminated with polyvinyl alcohol, aldehyde and acid. This membrane immobilized with glucose oxidase, ascorbate oxidase, pyruvate oxidase and alcohol oxidase by CDI mehod, and PVA laminated CTA/PCL membrane have been tested with various concentration of substrates of enzymes by use of YSI oxygen sensor. Under 5-10mmol substrates become 0.37-0.83μA(r=0.995) currents, and about 56% of glucose oxidase activity was remained after 8 weeks, others were remained very low. SEM shows smooth surface and tightly attached PVA on enzyme immobilized CTA/PCL membrane.
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정진일,김기영,최윤희,최원영,고종철,오명규,홍하철,이승엽,이명철 한국국제농업개발학회 2004 韓國國際農業開發學會誌 Vol.16 No.4
평야지재배 적응 조생종 벼 품종의 선발 및 품종 육성의 기초 자료로 활용코자 극조생종(1품종)과 조생종(10품종) 및 준조생(3품종)등 총 14품종을 산간지(운봉)와 평야지(익산)에 지역별 표준재배법으로 공시한 결과, 1. 극조생종 및 조생종들은 감온성이 커 산간지에서 출수가 빠르나, 준조생종에 속하는 상미벼, 영덕34호, 히또메보레 등은 감광성이 감온성보다 커, 평야지재배에서 4일정도 빨리 출수하였다. 2. 수량성은 대부분의 품종들이 산간지재배에 비해 평야지 재배에서 높았고 대체로 출수가 빠른 품종일수록 지역간의 수량차가 크게 나타났으며, 출수가 다소 늦은 준조생종들은 산간지와 평야지의 재배에서 수량차가 크지 않아 산간지 뿐만 아니라 평야지에서도 적응성이 높았다. 3. 단백질함량은 숙기가 다소 늦은 품종일수록 낮은 경향을 보였고 산간지보다는 평야지에서 낮았으며, 품종간에는 오대벼, 운봉30호, 영덕34호, 신석조생, 상미벼 등이 함유량이 낮았다. 4. 식미치는 산간지재배가 평야지에 비해 보다 높게 나타났고, 출수가 늦을수록 높았으며, 지역간 차가 크게 나타난 품종은 극조생종인 키라라397이었고, 출수가 늦은 상미벼와 히또메보레는 차가 적었다. 5. 산온도와 수량과의 상관은 산간지에서만 영향이 있을 뿐 평야에서는 영향이 적고 일사량과 일조시수가 수량에 보다 상관성이 높았으며, 식미치는 적산온도와는 부의상관, 생육 일수와는 정상관을 보였다. This experiment was carried out to clarify the difference of rice yield and the quality of rice in southern plan area and in alpine area. Yield and quality of fourteen early varieties were investigated under the different transplanting dates and planting densities in southern plan area(Iksan) and in alpine area(Unbong). In the alpine area, extremely early variety and early varieties headed early compared to the plain area, but medium early varieties headed about 4 days late unlike the alpine area. The yield difference of most early varieties including Kilara 397 of extremely early flowering variety were greater in plain area than in alpine. The different of yield between plain and alpine area was less in varieties with Sinseogjosaeng, Odaebyeo, Akidagomachi, and Yeungdeuk34. The rate of protein was lower in plain area than alpine and more lately flowering than early, but palatability of milled rice was higher at the alpine area and more lately flowering varieties. Correlation coefficients between yield and meterological data during maturity, yield shower the significant correlation with integrated solar radiation and sunshine hours at cultured in southern plan area, but it showed only cumulative temperature in alpine area. Palatability of miller rice was positive correlation at growth duration but negative correlation at cumulative temperature.
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Antioxidative and Anti-inflammatory Activities of Litsea japonica Leaves
Yoon, Weon-Jong,Kang, Se-Chan,Ham, Young-Min,Kim, Kil-Nam,Yang, Weon-Hyuk,Kim, Hee-Jung,Park, Soo-Yeong,Jung, Yong-Hwan The Korean Society for Applied Biological Chemistr 2010 Applied Biological Chemistry (Appl Biol Chem) Vol.53 No.1
We investigated the antioxidative and anti-inflammatory properties of an 80% ethanol extract and solvent fractions of Litsea japonica leaves. The extract and the $CH_2Cl_2$, EtOAc, and BuOH fractions showed antioxidant potential, based on radical-scavenging activity. In LPS-stimulated RAW 264.7 cells, the $CH_2Cl_2$ fractions inhibited nitric oxide (NO) and prostaglandin $E_2(PGE_2)$ production. Additionally, the $CH_2Cl_2$ fractions inhibited inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression. These results show that the $CH_2Cl_2$ fractions of L. japonica strongly inhibited the production of NO and $PGE_2$ and the induction of iNOS and COX-2 in LPS-stimulated cells. Thus, we propose that $CH_2Cl_2$ fractions may inhibit NO and $PGE_2$ production primarily through the regulation of iNOS and COX-2 gene expression. This study suggests the L. japonica may be a natural source of effective antioxidant and anti-inflammatory agents.
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Inhibitory Effects of Ficus erecta Leaves on Osteoporotic Factors In Vitro
Yoon, Weon-Jong,Lee, Hye-Ja,Kang, Gyeoung-Jin,Kang, Hee-Kyoung,Yoo, Eun-Sook 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.1
Osteoporosis is recognized as a major concern among menopausal women and the elderly. When estrogen is reduced in the body, local factors such as $IL-1{\beta}$, IL-6 and $PGE_2$, which are known to be related to bone resorption, are increased and promote osteoclastogenesis, which is responsible for bone resorption and results in the clinical disorder osteoporosis. In this study, we investigated the anti-osteoporotic activity of Ficus erecta. MG-63 cells were stimulated with $IL-1{\beta}(10ng/mL)$ to induce osteoporotic factors (IL-6, COX-2 and $PGE_2$) and RAW 264.7 cells were stimulated with RANKL (100ng/mL) to induce their differentiation into osteoclasts. We found F. erecta fractions decreased the mRNA expression of IL-6 and COX-2, and protein levels of COX-2 and $PGE_2$ production. Among sequential solvent fractions, hexane and EtOAc fractions decreased differentiation into osteoclasts of RAW 264.7 cells. These results suggest that F. erecta may have significant effects on osteoporotic factors and may be provided as a possible anti-osteoporotic therapeutic plant.
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