Molecular identification of the strains of the domestic silkworm, Bombyx mori (Lepidoptera: Bombycidae), which are endemic to Korea, based on single nucleotide polymorphisms in mitochondrial genome sequences

Park Jeong Sun,Kim Min Jee,Kim Seong-Wan,Kim Kee-Young,Kim Seong-Ryul,김익수 한국응용곤충학회 2022 Journal of Asia-Pacific Entomology Vol.25 No.2

The domestic silkworm, Bombyx mori (Lepidoptera: Bombycidae), has been diversified into various strains over a long period. However, methods to distinguish silkworm strains remain limited partially owing to the genetic similarity caused by the long history of domestication. In this study, we developed molecular identification methods to distinguish three domestic silkworm strains, which are endemic to Korea. By comparing publicly available complete mitochondrial genome (mitogenome) sequences of five endemic strains and 34 stock silk worm strains analyzed in a previous study, we detected 15 single nucleotide polymorphisms (SNPs; SNP1–SNP15), which distinguished the following three endemic strains: Sun7ho (SN7), Sandongsammyeon (SDS), and Sammyeonhonghoeback (SMH). We used two SNPs for each strain to identify the three endemic strains. To distinguish each SN7 and SDS from the remaining four endemic and 34 stock strains, the PCRrestriction fragment length polymorphism method was employed using Acu I and Hpa I restriction enzymes, which recognize SNP1 and SNP8, respectively. Additionally, the tetra-primer amplification refractory mutation system PCR method was used to determine the regions containing SNP3, SNP11, and both SNP14 and SNP15 to distinguish SN7, SDS, and SMH, respectively, from the remaining strains. A validation test with additional in dividuals showed that each target strain was clearly recognized, suggesting that mitogenome SNP-based methods can be used to identify three endemic silkworm strains during culture and breeding.

고지방식을 섭취한 랫드에서 김치유산균의 콜레스테롤 저하효과

조미선, 노정미, 임형준, 한선경, 신명수, 이완규 충북대학교 동물의학연구소 2006 Journal of Biomedical and Translational Research Vol.7 No.2

본문참고

심실보조장치 이식의 새로운 수술기법-Korea University Technique-

선경,박성영,오혜정,신재승,이혜원,심환주,김형묵 제주대학교 인공심장이식연구소 2001 인공심장 연구 Vol.2 No.1

고려대학교 흉부외과에서는 심실보조장치 이식에서 흉골 재절개(re-sternotomy)에 따른 합병증과 사망률을 감소시키기 위하여 우측 개흉을 이용한 독자적인 수술법을 개발하였다 (KU technique). 다양한 동물모델과 사체실험 및 임상적용을 통해 안정성과 유용성을 확인하였으며, 향후 좌심 및 양심보조장치 이식에서 표준기법의 하나로 제안하고자 한다. We have developed a new surgical technique (KU technique) of right thoracotomy for cannulation of ventricular assist devices, which can lessen potential morbidity and morta1ity from re-sternotomy. The technique has been proved to be safe and efficient in various animal models, preclinical cadaver fitting test, and human application. We would like suggest KU technique as a part of standard in implantation of left ventricular or hi-ventricular assist devices.

조명조건 변화에 따른 횐쥐 시각교차위핵 신경세포의 면역반응성과 유전자 발현의 변화

백선용,김완수,김진정 대한해부학회 1995 Anatomy & Cell Biology Vol.28 No.6

강원대학교 연습림내 蓮葉山과 九節山地域의 植物相에 관한 硏究

朴完根,廉圭眞,朴善貞 江原大學校 森林科學硏究所 1997 Journal of Forest Science Vol.13 No.-

要約강원대학교 연습림의 연엽산과 구절산 지역을 대상으로 植物相을 조사한 결과, 管束植物資源은 98科 345屬 551種 2亞種 88變種 10品種 2雜種으로 총 653분류군이 분포하는 것으로 나타났다. 이들 所産植物중 韓國特産植物은 20種類, 그리고 稀貴 및 滅種危機植物은 18種類가 분포하였으며, 自然生態系에 예측할 수 없는 영향을 초래하는 歸化植物은 27種類가 출현하였다. 귀화식물 種類수에 의한 自然破壞度(UI)는 약 12.4%로 나타났다. 또한 所産植物들의 有用度는 食用資源(42.1%), 藥用資源(33.8%), 牧草用(28.8%), 觀賞資源(11.8%), 木材資源(5.2%), 纖維資源(1.4%), 工業用(1.1%) 등의 순으로 나타났으며, 아직 용도가 밝혀지지 않은 植物資源도 140種類(21.4%)로 나타났다.ABSTRACTThis study was to survey the flora of resources vascular plants in the Experiment Forests of Kangwon National University. Resources vascular plants were composed of 98 families, 345 genera, 551 species, 2 subspecies, 88 varieties, 10 formae, and 2 hybrid, totaling 653 taxa. Among the investigated resources plants, Korean endemic plants and rare or endangered plants were distributed 20 taxa and 18 taxa, respectively. There were 27 taxa of Naturalized plants in this study area, which would have unpredictable influence on the natural ecosystem. The urbanized index(UI) of all over the investigated areas was about 12.4%. Useful resources plants were composed edible source(42.1%), medicinal source(33.8%), pasture source(28.8%), ornamental source(11.8%), timber source(5.2%), fiber source(1.4%), industrial source(1.1%). Vascular plants of unknown use were 140 taxa(21.4%)

다짐도에 따른 화강풍화토의 역학적 특성에 관한 연구

주승완,김선학 서강대학 지역발전연구소 2004 지역발전연구 : 서강전문대 Vol.10 No.-

In this study do we collect the Wethered Granite Soil of Gwang-ju Pung -am district and Youm-ju-dong Gwang-ju world cup stadium construction field and identify physical character according to basic physical test and summerged the sample of 85% degree of compaction in the dry side and wet side in a water tank for ten days on the basis of the sample of 80%, 85%, 90% degree of compaction and Optimum Moisture Ratio in the Optimum Moisture Ratio state in order to exam permeability and compression character of the Weathered Granite Soil according to the compaction state of retaining wall, abutment bankfill banking. We perform falling head permeability test and them standard compaction test and calculate coefficient of permeability and consolidation constant according to the test result. We have a mind to clear permeability and compression character of the Weathered Granite Soil of this study area according to relation curve of the calculated coefficient of permeability, consolidation constant and void ratio, degree of compaction. Therefore compression constant, according to the compaction energy of Optimum Moisture Ratio, is in inverse proportion linearally in the Weathered Granite Soil of WGS 1 and WGS-2 and coefficient of permeability increase directly in a range of void ratio e=0.627~0.779, e=0.657~0.800.

연약지반상 성토시 측방변위 거동에 대한 유한요소해석

주승완,김선학 서강전문대학 지역발전연구소 2003 지역발전연구 : 서강전문대 Vol.9 No.-

If we embank on the soft ground, to construct road and other structures, Settlement and lateral displacement in the subground develop during and after the construction of embankment. Because lateral displacement and settlement in the ground can affect close structure and piles and used as important measure data indeciding safety of the embankment frame, it is important precisely to forecast the size and distribution of lateral displacement and settlement. Therefore, in this study, to analyze lateral displacement according to construction and ground condition, practiced two dimentional finite element analysis using the finite element analysis program SAGE CRISP 4.02. Embankment condition is the change of embankment decline and velocity, Ground condition is the change of drainage condition, OCR and permeability coefficient and the mixture of over consolidation layer and sand layer in the ground. As a result of analysis, whole lateral displacement increase inconstant velocity before embankment completion and after embankment completion, lateral displacement velocity decrease and is very small in 1000days. Besides, maximun lateral displacement in the embankment frame hem is restrained according as hardness of the ground is large and drainage well occurs.

4주간의 쥐뒷다리 고정이 Soleus근섬유 조성에 미친 영향

이선장,김완조,김문희 韓國體育大學校附屬 體育科學硏究所 1992 韓國體育大學校附屬 體育科學硏究所論文集 Vol.11 No.1

The purpose of this study was to examine the effect of immobilization on muscle fiber composition, mass, area and capillarization after 4 - wk of hindlimb suspension. Significant reductions in soleus mass, area and capillary density were occured after 4 wks of hindlimb suspension, Wheres type IIa fiber percentage was significantly increased in the experimental group. The results suggest that hindlimb unweighting induced soleus muscle atropy and these results also demonstrate that muscle fiber atropy not necessarily being associated with a loss in capillary supply.

왕가시오가피나무 잎의 성분

박유선,함인혜,황완균 중앙대학교 약학연구소 1997 약학 논총 Vol.11 No.-

Constituents from the leaves of Acanthopanax senticosus var. Koreanus(Araliaceae), a plant origin of Acanthopanax senticosi Cortex which has been used as tonic, stress-relaxing agent, antidiabetes, antirheumatic, etc. in Korean folk medicine were isolated. The methanolic extracts of dried leaves of Acanthopanax senticosus var. Koreanus was suspended in ether to remove fatty substances. The aqueous fraction was further seperated by Amberlite XAD-2 column employing gradient solvent systems(H_2O, 20, 40, 60, 100% MeOH). By successive column chromatography using sephadex LH-20, ODS and polasil gel. Compound Ⅰ and Cpmpound Ⅱ, were isolated from the 40% MeOH fraction. Chemical structures of Compound Ⅰ and Ⅱ were identified as chiisanoside, and quercetin-3-β-D-galactopyranoside respectively on the basis of physico-chemical and spectral data of IR, FAB-MS, ^1H-NMR, ^13C-NMR, ^1H-^1H COSY, ^13C-^1H COSY and HMBC(^1H-Derected Multiple-bonded Heteronuclear quantum Coherence spectrum).

후매립 단일앵커의 강도특성

박완신,황선경,윤현도 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.2(구조계)

Many models for the design of post-installed steel anchors are presented in this paper. These approach are compared to each other, especially the well-known provisions of CEB method, ACI 349-90 and the CCD method. The use of these methods to predict the concrete failure load of fastenings in uncracked concrete under monotonic loading for important applications is compared. Variables included single anchors away from and close to the edge, embedment depth, concrete strength, tension loading, and shear loading. A data bank including Korean, European and American tests was evaluated. The comparison shows the CCD Method and CEB Method can accurately predict the concrete failure load of fastenings of the full range of investigated applications. On the other hand, depending on the application in question, the predictions of ACI 349-90 and VAC Method are sometimes unconservative and sometimes conservative.