잡초성벼의 superoxide dismutase cDNA cloning과 재배벼로의 형질전환

박상규,박종석,이승인,서석철,김병극,조윤래,서학수 한국환경농학회 2002 한국환경농학회지 Vol.21 No.2

냉해나 한발등의 환경스트레스에 대해 저항성을 유발하는 유전자를 환경스트레스에 강한 잡초성벼로부터 선발하고 이들 유전자를 재배벼에 도입하여 도입유전자 산물의 과량 발현을 통해 냉해나 한발 등에 대한 저항성이 향상된 벼를 선발하고자 하였다. 잡초성벼인 Bhutan 14Ad로부터 냉해 및 한발 저항성 유전자로 알려진 superoxide dismutase (SOD) cDNA를 분리하고자 mRNA를 분리하고 이 분리된 mRNA를 이용해 reverse transcriptase PCR방법으로 SOD cDNA를 cloning 하였다. 그 결과 2종의 SOD cDNA가 cloning되어 SOD-A, SOD-B로 명명하였다. 이들 cDNA의 염기서열을 결정한 결과 이들은 아미노산 서열 상동성이 88.4%를 나타내었으며, SOD-A는 oryza sativa 계열의 Cu/Zn SOD유전자인 GenBank accession No. L36320와 99.3%로 동일하였으며, SOD-B는 accession No. D01000과 100% 동일하였다. 이들 SOD-A와 SOD-B cDNA를 재배벼인 낙동벼에 형질전환하여 형질전환체 벼를 선발하였으며, 이들 형질전환체 벼의 냉해저항성 및 한발저항성 검정을 통해 저항성이 향상된 형질전환체 벼를 선발하고 있다. Two different cDNA clones for superoxide dismutase (SOD) were isolated from an weedy rice variety (Oryza sativa, cv. Bhutanl4Ad) and were introduced into a cultivated rice variety (Oryza sativa, cv. Nakdong) in order to develop the environmental stress-resistant rice plants. Sequence analysis of the cloned cDNAs indicated that the deduced amino acid sequence of SOD-A is 88.4% identical to that of SOD-B. Furthermore, the nucleotide sequence of SOD-A is 99.3% identical to that of a Cu/Zn SOD gene of Oryza sativa (GenBank accession No. L36320). The nucleotide sequence of SOD-B was identical to that of the previously published SOD gene (Accession No. D01000). A cultivated rice variety, Nakdong-byeo, was transformed with chimenc SOD genes containing a actin promoter of rice and pin2 terminator using a particle bombardment technique. Transformed calli were selected on an selection medium containing phosphinothricin (PPT). Transgenic rice plants were regenerated from the PPT-resistant calli. PCR analysis with genomic DNAs from transgenic plants revealed that transgenes are introduced into rice genome.

Comparative Genomic Hybridization 기법을 이용한 한국인 원발성 간세포암에서의 염색체 변화에 대한 연구

박훤겸,정풍만,이광수,조율희 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.1

Comparative genomic hybridization(CGH) technique is one of the screening tests for DNA sequence copy number changes(losses, deletions, gains, amplifications) in tumor genomes. DNA changes in 23 hepatocellular carcinomas(HCC) associated with hepatitis B virus(HBV) were analyzed by CGH technique. Eighteen of the 23 cases showed genetic alterations. The remaining 5 cases showed no detectagble aberration. The losses of chromosome regions 17p(74%), 4q (57%), 16p (52%), 16q (48%), 8p (43%) and 13q (43%) were detected in the order of decreasing frequency. In cases of multiple losses of chromosomes, a combination of 17p,16p,16q,4q, and 8p losses was found in 5 cases (30%). On the other hand, chromosomal gains occurred on 1q (65%), 8q (52%), 20p (48%) and 20q (43%) in the order of decreasing frequency. And the simultaneous genomic gains of these 4 chromosomes were found in 9 cases (40%). Moreover, the combination of 5 genomic losses (17p, 16p, 16q, 4q, & 8p) and 4 genomic gains (1q, 8q, 20p, & 20q) was observed in 4 case s(23%). The pattern of chromosomal gains and losses in HCC with hepatitis B in Korean patients is very complex and involves virtually every site in the genome. This indicated a high genomic instability. This could possibly be explained either as the result of random chromosomal changes during early development of tumor, or as the highly variable and random pattern of integration of HBV in the HCC. The hepatocarcinogenesis may be the result of cumulative effocts rather than those orders of occurrence of those genomic changes. The sites of cellular DNA at which HBV integrates frequently undergo rearrangements, resulting in translocations, inverted duplications, deletions, and possibly recombinational events. But, CGH only detects changes of chromosomal copy number but could not identify translocations, inversions, and other aberrations of chromosome. The chromosomal analysis of HCC with HBV in Korean patients by CGH technique confirms the presence of complex and sporadic, but nonrandom genetic changes in the chromosome. In the future, more detailed oncogenic study could be carried out on the chromosomes which showed abnormal aberrations through this study.

렙토스피라증의 진단에 있어 수동혈구응집반응법과 육안응집반응법의 정확도에 관한 연구

최보율,김영규,이수진,송재철,박항배 한양대학교 의과대학 1994 한양의대 학술지 Vol.14 No.2

Before 1984, Leptospirosis had been reported sporadically in middle inland of Korea as hemorrhagic pneumonia like disease, epidemic pulmonary hemorrhagic fever or epidemic pneumonitis since 1975. The epidemiologic characteristics of this disease were found out and the agent was isolated microbiologically after a large epidemic in 1984. Thereafter many consecutive studies for leptospirosis had been performed in the realm of epidemiology, microbiology and clinical field. But in order to appropriate control of Leptospirosis there are many problems such as organizing surveillance system for early diagnosis and treatment, identifying sero-group and serovar in each epidemic area and assessing vaccine efficacy. The objectives of this study was to find out easily applicable diagnostic methods to be used in a rural community surveillance system. Two diagnostic methods, passive hemagglutination(PHA) test and macroscopic agglutination test(maAT) were measured against their accuracies, sensitivity, specificity and predictive value of positive and negative. 301 sample sera from 270 human cases was collected through Leptospirossis surveillance system in a rural community during 1989-1992. Microscopic agglutination test(MAT) were used for confirmed diagnosis according to WHO guideline with 4 strains; Icterohemorrhagiae lai and Canicola canicola Hond Utrecht IV as standard strians and 6P-014-1 and YP-35-1 as strains isolated in Korea in 1986. The results are as follows. 1. 75(24.9%) among 301 sample sera and 49(18.1%) among 270 persons was serologically positive with MAT. 2. Agreement between PHA and MaAT was very low(k-coefficient≤0.252). 3. In PHA, specificity was very high(≥0.96) but sensitivity was very low(≤0.28). 4. In MaAT, specificity(≥0.73) was relatively lower than PHA but sensitivity (≥0.79) was significantly higher than PHA, especially false-positive rate case was absent in MAT's high antibody titer(above 1:640). It was concluded that MaAT method was a very useful diagnostic tool for early diagnosis of Leptospirosis in a rural community surveillance system, provided that confirmed diagnosis with MAT method be carried out in laboratory of medical school or research institute such as National Institute of Health or Provicial government Institute of Health and Environment.

넙치, Paralichthys olivaceus의 수온 자극 스트레스에 대한 사료첨가제 투여 효과

권문경,박상언,방종득,조병열,이상민,박수일 한국어병학회 2003 한국어병학회지 Vol.16 No.3

본 연구는 넙치의 수온 스트레스에 대한 사료첨가제 (미역 5%, 미역 10%, 어보산 0.5%, 고추냉이 잎 2%, 고추냉이 줄기 2%)의 영향을 혈액학적, 면역학적 측면과 Edwardsiella tanda를 사용하여 질병에 대한 저항성으로서 평가하였다. 혈장의 글루코즈와 콜티졸 농도는 1차 수온 변동 후 대조구에 비하여 미역 5%와 고추냉이잎 2% 첨가구에서 유의적으로 낮게 나타났다(P〈0.05). 1차 수온 변동 후 혈장 라이소자임 활성과 E. tarda의 공격 시험 후 생존율은 미역 5%첨가구에서 대조구에 비하여 유의적으로 높게 나타났다(P〈0.05). 본 실험 결과 미역 5% 첨가 사료 투여는 넙치의 스트레스 상태에서 질병 감염에 대한 저항성을 높일 수 있을 것으로 사료된다. The effect of each dietary supplements (Undaria 5%, Undaria l0%, Obosan 0.5%, Wasabi leaf 2%, Wasabi stem 2%) on the water temperature fluctuation in juvenile olive flounder (Puralichthys olivaceus) was investigated. The response to stress was assessed in terms of effects on haematological and immunological, and resistance against Edwurdsiella tarda infection. Plasma glucose and cortisol levels were significantly lower in 5% undaria and 2% wasabi leaf supplement groups than the controls after the first change of water temperature (P<0.05). The plasma lysozyme activities and the survival rates from E. tarda infection were significantly higher in the 5% undaria supplement groups than the control (P<0.05). These results suggest that the 5% undaria supplement seems to be contributable to the increased disease resistance on olive flounder.

素問·評熱病論에 대한 硏究

千相默,張鍾烈,琴坰樹,朴炅 圓光大學校 韓醫學硏究所 1997 원광한의학 Vol.7 No.1

『素問·評熱病論』은 본래 正氣가 虛弱한데다 風熱의 邪氣에 感觸되어 발생하는 陰陽交·風厥 등의 熱病과 熱病의 勞風·腎風 等의 病理 治療 豫候등을 자세하게 論述하고 있으므로 「評熱病論」이라고 하였다. 『內徑』이후에 陰陽交·風厥·勞風·腎風 等에 대하여 論한 『諸病源候論』, 『備急千金要方』, 『外臺秘要』, 『醫心方』, 『傷寒補亡論』, 『傷寒百證歌』, 『醫疊元戒』등 모든 醫書들은 本 篇의 내용을 인용하여 疾病의 病理 및 治療와 豫後를 파악하는데에 根幹으로 삼고 있다. A study on the Pyung Youl Byung Ron of the So Moon, one of the classic book of oriental medicine, was carried out analytically for the right comments. The principal obtained summarized as follow; The Pyung Youl Byung Ron is in chapter 33, Volumn 9 of the Hwang Jae Nae Guong.『Pyung Youl Byung Ron』 says Youl Bung(熱病) of Omyang Gyo(陰陽交), Pung Gyul(風厥), Lo Pung(勞風), Sin Pung(腎風), which come out when a fair and equitable spirit is under the power of poisonous energy and so weak and it is Pung Youl Byung Ron. This Book consist of four parts. The fisrt part explain 'Omyang Gyo'. The second part explain 'Pung Gyul'. The third part explain 'Lo Pung'. The forth part explain 'Sin Pung'. After 『Nae Gyung(內徑)』, 『Jae Buung On Hu Ron(諸病源候論)』, 『Bi Gub Chun Gum You Bang(備急千金要方)』, 『Yoi Dae Bi Yo(外臺泌要)』, 『Sang Han Back Dung Gs(傷寒百證歌)』, 『O Chub One Yung(醫疊元戒)』. This al medical Books take a line from Pyung Youl Byung Ron.

Synthetic route of the leuco compounds and their near-infrared absorption behaviour

Park, Soo-Youl,Jun, Kun,Oh, Sew-Wha 한국공업화학회 2003 응용화학 Vol.7 No.2

Although the 1,3-bis(4-aminoaryl)squaraines showed no pH-induced colour change behaviour, they were found to undergo reduction with sodium borohydride in solution to give colourless leuco compounds, which oxidised readily in air back to the coloured squaraine dye. This colour-change redox behaviour has potential in the area of peroxidase-based boiassays. The structures of the leuco compounds were investigated in some detail. Thus the hydroxy group could be (a) methylated with diazomethane to form O-methyl leuco compounds, (b) acetylated with acetic anhydride to give O-acetyl derivatives, and (c) reacted with phosphorous oxychloride or thionyl chloride to give chloro-leuco derivatives. The susceptibility of the chorine atom towards nucleophilic substitution in the last group of compounds was demonstrated by their facile reaction with alkylamies to give amino-leuco squaraines. The enolic nature of the hydroxy group also enabled a glycoside and a phosphate ester derivative to be made, which were of potential interest as chromogenic substrates for glycosidase and phosphatase enzymes respectively.

The synthesized of the antioxidant and evaluated the oxidation stability for biodiesel

Soo-Youl Park,Seung-Rim Shin,Joung-II Shin,Kyoung-Lyong An,Kun Jun 한국트라이볼로지학회 2013 한국트라이볼로지학회 학술대회 Vol.2013 No.10

Synthesis of Colour Changeable Dyes Based On Donor-Acceptor Chromophore

Park, Soo Youl,Oh, Sew Wha 한국공업화학회 2002 응용화학 Vol.6 No.2

Squarylium, or squaraine, dyes are derived from 1,2-dihydroxycyclobuten-3,4-dione, otherwise known as squaric acid. They are of two principal types: the 1,2-bis-donor substituted derivatives, and the 1,3-bis-donor substituted derivatives. The former is essentially merocyanines and have no distinctive properties, whereas the latter represent a unique type of chromophore, which is neither a merocyanines nor cyanine, and has exceptional light absorption characteristics. In this respect, types can be classified along with the H-chromophores as special cross-conjugated electron systems. They have many functional applications based on their special properties. Thus it was the objective of this research project to synthesise a range of 1,3-squarylium dyes with electron donor end groups based on heterocyclic residues, and to investigate their light absorption and fluorescence properties. During this study, a various pH-induced colour change processes, and the properties of appropriate examples in particular were examined.

The synthesized of the phenylenediamines substituted and evaluated the oxidation stability for biodiesel

Soo-Youl Park,Seung-Rim Shin,Joung-Il Shin,Kyoung-Lyong An,Kun Jun,Ho-Young Song,Ki-Ho Kim 한국트라이볼로지학회 2013 한국트라이볼로지학회 학술대회 Vol.2013 No.4

Colour Change Redox Behaviour of the 1,3-Squaraine Dyes

Park, Soo-Youl,Oh, Sew-Wha,Griffiths, J. 한국공업화학회 2002 응용화학 Vol.6 No.1

The 1,3-bis(4-aminoaryl)squaraines showed colour change behaviour, they were found to undergo reduction with sodium borohydride in solution to give colourless leuco compounds, which oxidised readily in air back to the coloured squaraine dye.We have investigated this possibility, and have found that reduction can, in most cases, be achieved readily in organic solvents with sodium borohydride, and moreover, the resultant colourless reduced species (leuco dyes) are generally chemically stable and can be oxidised back to the coloured dye with air or common inorganic oxidants. This meant that the 1,3-squaraines could be used as redox indicators, with possible functional applications in enzyme assays, oxygen detection, or reprographic systems, and thus the chemistry of the leuco-1,3-squaraines was investigated in detail in order to determines their potential value in such areas.