서울 단일 지역의 Helicobacter pylori 1차 제균율과 2차 제균율의 변화

김미연,심기남,김혜인,강현주,류민선,안소영,정혜경,정성애 Ewha Womans University School of Medicine 2014 EMJ (Ewha medical journal) Vol.37 No.1

Objectives: To investigate the rate of first-line eradication and the rate of second-line eradication of Helicobacter pylori (H. pylori) from 2001 to 2010 in a single institute in Seoul. Methods: Among the 2,717 patients who received H. pylori eradication treatment from 2001 to 2010 at Ewha Womans University Mokdong Hospital, the medical records of 1,466 patients who satisfied the condition of execution of upper gastrointestinal endoscopy, positive H. pylori eradication results at 6~8 weeks after eradication therapy were reviewed retrospectively. Then the first-line and second-line eradication rates and the eradication rates according to endoscopy findings were also compared. Results: The first-line eradication rate was 77% H. pylori eradication rate for the last 5 years was continuously increasing and no sign of decline was observed even for the whole 10 years. The rates of eradication related to endoscopic findings showed statistical significance (P<0.001) of 79.8% and 70.1% each for peptic ulcer and non-ulcerative gastric diseases, respectively. Conclusion: In this study, no decrease in tendency of first-line eradication rate could be found. In addition, the patients with the non-ulcerative gastric disease seemed to show significantly lower eradication rate. This finding suggests eradication treatment may be affected by the category of gastric diseases, and careful considerations should be taken assessing the effects and needs for the H. pylori eradication treatment.

한열변증에 따른 중풍 환자의 제반특성 비교

김민경,이인환,신애숙,김나희,김혜미,심소라,나병조,조승연,박성욱,정우상,문상관,박정미,고창남,조기호,김영석,배형섭,Kim, Min-kyung,Lee, ln-whan,Shin, Ae-sook,Kim, Na-hee,Kim, Hye-mi,Shim, So-ra,Na, Byung-Jo,Cho, Seung-yeon,Park, Seong-uk,Jung, Woo Sang 대한중풍순환신경학회 2010 대한중풍.순환신경학회지 Vol.11 No.1

Object : This study was conducted as part of the national project to standardize stroke diagnosis in Korean medicine. In this study, we aimed to evaluate the characteristics of acute stroke between heat pattern group and cold pattern group. Methods : We recruited stroke patients from 5 universities(Kyung-Hee University oriental medical center, Kyung-Hee University East-West Neo medical center, Kyungwon university lncheon oriental medical center, Kyungwon university Songpa oriental medical center and DongGuk university llsan oriental medical center) from April, 2007 until February, 2010. We diagnosed them and selected 463 heat pattern patients and 182 cold pattern patients. Results : We find that the risk factor of smoking, alcohol, diet(prefer to meat) are more associated with the heat pattern group. On the other hand, inflammation history in recent 3 months and diet(prefer to sea food) are more associated with the cold pattern group. RBC, Hg, Hct, TG, CK and Cl are more related to the heat pattern group. CPT, total cholesterol and HDL are more related to the cold pattern group. Tae-eum type takes high distribution of the heat pattern group, and So-eum type takes high distribution of the cold pattern group. Conclusions : This study provides evidence that the heat pattern group have more risk factor than the cold pattern group.

Antioxidative Properties of Onion Peel Extracts against Lipid Oxidation in Raw Ground Pork

Shim, So-Yeon,Choi, Yun-Sang,Kim, Hack-Youn,Kim, Hyun-Wook,Hwang, Ko-Eun,Song, Dong-Heon,Lee, Mi-Ai,Lee, Ju-Woon,Kim, Cheon-Jei 한국식품과학회 2012 Food Science and Biotechnology Vol.21 No.2

Onion peels extracted with various concentrations of ethanol were examined for their extraction yield, total phenolic content, DPPH scavenging activity, and reducing power. Onion peel extracted with 50% ethanol exhibited higher extraction yields, total phenolic content, and DPPH scavenging activity than extracts from distilled water, 70 or 95% ethanol. Thus, the antioxidative efficiency to inhibit lipid oxidation of ascorbic acid (0.05%) and 50% ethanol extract of onion peel (0.05, 0.1, and 0.2%) in raw ground pork was investigated during chilled storage (16 days). The pH of the samples decreased considerably during storage (p<0.05). Addition of ethanol onion peel extract significantly improved the thiobarbituric acid reactive substances and peroxide values in raw ground pork meat (p<0.05). Therefore, onion peel extract by 50% ethanol is a very effective inhibitor of lipid oxidation and has potential as a natural antioxidant in raw ground pork.

Neurodevelopmental Outcomes at 18–24 Months of Corrected Age in Very Low Birth Weight Infants with Late-onset Sepsis

Shim So-Yeon,Cho Su Jin,Park Eun Ae 대한의학회 2021 Journal of Korean medical science Vol.36 No.35

Background: Preterm infants are prone to sepsis owing to their immature innate immunity and prolonged hospitalization. We aimed to evaluate the association between late-onset sepsis (LOS) during hospitalization and neurodevelopmental delay at 18–24 months of corrected age in very low birth weight infants (VLBWIs), and to ascertain this association when adjusted for perinatal risk factors. Methods: This is a population-based study of VLBWIs born at 23–32 weeks of gestation between January 2014 and December 2017 who were enrolled in the Korean Neonatal Network. Bayley scales of infant development were evaluated at 18–24 months of corrected age in 2,098 infants. To test for LOS as a risk factor for neurodevelopmental delay, multiple logistic regression was used and adjusted for parental education status and clinical variables. Results: Blood culture positive LOS was identified in 419 (20.0%) infants. Cognitive and motor delays were found in 392 (18.7%) and 347 (16.5%) infants, respectively. When multivariate analysis was performed, LOS had a significant association with cognitive delay (odds ratio, 1.48; 95% confidence interval, 1.02–2.16), but no association with motor delay in VLBWIs. Both delays were significantly more frequent in cases of intraventricular hemorrhage (IVH) ≥ grade 3, periventricular leukomalacia (PVL), and intrauterine growth restriction (IUGR) and duration of mechanical ventilation. Male sex and necrotizing enterocolitis ≥ grade 2 had an effect on motor delay, whereas paternal college graduation affected cognitive delay. Conclusion: In VLBWIs with LOS, there is a heightened risk of cognitive delays at 18–24 months of corrected age. Brain injury, such as severe IVH and PVL, duration of mechanical ventilation, and IUGR, were also associated with cognitive and motor delays.

Experiences and efficacy of noninvasive prenatal test using maternal plasma in single center: 1,591 cases

So Yeon Hong,So Hyun Shim,Hee Jin Park,Sung Shin Shim,Ji Youn Kim,Yeon Kyung Cho,Soo Hyun Kim,Dong Hyun Cha 대한의학유전학회 2020 대한의학유전학회지 Vol.17 No.1

Purpose: The objective of this study was to analyze the results of several noninvasive prenatal tests (NIPTs) from a single cen-ter and conἀrm their efἀcacy and reliability. In addition, we aimed to conἀrm the changes in the number of invasive tests per-formed after introducing NIPT. Materials and Methods: NIPT data from a large single center from March 2014 to November 2018 were analyzed. Karyotyping was confirmed based on chorionic villus sampling, amniocentesis, or postnatal cord/peripheral blood sampling. Data on maternal age, gestational age, fetal fraction, and ultrasonographic results were analyzed. As the secondary outcome, the number of amniocentesis cases before and after the introduction of NIPT was compared. Results: Overall, 1,591 single pregnancy cases that underwent NIPT were enrolled. The mean maternal age was 36.05 (22-45) years. The average gestational age and fetal fraction were 12+1 (9+3 to 27+1) weeks and 10.95% (3.6% to 31.3%), respectively. A total of 1,544 cases (97.0%) were reported to have negative NIPT re-sults and 40 (2.5%) had positive NIPT results. The sensitivity and specificity of the overall abnormalities in NIPT were 96.29% and 99.36%, respectively. The positive predictive value (PPV) and negative predictive value were 72.22% and 99.93% respectively. The mean number of amniocentesis cases were 21.7 per month (21.7±3.9), which significantly decreased from 31.5 per month (31.5±4.8) before conducting NIPT as a screening test. Conclusion: NIPT is currently a useful, powerful, and safe screening test. In particular, trisomy 21 is highly speciἀc due to its high PPV. NIPT can reduce the potential risks of procedure-related miscarriages during invasive testing.

Maternal antimullerian hormone as a predictor of fetal aneuploidy occurring in an early pregnancy loss

( So Hyun Shim ),( Hyeong In Ha ),( Yong Wook Jung ),( Sung Shin Shim ),( Yeon Kyung Cho ),( Ji Youn Kim ),( Kyoung Jin Lee ),( Dong Hyun Cha ),( Soo Hyun Kim ),( Hee Jin Park ) 대한산부인과학회 2015 Obstetrics & Gynecology Science Vol.58 No.6

Objective The purpose of the study was to examine the relationship between the parameter representing ovarian reserve and the fetal aneuploidy in early spontaneous miscarriage. Methods A multicenter retrospective cohort study was performed in patients who were diagnosed with early pregnancy loss (≤13 gestational weeks) and examined for fetal karyotype at the CHA Gangnam Medical Center, CHA Bundang Medical Center, and CHA Gumi Medical Center between January 2011 and December 2012. Karyotyping was performed by the Genetic Laboratory of the Fertility Center of CHA Gangnam Medical Center. Medical records were reviewed for demographics, karyotype analysis and hormonal assay of ovarian reserve including antimullerian hormone (AMH) and follicle stimulating hormone. Statistical analysis was performed using SPSS software. Results A total 462 patients were included in this study. The mean age of the patients was 35.31±4.12 years and the mean AMH level was 3.88±3.50 ng/mL (n=195). Two hundred eleven conceptuses (45.7%) of patients showed the euploid and 251 (54.3%) showed the aneuploid. There are significant differences in maternal age, AMH and gestational age between fetal euploid and aneuploid groups (34.46±4.35 vs. 36.04±3.78 years, P<0.001; 4.60±3.86 vs. 3.43±3.18 ng/mL, P=0.022; 7.67±1.54 vs. 8.27±1.46 weeks, P<0.001, respectively). Multivariate analysis revealed that low AMH level and early gestational age were maternal age-independent markers for fetal aneuploid (P<0.001 and P=0.045, respectively). Conclusion Low maternal AMH level might be a predicting marker for fetal aneuploid in early pregnancy loss.

Experiences and Efficacy of non-invasive prenatal test (NIPT) using maternal plasma in single center: 1591 cases

( So Yeon Hong ),( So Hyun Shim ),( Soo Hyun Kim ),( Hee Jin Park ),( Sung Shin Shim ),( Ji Youn Kim ),( Yeon Kyung Cho ),( Kyung Jin Lee ),( Dong Hyun Cha ) 대한산부인과학회 2019 대한산부인과학회 학술대회 Vol.105 No.-

Objective: To analyze the result of a large number of NIPT in single center and confirm the efficacy and reliability. Methods: The data of NIPT in single center from Mar.2014 to Nov.2018 were analyzed. The patients who referred from other hospital due to abnormal result were also included. All of the patient have completed the delivery. Karyotyping confirmation was done by CVS or amniocentesis or postnatal cord blood sampling. We consider normal karyotype if there were no special abnormalities during pregnancy, no abnormal phenotype in neonate after birth. Details on age, gestational age, platform, fetal fraction, sonographic results were analyzed. Results: Total 1591 single pregnancy cases who performed NIPT were enrolled.640cases were individual SNP method, and 951cases were whole genome MPS method. Mean age was 36.1(22-45) years and gestational age, fetal fraction were 12+1(9+3-27+1)wks and 10.9(3.6-31.3)%.1544cases(97.1%) reported as negative NIPT results,40cases(2.5%) reported as positive NIPT results,7 cases(0.4%) were uninformed. In positive group, only 36cases cytogenetic confirmation tests were done whereas 4cases were F/U loss. Confirmed 36cases were following; Among the 19karyotype tests in NIPT trisomy21 positive group, all 19cases revealed as trisomy 21(100%).In 2cases of trisomy18 high risk group, 1case(50%) was confirmed as trisomy18.Trisomy13(n=1) was also confirmed as trisomy13.Among Sex chromosome abnormality group(n=8), 4cases showed discordancy result. Since we used MPS method, other uncommon trisomy;trisomy15(n=2),7(n=1) and 14(n=1) were incidentally detected. In this study, sensitivity and specificity were 96.3% and 99.4%, PPV and NPV were 72.2% and 99.9%,respectively. Conclusion: Clinical review of general cases are introduced based on single center’s results. NIPT is currently a useful, powerful and safe screening test, as the result we can reduce invasive test. In particular, trisomy 21 is highly effective due to its high PPV. In case of trisomy13,18 and SCA, confirmed test is necessarily required.

Increased Cutaneous Lymphocyte Antigen(CLA)+T Cells in the Peripheral Blood of Children with Severe Atopic Dermatitis

Shim, Jung-Yeon,Lee, So-Yeon,Kim, Hyo-Bin,Kim, Bong-Seong,Kim, Ja-Hyung,Seo, Ji-Hye,Jang, Seong-Ok,Jung, Seo-Hee,Hong, Soo-Jong 대한 소아알레르기 및 호흡기학회 2004 소아알레르기 및 호흡기학회지 Vol.14 No.2

Oxidative stress and the antioxidant enzyme system in the developing brain

Shim, So-Yeon,Kim, Han-Suk The Korean Pediatric Society 2013 Clinical and Experimental Pediatrics (CEP) Vol.56 No.3

Preterm infants are vulnerable to the oxidative stress due to the production of large amounts of free radicals, antioxidant system insufficiency, and immature oligodendroglial cells. Reactive oxygen species (ROS) play a pivotal role in the development of periventricular leukomalacia. The three most common ROS are superoxide ($O2^{\cdot-}$), hydroxyl radical ($OH^{\cdot}$), and hydrogen peroxide ($H_2O_2$). Under normal physiological conditions, a balance is maintained between the production of ROS and the capacity of the antioxidant enzyme system. However, if this balance breaks down, ROS can exert toxic effects. Superoxide dismutase, glutathione peroxidase, and catalase are considered the classical antioxidant enzymes. A recently discovered antioxidant enzyme family, peroxiredoxin (Prdx), is also an important scavenger of free radicals. Prdx1 expression is induced at birth, whereas Prdx2 is constitutively expressed, and Prdx6 expression is consistent with the classical antioxidant enzymes. Several antioxidant substances have been studied as potential therapeutic agents; however, further preclinical and clinical studies are required before allowing clinical application.

The Prediction of Bronchopulmonary Dysplasia in Very Low Birth Weight Infants through Clinical Indicators within 1 Hour of Delivery

Shim So-Yeon,Yun Ji Yun,Cho Su Jin,김민호,Park Eun Ae 대한의학회 2021 Journal of Korean medical science Vol.36 No.11

Background: Despite the advances in neonatology, the incidence of bronchopulmonary dysplasia (BPD) is increasing. It is important to prevent the development of BPD in the first place. The online BPD outcome estimator from National Institute of Children Health and Human Development and Neonatal Research Network is available. However, it is not applicable for Asians. Moreover, limits are set for birth weight and gestational weeks excluding those who may still have BPD. The aim of this study was to develop a prediction model for BPD using first hour perinatal and neonatal factors in Korean very low birth weight infants (VLBWIs). Methods: Data were collected for 8,022 VLBWIs with gestational age (GA) ≥ 22 weeks who were born between January 1, 2013 and December 31, 2016, and admitted to the neonatal intensive care units of the KNN. Multiple logistic regression models reanalyzed by stepwise selection with significant clinical indicators for BPD. PROC package was used to calculate the area under curve (AUC) and corresponding 95% confidence intervals. Moreover, it was used to search the best cut-off value. External validation was performed with the 2017 Korean neonatal network (KNN) data. Results: After all missing data were excluded, 4,600 VLBWIs were included in the training dataset of the prediction model. Predictability of presence of BPD was 90.8% and prediction P value cut off was 0.550. Five-minute Apgar score, birth weight, GA, sex, surfactant use were significant indicators. Predictability of severe BPD was 81.5% and prediction P value cut off was 0.160. Five-minute Apgar score, birth weight, maternal PIH, chronic maternal hypertension, GA, sex, respiratory distress syndrome, need of resuscitation at birth were significant indicators. After external validation, sensitivity and specificity did not change significantly. Conclusion: From this study, high predictability was obtained using clinical parameters obtained within one hour of life. P value for prediction of each grade of BPD and equation for calculation was presented. It can be helpful for the early prediction of BPD in Korean VLBWI. This study will contribute to the prediction of BPD in Asians especially Korean VLBWIs, not currently included in the NICHD BPD online BPD predictor. In addition, the predictive power may be continuously increased with the cumulative data of KNN.