A comparative study on the inhibitory effects of mast cell-mediated allergic reactions by artificially cultured and wild Acanthopanax senticosus

Yi, Jin-Mu,Jeong, Hyun-Ja,Shim, Kyung-Shik,Lee, Kang-Yong,Kim, Jeong-Sook,Zheng, Cui,Tomoko, Jippo,Lee, Young-Mi Kyung Hee Oriental Medicine Research Center 2000 International journal of oriental medicine Vol.1 No.2

We compared the effect between CAS and WAS(root, stem) on mast cell-mediated allergic reaction. CAS, WAS-root and WAS-stem, significantly inhibited compound 48/80-induced systemic allergic reaction(1g/kg) and histamine release from RPMC(1mg/ml). CAS, WAS-root and WAS-stem also inhibited passive cutaneous anaphylactic reaction. In addition, IgE-induced $TNF-{\alpha}$ secretion from RBL-2H3 was inhibited by pretreatment of CAS, WAS-root or WAS-stem$(0.01{\mu}g/ml)$. Taken together, inhibitory effect on mast cell-mediated allergic reactions of WAS-root is greater than those of WAS-stem but less than those of CAS.

Beneficial Effects of Cynaroside on Cisplatin-Induced Kidney Injury In Vitro and In Vivo

Nho, Jong-Hyun,Jung, Ho-Kyung,Lee, Mu-Jin,Jang, Ji-Hun,Sim, Mi-Ok,Jeong, Da-Eun,Cho, Hyun-Woo,Kim, Jong-Choon Korean Society of ToxicologyKorea Environmental Mu 2018 Toxicological Research Vol.34 No.2

Anti-cancer drugs such as cisplatin and doxorubicin are effectively used more than radiotherapy. Cisplatin is a chemotherapeutic drug, used for treatment of various forms of cancer. However, it has side effects such as ototoxicity and nephrotoxicity. Cisplatin-induced nephrotoxicity increases tubular damage and renal dysfunction. Consequently, we investigated the beneficial effect of cynaroside on cisplatin-induced kidney injury using HK-2 cell (human proximal tubule cell line) and an animal model. Results indicated that $10{\mu}M$ cynaroside diminished cisplatin-induced apoptosis, mitochondrial dysfunction and caspase-3 activation, cisplatin-induced upregulation of caspase-3/MST-1 pathway decreased by treatment of cynaroside in HK-2 cells. To confirm the effect of cynaroside on cisplatin-induced kidney injury in vivo, we used cisplatin exposure animal model (20 mg/kg, balb/c mice, i.p., once a day for 3 days). Renal dysfunction, tubular damage and neutrophilia induced by cisplatin injection were decreased by cynaroside (10 mg/kg, i.p., once a day for 3 days). Results indicated that cynaroside decreased cisplatin-induced kidney injury in vitro and in vivo, and it could be used for improving cisplatin-induced side effects. However, further experiments are required regarding toxicity by high dose cynaroside and caspase-3/MST-1-linked signal transduction in the animal model.

인동덩굴로부터 분리된 Cynaroside이 Doxorubicin으로 유도된 인간 근위세뇨관 HK-2 세포의 괴사에 미치는 저해 효과

노종현,정호경,이무진,장지훈,심미옥,정자균,정다은,안병관,조현우,Nho, Jong Hyun,Jung, Ho Kyung,Lee, Mu Jin,Jang, Ji Hun,Sim, Mi Ok,Jung, Ja Kyun,Jung, Da Eun,An, Byeong Kwan,Cho, Hyun Woo 한국약용작물학회 2017 한국약용작물학회지 Vol.25 No.5

Background: Cynaroside is a flavone, a flavonoid-like compound, known by different names (luteoloside and cinaroside). It is commonly found in Lonicera japonica Thunb., Chrysanthemum moriflium, and Angelica keiskei. The process of cell death has been classified as necrosis and apoptosis. Necrosis refers to unregulated cell death induced by a chemotherapeutic agent. Doxorubicin is an anthracycline anti-cancer drug used to treat acute leukemia, cancer, and lymphoma. However, it induces nephrotoxicity including tubular damage. Therefore, we investigated the protective effect of cynaroside against doxorubicin-induced necrosis in HK-2 cells. Methods and Results: To confirm the beneficial effect of cynaroside on doxorubicin-induced necrosis, HK-2 cells, a human proximal tubule epithelial cell line were treated with $10{\mu}M$ doxorubicin and $80{\mu}M$ cynaroside. Doxorubicin treatment resulted in increased DNA fragmentation, caspase-3 activity and mitochondria hyperactivation during cell necrosis. However, pretreatment with $80{\mu}M$ cynaroside attenuated DNA fragmentation, caspase-3 activity and mitochondria hyperactivation induced by $10{\mu}M$ doxorubicin in HK-2 cells. Conclusions: These results indicated that pretreatment with cynaroside ameliorated doxorubicin-induced necrosis in HK-2 cells. Therefore, cynaroside be used as a therapeutic agent for improving doxorubicin-induced nephrotoxicity. However, further studies are required to evaluated the toxicity of cynaroside treatment in animals and to determine its protective effect against doxorubicin-induced nephrotoxicity in an animal model.

사연 및 동판저수지의 식물성 플랑크톤에 관한 연구

강현무,박시섭,이상명,조현아,이진우,이호원 慶南大學校 附設 基礎科學硏究所 1995 硏究論文集 Vol.7 No.-

본 연구는 1994년 2,5,8,11월의 4회에 걸쳐 실시하였다. 조사 결과 동정 분류된 식물성 프랑크톤은 사연호가 6강 126분류군, 동판저수지가 5강 100분류군으로 전체 6강 211분류군이 출현하였다. 월별 종조성은 사연호에서 5월이 50분류군, 8월이 43분류군, 2월이 31분류군 순으로 출현하였다. 동판저수지는 5월이 49분류군, 11월이 27분류군, 2월 25분류군, 8월이 24분류군 순이다. 강별 구성비에서 두 지역모두 녹조강이 가장 많고 다음이 규조강 순이다. 전체 종조성은 사연호는 녹조강 54.3%, 규조강이 25.2%, 유글레나강 7.9%, 남조강 7.1%, 황녹조강 4.7%, 황갈조강 0.8%순이다. 동판저수지의 전출현 종에 대한 종조성은 규조강 51.4%, 녹조강33.9%, 유글레나강 11.0%, 황갈조강 3.7%, 황녹조강 10.9%의 종조성을 나타내었다. 환경요인은 pH의 경우 사연호 8월에 9.28로 가장 높고, DO는 동판 5월이 12.3㎎/ℓ으로 가장 높게 나타났다. 클로로필 a는 사연 2월이 13.1㎎/㎥로 가장 높으며, 부유물질은 동판저수지 5월에 85.0㎎/ℓ으로 가장 높았다. Phytoplankton flora of Sayoun and Tongpan-Reservoirs were studied. A total of 204 taxa (Sayoun-Reservoir, 126 taxa : Tongpan-Reservoir, 100 taxa) were indentified. The Major taxa of two localities was Chlorophyceae 46.7% and Bacillariophyceae 35.1%. As the bloom causing species. Microcystis aeruginosa. Ceratium hirudinella and Peridinium divergens were identified. The chlorophyll a concentration of Tongpan-Reservoir was higher then Sayoun-Reservoir.

플라이애시를 다량 사용한 콘크리트의 수화발열 특성과 압축강도 특성에 관한 실험적 연구

조규현,박무영,백민수,김우재,임남기,정상진 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.2

This study is for the great quantity use of fly-ash. For the producing of high volume concrete from the use of fly-ash, the method of replacement between bonding agents and fine aggregate by fly-ash at the slt was used that the adiabatic temperature rise of concrete about the mass member which bad been produced by the method that was mentioned before, and the hydration heat of the core test pieces in concrete was measured. Also the core test pieces which were replaced with fly-ash was studied by the compressive strength's comparison between standard care test pieces and core test pieces. In the case of mass test pieces, hydration heat and the time to reach the highest temperature were decreased by an increase in replaced fly-ash's amounts of concrete. In addition, among the test pieces having the same amounts of concrete, the test pieces having more replaced amounts of fly-ash's fine aggregate showed higher hydration heat and the increased time to reach the highest temperature. Compressive strength was also increased by hydration heat's decrease according to fly-ash replacement. Replacement of fly-ash was more effective in high temperature environment.

大學生의 思考技能의 診斷과 改善에 關한 硏究 : It's Diagnosis and Improvement

朴鎭玹,金武吉,金祥源,朴敬玉,羅東晋 全北大學校 學生生活硏究所 1988 學生生活硏究 Vol.16 No.-

Cognitive psychologists have taken a growing interest in reasoning skills as a primary goal of education. This study was accomplished as a part of elementary research on reasoning skills that have been pointed out as short comings of teaching of thinking. This study was composed of two substudies : one was to explore the ways to analyzing and diagnosing strategies for college students to apply to reasoning and problem solving, and the other was to examine the teachability of a normative strategy of thinking. In the first part of this study, we explored three alternatives to analyze thinking skills : cognitive process analysis, cognitive structure analysis, and cognitive style analysis ; and suggested the integrative use of them in diagnosing the cognitive skill of reasoning and problem solving. Model of cognitive process analysis was to devide the executive process of a specific task into several subtasks or components, measure the amount of effort, response time, and achievement level for each of them, and thereby analyze the strategies of thinking. Model of cognitive structure analysis was to present subject a specific task, instruct him to think aloud those ideas that came into his head during this solution, and analyze his cognitive structure on the base of his thinking-aloud. Finally, model of cognitive style analysis put more stress on tendency in thinking rather than ability in problem solving. In this regard, a alternative presented in this study was to analyze the strategies of thinking according to Baron's cognitive style model, in which process of thinking activity was devided into four phases : search of goal, search of possibility, search of evidence, and use of evidence. For each of them, the amount of time and effort that the indevidual invested and patterns of value that he grant would become criteria for judging the strategies that would become criteria for judging the strategies that he might use in the process of thinking activities for reasoning and problem solving. In result, this should correspond to measure biases on thinking. In general, main biases on thinking could be referred to insufficient search, search biased toward affirmative evidence, and oversensitivity to supporting evidence, which corresponded to compulsiveness vs. reflectiveness, dogmatism vs. open-mindedness, and authoritarianism, from a point of view on cognitive style, respectively. In the second part of this study, we examined the general principles of teaching thinking skills, based on human informations processing, and several training programs that have been developed with the purpose of improvement in thinking and reasoning skills. These programs were discussed into five categories: (1) cognitive operations approaches, (2) heuristics oriented approaches, (3) formal thinking approaches, (4) symbolic facility approaches, and (5) thinking-about-thinking approaches. Within these programs, the expansion of strategies and control of cognitive styles in reasoning and problem solving have become the main targets for improving of thinking skills. Therefore, this study was attempted to develop two training programs―referred to as Problem-Solving Training Program and Reasoning Skill Training Program respectively― for the purpose of improving these skills, and to test the effectiveness of these programs with college students. This study has shown the following facts : 1) It is necessary to teach the basic strategies for solving cognitive tasks of subject matters such as writing and mathematical problem for the purpose of improving thinking skills. 2) It is necessary to change the relatively general tendency in thinking process such as cognitive styles, in parallel with training of strategies. 3) Also, the training programs must consider indevidual defferences in activity parameters such as self-knowledge about problem solving, motivation, belief, etc. These facts seemed to imply the necessity of multi-dimensional approach to training the thinking skills.

급냉 제강슬래그를 잔골재로 사용한 차폐콘크리트의 공학적 특성에 관한 실험적 연구

강기웅,조성현,정원섭,권기주,김진만,김무한 대한건축학회 2004 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.24 No.1(구조계)

The steel slag, a by~product which is produced during the manufacture of steel by refining pig iron, is mainly used as road materials after aging it. It is necessary to age steel slag for long time in air because the reaction with water and free-CaO in steel slag could make the expansion of volume. This problem prevents steel slag from being used as aggregate for concrete. However, steel slag used in this study was controled by a air-jet method which rapidly cools substance melted at a high temperature. The rapid-chilling method prevents from generation of free-CaO in steel slag. The rapid-chilled steel slag is similar to be spherical shape and has the heavier specific gravity than natural aggregate. Therefore, it could be used to manufacture radiation shielding concrete as fine aggregate. This paper investigated the engineering properties of radiation shielding concrete when using rapid-chilled steel slag as fine aggregate. As results of this study, the increase of use of rapid-chilled steel slag decreased the desirable water content of concrete but developed the rate of radiation shielding of concrete. The strength of concrete using it is similar to that of concrete using river sand.

Enhanced Gas Sensing Properties of Pt-Loaded TeO₂ Nanorods

Jin, Chang-Hyun,Park, Sung-Hoon,Kim, Hyun-Su,Lee, Chong-Mu Korean Chemical Society 2012 Bulletin of the Korean Chemical Society Vol.33 No.6

The $NO_2$ gas sensing properties of multiple-networked, Pt-loaded $TeO_2$ nanorod sensors were examined. Scanning electron microscopy revealed nanowires with diameters of 50-100 nm and lengths of a few micrometers. Transmission electron microscopy and X-ray diffraction showed that the nanrods were tetragonal-structured, single crystal $TeO_2$. The Pt-loaded $TeO_2$ nanorod sensors exhibited sensitivities of 11.00, 10.26, 11.23 and 11.97% at $NO_2$ concentrations of 10, 50, 100 and 200 ppm, respectively, at $300^{\circ}C$. These sensitivities were more than 10 times higher than those of bare-$TeO_2$ nanorod sensors. The response times of the sensors were 310, 260, 270 and 230 sec at $NO_2$ concentrations of 10, 50, 100 and 200 ppm, respectively. The recovery times of the Pt-loaded $TeO_2$ nanorods were 390, 330, 335, and 330 sec at $NO_2$ concentrations of 10, 50, 100 and 200 ppm, respectively. The origin of the enhanced sensing properties of the $TeO_2$ nanorods by Pt loading is discussed.

Cedrol Enhances Extracellular Matrix Production in Dermal Fibroblasts in a MAPK-Dependent Manner

( Mu Hyun Jin ),( Sun Gyoo Park ),( Yul Lye Hwang ),( Min Ho Lee ),( Nam Ji Jeong ),( Seok Seon Roh ),( Young Lee ),( Chang Deok Kim ),( Jeung Hoon Lee ) 대한피부과학회 2012 Annals of Dermatology Vol.24 No.1

Background: The extracellular matrix (ECM) produced by dermal fibroblasts supports skin structure, and degradation and/or reduced production of ECM are the main causes of wrinkle formation. Objective: The aim of this study was to identify the active ingredient that enhances ECM production in dermal fibroblasts. Methods: Polarity-based fractionation was used to isolate the active ingredient from natural extracts, and the effects of cedrol (isolated from Pterocarpus indicusirginia) on ECM production in cultured human dermal fibroblasts was investigated by reverse transcription- polymerase chain reaction, enzyme linked immunosorbent assay, and Western blot analysis. Results: Cedrol accelerated fibroblast growth in a dose-dependent manner and increased the production of type 1 collagen and elastin. Phosphorylation of p42/44 extracellular signal-regulated kinase, p38 mitogen-activated protein kinase, and Akt was markedly increased by cedrol, indicating that enhanced ECM production is linked to activation of intracellular signaling cascades. Conclusion: These results indicate that cedrol stimulates ECM production, with possible applications to the maintenance of skin texture. (Ann Dermatol 24(1) 16∼21, 2012)

CO Gas-Sensor Based on Pt-Functionalized Mg-Doped ZnO Nanowires

Jin, Chang-Hyun,Park, Sung-Hoon,Kim, Hyun-Su,An, So-Yeon,Lee, Chong-Mu Korean Chemical Society 2012 Bulletin of the Korean Chemical Society Vol.33 No.6

Mg-doped ZnO one-dimensional (1D) nanostrutures were synthesized by using a thermal evaporation technique. The morphology, crystal structure, and sensing properties of the Mg-doped ZnO nanostructures functionalized with Pt to CO gas at $100^{\circ}C$ were examined. The diameters of the 1D nanostructures ranged from 80 to 120 nm and that the lengths were up to a few tens of micrometers. The gas sensors fabricated from multiple networked Mg-doped ZnO nanowires functionalized with Pt showed enhanced electrical response to CO gas. The responses of the nanowires were improved by approximately 70, 69, 111, and 81 times at CO concentrations of 10, 25, 50, and 100 ppm, respectively. Both the response and recovery times of the nanowire sensor for CO gas sensing were not nearly changed by Pt functionalization. It also appeared that the Mg doping concentration did not influence the sensing properties of ZnO nanowires as strongly as Pt-functionalization. In addition, the mechanism for the enhancement in the CO gas sensing properties of Mg-doped ZnO nanowires by Pt functionalization is discussed.