수유방법 및 기간이 아동의 타액내 환원효소활성검사성적과 유치우식경험도에 끼친 영향

배상만,노동주,김대업,이광희 大韓小兒齒科學會 1996 大韓小兒齒科學會誌 Vol.23 No.3

The purpose of this study was to investigate the effects of the lactation of human or bovine milk and the method of using nursing bottles on the salivary invertase test scores and deciduous caries experience of children. 328 preschool children (boys 178, girls 150) in Iksan city were selected for this study. The parents of the children were asked to answer the questionnaire about the lactation of human or bovine milk and the time of using nursing bottles. The data were obtained from the salvary invertase activity score,(Resazurin Disc Test) deciduous caries experience and the questionnaire. The result were as follows. The invertase activity and caries experience of the human milk higher than that of the bovine milk group(p<0.05). There were significant positive relationships between the lactation period and the invertase activity and between the lactation period and the caries experience(p<0.05). Caries experience had a tendency to increase as the time of using nursing bottles increase(p>0.05).

경기변동과 고소득층 소비지출에 대한 연구

배미경,박광희 啓明大學校 生活科學硏究所 2006 科學論集 Vol.32 No.-

This study investigated the consumption patterns of high-income households and analyzed the ratio of major 6 expenditures including food at home, food away from home, education, clothing, entertainment, and housing expenditure to total expenditure. The Daewoo Panel data was used and descriptive statistics, t-test and regression analysis were utilized for data analysis. Regression analyses were used to investigate the determinants of consumption pattern. To investigate effects of the economy depression, this study compared the consumption ratio for two different years. The results of study showed that among 6 categories, food away from home seemed to have larger effect of the economy depression but high-income household seemed not to lessen the education expense. The results showed that the age of reference person affected the amount of food expense, clothing and entertainment. This study would be useful to marketing strategies for fashion industries focusing on high-income households.

중합 광원과 중합 시간이 복합레진의 표면 경도에 미치는 영향

배상만,김대업,이광희,안호영 대한소아치과학회 2001 大韓小兒齒科學會誌 Vol.28 No.2

플라즈마 아크 광원을 사용하는 광중합기를 저출력 할로겐 광원을 사용하는 전통적인 광중합기와 비교 평가하기 위하여 세 종류의 복합레진을 두께가 2, 3, 4, 5mm인 몰드에 충전하고 레진 상면을 할로겐 광으로 40초간 플라즈마광으로 3, 6, 9초간 조사한 후 레진 상면과 하면의 표면미세경도를 각각 측정하였다. 레진시편 상면의 표면경도와 하면의 표면경도 간의 차이는, 두께 2mm 시편에 할로겐 광을 40초간 조사하였거나 플라즈마광을 9초간 조사한 경우들을 제외하고, 모두 유의하였다(P<0.05). 레진시편 상면의 표면경도는 전체 실험군들에서 서로 유의한 차이가 없었다. 레진시편 하면의 표면경도는 전체적으로 보아 할로겐 광을 40초간 조사한 군들에서 가장 높았고 플라즈마광의 조사시간이 감소함에 따라 감소하였으며 레진시편의 두께가 증가함에 따라 감소하였다. 이상의 결과는 복합레진의 중합깊이 측면에서 볼 때 3, 6, 9초간 조사하는 고출력 플라즈마광의 중합능력이 40초간 조사하는 저출력 할로겐광의 중합능력에 미치지 못함을 시사한다. The purpose of study was to compare the plasma are light with the halogen light in compostie resin curing. Three composite resin materials(Z-100. 3M, USA; Tetric Ceram. Vivadent, Liechtenstein; SureFil, Dentsply, USA) were filled in the teflon molds (4mm in diameter and 2,3,4,5mm in thickness) and cured with esther the conventional low-intensity light curing unit with a halogen lamp (Optilux 360, Demetron, U.S.A) for duration of 40 seconds or with the high-intensity light curing unit with a plasma are lamp (Flipo, Lokki, France) for duration of 3, 6, and 9 seconds. The intensity of halogen light was about 370mW/cm2 and that of plasma light was about 1,900mW/cm2. After one week, the surface hardnesses of both the top and the bottom of the resin samples were measured with a microhardness tester(MXT70, Matsuzawa, Japan). There ware significant differences in the hardness between the top and the bottom of the resin samples except the 2mm thickness samples cured by halogen light for 40s or by plasma light for 9s. There was no significant difference between the hardness values of the top surfaces of the thickness groups. The hardness values of the bottom surfaces decreased as the curing time decreased and as the thickness of resin samples increased, and the three kinds of resin composites showed similar patters. The results suggest that the halogen light for 40 seconds might be able to cure greater depth of resin composites than the plasma light far 3, 6, or 9 seconds.

전자근관장측정기 Root ZX의 정확도에 관한 실험적 연구

강대훈,정관희,윤수한,배광식 大韓齒科保存學會 1998 Restorative Dentistry & Endodontics Vol.23 No.1

The purpose of this study was to evaluate the in vitro accuracy of Root ZX(Torita Co., Japan) which is the ratio type electronic apex locator. The 86 extracted human palatal roots of maxillary molar with fully formed apices were used. File lengths with the file tip just visible at the foramen were compared to those measured with Root ZX. For length measuring with Root ZX, saline test model with which the apical 1/3 of each root was submerged into normal saline were designed. The root canal lengths were determined with Root ZX and the radiographs were taken with a file in the canal. The distances from file tips of Root ZX lengths to apecies in radiographs also were measured with Profile projector PJ311(Mitutoyo Co., Japan). The results were as follows : 1. The root canal length determined with electronic apex locator was 0.78±0.53 mm shorter than the length with visual measurement. 2. The file tip of Root ZX lengths was located at 0.85±0.49 mm away from the apex in radiograph. 3. The accuracy of the Root ZX was 79.1% within 0.5mm of visual working length and 96.5% within 1.0mm.

경연 대회를 통한 초ㆍ중고생의 창의력 신장 효과에 관한 연구

이희복,육근철,류해일,김현섭,김희수,박달원,유병환,김선효,김여상,서광수,변두원,서명석,배성효,박종석,심규철,이성희 공주대학교 과학교육연구소 2001 과학교육연구 Vol.32 No.1

미래 사회를 대비하기 위한 개인의 개성을 존중과 창의적인 능력을 지닌 인간을 양성을 목적으로 충청남도 지역의 초 ㆍ 중고등학생들을 대상으로 한 창의력 경연대회가 공주대학교 과학교육연구소에서 개최되었다. 충청남도 초 ㆍ중 ㆍ 고등학생들을 대상으로 실시한 창의력 경연대회는 창조적 아이디어를 내어 스스로 문제를 해결해 나갈 수 있는 교육 프로그램을 제공하는 좋은 기회였음이 확인되었으나, 일부의 팀에서만 참신하고 재미있는 아이디어들이 제안되었다. 여학생의 참가자 수가 상당히 증가하였으며, 대체적으로 입상권의 학생들의 상당 부분이 여학생이라는 것이 특이할만하다. 학년이 올라감에 따라서 창의적인 아이디어를 내는 양이나 질에 있어서 뒤떨어지는 것으로 분석되었으며, 토론 학습의 적응력이 미흡하고 발표력 및 청취력에 있어서도 떨어지는 것으로 나타났다. 따라서 중 ㆍ고등학교의 학교교육에서도 학생들에게 프로젝트형 탐구학습 프로그램의 적용은 물론 토론식 수습을 통한 학생들의 사고의 전환의 기회를 제공해야 할 것으로 사료된다. The creativity competition was held to foster the creativity of elementary and secondary school students in the Institute of Science Education, Kongju national University. It was found that the creativity competition effected on the improvement of inventing creative ideas and problem solving activities. But, only some competitors participated in semifinal and final contests, presented original and interesting ideas. More female students participated in competition than last year. More female participants were awarded a prize than males. Totally, participants were defective in discussion and communication, and presentation and listening ideas. Increasing grades correlated inversely with creativity and originality. There is need of inquiry project teaming programs and presenting opportunities of conversion of thinking by discussing instructions in school

GT rotary file을 이용한 만곡 근관형성시 작업 효율 및 file 변형 발생에 관한 연구

신주희,백승호,배광식,임성삼,윤수한,김병현 대한치과보존학회 2001 Restorative Dentistry & Endodontics Vol.26 No.5

Root canal preparation process is of utmost importance in successful treatment of root canal. Also, one of the most important purpose of the root canal preparation is to enlarge the root canal three dimensionally without changing the curvature of the root canal. However, as the curvature of the root canal increases, there are many difficulties involved in formation of optimum root canal. Therefore, in order to solve the above mentioned problems, new developments in methods of root canal preparation and equipments for such purposes were made. Recently, vigorous studies about newly introduced engine-driven nitckel-ti-tanium rotary file are conducted. As shown in research results to dates, it is well established that the use of nickel-titanium file is better suited for curved root canal than stainless steel file in maintaining the curvature of root canal and reducing the deformation of root canal. However, it is also acknowledged that there are a few discrepancies in research results according to protocol, due to failure to remove variables in experiments. In addition, although it is recommended by the manufacturer that the GT rotary file should maintain a low rotational speed of 150∼350rpm and 'light pressure' as light as not to break the lead of a pencil, academic studies about the vertical force which is not yet standardized are not sufficiently explored. Therefore, this research devised and utilized a special research equipment to standardize the appropriate range of vertical force for GT rotary file through experiments by breaking of the lead of a pencil as expressed by the manufacturer and to accurately measure factors involved through repeating and recreating the environment of root canal preparation. Forming nine experimental groups by varying the vertical forces (150g, 220g, 300g) and rpm (150rpm, 250rpm, 350rpm), the effects of changing vertical forces and rpm on working efficiency were measured in terms of time expended in root canal preparation by crown-down method using a transparent resin block with 35 degree curvature and GT rotary file (z-test). The following research using this special research equipment that involved nine experimental groups and varying the vertical force for root canal preparation from 300g which is within the normal vertical force range to 700g and 1000g which fall outside the normal rpm range. The results were as follows : 1.Analysis of the experiment results revealed that the tome spent in root canal preparation decreased as the vertical forces and rpm increased (p<0.05). Also, the effects of rpm were greater than those of the vertical forces within the normal vertical force range (β-weight test) . 2.Observation of the deformation of GT rotary file revealed that deformation increases in a direct correlation with the vertical force increase and in a reverse correlation with the rpm decrease, In the case of the vertical forces close to the normal range, the probability of GT rotary file deformation were quite different depending on the rpm changes. In the case of◎eater tier◎cal forces, the occurrences of deformation of the file were more frequent regardless of the rpm changes. 3.Deformation and breakage of file were also commonly observed in the expended time measurement experiments and GT rotary file deformation experiments in which low speed rpm (150rpm) was used and at the curved portion of the resin block. Key words : GT rotary file, Crown-down method, Working efficiency, Vertical force, Rpm, Deformation and breakage of file

다중 중합효소 연쇄반응을 이용한 소의 Johne병 진단 기법 확립

김종배,송혜원,김근희,김홍,신광순,김두 대한의생명과학회 2000 Journal of biomedical laboratory sciences Vol.6 No.1

반추수에서 발생하는 Johne병의 조기 진단 방법을 제시하고 이 질병의 원인체와 미생물학적 특징이 유사한 M. bovis, M. avium 등의 mycobacteria감염증을 감별 진단하는 방법을 개발하기 위하여 Mycobacterium 균속의 표준균주를 사용하여 중합효소 연쇄반응을 확립하였다. Johne병으로 의심되는 소의 혈액과 유즙을 채취하여 분리한 단핵구 및 거식세포로부터 genomic DNA를 추출하였다. 각 시료로부터 추출한 DNA를 template로 이용하여 Mycobacterium spp.에 특이적인 16S rDNA primer set를 이용한 PCR을 수행하여 시료내의 mycobacterial DNA 보유 여부를 확인하였다. 한편 mycobacteria 양성으로 확인된 시료는 M. avium complex 균종에 특이한 16S rDNA 염기서열을 기초로하여 제작한 primer set와 M. paratuberculosis 의 IS900 sequence에 특이한 primer set를 이용하여 duplex PCR을 수행하여 Johne병 원인체의 보균 여부를 조사하였으며, 이 결과를 oligonucleotide probe를 사용한 Southern blot hybridization을 통하여 다시 확인하였다. 이와 같은 duplex PCR 기법을 실제 축산 현장에서 수집한 유즙과 말초혈액으로부터 분리한 단핵구 및 거식세포 시료에 적용한 결과 본 연구에서 확립한 duplex PCR기법 유용성을 확인할 수 있었다. Diagnosis of Bovine Johne's Disease Using Multiplex Polymerase Chain Reactions In order to improve the early diagnosis of Johne's disease in ruminants, duplex polymerase chain reaction system for the detection of the etiologic agent of M. paratuberculosis and for the differentiation of other mycobacterial animal pathogens, such as M. bovis and M. avium, was applied. Genomic DNAs were purified from peripheral blood monocytes or milk macrophages and were used as templates in the duplex PCR. Detection of Mycobacterium spp. in the specimen was carried out by PCR using primer set specific to the mycobacterial 16S rDNA. And then, mycobacterial DNA-positive specimens were further differentiated with duplex PCR system which was composed of primer sets specific to 16S rDNA of M. avium complex and IS900 gene of M. paratuberculosis. The results were re-confirmed by Southern blot hybridization with oligonucleotide specific to the internal sequence of IS900 PCR amplicons. The applicability of this duplex PCR system was evaluated with DNAs extracted from clinical specimens of peripheral blood monocytes and milk macrophages. In summary, the duplex PCR amplification system described in this experiment is promising molecular technique for the early diagnosis of Johne's disease in ruminants.

레진 계열 근관봉함재 Adseal의 세포독성에 관한 연구

김희정,백승호,이우철,박한수,배광식 大韓齒科保存學會 2004 Restorative Dentistry & Endodontics Vol.29 No.6

The properties of ideal root canal sealers include the ability of sealing the total root canal system and no toxic effects to periradicular tissues. Cytotoxicity test using cell culture is a common screening method for evaluation of the biocompatibility of root canal sealers. The purpose of this study was to investigate the cytotoxic effect of newly developed resin-based sealer (Adseal 1, 2, and 3) comparing with those commercial resin-based sealers (AH26 and AH Plus), ZOE-based sealers (Tubliseal EWT, Pulp Canal Sealer EWT) and calcium hydroxide based sealer (Sealapex). An indirect contact test of cytotoxicity by agar diffusion was performed according to the international standard ISO 10993-5. L929 fibroblast cells were incubated at 37℃ in humidified 5% CO₂-containing air atmosphere. The freshly mixed test materials were inserted into glass rings of internal diameter 5 ㎜ and height 5 ㎜ placed on the agar. After the 24 hrs incubation period, the decolorization zones around the test materials were assessed using an inverted microscope with a calibrated screen. A Decolorization Index was determined for each specimen. Adseal 1, 2, and 3 did not exert any cytotoxic effects, whereas AH26, AH Plus, Tubliseal EWT, Pulp Canal Sealer EWT, and Sealapex produced mild cytotoxicity.

한국인에서 혈소판 당단백 Ⅲa 유전자 다형성과 급성 관동맥 증후군의 관계

김광일,채인호,김효수,손대원,오병희,이명묵,박영배,최윤식,이영우 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.5

심근침범을 동반한 Becker형 근이영양증 환자에서 확인한 Dystrophin 유전자 Exon결실

김광일,오병희,이무용,채인호,신수,박성섭,김효수,손대원,이명묵,박영배,최윤식,이영우 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.5