난치성 백반증에 대한 흡입물집을 이용한 비배양 표피세포이식술 20례

배정민 ( Jung Min Bae ),정한미 ( Han Mi Jung ),이한나 ( Han Na Lee ),이로우 ( Ro Woo Lee ),은성혜 ( Sung Hye Eun ),권혁선 ( Hyuck Sun Kwon ),이지혜 ( Ji Hae Lee ),김경문 ( Gyong Moon Kim ) 대한피부과학회 2018 대한피부과학회지 Vol.56 No.7

Background: As nonsurgical interventions for vitiligo are not always successful, various surgical modalities have been used in patients with refractory vitiligo. Of these, non-cultured epidermal suspension transplantation (NCES) was recently introduced to treat large recipient sites using cells from small donor tissue. Objective: We assessed the effectiveness and safety of NCES as a surgical treatment for patients with refractory vitiligo. Methods: We retrospectively reviewed 20 cases in 17 patients (11 females; median age 25 years) who underwent NCES from July 2015 through March 2018. Suction blisters (20 mm in diameter) were collected from the patient’s inner thigh at a donor-to-recipient area ratio of 1:5. After the addition of 5 mL recombinant trypsin solution to the suction blisters, followed by incubation at 37°C for 60 min, epidermal cells were manually scraped off the blister surface, and epidermal cell suspension was obtained by centrifugation at 1,500 RPM for 5 min. The suspension was applied to the vitiligo regions after epidermal ablation of those regions. Phototherapy resumed 1 month later. Treatment success was defined as ≥75% repigmentation of the surgical site, and all adverse events were noted. Results: Overall, 85.0% of cases (17/20) exhibited treatment success. Adverse events included hyperpigmentation (20%) and surgical site infection (5%), but the treatment was tolerable in all cases. Conclusion: NCES is a reliable surgical option for patients with vitiligo refractory to nonsurgical treatment. Large areas of vitiligo can be treated by NCES, and use of this technique should be encouraged in Korea. (Korean J Dermatol 2018;56(7):426∼432)

다문화가정 어머니의 언어 능력, 자발발화 특성과자녀의 자발발화 특성 분석

이정민 ( Jung Min Lee ),박혜원 ( Hye Won Park ),배성봉 ( Sung Bong Bae ) 경남대학교 인문과학연구소 2012 人文論叢 Vol.29 No.-

15분의 자유놀이 상황에서 수집한 40쌍의 다문화가정 어머니와 자녀의 자발발화 특성을 평균발화길이와 문법적 형태소 사용의 측면에서 분석하였다. 다문화가정 어머니의 문법적 형태소의 사용빈도는 낮았으며 MLU는 3.53(.84)이었다. 다문화가정 아동의 MLU는 2.39(.79)였으며 한국아동의 수준보다 낮았다. 아동과 어머니의 언어능력과 서술 격조사, 용언접미사, 선어말어미간 사용간에는 유의한 정적 상관이 있었다. Forty multicultural mothers` language skills, language characteristics and those of children`s during 15 min. of free play were analyzed in terms of grammatical morphemes and MLU. The use of grammatical morphemes was low in multicultural mothers and the MLU of multicultural mothers was 3.53(.84) The MLU of children with multicultural mothers was 2.39(.79) which is low compared with that of Korean children. Mothers` language abilities and use of particle endings, suffix for a declinable word, suffix before endings was positively correlated with those of children`s.

12주간의 태권도 트레이닝이 남자 청소년의 골밀도 및 골 건강관련 호르몬에 미치는 영향

김민형 ( Min Hyung Kim ),정현철 ( Hyun Chul Jung ),강효정 ( Hyo Jung Kang ),김현배 ( Hyun Bae Kim ),송종국 ( Jong Kook Song ) 한국스포츠정책과학원(구 한국스포츠개발원) 2012 체육과학연구 Vol.23 No.4

The purpose of the study was to evaluate the effects of weeks taekwondo training on bone mineral density, and calcemic hormones in male adolescents. 19 male adolescents aged 13-14 years (12 for training group; 7 for control group) were participated in this study. Taekwondo group has been performed 50 minutes a days a week for 12 weeks, as exercise intensity of 60-70 % HRR (heart rate reserver). Control group have only performed the activities included in their physical education classes. Skeletal maturity was assessed by TW3. Bone mineral density at the sites of lumber spine, forearm, femur and whole body were assessed by DXA (Hologic. QDR-4500, USA). Blood samples were drown in the morning after 12-h fast and spun at 3000 rpm for 15 min. 25(OH)D and calcitonin were analyzed by Liaison (Diasorin, USA). Osteocalcin was analyzed by COBAR 5010 (Quantum Packard. USA). Parathyroid hormone was analyzed by E170 (Roche, Germany) and growth hormone was analyzed by Immulite 2000 (DPC, USA). Statical analyses were done with SAS version 9.2. All data were presented in terms of means and standard deviations. Two-wany ANOVA with repeated measure was used for interaction of time x group. Paired t-test was perfomed to change over time in each parameter. Multiple regression analysis was used to find factors that influence dependent variables. A statistically significant level was defined as 95%. The results of this study indicates that bone age for TG (14.9±0.62yr) was significantly advanced compared to chronological age (14.0±0.64yr). but CG had similar bone age (14.1±0.55yr) compared to chronological age (13.9±0.46yr). significant within subjects effects were found for whole body BMD(p<.001), lumbar BMD (p<.001) and forearm BMD (p<.0001). Significant increase could be obtained in TG for whole body BMD, lumbar BMD, and forearm BMD between pre and post-tests However, there was no difference for femoral BMD for both TG and CG. Although no difference was found for serum osteocalcin in CG, significant increase could be observed in TG between pre and post-tests. There was significant within subject effect for PTH in TG (p<.05). Significant interaction effect of group and time was found for GH (p<.0001). serum 25 (OH)D was significantly decreased in both groups throughout the study periods. Bone age, serum calcitonin and lean tissue were predictors for whole body BDM in explaining 51.0%, 64% and 74.0% respective in TG. Bone age was significant predictor for lumbar BMD in explaining 39.0% in TG. In conclusion, 12 weeks taekwondo training has positive effects on bone mineral density and calcemic hormones in male adolescents. Further study is needed to enhance femral BMD as taekwondo training over 12 weeks and higher intensity. 이 연구의 목적은 12주간의 태권도 트레이닝이 남자 청소년의 골밀도와 골 건강관련 호르몬에 미치는 영향을 규명하는 것이었다. 연구대상자는 13~14세 남자 청소년 19명이었으며, 이들을 훈련군(12명)과 통제군(7명)으로 분류하였다. 골격성숙도는 TW3 방법을 적용하여 RUS 점수와 뼈나이를 평가하였고, 골밀도는 DXA를 이용하여 측정하였다. 골 건강관련 호르몬은 osteocalcin(OC), calcitonin(CC), parathyroid hormone(PTH), growth hormone(GH)과 25-hydroxyvitamin D(25(OH)D)를 분석하였다. 그룹 간 태권도 트레이닝 전·후 종속변인의 차이를 규명하기 위해 반복 이원분산분석을 적용하였고, 그룹 내 차이는 대응 t-검정을 이용하여 분석하였다. 골밀도와 뼈나이, 체중, 신장, 골 건강관련 호르몬의 관계를 규명하기 위해 다중회귀분석을 적용하였다. 이 연구 결과 훈련군의 전신 골밀도(p<.001), 요추 골밀도(p<.001), 전완 골밀도(p<.01)는 12주 태권도 트레이닝 전·후 통계적으로 유의하게 증가하였으나 통제군은 유의한 변화가 없었다. 한편 대퇴 골밀도는 훈련군과 통제군 모두 12주 트레이닝 전·후 유의한 변화가 나타나지 않았다. OC는 훈련군(pre: 13.18ng/mL, post: 40.38ng/mL)과 통제군(pre: 13.04ng/mL, post: 40.89ng/mL) 모두 통계적으로 유의하게 증가한 반면, CC는 두 그룹 모두 유의한 변화가 없었다. PTH는 훈련군의 경우 통계적으로 유의하게 감소하였지만(p<.05), 통제군은 유의한 차이를 보이지 않았다. GH는 트레이닝 전·후 훈련군과 통제군에서 유의한 상호작용효과가 나타났으며(p<.05), 훈련군(pre: 0.70ng/mL, post: 4.22ng/mL)에서는 증가한 반면 통제군(pre: 5.40ng/mL, post: 2.04ng/mL)에서는 감소하였다. 25(OH)D는 훈련군과 통제군 모두 통계적으로 유의하게 감소하였다. 골밀도의 예측변인을 분석한 결과 훈련군에서 전신 골밀도의 경우 뼈나이, 제지방조직, 신장이 높은 설명력을 보였고, 요추 골밀도의 경우 뼈나이, 전완 골밀도의 뼈나이, PTH, 생활나이가 유의한 설명력을 나타냈다. 결론적으로 12주간의 태권도 트레이닝은 남자 청소년들의 골밀도와 골 건강관련 호르몬에 긍정적인 영향을 미친 것으로 나타났으며 태권도 트레이닝이 청소년들의 요추 골밀도에 영향을 미치기 위해서는 12주 이상의 장기간의 훈련을 통한 후속연구가 필요할 것으로 사료된다.

초등학교 저학년용 영양교육 소책자 개발 및 효과 분석

손민정(Min Jung Son),조영숙(Young Sook Cho),김세나(Se Na Kim),서혜지(Hye Ji Seo),김숙배(Sook Bae Kim) 대한지역사회영양학회 2011 대한지역사회영양학회지 Vol.16 No.6

The purpose of this study was to investigate effects of nutrition education program and pamphlet for the lower grades elementary students focused on individual daily needed food exchange units using Food Exchange System. Program consisted of four lessons (40 min/lesson), 5 major nutrients & function, 6 food group and sources, daily needed food exchange units for normal body weight, and smart snack choice and exercise. Pamphlet as activity book was developed for the program. The subjects were 3rd grade elementary students (educated group, 31 vs. non-educated group, 31). Educated group were lessoned as group and/or individual. We examined the differences in nutrition knowledge, dietary attitudes, dietary intakes and satisfaction of the program and pamphlet. In educated group, there were positive improvements on nutrition knowledge score function and foods of 5 nutrients and on dietary attitudes type of breakfast and snacks. In the evaluation of dietary intakes according to KDRI, there were positive improvements on intakes level of riboflavin, vit. C, folate, Ca, P, Fe and Zn in educated group. In satisfaction with the program and pamphlet, contents, font size, visual, figure, difficulty and program curriculum were over 2.90/3.0. It showed that the developed nutrition education program and pamphlet focused on individual daily needed food exchange units using Food Exchange System improved nutrition knowledge, dietary attitudes and nutrients intake level in the lower grades elementary students. (Korean J Community Nutr 16(6) : 647~660, 2011)

H9c2 심근 세포주에서 외인성 nitric oxide가 허혈에 의한 세포 독성에 미치는 영향

정성구,장현용,김명천,고영관,정주호,배영미,박원서,김대중,유영민,김성수,임성빈 대한응급의학회 2001 대한응급의학회지 Vol.12 No.4

Background: Nitric oxide(NO) is known to have protective effects on an ischemic heart and to exert triggering effects on ischemic preconditioning. However, the effects of NO during the ischemic period have not been investigated. To investigate the role of exogenous nitric oxide in a model of ischemic heart cell death, we studied the effects of ischemic preconditioning and ischemia in a normal and an ischemic buffer. Methods: Rat cardiac myoblast cells(H9c2) were cultured in a normal and an ischemic buffered medium. For the ischemic culture of heart cells, the cells were cultured in a dessicator with GasPak for 5 hrs. In ischemic preconditioning, the cells were pretreated with ischemic buffer for 5 min and then perfused with normal medium for 30 min. For the measurement of the cytotoxicity, a MTT(3-4-Sdimethylthiazol-2-yl-2,5-diphenyl tetrazolium bromide) assay was performed. A DAPI(4',6-diamidino-2-phenylindole dihydrochloride) staining procedure and a flow cytometry analysis were performed to confirm apoptotic cell death by ischemia. Results: Cell viability, as determined by using a MTT assay, showed that the preconditioned group treated with NO showed more cell death than with the not-preconditioned groups in both normal and ischemic buffers. But, In normal medium and not-preconditioned groups, NO showed protective effect according to the concentrations(100,1000μM) . No treatment with NO produced the different results. In normal medium, the protective effect of ischemic preconditioning was demonstrated, but no protective effect of ischemic preconditioning could be seen in the case of the ischemic buffer. The DAPI staining and flow cytometry analysis of heart cells showed characteristic apoptotic features. Conclusion: NO added in the ischemic phase had deterious effects on heart cells. Ischemic preconditioning was more harmful than ischemia alone. The toxicity of the cells was characteristic apoptosis.

지방산 유지 원료에 따른 고형비누의 특성에 관한 연구

정명선,배한수,이봉연,민경혜,류덕환 계명대학교 생활과학연구소 2001 科學論集 Vol.27 No.-

The experimental results on the washing efficiency of detergent made from corn oil, wasted cooking oil and pig fat oil are as follows ; Laundry factors in this experiment are detergent concentration, laundry temperature and laundry time. 1.The surface tension of each detergent showed the lowest in 0.2% concentration corn oil 60.84, wasted cooking oil 61.11, pig fat oil 60.73dyne/cm. 2.The wasthing efficiency based on different concentration of detergents showed the highest in 0.2% concentration. 3.The highest washing efficiency appeared in 0.2% concentration 40℃ temperature and 40~50 min. time. There showed no difference in the washing efficiency according to the volume of water. It can be said that the frictional force of bearing rather than the volume of water worked on the efficiency.

온라인 쇼핑몰의 상품추천 시스템의 설계 및 실험 : 상품의 최신성을 고려한 사례기반추론 접근

배정연,김민용,권오병 경희대학교 국제경영연구소 2006 국제경영연구 Vol.12 No.1

Helicobacter pyloir 양성 소화성 궤양에서 lansoprazole 포함 삼제요법의 제균율 및 제균판정에 있어서 요소호기검사의 유용성

정혜경,곽재진,유민아,배기선,권정미,이종수,김도영,문일환 대한감염학회 2003 감염과 화학요법 Vol.35 No.3

목적 : 본 연구는 Helicobacter pylori (이하 H. polyri) 양성인 소화성 궤양 환자에서 lansoprazole 포함 삼제요법의 제균율을 알아보고, 제균 치료 후 제균 판정에 있어서 요소 호기 검사(Urea breath test, 이하 UBT) 및 신속요소분해(rapid urease test, 이하 RUT test)와의 일치율에 대하여 알아보고자 하였다. 방법 : 상부위장관내시경 검사에서 H. polyri 양성인 소화성 궤양환자에서 lansoprazole 60㎎+amoxicillin 2g+clarithromycin 1g의 약제를 2회 분복하여 1주간 복용하는 삼제요법을 실시하였다. 최소 4주후 추적 상부위장관내시경을 실시하였고, 전정부와 체부에서 각각 RUT를 실시하였으며 UBT(5분, 20분)를 시행하였다. 결과 : 대상환자는 총 46명으로 남자 34명(48±13세), 여자 12명(53±14세)이었다. LAC 삼제요법의 제균율은 40/46명(87.0%)이었고, 궤양의 치유율도 42/46명(91.3%)이었다. RUT와 20분 UBT 결과가 모두 음성이었던 경우는 41예, 모두 양성이었던 경우는 4예로 RUT와 UBT는 97.8% (45/46)의 일치율을 보였고, 1예는 UBT는 음성이었으나 체부에서 시행한 RUT가 양성이었다. 5분 UBT 검사가 양성이었던 예는 14/34 (41.2%) 이었고, 이 14예 중 12예(85.7%)는 20분 UBT 검사 음성, RUT 음성이었다. 결론 : Lansoprazole과 amoxicillin, clarithromycin 1주일 병합요법은 H. polyri 양성인 소화성 궤양 환자에서 87.0%의 제균율을 나타내어 우수한 효과를 보였고, 요소호기검사는 제균 치료 후 제균 판정에 유용한 비침습적인 방법임을 확인할 수 있었다. Background : There are only a few studies on Helicobacter pylori (H. pylori) for its eradication rates of lansoprazole-based triple therapy in Korea, and the results are controversial. Therefore, we undertook to investigate the eradication rate of lansoprazole-based triple therapy, and compare the concordance rate of urea breath test (UBT) and rapid urease test (RUT) in evaluating H. pylori eradication. Methods : Patients with acute peptic ulcer who were H. pylori-positive were recruited by prospective, consecutive manner. They received lansoprazole 30 ㎎ b.d., amoxicillin 1 g b.d. and clarithromycin 500 ㎎ b.d. for 1 week. Upper endoscopy was performed after 4 weeks to check for ulcer healing, and UBT and RUT were performed to evaluate H. pylori eradication status. Results : A total of 46 patients were recruited, and they were all compliant. H. pylori eradication rate was 87.0% (40/46) and ulcer healing rate was 91.3% (42/46). Forty one patients showed negative in both UBT and RUT, and 4 patients revealed positive in both tests, therefore, the concordance rate of UBT and RUT was 97.8% (45/46). Conclusion : Our study showed that 1-week lansoprazole-based triple therapy was effective in H. pylori eradication and ulcer healing. UBT can be an effective, noninvasive method for evaluating H. pylori status after H. pylori eradication.

레이저 2선 굴절법을 이용한 정적연소실에서 예혼합기의 연소속도 측정

배충식,김정민 忠南大學校 産業技術硏究所 1997 산업기술연구논문집 Vol.12 No.2

Burning velocities of propane-air mixtures were experimentally investigated in a constant-volume combustion chamber. The propane-air mixture was prepared by controlling partial pressures. Flame propagation process was observed as a function of mixture strength. A spherical combustion chamber contains a pair of parallel windows through which optical access into the chamber can be provided. Laser two beam deflection method was adopted to measure the local flame propagation, which gave information on the flame size and flame propagation speed. Pressure development was also measured by a piezoelectric pressure transducer to characterize combustion in quiescent mixtures. Burning velocity was calculated from flame propagation speed and pressure measurements. In this experiment, the values of flame speed are within totally 1.1% error range including 0.5% location error and 1% visual error. Laser two beam method was found to be feasible in measuring flame propagation in that the measured values are comparable to the available data in literatures.

온라인 쇼핑몰의 상품추천 시스템의 설계 및 실험 : 상품의 최신성을 고려한 사례기반추론 접근

배정연,김민용,권오병 경희대학교 기업경영연구소 2006 기업경영연구 Vol.12 No.1

고객의 구매를 직접적으로 유도하는 판매원이 존재하지 않는 온라인 쇼핑몰에서는 고객 스스로가 상품에 데한 정보를 획득-분석하여 구매의사결정을 해야 하는 한계점이 상존한다. 이를 해결하기 개발된 것이 온라인 쇼핑몰의 상품추천 시스템(Product Recommendations System)인데, 이 시스템은 웹상에서 획득한 고객정보와 고객구매이력 정보를 바탕으로 동일고객 혹은 유사 고객에게 적절한 상품을 추천하는 것이다. 이런 상품추친 시스템을 구현하기 위한 추천기법으로 사례기반추론이 가장 놀리 사용되어 왔다. 그런데, 기존 연구는 문제와 사례간의 특성이 ‘얼마나 유사한가?’에 초점을 두었기 때문에 베스트셀러에 해당하는 상품만이 계속 추친될 가능성이 높았다. 이런 단점을 보완하고 추천 상품의 다양성 및 마케팅 관점을 반영하기 위해 본 연구에서는 유사성과 합께 상품의 최신성을 동시에 고려하는 CBR-PR(Product Recency) 기반의 상품추천 시스템을 설계하고 그 성능을 실제 온라인 쇼핑몰의 데이터를 이용하여 실험하였다. 실험결과 본 연구에서 제안하는 CBR-PR 기법이 기존 기법에 비해 추천 적중률이 모두 높았다. Product recommendation systems have been widely advocated as a way of coping information overload for potential customers in purchasing products at the online shopping mall. To enhance the accuracy of product recommendations, a wide variety of techniques has been developed. Among these, CBR(Case-basyd Reasoning) is one of the most frequently used techniques. However, traditional CBE technique mainly focuses the similarity between current problem and past cases and hence it tends to by biased toward best selling products recommendation. To overcome this limitation and incorporate marketer's view, we designed CBR-PR(Product Recency) technique which considered not only product similarity but also the product rycency. In our paper rycyncy is measured not by the production date but by the launch date for the new product. Using real-world data, we also conducted the several experiments to compare performances of our approach with that of the traditional CBR. Experimental results showed that CBR-PR scored the better recommendation accuracy ratio than traditional CBR in every experimental settings.