X-선 회절법에 의한 소결광내의 칼슘페라이트 생성에 미치는 Al_2O_3, SiO_2 및 MgO의 영향

朴埈賢,趙兪貞,尹晟燮,許完旭,金亨順 대한금속재료학회 2002 대한금속·재료학회지 Vol.40 No.7

In order to elucidate the fundamental aspects of the minerals formation processes during sintering, the Fe_2O_3-CaO-Al_2O_3-SiO_2 system and the effect of MgO on synthesized iron ore sinter were studied by image analysis and X-ray diffraction method. Most existed calcium ferrite was identified to be a solid solution of CaO·2Fe_2O_3 with a small amount of solubility of Al_2O_3 and SiO_2. With an increase of the content of Al_2O_3 in the system, the amount of calcium ferrite was increased. On the other hand, the amount of calcium ferrite was decreased, and the amount of hematite was increased with increasing the content of SiO_2. Crystal structure of calcium ferrite with Al_2O_3 and SiO_2 was changed from monoclinic to triclinic as well as the unit cell volume shrank with fixing Al_2O_3 and increasing SiO_2. With increasing the content of MgO(1∼3 wt%), the amount of magnetite was increased and hematite was decreased but calcium ferrite was not changed.

슬링(sling)과 고정된 지지면에서의 팔굽혀펴기 동작 시 근 활성도 비교

오재섭,박준상,김선엽,권오윤 한국전문물리치료학회 2003 한국전문물리치료학회지 Vol.10 No.3

말기신부전 환자의 혈색소 순환 : 투석 방식에 따른 양상 비교 및 관련 인자들에 대한 고찰

최재혁,김준섭,원기범,박준형,탁우택,이정호 동국대학교 의학연구소 2009 東國醫學 Vol.15 No.2

혈색소 순환은 말기신부전 환자에서 인체 재조합 적혈구 조혈 인자를 투여하여 빈혈을 치료하는 동안 빈번히 관찰되며 이는 환자의 입원과 사망률과 관계가 있다고 알려져 있다. 이에 저자는 투석중인 말기 신부전 환자를 대상으로 혈색소 순환 양상과 이와 연관된 인자들을 알아보고자 하였다. 혈색소 순환의 관련 인자들을 알아보기 위하여 투석 치료를 받고 있는 환자 40명 (혈액투석 환자군: 20형, 복막투석 환자군: 20명)을 대상으로 투석 시작부터 1년 동안의 환자의 나이와 성별, 투석시작 1년간의 평균 Hb, 수혈 횟수, 총입원 횟수, 감염으로 인한 입원 횟수, 혈청 ferritin 농도, 혈청 transferrin saturation (TSAT), 철분제제 복용 유무, 일주일 동안 투여된 rHuEPO 양, rHuEPO의 변화량, 알부민, C-reactive protein (CRP) 및 입원 원인 등을 후향적으로 조사하였다. 전체 환자 40명 중 33명 (82.5%)에서 혈색소 순환이 관찰되었다. 혈액투석 환자군에 비해 복막투석 환자군에서 혈색소 순환 첫수가 유의하게 높았다 (p=0.037).혈색소 순환 발생군과 혈색소 순환 미발생군의 관련인자들을 비교하였을 경우 혈색소 순환 발생군에서 총 입원 첫수 (p=0.01), 감염으로 인한 입원 횟수 (p=0.036)가 유의하게 많았으며, 혈중 알부민 수치는 유의하게 낮았다 (p=0.003).그러나 두 군간의 나이, 성별, 수혈 횟수, 혈청 ferritin농도, 혈청 transferrin saturation (TSAT) 및 혈청 철분 농도는 유의한 차이가 없었다. 혈색소 순환의 빈도는 일주일 동안 투여된 r-HuEPO변화량과 양의 상관관계를 보였고 (p=0.003),혈중 알부민 수치와 음의 상관관계를 보였다 (p=0.013). 복막투석 환자군에서 혈액투석 환자군에 비해 혈색소 순환이 유의하게 높게 발생하였다. 일주일 동안 투여된 r-HuEPO 변화량, 입원 및 혈청 알부민 수치는 투석 시작부터 1년 동안의 혈색소 순환과 관련이 있었다. Hemoglobin cycling is frequently observed during treatment of anemia with recombinant human erythropoietin (r-HuEPO) in patients with end stage renal disease (ESRD) it is also reported to be associated with hospitalization and morbidity. We analyzed hemoglobin cycling and potential associated factors in patients on dialysis. Forty new dialysis patients (20 hemodialysis, HD and 20 peritoneal dialysis, PD) were recruited during their first year. Age, gender, mean hemoglobin level, frequency of transfusion, frequency of hospital admission, frequency of admission due to infection, serum ferritin, transferrine saturation, iron levels, rHuEPO dose per week, serum albumin, and c-reactive protein were retrospectively evaluated in order to examine the possible correlations with hemoglobin cycling. Thirty-three patients (82.5%) were identified in the hemoglobin cycling group. The PD group had a higher frequency of hemoglobin cycling than did the HD group (p=0.037). The hemoglobin cycling group showed a significantly higher frequency of hospital admission (P=0.001), admission due to infection (p=0.036) and a lower serum albumin level (p=0.003) than the other group, but there was no significant difference between the groups for age, gender, frequency of transfusion, serum ferritin, transferrine saturation, and iron levels. The frequency of hemoglobin cycling showed a positive correlation with the i-HuEPO dose variation per week (p=0.003) and a negative correlation with serum albumin level (p=0.013). The PD group had a higher frequency of hemoglobin cycling than the HD group, and the change of rHuEPO dose per week, hospitalization and serum albumin were associated with the hemoglobin cycling during the first year of dialysis.

The Effect of LidocaineㆍHCl on the Fluidity of Native and Model Membrane Lipid Bilayers

Jun-Seop Park,Tae-Sang Jung,Yang-Ho Noh,Woo-Sung Kim,Won-Ick Park,Young-Soo Kim,정인교,손의동,배수경,배문경,장혜옥,Il Yun 대한약리학회 2012 The Korean Journal of Physiology & Pharmacology Vol.16 No.6

The purpose of this study is to investigated the mechanism of pharmacological action of local anesthetic and provide the basic information about the development of new effective local anesthetics. Fluorescent probe techniques were used to evaluate the effect of lidocaineㆍHCl on the physical properties (transbilayer asymmetric lateral and rotational mobility, annular lipid fluidity and protein distribution) of synaptosomal plasma membrane vesicles (SPMV) isolated from bovine cerebral cortex, and liposomes of total lipids (SPMVTL) and phospholipids (SPMVPL) extracted from the SPMV. An experimental procedure was used based on selective quenching of 1,3-di(1-pyrenyl)propane (Py-3-Py) and 1,6-diphenyl-1,3,5-hexatriene (DPH) by trinitrophenyl groups, and radiationless energy transfer from the tryptophans of membrane proteins to Py-3-Py. LidocaineㆍHCl increased the bulk lateral and rotational mobility of neuronal and model membrane lipid bilayes, and had a greater fluidizing effect on the inner monolayer than the outer monolayer. LidocaineㆍHCl increased annular lipid fluidity in SPMV lipid bilayers. It also caused membrane proteins to cluster. The most important finding of this study is that there is far greater increase in annular lipid fluidity than that in lateral and rotational mobilities by lidocaineㆍHCl. LidocaineㆍHCl alters the stereo or dynamics of the proteins in the lipid bilayers by combining with lipids, especially with the annular lipids. In conclusion, the present data suggest that lidocaine, in addition to its direct interaction with proteins, concurrently interacts with membrane lipids, fluidizing the membrane, and thus inducing conformational changes of proteins known to be intimately associated with membrane lipid.

Overview of type 1 diabetes mellitus and its experimental models

Jun-Seop Shin,Byoung-Hoon Min,Su-Keong Park,Ji-Won Choi,Sunyoung Kim,Jong-Min Kim,Chung-Gyu Park 한국실험동물학회 2016 한국실험동물학회 학술발표대회 논문집 Vol.2016 No.2

Statistically Designed Enzymatic Hydrolysis for Optimized Production of Icariside 2 as a Novel Melanogenesis Inhibitor

( Jun Seong Park ),( Hye Yoon Park ),( Ho Sik Rho ),( Soo Mi Ahn ),( Duck Hee Kim ),( Ih Seop Chang ) 한국미생물 · 생명공학회 2008 Journal of microbiology and biotechnology Vol.18 No.1

Three kinds of prenylated flavonols, icariside I, icariside II, and icaritin, were isolated from an icariin hydrolysate and their effects on melanogenesis evaluated based on mushroom tyrosinase inhibition and quantifying the melanin contents in melanocytes. Although none of the compounds had an effect on tyrosinase activity, icariside II and icaritin both effectively inhibited the melanin contents with an IC50 of 10.53 and 11.13 μM, respectively. Whereas icariside II was obtained from a reaction with β-glucosidase and cellulase, the icariin was not completely converted into icariside II. Thus, for the high-purity production of icariside II, the reaction was optimized using the response surface methodology, where an enzyme concentration of 5.0 mg/ml, pH 7, 37.5℃, and 8 h reaction time were selected as the central conditions for the central composite design (CCD) for the enzymatic hydrolysis of icariin into icariside II using cellulase. Empirical models were developed to describe the relationships between the operating factors and the response (icariside II yield). A statistical analysis indicated that all four factors had a significant effect (p<0.01) on the icariside II production. The coefficient of determination (R2) was good for the model (0.9853), and the optimum production conditions for icariside II was an enzyme concentration of 7.5 mg/ml, pH 5, 50℃, and 12 h reaction time. A good agreement between the predicted and experimental data under the designed optimal conditions confirmed the usefulness of the model. A laboratory pilot scale was also successful.

Laparoscopic vs Transumbilical Single-Port Laparoscopic Appendectomy

Jun Ho Park,Kee Hoon Hyun,Chan Heun Park,Su Yun Choi,Won Hyuk Choi,Doo Jin Kim,Samuel Lee,Joo Seop Kim 대한외과학회 2010 Annals of Surgical Treatment and Research(ASRT) Vol.78 No.4

Purpose: Conventional three-port laparoscopic appendectomy (LA) is more commonly performed than transumbilical single port laparoscopic appendectomy (TUSPLA). In this report, we performed a prospective randomized study comparing the outcomes of LA and TUSPLA. Methods: Between April 14, 2009 and June 10, 2009, 40 patients who required laparoscopic appendectomies were randomly selected to receive either a TUSPLA or a LA. None of these patients had perforation or abscess. Twenty of the patients received a LA and the other 20 received a TUSPLA. The clinical outcomes and visual analog pain scores (VAS) were compared between the groups. Results: The TUSPLA procedures were performed successfully in every indicated patient. Clinical outcomes were similar in both study groups. The TUSPLA group showed a significantly higher VAS score 24 hours postoperatively than the LA group. Conclusion: Compared with LA, TUSPLA was technically feasible and safe in patients with non-complicated appendicitis. However, the patients in the TUSPLA group reported more postoperative pain than those in the LA group.

The Role of Nitric Oxide in the Expression of Renal Aquaporin 2 in a Cirrhotic Rat Model: Does an AVP-Independent Mechanism Exist for the Regulation of AQP2 Expression?

Jun, Dae Won,Park, Jin Hee,Park, Yoo Sin,Kang, Ju-Seop,Kim, Eun Kyung,Kim, Kyung Tae,Son, Byoung Kwan,Kim, Seong Hwan,Jo, Yun Ju,Park, Young Sook Springer-Verlag 2010 Digestive diseases and sciences Vol.55 No.5

Effects of Dimyristoylphosphatidylethanol and Ethanol on Thickness of Neuronal Membrane Lipid Bilayers

Jun-Seop Park,장혜옥,Eun-Jong Choi,Dong-Soo Jeong,Hak-Jin Yang,Kyoung-Phil Kim,Kuk-Kyung Son,Hyun-Wook Lee,Min-Jung Kang,Sang-Min Kim,정인교,배문경,Il Yun 대한약학회 2009 Archives of Pharmacal Research Vol.32 No.10

The aim of this study was to provide a basis for examining the molecular mechanism for the pharmacological action of ethanol. Energy transfer between the surface fluorescent probe 1-anilinonaphthalene-8-sulfonic acid and hydrophobic fluorescent probe 1,3-di(1-pyrenyl)propane was used to examine the effect of both dimyristoylphosphatidylethanol (DMPEt) and ethanol on the thickness (D) of the synaptosomal plasma membrane vesicles (SPMV) isolated from the bovine cerebral cortex. The thickness (D) of the intact SPMV was 1.044 ± 0.008 (arbitrary units, n=5) at 37oC (pH 7.4). Both DMPEt and ethanol decreased the thickness of the SPMV lipid bilayer in a dose-dependent manner with a significant decrease in thickness observed at 5 μM and 25 mM, respectively. It was assumed that both ethanol and DMPEt cause interdigitation in the SPMV lipid bilayers. The effects of ethanol on the neuronal membranes were attributed to its direct and indirect actions. The indirect action of ethanol refers to the action of phosphatidylethanol, which is an ethanol abnormal metabolite, on the neuronal membranes. The decrease in membrane thickness by both DMPEt and ethanol might be responsible for some, but not all of its anesthetic actions.

The Effect of Lidocaine.HCl on the Fluidity of Native and Model Membrane Lipid Bilayers

Park, Jun-Seop,Jung, Tae-Sang,Noh, Yang-Ho,Kim, Woo-Sung,Park, Won-Ick,Kim, Young-Soo,Chung, In-Kyo,Sohn, Uy Dong,Bae, Soo-Kyung,Bae, Moon-Kyoung,Jang, Hye-Ock,Yun, Il The Korean Society of Pharmacology 2012 The Korean Journal of Physiology & Pharmacology Vol.16 No.6

The purpose of this study is to investigated the mechanism of pharmacological action of local anesthetic and provide the basic information about the development of new effective local anesthetics. Fluorescent probe techniques were used to evaluate the effect of lidocaine HCl on the physical properties (transbilayer asymmetric lateral and rotational mobility, annular lipid fluidity and protein distribution) of synaptosomal plasma membrane vesicles (SPMV) isolated from bovine cerebral cortex, and liposomes of total lipids (SPMVTL) and phospholipids (SPMVPL) extracted from the SPMV. An experimental procedure was used based on selective quenching of 1,3-di(1-pyrenyl)propane (Py-3-Py) and 1,6-diphenyl-1,3,5-hexatriene (DPH) by trinitrophenyl groups, and radiationless energy transfer from the tryptophans of membrane proteins to Py-3-Py. Lidocaine HCl increased the bulk lateral and rotational mobility of neuronal and model membrane lipid bilayes, and had a greater fluidizing effect on the inner monolayer than the outer monolayer. Lidocaine HCl increased annular lipid fluidity in SPMV lipid bilayers. It also caused membrane proteins to cluster. The most important finding of this study is that there is far greater increase in annular lipid fluidity than that in lateral and rotational mobilities by lidocaine HCl. Lidocaine HCl alters the stereo or dynamics of the proteins in the lipid bilayers by combining with lipids, especially with the annular lipids. In conclusion, the present data suggest that lidocaine, in addition to its direct interaction with proteins, concurrently interacts with membrane lipids, fluidizing the membrane, and thus inducing conformational changes of proteins known to be intimately associated with membrane lipid.