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인삼 사포닌의 생화학적 연구 ( ⅩⅩⅤ ) : 인삼 사포닌 분획이 대두 발아시 Ascorbic acid 생합성에 미치는 영향
배수경,곽한식,주충노 ( Soo Kyung Bae,Hahn Shik Kwak,Chung No Joo ) 생화학분자생물학회 1986 BMB Reports Vol.19 No.1
It was found that ascorbate synthesized during the germination of Glycine max was always much higher than that of control when the soybeans were rinsed in 10-4% ginseng saponin solution at first for 24 hours and the same solution was poured afterwards. Using the homogenate of germinating soybean (2nd day) as enzyme source and glucose containing [U-^(14)C]-glucose as substrate, it was confirmed that the saponin fraction stimulated the ascorbate biosynthesis during germination. From the radioactivity distribution of the intermediates from glucose to ascorbate. it was realized that ascorbate was synthesized from glucose predominantly via galacturonate pathway rather than glucuronate pathway indicating that the glucose was epimerized first to galactose by the action of UDP-glucose epimerase.