망막전위도의 전력 산출

원철호,조진호,김상하,임정훈 경북대학교 의학연구소 1999 경북대학교병원의학연구소논문집 Vol.3 No.1

주파수영역에서 망막전위도의 총전력을 계산하고, 시간영역에서 망막전위도의 면적을계산할수 있는 프로그램을 개발함으로써 전기생리학적 진단의 효능을 향상시키는데 목적을 두었다. 총전력은 망막전위도 신호를 Fourier 변환을 이용하여 주파수 영역의 신호로 바꾼 다음 스펙트럼의 제곱을 적분함으로써 구하였다. 우세전력은 우세주파수의 전력으로 이부분의 전압을 제곱후 적분하여 얻은 값이다. 원하는 부분은 수동입력하여 구간주파수의 제곱적분을 할 수 있게 하였다. 이 소프트웨어는 시간 영역에서 진동양소파의 진폭의 합 및 면적도 구할 수 있는 기능을 가졌으며, 사용자에게 편리하도록 그래픽 환경 하에서 마우스와 팝업 메뉴에 의하여 각 기능들이 작동되며, UTAS-E2000 시스템과도 상호 호환성을 갖고 있다(한안지 38:817∼823, 1997). The Calculation of the Electroretinographic Power Chul Ho Won*, Jin Ho Cho, Ph.D.**, Sang Ha Kim, M. D., Jung Hoon Lim, M. D. To promote the electrophysiologic diagnostic efficiency, the program for calculation of the total power in the frequency domain and of the area in the time domain of the electroretinogram was designed. The total power is calculated by integration of the squared power spectrum obtained by Fourier transform (Parseval theorem) in the time domain. The dominant power is the power of the dominant frequency in power spectrum and is calculated by integration of the squared peak area spectrum. The sectional integration of the square power spectrum can be established by manual input. The implemented software also has the functions that calculate the summed amplitude and area of the oscillatory potentials in the time domain. And it performs each functions by selection of pop-up menu with mouse to use conveniently under graphic interface, compatible with UTAS-E2000 system (J Korean Ophthalmol Soc 38:817∼823, 1997).

金元 四大家에 關한 硏究

宋哲鎬,洪元植,金光湖 慶熙大學校韓醫科大學韓醫學硏究所 1986 慶熙韓醫大論文集 Vol.9 No.-

Study of decreased melanin production through p53 by heme oxygenase-1 inhibitor in normal human melanocytes

( Hee Sun Lim ),( Sun A Jin ),( Jee Bum Lee ),( Seong Jin Kim ),( Seung Chul Lee ),( Young Ho Won ),( Sook Jung Yoon ) 대한피부과학회 2015 대한피부과학회 학술발표대회집 Vol.67 No.2

Background: Heme oxygenase (HO)-1 is induced by oxidative stress and plays important roles in anti-apoptosis and the rapid growth of several solid tumors. p53 has a central role in skin pigmentation and may impact onmelanoma at all stages. However, there has been little study of HO-1 in relation with p53 on melanin production Objectives: To know the effect of HO-1 on melanogenesis through p53 in normal human melanocytes Methods: Human melanocytes (hM) were primarily cultured from foreskin. After incubation, cells were rinsed twice with PBS then transfection with p53 siRNA Results: Melanin content was detected with ELISA and Western blot analysis and RT-PCR of tyrosinase, MITF were performed after ZnPP treatment and p53 transfection. After ZnPP treatment, melanin content was decreased, and tyrosinase and MITF protein levels were decreased. Tyrosinase and MITF mRNA levels were also decreased. However, melanin content and tyrosinase and MITF protein levels were increased after CoPP treatment. After p53 transfection, HO-1 expression was decreased when HO-1 stimulator was treated. In addition, melanin content was decreased, and tyrosinase expression was decreased in Western blot analysis Conclusion: These results suggest that melanogenesis is inhibited by ZnPP by decreased melanin production, tyrosinase and MITF protein and mRNA levels. Furthermore, those inhibitory effects of ZnPP may be dependent on p53 in normal human melanocytes. Therefore, HO-1 may play an important role in melanogensis

Upregulation of heme oxygenase-1 by ginsenoside Ro attenuates lipopolysaccharide-induced inflammation in macrophage cells

Sokho Kim,Myung-Hoon Oh,Bum-Seok Kim,Won-Il Kim,Ho-Seong Cho,Byoung-Yong Park,Chul Park,Gee-Wook Shin,Jungkee Kwon 고려인삼학회 2015 Journal of Ginseng Research Vol.39 No.4

Background: The beneficial effects of ginsenoside species have been well demonstrated in a number of studies. However, the function of ginsenoside Ro (GRo), an oleanane-type saponin, has not been suffi- ciently investigated. Thus, the aim of the present study was to investigate the anti-inflammatory effects of GRo in vitro using the Raw 264.7 mouse macrophage cell line treated with lipopolysaccharide (LPS), and to clarify the possible mechanism of GRo involving heme oxygenase-1 (HO-1), which itself plays a critical role in self-defense in the presence of inflammatory stress. Methods: Raw 264.7 cells were pretreated with GRo (up to 200mM) for 1 h before treatment with 1 mg/ mL LPS, and both cell viability and inflammatory markers involving HO-1 were evaluated. Results: GRo significantly increased cell viability in a dose dependent manner following treatment with LPS, and decreased levels of reactive oxygen species and nitric oxide. GRo decreased inflammatory cytokines such as nitric oxide synthase and cyclooxygenase-2 induced by LPS. Moreover, GRo increased the expression of HO-1 in a dose dependent manner. Cotreatment of GRo with tin protoporphyrin IX, a selective inhibitor of HO-1, not only inhibited upregulation of HO-1 induced by GRo, but also reversed the anti-inflammatory effect of GRo in LPS treated Raw 264.7 cells. Conclusion: GRo induces anti-inflammatory effects following treatment with LPS via upregulation of HO-1.

Upregulation of heme oxygenase-1 by ginsenoside Ro attenuates lipopolysaccharide-induced inflammation in macrophage cells

Kim, Sokho,Oh, Myung-Hoon,Kim, Bum-Seok,Kim, Won-Il,Cho, Ho-Seong,Park, Byoung-Yong,Park, Chul,Shin, Gee-Wook,Kwon, Jungkee The Korean Society of Ginseng 2015 Journal of Ginseng Research Vol.39 No.4

Background: The beneficial effects of ginsenoside species have been well demonstrated in a number of studies. However, the function of ginsenoside Ro (GRo), an oleanane-type saponin, has not been sufficiently investigated. Thus, the aim of the present study was to investigate the anti-inflammatory effects of GRo in vitro using the Raw 264.7 mouse macrophage cell line treated with lipopolysaccharide (LPS), and to clarify the possible mechanism of GRo involving heme oxygenase-1 (HO-1), which itself plays a critical role in self-defense in the presence of inflammatory stress. Methods: Raw 264.7 cells were pretreated with GRo (up to $200{\mu}M$) for 1 h before treatment with 1 mg/mL LPS, and both cell viability and inflammatory markers involving HO-1 were evaluated. Results: GRo significantly increased cell viability in a dose dependent manner following treatment with LPS, and decreased levels of reactive oxygen species and nitric oxide. GRo decreased inflammatory cytokines such as nitric oxide synthase and cyclooxygenase-2 induced by LPS. Moreover, GRo increased the expression of HO-1 in a dose dependent manner. Cotreatment of GRo with tin protoporphyrin IX, a selective inhibitor of HO-1, not only inhibited upregulation of HO-1 induced by GRo, but also reversed the anti-inflammatory effect of GRo in LPS treated Raw 264.7 cells. Conclusion: GRo induces anti-inflammatory effects following treatment with LPS via upregulation of HO-1.

Sulforaphane protects against acetaminophen-induced hepatotoxicity

Noh, Jung-Ran,Kim, Yong-Hoon,Hwang, Jung Hwan,Choi, Dong-Hee,Kim, Kyoung-Shim,Oh, Won-Keun,Lee, Chul-Ho Elsevier 2015 Food and chemical toxicology Vol.80 No.-

<P><B>Abstract</B></P> <P>Oxidative stress is closely associated with acetaminophen (APAP)-induced toxicity. Heme oxygenase-1 (HO-1), an antioxidant defense enzyme, has been shown to protect against oxidant-induced tissue injury. This study investigated whether sulforaphane (SFN), as a HO-1 inducer, plays a protective role against APAP hepatotoxicity <I>in vitro</I> and <I>in vivo</I>. Pretreatment of primary hepatocyte with SFN induced nuclear factor E2-factor related factor (Nrf2) target gene expression, especially HO-1 mRNA and protein expression, and suppressed APAP-induced glutathione (GSH) depletion and lipid peroxidation, which eventually leads to hepatocyte cell death. A comparable effect was observed in mice treated with APAP. Mice were treated with 300 mg/kg APAP 30 min after SFN (5 mg/kg) administration and were then sacrificed after 6 h. APAP alone caused severe liver injuries as characterized by increased plasma AST and ALT levels, GSH depletion, apoptosis, and 4-hydroxynonenal (4-HNE) formations. This APAP-induced liver damage was significantly attenuated by pretreatment with SFN. Furthermore, while hepatic reactive oxygen species (ROS) levels were increased by APAP exposure, pretreatment with SFN completely blocked ROS formation. These results suggest that SFN plays a protective role against APAP-mediated hepatotoxicity through antioxidant effects mediated by HO-1 induction. SFN has preventive action in oxidative stress-mediated liver injury.</P> <P><B>Highlights</B></P> <P> <UL> <LI> SFN pretreatment increases the cell viability against APAP-induced toxicity. </LI> <LI> SFN pretreatment protects depletion of cellular GSH after APAP treatment. </LI> <LI> SFN pretreatment enhances Nrf2 target gene expression, especially HO-1 after APAP treatment. </LI> <LI> SFN has protective effect against APAP overdose-induced liver injury <I>in vivo</I> model as well. </LI> </UL> </P>

UASB 공정에 의한 고농도 축산폐수 처리시 유기물 제거와 메탄생성에 관한 연구

원철희,김승호,박은영,임재명 江原大學校 産業技術硏究所 2002 産業技術硏究 Vol.22 No.B

This research was performed to investigate the COD removal efficiency and methane production in slurry-typed swine wastes using UASB(upflow anaerobic sludge blanket)reactor. The USAB reactor was operated from 0.8 through 3.3days of HRT in a range of 3 to 15 kg TCOD/m³/day of volumetric organic loading rate. The removal rate of TCOD was increased with the increase of the HRT. The removal rate of TCOD at an HRT over 2days, became greater than 68% with the methane contents being from 70 to 80%. Methane production rates were increased from 0.27 to 0.36 m³CH₄/kg CODrem. as HRTs were increased from 0.8 to 3.3days.

벼 재배방법과 규산질 비료 시용이 생육 및 도복에 미치는 영향

金正鎬,吳成煥,李哲遠,宋凡憲,孫錫龍 忠北大學校 農業科學硏究所 2002 農業科學硏究 Vol.19 No.-

본 시험은 벼 도목을 경감시키기 위한 재배대책을 강구하기 위하여 광안벼를 공시하고, 이앙재배와 담수직파재배에서 규산질 비료를 시용함에 따른 도복경감효과를 조사하였던 바 그 결과를 요약하면 다음과 같다 1 간장은 담순표면직파재배에선 규산시용구가 무처리보다 31cm 감소하여 37%가 단축되었으며, 기계이앙재배에서는 규산시용구가 무처리보다 5cm 감소하여 55% 단축되었다 2 하위절간장은 담수표면직파재배에서 규산시용구가 무처리보다 3.1cm 감소하여 36 9% 단축되었으며, 기계이앙재배에서는 규산시용구가 무처리보다12cm 감소하여 11% 단축되었다 3 좌절중은 담수표면직파에서 규산시용구가 73g이 증가하여 35 2%의 증가율을 보였고, 기계이앙재배에서는 규산시용구가 373g 증가하여 9.6%의 증가율을 보였다 4 수량은 담수표면직파재배에서 규산시용구가 무처리보다 30kg이 많아 64%의 증수효과를 나타내었고, 기계이앙재배에서는 규산시용구가 무처리보다50kg이 많아 9%의 증수효과를 나타내었다 This study was carried out to examine the responses of growth and lodging of rice as affected by both transplanting and direct seeding in waterlogging, which was differently applicated amounts of silicate fertlizers The Kwangan cultivar of Japonica type was used The growths including culm and internode lengths and grain yield were investigated in major growth stages and harvest time The culm length in waterlogging direct seeding was reduced in treatments of silicate fertilizers, about 37%, compared to that in the contro1 and it was reduced to 5.5% in the transplanting cultivation The breaking strength of lower internodes of rice were increased to 35.2% and 96% by applying of silicate fertilizers in the water direct seeding and in the transplanting cultivations, respectively The rice yields were increased with application of silicate fertilizers in both water direct seeding and transplanting cultivations compared to those of the control, about 64% and 90%, respectively.

새로운 Ca^2+ 길항제의 혈압강하효과

함원훈,임태균,정윤성,정윤호,양재권,최원철 성균관대학교 약학연구소 1993 成均藥硏論文集 Vol.5 No.1

Diltiazem and nifedipine, have been used throughout the world as an effective antianginal and antihypertensive agent. In order to increase the activity of these drugs, we synthesized the diltiazem-nifedipine coupling compounds. These compounds^(1-8) were tested for antihypertensive effect. Unfortunately, these compounds didn't show any promising activities.

Bi₂Sr₂Ca(Cu_1-xNix)₂O_8+δ단결정의 전류 변화에 대한 활성화 에너지

손인호,김영국,전칠원,문성희,오재근 경남대학교 공업기술연구소 2000 硏究論文集 Vol.18 No.-

Bi□Sr□Ca(Cu□-□Ni□)□O□+δ (x=0, 0.003) 단결정을 개선된 방향성 응고법을 이용하여 성장시켰으며, 표준 4단자법으로 ab-면의 비저항을 측정하였고, Direct four-probe법을 이용해서 c-축의 비저항을 측정하였다. Arrhenius plot slopes의 기울기는 활성화 에너지를 나타내는데 이의 온도 의존성에 대하여 조사하였다. 자기장을 인가하여 활성화 에너지를 측정하면, 고온과 저온에 서로 다른 두개의 TAFF 영역이 나타나는 것을 볼 수 있고, 이들 활성화에너지는 고온 영역에서는 lnB에 비례하고, 저온 영역에서는 B□□ 에 비례하였다. By making use of the improved directional solidification method and, standard and direct four-prove methods respectively. We grow Bi□Sr□Ca(Cu1-□Ni□)2O8+δ (x=0, 0.003) single crystal and measure its resitivities in ab-plane and c-axis. The temperature at the activation energy, which is described by a slope in the Arrhenius plot, is examined. The activation energy measured by applying the external magnetic field, exhibits two different TAFF regimes at low and high temperatures. It is shown that the activation energy is propotional to lnB and B□□ at high and low temperature regimes, respectively.