Novel Anticandidal Activity of a Recombinant Lampetra japonica RGD3 Protein

( Cai Ping Wu ),( Li Lu ),( Yuan Yuan Zheng ),( Xin Liu ),( Rong Xiao ),( Ji Hong Wang ),( Qing Wei Li ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.7

Lj-RGD3, an RGD (Arg-Gly-Asp) toxin protein from the salivary gland of Lampetra japonica,exhibits antifungal activity against Candida albicans. Lj-RGD3 has three RGD motifs and shows homology to histidine-rich glycoprotein. We synthesised two mutant derivatives of Lj-RGD3: Lj-26, which lacks all three RGD motifs and contains no His residues; and Lj-112, which lacks only the three RGD motifs. We investigated the effects of the wild-type and mutated toxins on a gram-positive bacterium (Escherichia coli), a gram-negative bacterium (Staphylococcus aureus), and a fungus (C. albicans). rLj-RGD3 and its mutants exhibited antifungal but not antibacterial activity, as measured by a radial diffusion assay. The C. albicans inhibition zone induced by rLj-112 was larger than that induced by the other proteins, and its inhibitory effect on C. albicans was dose-dependent. In viable-count assays, the rLj-112 MIC was 7.7 μM, whereas the MIC of the positive control (ketoconazole) was 15 μM. Time-kill kinetics demonstrated that rLj-112 effectively killed C. albicans at 1× and 2× MIC within 12 and 6 h, respectively. Electron microscopy analysis showed that rLj-RGD3 and rLj-112 induced C. albicans lysis. Our results demonstrate a novel anticandidal activity for rLj-RGD3 and its mutant derivatives.

A coupled finite element/meshfreemoving boundary method for self-piercing riveting simulation

Cai, Wayne,Wang, Hui-Ping,Wu, C.T. Techno-Press 2013 Interaction and multiscale mechanics Vol.6 No.2

The use of lightweight materials has been steadily increasing in the automotive industry, and presents new challenges to material joining. Among many joining processes, self-piercing riveting (SPR) is particularly promising for joining lightweight materials (such as aluminum alloys) and dissimilar materials (such as steel to Al, and metal to polymer). However, to establish a process window for optimal joint performance, it often requires a long trial-and-error testing of the SPR process. This is because current state of the art in numerical analysis still cannot effectively resolve the problems of severe material distortion and separation in the SPR simulation. This paper presents a coupled meshfree/finite element with a moving boundary algorithm to overcome these numerical difficulties. The simulation results are compared with physical measurements to demonstrate the effectiveness of the present method.

Laparoscopic surgery contributes to a decrease in short-term complications in surgical ulcerative colitis patients during 2008–2017: a multicenter retrospective study in China

Zerong Cai,Xiaosheng He,Jianfeng Gong,Peng Du,Wenjian Meng,Wei Zhou,Jinbo Jiang,Bin Wu,Weitang Yuan,Qi Xue,Lianwen Yuan,Jinhai Wang,Jiandong Tai,Jie Liang,Weiming Zhu,Ping Lan,Xiaojian Wu 대한장연구학회 2023 Intestinal Research Vol.21 No.2

Background/Aims: The aim of this study was to analyze the chronological changes in postoperative complications in surgical ulcerative colitis patients over the past decade in China and to investigate the potential parameters that contributed to the changes. Methods: Ulcerative colitis patients who underwent surgery during 2008–2017 were retrospectively enrolled from 13 hospitals in China. Postoperative complications were compared among different operation years. Risk factors for complications were identified by logistic regression analysis. Results: A total of 446 surgical ulcerative colitis patients were analyzed. Fewer short-term complications (24.8% vs. 41.0%, P=0.001) and more laparoscopic surgeries (66.4% vs. 25.0%, P<0.001) were found among patients who received surgery during 2014–2017 than 2008–2013. Logistic regression suggested that independent protective factors against short-term complications were a higher preoperative body mass index (odds ratio [OR], 0.870; 95% confidence interval [CI], 0.785–0.964; P=0.008), laparoscopic surgery (OR, 0.391; 95% CI, 0.217–0.705; P=0.002) and elective surgery (OR, 0.213; 95% CI, 0.067–0.675; P=0.009). The chronological decrease in short-term complications was associated with an increase in laparoscopic surgery. Conclusions: Our data revealed a downward trend of short-term postoperative complications among surgical ulcerative colitis patients in China during the past decade, which may be due to the promotion of minimally invasive techniques among Chinese surgeons.

Mutations of ARX and non-syndromic intellectual disability in Chinese population

Yufei Wu,Huan Zhang,Xiaofen Liu,Zhangyan Shi,Hongling Li,Zhibin Wang,Xiaoyong Jie,Shao-Ping Huang,Fu-Chang Zhang,Junlin Li,Ke-Jin Zhang,Xiao-Cai Gao 한국유전학회 2019 Genes & Genomics Vol.41 No.1

Mutations of Aristaless-related homeobox (ARX) gene were looked as the third cause of non-syndromic intellectual disability (NSID), while the boundary between true disease-causing mutations and non-disease-causing variants within this gene remains elusive. To investigate the relationship between ARX mutations and NSID, a panel comprising six reported causal mutations of the ARX was detected in 369 sporadic NSID patients and 550 random participants in Chinese. Two mutations, c.428_451 dup and p.G286S, may be disease-causing mutations for NSID, while p.Q163R and p.P353L showed a great predictive value in female NSID diagnosis with significant associations (X2 = 19.60, p = 9.54e−6 for p.Q163R; X2 = 25.70, p = 4.00e−07 for p.P353L), carriers of these mutations had an increased risk of NSID of more than fourfold. Detection of this panel also predicted significant associations between genetic variants of the ARX gene and NSID (p = 3.73e−4). The present study emphasized the higher genetic burden of the ARX gene on NSID in the Chinese population, molecular analysis of this gene should be considered for patients presenting NSID of unknown etiology.

Flavonoids of Rosa roxburghii Tratt Act as Radioprotectors

Xu, Ping,Zhang, Wen-Bo,Cai, Xin-Hua,Lu, Dan-Dan,He, Xiao-Yang,Qiu, Pei-Yong,Wu, Jiao Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.19

Background: To study the radioprotective effects of flavonoids from Rosa roxburghii Tratt (FRT). Materials and Methods: The radioprotective effects of FRT were investigated by examining cell viability, 30-day survival of mice and the number of colony-forming units in spleen (CFU-S) after total-body 60Co irradiation. Results: The survival rates of irradiated cells gradually increased with increasing concentrations of FRT. The survival rate was the highest at 87% with a concentration of $30{\mu}g/mL$. Pretreatment with FRT was needed to realize its radioprotective activity in mice at the dose of 60 mg/kg. With the increasing doses of 30 mg/kg, 60 mg/kg and 120 mg/kg, the numbers of CFU-S increased, and were significantly different compared with the control group. Conclusions: Pretreatment with FRT prior to irradiation resulted in significantly higher cell survival at 24 h after 5 Gy radiation, increased 30-day survival in mice after exposure to a potentially lethal dose of 8 Gy, and resulted in a higher number of CFU-S in mice after exposure to a dose of 6 Gy. These results collectively indicate that FRT is an effective radioprotective agent.

Glutamine-Supplemented Parenteral Nutrition and Probiotics in Four Adult Autoimmune Enteropathy Patients

( Ren Ying Xu ),( Yan Ping Wan ),( Yi Quan Zhou ),( Li Ping Lu ),( Zhi Qi Chen ),( Ying Jie Wu ),( Wei Cai ) 대한소화기학회 2014 Gut and Liver Vol.8 No.3

To evaluate the effects of glutamine-supplemented parenteral nutrition (PN) and probiotics in adult autoimmune enteropathy (AIE) patients. Four adult AIE patients were identified from April 2006 to January 2012. Clinical and nutritional data were obtained from the patients’ medical records. Glutamine-supplemented PN started immediately when the AIE diagnosis was confirmed. The total PN duration was 351 days. According to the PN prescription, the average caloric intake ranged from 20 to 25 kcal/kg/day, and the protein intake ranged from 1.2 to 1.5 g/kg/day. Alanyl-glutamine (20 g/day) was administered to AIE patients for 4 weeks followed by a 2-week break, and this treatment schedule was repeated when PN lasted for more than 6 weeks. Body weight gain and an increased serum albumin level were achieved after PN, and defecation frequency and quality also improved. Each patient received oral supplements, 250 mL of Ensure and two probiotics capsules (each capsule containing 0.5×108 colonies) three times a day when enteral nutrition started. Three AIE patients were successfully weaned off PN, and one patient died of pneumonia. Glutamine-supplemented PN and probiotics show promise in managing patients with AIE and related malnutrition.

Mitigating SYN flooding Attack and ARP Spoofing in SDN Data Plane

Ting-Yu Lin,Jhen-Ping Wu,Pei-Hsuan Hung,Ching-Hsuan Shao,Yu-Ting Wang,Yun-Zhan Cai,Meng-Hsun Tsai 한국통신학회 2020 한국통신학회 APNOMS Vol.2020 No.09

As the number of network devices increases rapidly, it becomes more and more difficult to defend network attacks. Large-scaled attacks, such as SYN flooding, may lead to heavy burden to the switches as well as the controller in a software defined network (SDN). In this paper, we investigate the SYN flooding and Address Resolution Protocol (ARP) spoofing attacks in SDN, and then propose mechanisms to address these two attacks. We also present a new scheme to detect SYN flooding by using only a few forwarding rules. Moreover, we utilize the Programming Protocol-independent Packet Processors (P4) technique to mitigate the burden of the controller.

On the Strong Convergence Theorems for Asymptotically Nonexpansive Semigroups in Banach Spaces

Shih-sen Chang,Liang Cai Zhao,Ding Ping Wu 한국전산응용수학회 2009 Journal of applied mathematics & informatics Vol.27 No.1

Some strong convergence theorems of explicit iteration scheme for asymptotically nonexpansive semi-groups in Banach spaces are estab- lished. The results presented in this paper extend and improve some re- cent results in [T. Suzuki. On strong convergence to common fixed points of nonexpansive semigroups in Hilbert spaces, Proc. Amer. Math. Soc. 131(2002)2133–2136; H. K. Xu. A strong convergence theoremfor contrac- tion semigroups in Banach spaces, Bull. Aust. Math. Soc. 72(2005)371– 379; N. Shioji and W. Takahashi. Strong convergence theorems for con- tinuous semigroups in Banach spaces, Math. Japonica. 1(1999)57–66; T. Shimizu and W. Takahashi. Strong convergence to common fixed points of families of nonexpansivemappings, J. Math. Anal. Appl. 211(1997)71–83; N. Shioji and W. Takahashi. Strong convergence theorems for asymptot- ically nonexpansive mappings in Hilbert spaces, Nonlinear Anal. TMA, 34(1998)87–99; H. K. Xu. Approximations to fixed points of contraction semigroups in Hilbert space, Numer. Funct. Anal. Optim. 19(1998), 157–163.] Some strong convergence theorems of explicit iteration scheme for asymptotically nonexpansive semi-groups in Banach spaces are estab- lished. The results presented in this paper extend and improve some re- cent results in [T. Suzuki. On strong convergence to common fixed points of nonexpansive semigroups in Hilbert spaces, Proc. Amer. Math. Soc. 131(2002)2133–2136; H. K. Xu. A strong convergence theoremfor contrac- tion semigroups in Banach spaces, Bull. Aust. Math. Soc. 72(2005)371– 379; N. Shioji and W. Takahashi. Strong convergence theorems for con- tinuous semigroups in Banach spaces, Math. Japonica. 1(1999)57–66; T. Shimizu and W. Takahashi. Strong convergence to common fixed points of families of nonexpansivemappings, J. Math. Anal. Appl. 211(1997)71–83; N. Shioji and W. Takahashi. Strong convergence theorems for asymptot- ically nonexpansive mappings in Hilbert spaces, Nonlinear Anal. TMA, 34(1998)87–99; H. K. Xu. Approximations to fixed points of contraction semigroups in Hilbert space, Numer. Funct. Anal. Optim. 19(1998), 157–163.]

Multi-Index Seismic Capacity Evaluation of Buckling-Restrained Braced Frames

Yulong Feng,Jing Wu,Xiaoning Cai,Shao-ping Meng 한국강구조학회 2018 International Journal of Steel Structures Vol.18 No.2

The seismic capacity of a structure is determined by the performance index that reaches its ultimate bearing or deformation capacity fi rst. This paper presents a multi-index seismic capacity evaluation method for accurately evaluating the seismic capacity of a structure. The normalized response curves of several indices are concurrently plotted to form a multi-index seismic capacity evaluation fi gure, in which the seismic capacity and demand values that correspond to various indices can be determined by vertical and horizontal threshold lines. Based on an incremental dynamic analysis (IDA), a 6-story buckling-restrained braced frame (BRBF) and a series of comparable 6-story steel frames are analyzed using the proposed method to verify the method and investigate the seismic behavior of BRBFs. The results show that the seismic performance of buckling-restrained braces is not the only factor that determines the seismic capacity of BRBFs and indicate that the multiindex seismic capacity evaluation method can eff ectively identify the critical index of a structure and the weakest links that restrict the structural seismic capacity.

Triterpenoids from Schisandra henryi with Cytotoxic Effect on Leukemia and Hela Cells In Vitro

Chen, Ye-Gao,Wu, Zheng-Cai,Lv, Yu-Ping,Gui, Shi-Hong,Wen, Jin,Liao, Xin-Rong,Yuan, Li-Ming,Halaweish, Fathi The Pharmaceutical Society of Korea 2003 Archives of Pharmacal Research Vol.26 No.11

Four known lanostane triterpenoids, schiprolactone A (1), schisanlactone B (2), nigranoic acid (3) and schisandronic acid (4) Were isolated from the stems of Schisandra henryi for the first time. Their structures were characterized by IR, MS and NMR techniques. Compounds 1, 2 and 4 showed moderate cytotoxic activity against Leukemia cells in vitro. Cytotoxic activity of compounds 1-4 showed $IC_{50}$ of 0.0097, 0.01, 0.097 and 0.0099 $\mu$ mol/mL respectively toward Leukemia cells and $IC_{50}$ of 0.097, 0.1, 0.097 and 0.099 $\mu$mol/mL toward Hela cells respectively. It is the first report that these compounds possess cytotoxic activity on Leukemia and Hela cells.