Madecassic acid protects human periodontal ligament fibroblasts against hydrogen peroxide-induced cell damage by maintaining mitochondrial membrane potential

Jin Yuqin,Li Jialing,Ding Liang,Zhao Qing,Song Yuxian,Li Guifeng,Ji Jun,Ni Yanhong,Hu Qingang 대한독성 유전단백체 학회 2022 Molecular & cellular toxicology Vol.18 No.1

Background Oxidative stress is involved in the pathogenesis of various inflammatory diseases, such as periodontitis. When periodontitis occurs, reactive oxygen species (ROS) are overproduced and cannot be balanced by the antioxidant defense system, resulting in tissue damage. Madecassic acid (MA), an abundant triterpenoid in Centella asiatica (L.) Urban, has been used as a wound healing, antiinflammatory, and anticancer agent. Moreover, recent studies have shown that MA has an antioxidative effect, but the underlying mechanism remains unclear. Objective Here, we established an effective oxidative stress model induced by hydrogen peroxide (H 2 O 2 ) in human periodontal ligament fi broblasts (hPDLFs) to investigate the antioxidant and protective effects of MA against cell damage and its underlying mechanism of action. Results Pretreatment with MA inhibited cell apoptosis and promoted cell invasion and migration against oxidative injury induced by H 2 O 2 . In addition, MA was able to maintain mitochondrial membrane potential (ΔΨm) under oxidative stress. Notably, we found that MA restored redox balance by reducing intracellular ROS production. Furthermore, we investigated apoptosis-related proteins and found that the levels of anti-apoptosis markers Bcl-xL and Bcl-2 were remarkably upregulated, whereas that of the pro-apoptotic marker Bax was strikingly downregulated. Conclusions Collectively, these findings suggest that MA inhibits H 2 O 2 -induced oxidative stress and apoptosis of hPDLFs by reducing intracellular ROS production to maintain ΔΨm stability.

Acteoside and Acyl-Migrated Acteoside, Compounds in Chinese Kudingcha Tea, Inhibit α-Amylase In Vitro

Yuqin Lu,Wenyu Zhou,Yue Feng,Yao Li,Ke Liu,Lizhong Liu,Dongxu Lin,Zhendan He,Xuli Wu 한국식품영양과학회 2017 Journal of medicinal food Vol.20 No.6

Acteoside, the predominant polyphenol of small-leaved kudingcha, the Chinese tea, has various biological activities. In this study, we examined the acyl migration of acteoside to isoacteoside with high-temperature treatment of acteoside. The inhibitory effects of acyl-migrated acteoside and acteoside on α-amylase were investigated, as were their binding interaction with α-amylase. The binding of acteoside and isoacteoside to α-amylase was investigated by using the fluorescence spectra assay, circular dichroism, and protein–ligand docking studies. Acteoside was more effective than preheated acteoside and isoacteoside in inhibiting α-amylase activity. Acteoside and isoacteoside binding to α-amylase may induce conformational changes to α-amylase, and the binding site of acteoside and isoacteoside being near the active site pocket of α-amylase may explain the decreased activity of α-amylase. The different affinities and binding sites of acteoside and isoacteoside for α-amylase resulted in different inhibition rates, which may be due to structural differences between acteoside and isoacteoside.

Effects of feeding different levels of dietary corn silage on growth performance, rumen fermentation and bacterial community of post-weaning dairy calves

Li Lingyan,Qu Jiachen,Zhu Huan,Liu Yuqin,Wu Jianhao,Shao Guang,Guan Xianchao,Qu Yongli 아세아·태평양축산학회 2024 Animal Bioscience Vol.37 No.2

Objective: The objective of this study was to evaluate the growth performance, rumen fermentation parameters and bacterial community of post-weaning dairy calves in response to five diets varying in corn silage (CS) inclusion.Methods: A total of forty Holstein weaned bull calves (80±3 days of age;128.2±5.03 kg at study initiation) were randomized into five groups (8 calves/group) with each receiving one of five dietary treatments offered as total mixed ration in a 123-d feeding study. Dietary treatments were control diet (CON; 0% CS dry matter [DM]); Treatment 1 (T1; 27.2% CS DM); Treatment 2 (T2; 46.5% CS DM); Treatment 3 (T3; 54.8% CS DM); and Treatment 4 (T4; 67.2% CS DM) with all diets balanced for similar protein and energy concentration.Results: Results showed that calves offered CS had greater average daily gain, body length and chest depth growth, meanwhile altered rumen fermentation indicated by decreased rumen acetate concentrations. Principal coordinate analysis showed the rumen bacterial community structure was affected by varying CS inclusion diets. <i>Bacteroidetes</i> and <i>Firmicutes</i> were the predominant bacterial phyla in the calf rumens across all treatments. At the genus level, the abundance of <i>Bacteroidales_RF16_group</i> was increased, whereas <i>Unclassified_ Lachnospiraceae</i> was decreased for calves fed CS. Furthermore, Spearman’s correlation test between the rumen bacteria and rumen fermentation parameters indicated that <i>Bacteroidales_RF16_group</i> and <i>Unclassified Lachnospiraceae</i> were positively correlated with propionate and acetate, respectively.Conclusion: The results of the current study suggested that diet CS inclusion was beneficial for post-weaning dairy calf growth, with 27.2% to 46.5% CS of diet DM recommended to achieve improved growth performance. <i>Bacteroidales_RF16_group</i> and <i>Unclassified Lachnospiraceae</i> play an important role in the rumen fermentation pattern for post-weaning calves fed CS.

Polymorphism of Exon 2 of BMP15 Gene and Its Relationship with Litter Size of Two Chinese Goats

Wang, Yuqin,Yuanxiao, Li,Nana, Zhang,Zhanbin, Wang,Junyan, Bai Asian Australasian Association of Animal Productio 2011 Animal Bioscience Vol.24 No.7

Polymorphisms of BMP15 gene exon 2 and its relationship with prolificacy of goats were detected by PCR-SSCP and DNA sequencing methods in Chinese two local goat breeds. The results showed that the product amplified by the primers displayed polymorphisms. Three genotypes (AA, BB and AB) were detected in Funiu white goats, and their frequency was 0.071, 0.715, 0.214, respectively. Two genotypes (AB and BB) were detected in Taihang black goats, and their frequency was 0.342 and 0.658, respectively. Sequencing revealed that four mutations (456T${\rightarrow}$G, 466C${\rightarrow}$G, 510C${\rightarrow}$T, 511T${\rightarrow}$C) occurred in genotype BB of Funiu white goat, which resulted in amino acid substitution of V155G and S171P. No mutation was detected in Taihang black goat. The Funiu white goat with genotype BB had 0.91 or 0.82 kids, more than those with AB or AA, respectively. The difference of the least squares means for litter size between BB and AB was not significant (p>0.05) in Taihang black goat. It is concluded that the BMP15 gene may be a major gene which affects the prolificacy in Funiu white goats. This study could provide basic molecular data on the reproductive characteristics of local breeds of Henan province in China, and a scientific basis for the conservation and utilization of those two goat breeds.

Influence of Hybrid Ratios on Shape Memory Properties of Basalt/Carbon Fiber Hybrid Composites with Graphene Oxide Prepared by VIHPS

Yi Xu,Yuqin Ma,Yu Yang,Gangfeng Wang,Wei Xu,Fei Li,Haiyin Guo,Yatao Li 한국섬유공학회 2023 Fibers and polymers Vol.24 No.8

A study on the shape memory properties of 6 layers bi-directional basalt fiber/carbon fiber hybrid composites with graphene oxide (GO) was presented in this paper. The hybrid composites were prepared by the vacuum infiltration hot pressing system (VIHPS). The macroscopic appearance of these composites was desirable, and their microstructure was satisfactory. According to the test results, with the content of basalt fiber decreased, the shape fixation ratio decreased, while the shape recovery ratio and shape recovery force all increased. The basalt fiber composite with 6 layers basalt fiber (B6) had a maximum shape fixation ratio of 94.94%, a minimum shape recovery ratio of 85.52%, and a minimum recovery force of 1.36 N. Compared with B6, the shape fixation ratio of the carbon fiber composite with 6 layers carbon fiber (C6) decreased by 10.43%, which was 84.51%. The shape recovery ratio and shape recovery force were increased by 9.60% and 3.31 times, and their values were 95.12% and 5.86 N, respectively. In addition, according to the temperature-recovery force curves, it was found that the seven groups of composites all reached their maximum shape recovery force when the temperature was about 110 °C. When the temperature was between 50 °C and 80 °C, the recovery force of the composite increased greatly, and then, the recovery force increased slightly. However, when the temperature was above the glass transition temperature (Tg), the recovery force gradually decreased.

Structure Design and Analysis of a Compact Breast Biopsy Robot under MRI Environment

Yongde Zhang,Haiyan Du,Yuqin Li,Hongxia Zhang 보안공학연구지원센터(IJSIP) 2014 International Journal of Signal Processing, Image Vol.7 No.2

GM-CSF augmented the photothermal immunotherapeutic outcome of self-driving gold nanoparticles against colorectal cancer

Jie Dai,Jianmei Li,Yuqin Zhang,Qian Wen,Yun Lu,Yu Fan,Fancai Zeng,Zhiyong Qian,Yan Zhang,Shaozhi Fu 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00

Background Hypoxia is a frequent characteristic observed in solid tumors and is strongly associated with tumor metastasis, angiogenesis, and drug resistance. While the vasculature of hypoxic tumor tissues poses obstacles to the efficient administration of conventional drugs, it may prove advantageous in sustaining hyperthermia. Photothermal therapy (PTT) offers a promising treatment strategy that utilizes the activation of photosensitizers to produce heat, thus facilitating the selective ablation of tumor tissues. Method To enhance the accumulation of photothermal agents in tumor tissue and improve the effectiveness of PTT, we developed a self-propelled hybrid called Bif@PAu-NPs. This hybrid consists of polydopamine (PDA)-coated gold nanoparticles (Au-NPs) loaded onto the anaerobic Bifidobacterium infantis (Bif ). Results The Bif@PAu-NPs actively aggregated at the tumor site because the ability of Bif can target hypoxic regions, and PAu-NPs achieved precise PTT due to their high photothermal conversion efficiency (η = 67.8%). The tumor tissues were ablated by PTT, resulting in the release of antigens through immunogenic cell death (ICD), which stimulates an immune response. The inclusion of GM-CSF enhanced the immune response by recruiting dendritic cells and initiating long-term anti-tumor immunity. Conclusion The Bif@PAu-NPs hybrid effectively suppressed the growth of both primary tumors and re-challenged tumors. The utilization Bif@PAu-NPs in conjunction with GM-SCF exhibits great potential as a photothermal-immunotherapeutic strategy for precisely treating solid tumors.

Influence of Ligustrum lucidum and Schisandra chinensis Fruits on Antioxidative Metabolism and Immunological Parameters of Layer Chicks

Ma, Deying,Liu, Yuqin,Liu, Shengwang,Li, Qundao,Shan, Anshan Asian Australasian Association of Animal Productio 2007 Animal Bioscience Vol.20 No.9

The experiment was conducted to evaluate the effects of Ligustrum lucidum (LL) and Schisandra chinensis (SC) on the growth, antioxidative metabolism and immunity of laying strain male chicks. The results showed that diets supplemented with 1% of either LL or SC had no effects on the growth performance of chicks compared with the control. Furthermore, both LL and SC significantly reduced malondialdehyde (MDA) concentration of serum and heart of chicks (p<0.05). In addition, superoxide dismutase (SOD) activity of serum of the birds was significantly elevated by supplementation with SC (p<0.05). Glutathione reductase (GR) activity of heart and serum of the birds was significantly elevated by supplementation with LL or SC (p<0.05). LL supplementation significantly elevated antibody values against Newcastle Disease virus (NDV)(p<0.05) and lymphoblastogenesis (p<0.05) of the birds. The results suggest that diets supplemented with 1% of either LL or SC may improve immune function and antioxidant status of chicks.

Tissue distribution of marbofloxacin in pigs after a single intramuscular injection

Fan Yang,Yiming Liu,Zhili Li,Yuqin Wang,Baobao Liu,Zhensheng Zhao,Bianhua Zhou,Guoyong Wang 대한수의학회 2017 Journal of Veterinary Science Vol.18 No.2

Tissue distribution of marbofloxacin was studied in pigs after a single intramuscular injection at 2.5 mg/kg body weight. Samples of plasma, muscle, liver, kidney, heart, lung, and muscle at the injection site were randomly collected from five pigs at 2, 6, 10, 24, 48, 72, and 96 h after administration. Marbofloxacin concentrations were determined by using high-performance liquid chromatography with ultraviolet detection and were subjected to non-compartmental analysis to obtain kinetic parameters. The elimination half-life (t1/2lz) of marbofloxacin at the injection site was 22.12 h, while those in kidney, plasma, liver, lung, heart, and muscle were 16.75, 21.48, 21.84, 24.00, 24.45, and 28.91 h, respectively. Areas under the concentration-time curve from 0 h to ∞ (AUC0–∞s) were calculated to be 31.17 hㆍmgㆍmL−1 for plasma and 32.97, 33.92, 34.78, 37.58, 42.02, and 98.80 hㆍmgㆍg−1 for heart, muscle, lung, liver, kidney, and injection site, respectively. The peak concentration (Cmax) of marbofloxacin was 1.62 µg/mL in plasma and 1.71, 1.74, 1.86, 1.93, 2.45, and 7.64 µg/g in heart, lung, muscle, kidney, liver, and injection site, respectively. The results show that marbofloxacin was fast absorbed, extensively distributed, and slowly eliminated from pigs after a single intramuscular administration.