Remote Identification for Smart-meter Operation Error based on a Wireless Sensor Network in a Smart City

Wenyu Liu,Haishan Zhou,Muxin Zhang,Ying Shang,Yudan Liu 대한전자공학회 2022 IEIE Transactions on Smart Processing & Computing Vol.11 No.6

This paper presents a remote identification method for smart-meter operation errors based on a wireless sensor network (WSN) in a smart city to reduce the bad operation of smart meters. In the WSN, ZigBee communication technology was used to collect data from smart meters to enhance the reliability of information. The LEACH protocol can effectively extend the network life cycle and was selected as a communication protocol for WSNs to collect operation data from intelligent meters. Based on the operation data of a smart meter collected by a WSN, a remote identification model of operation error of a smart meter was established. The limited memory least square algorithm was adopted to construct the remote identification model of operation error for a smart meter to realize the remote identification of a smart-meter error in a smart city. The proposed method can use a WSN to collect operation data from a smart meter and remotely identify the mean absolute percentage error (MAPE) and root mean square error (RMSE) of a smart meter’s operation error, which were less than 1%.

An Engineered Outer Membrane-Defective Escherichia coli Secreting Protective Antigens against Streptococcus suis via the Twin-Arginine Translocation Pathway as a Vaccine

( Wenyu Li ),( Fan Yin ),( Zixuan Bu ),( Yuying Liu ),( Yongqing Zhang ),( Xiabing Chen ),( Shaowen Li ),( Lu Li ),( Rui Zhou ),( Qi Huang ) 한국미생물 · 생명공학회 2022 Journal of microbiology and biotechnology Vol.32 No.3

Live bacterial vector vaccines are one of the most promising vaccine types and have the advantages of low cost, flexibility, and good safety. Meanwhile, protein secretion systems have been reported as useful tools to facilitate the release of heterologous antigen proteins from bacterial vectors. The twin-arginine translocation (Tat) system is an important protein export system that transports fully folded proteins in a signal peptide-dependent manner. In this study, we constructed a live vector vaccine using an engineered commensal Escherichia coli strain in which amiA and amiC genes were deleted, resulting in a leaky outer membrane that allows the release of periplasmic proteins to the extracellular environment. The protective antigen proteins SLY, enolase, and Sbp against Streptococcus suis were targeted to the Tat pathway by fusing a Tat signal peptide. Our results showed that by exploiting the Tat pathway and the outer membrane-defective E. coli strain, the antigen proteins were successfully secreted. The strains secreting the antigen proteins were used to vaccinate mice. After S. suis challenge, the vaccinated group showed significantly higher survival and milder clinical symptoms compared with the vector group. Further analysis showed that the mice in the vaccinated group had lower burdens of bacteria load and slighter pathological changes. Our study reports a novel live bacterial vector vaccine that uses the Tat system and provides a new alternative for developing S. suis vaccine.

Circular RNA expression profile of systemic lupus erythematosus and its clinical significance as a potential novel biomarker

Li Wenyu,Fan Runge,Zhou Cheng,Wei Yue,Lin Shunsheng,Wen Sijian,Zeng Wen,Hou Wei,Zhao Cheng,Lin Youkun 한국유전학회 2022 Genes & Genomics Vol.44 No.11

Background: Circular RNAs (circRNAs) are a class of endogenous noncoding RNAs that are more abundant, specific, and highly organized than linear RNAs. Increasing evidence supports that circRNAs may serve as diagnostic biomarkers in many diseases, but their potential as biomarkers in systemic lupus erythematosus (SLE) remains unclear. Objective: We investigated the critical circRNAs involved in SLE progression and explored their potential application as biomarkers in SLE. Method: RNA sequencing was conducted on peripheral blood mononuclear cells (PBMCs) from 4 SLE patients and 4 healthy volunteers. CircRNA profile data were analyzed to identify differentially expressed circRNAs and visualized via R software. After screening, qPCR analysis of target circRNA expression was performed using PBMCs from 31 SLE patients and 35 healthy volunteers. Correlations between circRNA expression levels and the SLEDAI score were assessed via Spearman correlation analysis. Finally, the performance of circRNAs as biomarkers in SLE was examined by receiver operating characteristic curve analysis. Results: The result identified six differentially expressed circRNAs between SLE patients and healthy controls: hsa_circ_0006689, hsa_circ_0070562, hsa_circ_0006117, hsa_circ_0007683, hsa_circ_0042519, and hsa_circ_0008647. The validation analysis showed differing relative expression levels of hsa_circ_0007683, hsa_circ_0042519, hsa_circ_0008647, and hsa_circ_0006689 between SLE patients and healthy volunteers (P < 0.05), and hsa_circ_0006689 expression in PBMCs correlated with the SLEDAI score (P < 0.05). Furthermore, addition of hsa_circ_0006689 expression increased the sensitivities of anti-dsDNA antibody and anti-Sm antibody levels for SLE diagnosis (from 29.03 to 61.30% and 32.26-71.00%, respectively). Conclusion: Our results suggest hsa_circ_0006689 may be a useful circRNA biomarker for SLE diagnosis and prognosis.

Acteoside and Acyl-Migrated Acteoside, Compounds in Chinese Kudingcha Tea, Inhibit α-Amylase In Vitro

Yuqin Lu,Wenyu Zhou,Yue Feng,Yao Li,Ke Liu,Lizhong Liu,Dongxu Lin,Zhendan He,Xuli Wu 한국식품영양과학회 2017 Journal of medicinal food Vol.20 No.6

Acteoside, the predominant polyphenol of small-leaved kudingcha, the Chinese tea, has various biological activities. In this study, we examined the acyl migration of acteoside to isoacteoside with high-temperature treatment of acteoside. The inhibitory effects of acyl-migrated acteoside and acteoside on α-amylase were investigated, as were their binding interaction with α-amylase. The binding of acteoside and isoacteoside to α-amylase was investigated by using the fluorescence spectra assay, circular dichroism, and protein–ligand docking studies. Acteoside was more effective than preheated acteoside and isoacteoside in inhibiting α-amylase activity. Acteoside and isoacteoside binding to α-amylase may induce conformational changes to α-amylase, and the binding site of acteoside and isoacteoside being near the active site pocket of α-amylase may explain the decreased activity of α-amylase. The different affinities and binding sites of acteoside and isoacteoside for α-amylase resulted in different inhibition rates, which may be due to structural differences between acteoside and isoacteoside.

An evaluation of heat on protein oxidation of soy protein isolate or soy protein isolate mixed with soybean oil in vitro and its consequences on redox status of broilers at early age

Xianglun Zhang,Peng Lu,Wenyue Xue,Dawei wu,Chao Wen,Yanmin Zhou 아세아·태평양축산학회 2017 Animal Bioscience Vol.30 No.8

Objective: The objective of this study was to evaluate effects of heat treatment and soybean oil inclusion on protein oxidation of soy protein isolate (SPI) and of oxidized protein on redox status of broilers at an early age. Methods: SPI mixed with soybean oil (SPIO) heated at 100°C for 8 h was used to evaluate protein oxidation of SPI. A total of two hundred and sixteen 1-day-old Arbor Acres chicks were divided into 3 groups with 6 replicates of 12 birds, receiving basal diet (CON), heat-oxidized SPI diet (HSPI) or mixture of SPI and 2% soybean oil diet (HSPIO) for 21 d, respectively. Results: Increased protein carbonyl, decreased protein sulfhydryl of SPI were observed as heating time increased in all treatments (p<0.05). Addition of 2% soybean oil increased protein carbonyl of SPI at 8 h heating (p<0.05). Dietary HSPI and HSPIO decreased the average daily gain of broilers as compared with the CON (p<0.05). Broilers fed HSPI and HSPIO exhibited decreased glutathione (GSH) in serum, catalase activity and total sulfhydryl in liver and increased malondialdehyde (MDA) and protein carbonyl in serum, advanced oxidation protein products (AOPPs) in liver and protein carbonyl in jejunal mucosa as compared with that of the CON (p<0.05). Additionally, broilers receiving HSPIO showed decreased glutathione peroxidase activity (GSH-Px) in serum, GSH and hydroxyl radical scavenging capacity in liver, GSH-Px activity in duodenal mucosa, GSH-Px activity and superoxide anion radical scavenging capacity in jejunal mucosa and increased AOPPs in serum, MDA and protein carbonyl in liver, MDA and AOPPs in jejunal mucosa (p<0.05). Conclusion: Protein oxidation of SPI can be induced by heat and soybean oil and oxidized protein resulted in redox imbalance in broilers at an early age.

Uniform DNA Biosensors Based on Threshold Voltage of Carbon Nanotube Thin-Film Transistors

Yanyan Deng,Fang Yuan,Min ZHANG,Zigang Li,Wenyu Zhou 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2016 NANO Vol.11 No.5

Uniformity is a key parameter to assure the accuracy of biosensor devices. In this work, highly uniform carbon nanotube thin-film transistors (CNT-TFTs) with a standard deviation of threshold voltage (Vth) as small as 0.04 were achieved by accurately controlling the fabrication process, which is so far the most stable distribution to our knowledge. On-state current (Ion), off-state current and on/off current ratio also exhibit high uniformity with low standard deviation of 0.50, 0.72 and 0.54, respectively. Given the high uniformity, high stability and high sensitivity, the CNT-TFTs are used as ultra-sensitive 5-hydroxymethyl cytosine (5-hmC) detecting devices for the first time, which is one of the important modified bases in DNA and plays an important role in epigenetics. After attachment of 5-hmC DNA, a reproducible and stable shift of 18.7–59% in Vth as well as a 31–54% change in Ion were observed in the transfer characteristics curves of CNT-TFTs. Thus, a detecting device of 5-hmC in DNA segments could be designed based on the highly uniform CNT-TFTs.

MAPK4 silencing in gastric cancer drives liver metastasis by positive feedback between cancer cells and macrophages

Li Shuang,Guo Dongyang,Sun Qiang,Zhang Lu,Cui Yun,Liu Min,Ma Xixi,Liu Yiman,Cui Wenyu,Sun Leimin,Teng Lisong,Wang Liangjing,Lin Aifu,Liu Wei,Zhuo Wei,Zhou Tianhua 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-

Liver metastasis is a major cause of death in gastric cancer patients, but the underlying mechanisms are poorly understood. Through a combination of in vivo screening and transcriptome profiling followed by quantitative RT-PCR and tissue array analyses, we found that mitogen-activated protein kinase 4 (MAPK4) downregulation in gastric cancer tissues from patients is significantly associated with liver metastasis and poor prognosis. The knockdown of MAPK4 in gastric cancer cells promotes liver metastasis in orthotopic mouse models. MAPK4 depletion in gastric cancer cells induces the secretion of macrophage migration inhibitory factor (MIF) to polarize tumor-associated macrophages (TAMs) in orthotopic xenograft tumors. Moreover, TAMs activate epithelial–mesenchymal transition of gastric cancer cells to suppress MAPK4 expression, which further increases MIF secretion to polarize TAMs. Taken together, our results suggest a previously undescribed positive feedback loop between cancer cells and macrophages mediated by MAPK4 silencing that facilitates gastric cancer liver metastasis.