Nutlin-3 induces HO-1 expression by activating JNK in a transcription-independent manner of p53

CHOE, YUN-JEONG,LEE, SUN-YOUNG,KO, KYUNG WON,SHIN, SEOK JOON,KIM, HO-SHIK Spandidos Publications 2014 International journal of oncology Vol.44 No.3

A recent study reported that p53 can induce HO-1 by directly binding to the putative p53 responsive element in the HO-1 promoter. In this study, we report that nutlin-3, a small molecule antagonist of HDM2, induces the transcription of HO-1 in a transcription-independent manner of p53. Nutlin-3 induced HO-1 expression at the level of transcription in human cancer cells such as U2OS and RKO cells. This induction of HO-1 did not occur in SAOS cells in which p53 was mutated and was prevented by knocking down the p53 protein using p53 siRNA transfection, but not by PFT-alpha, an inhibitor of the transcriptional activity of p53. Accompanying HO-1 expression, nutlin-3 stimulated the accumulation of ROS and the phosphorylation of MAPKs such as JNK, p38 MAPK and ERK1/2. Nutlin-3-induced HO-1 expression was suppressed by TEMPO, a ROS scavenger, and chemical inhibitors of JNK and p38 MAPK but not ERK1/2. In addition, nutlin-3-induced phosphorylation of JNK but not p38 MAPK was inhibited by TEMPO. Notably, the levels of nutlin-3-induced ROS were correlated with the mitochondrial translocation of p53 and this induction was prevented by PFT-beta, an inhibitor of the mitochondrial translocation of p53. Consistent with the effect of the ROS scavenger and MAPK inhibitors, PFT-beta reduced HO-1 expression and the phosphorylation of JNK induced by nutlin-3. In the experiments of analyzing cell death, the knockdown of HO-1 augmented nutlin-3-induced apoptosis. Collectively, these results suggest that nutlin-3 induces HO-1 expression via the activation of both JNK which is dependent on ROS generated by p53 translocated to the mitochondria and p38 MAPK which appears to be stimulated by a ROS-independent mechanism, and this HO-1 induction may inhibit nutlin-3-induced apoptosis, constituting a negative feedback loop of p53-induced apoptosis.

Antioxidant and hepatoprotective effects of Korean ginseng extract GS-KG9 in a D-galactosamine-induced liver damage animal model

Yun Ho Jo,Hwan Lee,Myeong Hwan Oh,Gyeong Hee Lee,You Jin Lee,Ji Sun Lee,Min Jung Kim,Won Yong Kim,Jin Seong Kim,Dae Seok Yoo,Sang Won Cho,Seon Woo Cha,Mi Kyung Pyo 한국영양학회 2020 Nutrition Research and Practice Vol.14 No.4

BACKGROUND/OBJECTIVES: This study was designed to investigate the improvement effect of white ginseng extract (GS-KG9) on D-galactosamine (Ga1N)-induced oxidative stress and liver injury. SUBJECTS/METHODS: Sixty Sprague-Dawley rats were divided into 6 groups. Rats were orally administrated with GS-KG9 (300, 500, or 700 mg/kg) or silymarin (25 mg/kg) for 2 weeks. The rats of the GS-KG9- and silymarin-treated groups and a control group were then intraperitoneally injected Ga1N at a concentration of 650 mg/kg for 4 days. To investigate the protective effect of GS-KG9 against GalN-induced liver injury, blood liver function indicators, anti-oxidative stress indicators, and histopathological features were analyzed. RESULTS: Serum biochemical analysis indicated that GS-KG9 ameliorated the elevation of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), and lactate dehydrogenase (LDH) in GalN-treated rats. The hepatoprotective effects of GS-KG9 involved enhancing components of the hepatic antioxidant defense system, including glutathione, glutathione peroxidase (GPx), superoxide dismutase (SOD), and catalase (CAT). In addition, GS-KG9 treatment inhibited reactive oxygen species (ROS) production induced by GalN treatment in hepatocytes and significantly increased the expression levels of nuclear factor erythroid-2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) proteins, which are antioxidant proteins. In particular, by histological analyses bases on hematoxylin and eosin, Masson"s trichrome, α-smooth muscle actin, and transforming growth factor-β1 staining, we determined that the administration of 500 mg/kg GS-KG9 inhibited hepatic inflammation and fibrosis due to the excessive accumulation of collagen. CONCLUSIONS: These findings demonstrate that GS-KG9 improves GalN-induced liver inflammation, necrosis, and fibrosis by attenuating oxidative stress. Therefore, GS-KG9 may be considered a useful candidate in the development of a natural preventive agent against liver injury.

심근 허혈-재관류 백서 모델에서 mitogen-activated protein kinase 활성화에 대한 α-lipoic acid의 효과

김연경,박은미,최준호,윤경호,유남진,이은미,김남호,정진원,김민선,박병림,오석규 圓光大學校 醫科學硏究所 2006 圓光醫科學 Vol.21 No.1

급성 뇌경색 환자에서 경색 위치가 심근 효소치 상승에 미치는 영향

윤경호,박현영,박성욱,장혁,김요식,조광호,유남진,김남호,오석규,정진원 圓光大學校 醫科學硏究所 2003 圓光醫科學 Vol.18 No.2

수냉쿨러의 냉각 효율 향상을 위한 플레이트 부품의 형상에 관한 연구

성윤호,김형찬,김선민,윤석영,이영웅,이상욱 순천향대학교 부설 산업기술연구소 2021 순천향 산업기술연구소논문집 Vol.27 No.2

This study investigates changes in cooling efficiency for heat sink plate width, perforated width, and chamfer angle as part of a parameter study to maximize cooling efficiency of heat sink by setting various variables on plates. The width of the plate is set to 16, 20, 24, 28 mm, perforated width 1.5, 2.0, 2.5 mm, chamfer angles 5, 10, 15 and 20°. By conducting a computational study with Ansys Fluent, the sink plate with plate width of 16mm, perforated width of 3mm and chamfer angle of 10° is shown to be the best cooling efficiency.

고혈압 환자들의 건강행태

김석범,강복수,윤성호,황정희,이경수 韓國保健敎育.健康增進學會 2000 보건교육건강증진학회지 Vol.17 No.1

This study was conducted to examine health behaviors in hypertensive patients and the factors that would affect these healthy life-styles, and to provide basic data for effective and continuous hypertension control in private medical facilities. Among those patients diagnosed as hypertension who had visited the Department of Internal Medicine of Yeungnam University Hospital during the two month period from August 2 to September 30, 1999, the present study included 222 patients who were in more than one month after the initial diagnosis of hypertension and those who had hypertension for less than 10 years. Using a structured questionnaire, the present study was conducted by a self-administered survey method, and the obtained data were analyzed with t-test, χ2-test and one-way analysis of variance using the SPSS statistical program. The scores on knowledge related to hypertension were higher as the education level of a patient was higher(p〈0.05). As for occupation, those who performed office or management jobs showed higher scores than those who were into manufacturing jobs, and as for economic status, although those patients who were in middle class showed highest scores, however, no statistical significance was observed. The scores of knowledge on hypertension was higher when the family history hypertension was present(p〈0.05). The scores of health-related behaviors were higher with higher education level and higher economic status, higher in those with family history of hypertension, and higher in those with office or management jobs than those who had manufacturing jobs(p〈0.05). Blood pressure measurement on regular basis was performed most frequently in those who were between 50∼59 years old with 83.3% and was least frequent in those who were older than 70 years old with 50%(p〈0.05). The frequency of regular blood pressure measurement was higher with higher education level, higher economic status, higher in those with family history of hypertension, and the highest in those with management position with 93.5%, however, on statistical significance was observed. Changes in health-relaxed behaviors after hypertension diagnosis were higher with higher education level, higher economic status, and in those patients performed office or management work. In particular, diet change was observed in female and higher economic status and smoking cessation was observed in 60∼69 years old. Housewives and office workers or managers have taken exercise more regularly and those who had management jobs and had high scores on knowledge related to hypertension would participate health education program more actively. Thus, for improving health-related behaviors for continuous management of hypertension, changes in health-related behaviors can be followed through conduction health education to improve understanding of knowledge related to hypertension as the method of helping to improve changes in health life-styles in those with little education and those in low economic status.

器內培養에 의한 Denphalae의 大量增殖

강호종,박상현,채윤석 진주산업대학교 1992 論文集 Vol.31 No.-

Denphalae(Dendrobium phalaenopsis)의 대량증식에 관한 실험을 실시한 결과를 요약하면 다음과 같다. 1. 식물재료의 소독은 sodium Hypochlorite에 먼저 씻은 후 Ethylalcohol 70%에 l0초, Ca(CIO)_2 5%에 5분, 껍질 제거후 1% Ca(ClO)_2에 10분, 그후 미세조정후 치상하는 것이 가장 양호하였다. 2. MS기본배지에 Sucrose 3%, NAA O.5, BA O.5ppm, 사과즙액 10%, 감자즙액 10%, 참가한 pH 5.4의 액체진탕에서 가장 증식이 양호하였고 진탕속도는 30~40rpm/min이 좋았다. 3. 배양환경의 적용범위는 대체로 넓었으며 온도는 24~28℃, 일장은 8~l6시간, 조도는 1,000∼3,000Lux에서 별 차이를 보이지 않았으나 온도는 26℃土1℃, 일장은 l6시간, 조도는 l,500Lux로 조정하여 배양한 결과는 배우 양호 하였다. Effects of Sterilization on the Shoot-tip and medium, Temperature, Vibration and Photolength on the growth of Denphalae were studied using tissue cultural method 1. It is evident that the treatment of 1% calsium hypoclorne for 10 minutes after taking off the outer covers, 5% Ca(C10)_2 for 5 minuter and 70% ethyl alcohol for l0 seconds on the shoot-tip is an useful technique in sterilizaton. 2. The medium with the addition of 3% sucrose, 0.5ppm NAA, 0.5ppm BA, l0% apple juice, and 10% potato extract in pH 5.4 under the vibration speed of 30 to 40 rpm for 1 minuter showed a good effect on the growth of Denphalae. 3. The optimum range of the growth temperature, day lenth and photoperiod for Denphalae found to be 24 to 28℃, 8 to 16 hours and l,000 lux respectively. The enviromental condition of temperatue, day length and photoperiod for mass production of Denphalae showed 26^± 1℃ 16 hours and 1,500 lux respectivery.

삽목방법이 묘소질과 토마토 생육에 미치는 영향

채윤석,박상대,강호종 진주산업대학교 농업기술연구소 2007 農業技術硏究所報 Vol.20 No.-

본 시험은 60일 된 토마토 실생묘의 정단부를 제외하고 상위, 중위, 하위 각 2마디를 하나로, 푸르미, 토실이 용토에 마디삽목하여 묘의 소질을 조사하고, 육성된 마디삽목묘를 저단밀식 재배하여 토마토의 생육과 과실의 특성에 미치는 영향을 살펴본 결과는 다음과 같다 1. 삽목 10일과 30일 후 신초장 및 신초경의 생육은 하나로, 푸르미용토가 유의적인 차이가 있었으며, 20일 후 신초장과 신근은 푸르미용토에서 삽수채취 하위부분에서 길었다. 2. 최종수확기에서 잎수는 푸르미상토가 많았고, 마디수는 중·하위절이 많았다. 초장과 엽장, 지상부 및 지하부 생체중과 건물중은처리간 유의성이 없었다. 3. 생육중기의 엽록소함량은 푸르미용토와 중·상위 절이 많았으나 최종수확기 무렵에는 처리구간 비슷하였다. This study was conducted to investigate the effects of the bedsoils(H,P and T) and the cutting sites the seedling quality, growth and fruit characters in the dense planting culture of tomato. The samples for test were prepared by dividing three parts(upper, middle and lower part) of 60days old healthy seedlings of tomatoes and collecting 2 modes from each part except the shoot position. The tomato's major characters, seedling quality, fruit character and soluble solids content were measured after 10, 20, and 30 days. 1. The new shoot length grew up bed soil of Hanaro, Promi but Hanaro soil thicken new shoot diameter and new root grew highly of promi soil, lower node in the 10 days after cutting. The growth of new shoot length in the 20 days after cutting grew up highly Promi soil and upper position node, middle position node, the growth of new root grew up highly Promi soil, middle position node and lower node. The growth of new shoot length in the 30 days after cutting grew up highly Hanaro soil and new root number was good enough in Hanaro soil and upper position, middle position node. 2. Leaf number was a lot in Promi soil at harvest time but node number was a lot in middle and lower position node. Flesh and dry weight of plant heigh, leaf, stem and root was similar. 3. The chlorophyll content of growing period on 20 May and 20 June was high in Promi soil and middle, lower position node but on 20 July was similar to all treatments.

크론병에 대한 CoPPLX의 방어효과에 대한 연구

이호학,김유림,문창기,이재일,양병윤,김종수,이정민,정재혁,안석진,박상준,김윤권,김소연,김영중,조민구,최영자,장성조 圓光大學校 醫科學硏究所 2005 圓光醫科學 Vol.20 No.1

여름 고추육묘의 트라이졸계 처리에 의해 유기된 생육억제 해소에 관한 연구

채윤석,강호종,황서진 진주산업대학교 농업기술연구소 2007 農業技術硏究所報 Vol.20 No.-

고온기 고추육묘에 처리한 생장억제제 처리 효과 및 정식 후 생육억제를 회복하기 위하여 GA, 관수, 고온처리 효과에 대한 연구 결과는 다음과 같다. 1.생장억제제 트리아졸계 처리 횟수에 따라 초장 및 주경장은 뚜렷한 차이를 나타냈고 근장도 2회 처리를 함으로 1회 처리를 한 것보다 발달하지 않았다. 2. 왜화된 고추묘의 생육억제 해소에 미치는 영향은 초장 및 주경장의 생장에는 GA처리구가 뚜렷하였으나 다른 처리구는 비슷한 경향이었고 경경은 변화가 없었다. 3. 생육회복을 위한 처리별 생체 및 건물중은 정식 후 30일까지는 변화가 거의 없었으나 그 후 45일까지 급격히 증가하였는데 고온 및 관수처리구에서 뚜렷한 차이를 보였으며 45일 이후에는 모든 처리구에서 비슷한 경향을 보였다. 4. 정식 45일 경 처리구간 엽록소 함량은 GA처리구에서 가장 낮았으며 다른 처리구간에는 비슷했다. This study was carried out to find effects of restoration of hot pepper seedling dwarfed by the treatment of diniconazole by introducing GA₃, Irrigation and high temperature at knight and the results obtained are summarized as follows. The main results of this research are summarized as follows. 1. In the case of plant height and main stem hight treated with the first time and second time diniconazole showed the strongest effect than untreatment. The root length treated with diniconazole the first time effect gradually disappeared than the second time. 2. Sprays of GA₃100ppm to the dwarfed seedlings by diniconazole pretreatment were effective in counteracting the plant height and main stem height growth inhibition caused by diniconazole pretreatment. 3. The fresh and dry weight of pepper seedling were not significantly different untill 30days in planting, but were increased to 45days from 30days in planting. 4. The chlorophyll content were decreased more or less treatment of GA₃ 100ppm after 45days in planting.