The Determinants of Self-Management in Patients with Diabetic Nephropathy under the eHealth care Era

Yu-Shan Huang,Chia-En Li,Yu-Chi Chen 한국간호과학회 2023 한국간호과학회 학술대회 Vol.2023 No.11

Quantitative Computed Tomography of Pulmonary Emphysema and Ventricular Function in Chronic Obstructive Pulmonary Disease Patients with Pulmonary Hypertension

Yu-Sen Huang,Hsao-Hsun Hsu,Jo-Yu Chen,Mei-Hwa Tai,Fu-Shan Jaw,Yeun-Chung Chang 대한영상의학회 2014 Korean Journal of Radiology Vol.15 No.6

Objective: This study strived to evaluate the relationship between degree of pulmonary emphysema and cardiac ventricular function in chronic obstructive pulmonary disease (COPD) patients with pulmonary hypertension (PH) using electrocardiographic-gated multidetector computed tomography (CT). Materials and Methods: Lung transplantation candidates with the diagnosis of COPD and PH were chosen for the study population, and a total of 15 patients were included. The extent of emphysema is defined as the percentage of voxels below -910 Hounsfield units in the lung windows in whole lung CT without intravenous contrast. Heart function parameters were measured by electrocardiographic-gated CT angiography. Linear regression analysis was conducted to examine the associations between percent emphysema and heart function indicators. Results: Significant correlations were found between percent emphysema and right ventricular (RV) measurements, including RV end-diastolic volume (R2 = 0.340, p = 0.023), RV stroke volume (R2 = 0.406, p = 0.011), and RV cardiac output (R2 = 0.382, p = 0.014); the correlations between percent emphysema and left ventricular function indicators were not observed. Conclusion: The study revealed that percent emphysema is correlated with RV dysfunction among COPD patients with PH. Based on our findings, percent emphysema can be considered for use as an indicator to predict the severity of right ventricular dysfunction among COPD patients.

Decreased Expression of LKB1 Correlates with Poor Prognosis in Hepatocellular Carcinoma Patients Undergoing Hepatectomy

Huang, Yue-Han,Chen, Zhen-Kun,Huang, Ka-Te,Li, Peng,He, Bin,Guo, Xu,Zhong, Jun-Qiao,Zhang, Qi-Yu,Shi, Hong-Qi,Song, Qi-Tong,Yu, Zheng-Ping,Shan, Yun-Feng Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.3

Aim: To study any correlation of LKB1 expression with prognosis in hepatocellular carcinoma (HCC) cases. Methods: A total of 70 HCC patients and 20 primary intrahepatic stone patients in the first affiliated hospital of Wenzhou Medical College were enrolled in this study. LKB1 expression was detected by immunohistochemistry. Patients were followed-up and prognostic factors were evaluated. Result: LKB1 expression was decreased in the HCC samples. Loss of LKB1 expression in HCC was significantly related to histologic grade (P=0.010), vascular invasion (P=0.025) and TMN stage (P=0.011). Patients showing negative LKB1 expression had a significantly shorter disease-free and overall survival than those with positive expression (P = 0.001, P=0.000, respectively). Multivariate Cox regression analysis indicated that LKB1 expression level was an independent factor of survival (P = 0.033). Conclusion: HCC patients with decreased expression LKB1 have a poor prognosis. The loss of LKB1 expression is correlated with a lower survival rate.

Using the Juice of Water Lettuce (Pistia stratiotes) as Culture Medium to Increase the Cell Density and the Production of Microbial Lipid

Yu-Hsien Lien,Fu-Yao Liu,Jyy-Ning Chen,Yu-Shan Huang,Yu-Hong Wei,Chiyang Yu,Che-Chi Shu 한국생물공학회 2019 Biotechnology and Bioprocess Engineering Vol.24 No.2

Water lettuce (Pistia stratiotes) is one of the most well-known aquatic weeds as it causes problems in watercourses all over the world. This invasive species is fast-growing and thus has the potential for its use in preparing growth medium of microorganisms. Toward it, the pretreatment and enzymatic saccharification are positively the decisive processes. But there are other crucial processes, which are usually ignored by researchers. To the best of my knowledge, this presented work is the first time discovering that the juice obtained from water lettuce is valuable. Except for sterilization, no treatment is needed for the water lettuce’s juice and it can be directly served for cell growth. For Escherichia coli, Saccharomyces cerevisiae, Pichia pastoris, Bacillus subtilis, Lactococcus Lactis, Lactobacillus rhamnosus, and Lactobacillus plantarum, the cell density in the broth of water lettuce’s juice as the only carbon source is 10-60% higher than that in LB, YPD, BHI, M17, MRS. We then examined the production of microbial lipid by YM prepared in the juice of water lettuce. In comparison to commercial medium YM broth, the YM with juice caused 84% increase in the production of microbial lipid. A simple process of collecting juice notably increased productivity.

중국 고대 사(詞)와 조선 고전 시조(時調)의 아속(雅俗) 특징 비교 연구

황현옥 ( Huang Xian-yu ),우상열 ( Yu Shan-glie ) 한국시가문화학회(구 한국고시가문화학회) 2017 한국시가문화연구 Vol.0 No.40

詞란 당나라 시기 민간에서 발생하고 송대에 이르러 점차 대중화된 문학 장르이다. 중국의 詞와 조선의 時調는 고대 중국과 조선의 주요한 시가형식의 하나로서 많은 可比性을 가지고 있다. 하지만 많은 학자들은 중국 고대사가 조선에 미친 영향에 주의를 돌리고 많은 면에서 연구를 진행하였다. 조선의 지정학적 조건으로 한사군 시대나 더 이전 시대부터 중국의 선진문화를 받아들여 조선의 문물제도나 규범으로 사용한 것은 다 알고 있는 사실이다. 특히 신라시대에 들어서면서 당나라와의 외교가 밀접해지고 당의 문물과 서역문화를 받아들여 이후의 불교미술과 향가문학을 꽃 피우는데 유리한 조건을 마련해 주었으며 당으로 유학을 가는 사람들도 나타났다. 고려로 들어서면서 중국을 학습하는 추세는 더욱 강화되었고 惟我東方, 舊慕唐風, 文物禮樂, 犧尊其制라는 방침까지 나오게 되었다. 그 후 고려 중기부터 송나라와의 관계가 끊임없이 밀착되면서 신라와 고려 전기에 당시를 존숭하던 기풍이 점차적으로 변하면서 蘇軾의 시가 전범으로 유행하게 되었다. 물론 많은 사람들은 蘇軾의 시문을 칭송하지만 그의 詞도 높이 평가하고 있다. 하지만 가령 蘇軾의 영향을 받았다 하더라도 단지 영향이 미쳤다는 가정에서 논술이 끝나면 안 된다. 특히 현재까지 진행된 연구들을 보면 詞와 時調가 단지 간단한 영향관계만 가지게 된 것이 아니다. 조선의 時調는 일찍 13세기부터 등장하였는데 그 당시는 “短歌”라고 불렸고 時調라고 불리기 시작한 것은 18세기부터이다. 하지만 13세기에 등장한 時調의 전신인 “短歌”는 특징상 많은 면에서 중국의 詞와 비슷한 점들을 지니고 있다. 예를 들어 詞와 時調는 모두 그 “詩餘”에서 정통적인 지위를 가지지 않았다. 이러한 특징은 유형학상의 비교가능성을 보여주고 있다. 또 최초 詞의 주류는 기존의 염정과 이별의 한을 한을 노래한 사를 이었으며 時調의 창작 층에는 기녀들도 있었다. 이처럼 詞와 時調는 모두 민간에서 기원되었다. 이외 柳永과 같은 저명한 詞人들은 하층민들의 생활에 접근하여 민간에서 사용하는 생동감 있는 구어들을 많이 사용 하였다. 그 후 蘇軾 등 많은 사람들도 詞에 대해 혁신을 진행하였으나 이속적인 요소들은 개변되지 않았다. 이러한 원인으로 하여 기타 시에서는 볼 수 없는 통속적인 특점을 가지게 되었다. 위에서 말한 것과 같이 時調가 탄생하게 된 계기로 詞의 영향을 빼놓을 수 없기에 영향연구가 진행될 수 있는 것은 당연한 일이다. 하지만 時調의 성숙단계에 들어서면서 많은 하층인민들이 창작에 뛰어들었는데 이는 또 詞의 영향에서 벗어난 時調가 탄생되도록 조건을 마련해주었다. 이러한 점들을 근거로 하여 본 논문은 중국의 詞와 조선 時調에 대해 종합비교연구를 진행하였다. This paper is a comprehensive comparison of Chinese Ci and Korean Shijo, which have a strong comparability as one of the main poetic carriers of ancient China and Korea. Occupying no orthodox literary status, Ci and Shijo are called the "remainder of poetry", which also highlights their typological value and comparative possibility. In addition, Ci and Shijo are originated in the folk. Liu Yong and other famous poets, who were familiar with the living of working class people, used a lot of vernacular language. Later, an innovation was advocated by Su Shi and others, but the nonorthodox heterogeneous elements of Ci and Shijo are still vigorous. Therefore, Ci and Shijo save the vulgar features which are rare in other forms of the poetry.

The Effective Program of Cardio Pulmonary Resuscitation in Parents with Preterm Infants

Clagn Yu Shan,Chen Ching Ju,Chiu Ya Hui,Lin Ruei Yan,Lee Jo Hua,Huang Li Chi 한국간호과학회 2011 한국간호과학회 학술대회 Vol.2011 No.10

Identification and Validation of Circulating MicroRNA Signatures for Breast Cancer Early Detection Based on Large Scale Tissue-Derived Data

Xiaokang Yu,Jinsheng Liang,Jiarui Xu,Xingsong Li,Shan Xing,Huilan Liu,Wan-Li Liu,Dongdong Liu,Jianhua Xu,Lizhen Huang,Hongli Du 한국유방암학회 2018 Journal of breast cancer Vol.21 No.4

Purpose: Breast cancer is the most commonly occurring cancer among women worldwide, and therefore, improved approaches for its early detection are urgently needed. As microRNAs (miRNAs) are increasingly recognized as critical regulators in tumorigenesis and possess excellent stability in plasma, this study focused on using miRNAs to develop a method for identifying noninvasive biomarkers. Methods: To discover critical candidates, differential expression analysis was performed on tissue-originated miRNA profiles of 409 early breast cancer patients and 87 healthy controls from The Cancer Genome Atlas database. We selected candidates from the differentially expressed miRNAs and then evaluated every possible molecular signature formed by the candidates. The best signature was validated in independent serum samples from 113 early breast cancer patients and 47 healthy controls using reverse transcription quantitative real-time polymerase chain reaction. Results: The miRNA candidates in our method were revealed to be associated with breast cancer according to previous studies and showed potential as useful biomarkers. When validated in independent serum samples, the area under curve of the final miRNA signature (miR-21-3p, miR-21-5p, and miR-99a-5p) was 0.895. Diagnostic sensitivity and specificity were 97.9% and 73.5%, respectively. Conclusion: The present study established a novel and effective method to identify biomarkers for early breast cancer. And the method, is also suitable for other cancer types. Furthermore, a combination of three miRNAs was identified as a prospective biomarker for breast cancer early detection.

Mesenchymal Stem Cell Secreted-Extracellular Vesicles are Involved in Chondrocyte Production and Reduce Adipogenesis during Stem Cell Differentiation

Tsai Yu-Chen,Cheng Tai-Shan,Liao Hsiu-Jung,Chuang Ming-Hsi,Chen Hui-Ting,Chen Chun-Hung,Zhang Kai-Ling,Chang Chih-Hung,Lin Po-Cheng,Huang Chi-Ying F. 한국조직공학과 재생의학회 2022 조직공학과 재생의학 Vol.19 No.6

BACKGROUND: Extracellular vesicles (EVs) are derived from internal cellular compartments, and have potential as a diagnostic and therapeutic tool in degenerative disease associated with aging. Mesenchymal stem cells (MSCs) have become a promising tool for functional EVs production. This study investigated the efficacy of EVs and its effect on differentiation capacity. METHODS: The characteristics of MSCs were evaluated by flow cytometry and stem cell differentiation analysis, and a production mode of functional EVs was scaled from MSCs. The concentration and size of EVs were quantitated by Nanoparticle Tracking Analysis (NTA). Western blot analysis was used to assess the protein expression of exosomespecific markers. The effects of MSC-derived EVs were assessed by chondrogenic and adipogenic differentiation analyses and histological observation. RESULTS: The range of the particle size of adipose-derived stem cells (ADSCs)- and Wharton’s jelly -MSCs-derived EVs were from 130 to 150 nm as measured by NTA, which showed positive expression of exosomal markers. The chondrogenic induction ability was weakened in the absence of EVs in vitro. Interestingly, after EV administration, type II collagen, a major component in the cartilage extracellular matrix, was upregulated compared to the EV-free condition. Moreover, EVs decreased the lipid accumulation rate during adipogenic induction. CONCLUSION: The results indicated that the production model could facilitate production of effective EVs and further demonstrated the role of MSC-derived EVs in cell differentiation. MSC-derived EVs could be successfully used in cell-free therapy to guide chondrogenic differentiation of ADSC for future clinical applications in cartilage regeneration.

Identification of flight task using ensemble empirical mode decomposition based analysis method

Biting Yu,Bo Jia,Qi Wu,Yanyu Lu,Dan Huang,Shan Fu 제어로봇시스템학회 2015 제어로봇시스템학회 국제학술대회 논문집 Vol.2015 No.10

To analyse the difficulty of flight tasks, a novel method was presented in this paper using pupil diameter of pilots. The proposed method integrated ensemble empirical mode decomposition (EEMD) and ratio of high to low frequency wavelet coefficients energy to identify pupil data under different tasks from 5 crews. Compared to the extraction method using wavelet energy entropy, the proposed method got more obvious difference between manual departure and standard instrument departure. The results indicated that the difficulty of flight tasks could be distinguished by pupil diameter of pilots.

Role of IL-18 Gene Promoter Polymorphisms, Serum IL-18 Levels, and Risk of Hepatitis B Virus-related Liver Disease in the Guangxi Zhuang Population: a Retrospective Case-Control Study

Lu, Yu,Bao, Jin-Gui,Deng, Yan,Rong, Cheng-Zhi,Liu, Yan-Qiong,Huang, Xiu-Li,Song, Liu-Ying,Li, Shan,Qin, Xue Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.14

Background: The aim of this study was to assess the relationship between IL-18 gene polymorphisms and HBV-related diseases and whether these polymorphisms influence its expression in the Guangxi Zhuang population. Materials and Methods: We enrolled 129 chronic HBV infected (CHB) patients, 86 HBV-related liver cirrhosis (LC) patients and 160 healthy controls in our study. Polymerase chain reaction-restriction fragment length polymorphism methods were used to detect IL-18 gene -607C/A, -137G/C polymorphisms, and an ELISA kit was employed to determine serum IL-18 levels. Results: No correlation was found between the -607C/A polymorphism and risk of HBV-related disease. For the -137G/C polymorphism, the GC genotype and C allele were associated with a significantly lower risk of CHB (95%CI: 0.32-0.95, p=0.034 and 95%CI: 0.35-0.91, p=0.018) and HBV-related LC (95%CI: 0.24-0.89, p=0.022 and 95%CI: 0.28-0.90, p=0.021). A similar decreased risk was also found with the A-607C-137 haplotype. With respect to IL-18 expression, it was significantly lower in both patient groups, but no association was noted between the two polymorphisms in the IL-18 gene and its expression. Conclusions: Our study indicated that the -137C allele in the IL-18 gene may be a protective factor for HBV-related disease, and serum IL-18 level may be inversely associated with CHB and HBV-related LC.