환경경영시스템의 효과 제고를 위한 환경측면 파악방법 연구

정유심,이상용,김회배 한국품질경영학회 2000 품질혁신 Vol.1 No.1

96년 ISO 14001 국제규격의 개발과 동시에 우리나라에서도 본격적인 환경경영시스템 인증제도가 시작되어, 기업경영에서 환경문제에 대한 적극적인 고려가 가시화되기 시작했다. 한편, 최근 사회적으로 환경에 대한 인식이 증가하면서 환경경영시스템 도입을 통해 이러한 인식에 부응하여 다양한 경영성과를 기대하는 조직의 요구도 커지고 있는 상황이다. 조직의 관점에서 경영성과란 결국 조직의 수익창출에 기여할 수 있는 성과를 거두는 것이기 때문에 환경경영시스템이 추구하는 목적은 환경영향의 저감을 통해 이러한 효과를 달성코자 하는 것이다. 이와 관련하여 본 연구에서는 조직이 발생시키는 환경영향의 요인, 즉 환경측면과 그 파악방법에 대한 이해를 통해 환경경영시스템의 효과 제고를 모색코자 한다.

경막 내 colistimethate 투여로 치료한 다제 내성 cinetobacter baumannii 뇌실염

홍유아,유진홍,김진진,모은영,안건희,정희경,김진석,이현정,정미향,윤승배 대한감염학회 2009 감염과 화학요법 Vol.41 No.4

Acinetobacter species is a non-fermentative aerobic gram-negative coccobacillus that is an important pathogen found in nosocomial infections. Recently, multi-drug resistant Acinetobacter baumannii (MDR-AB) infections have been increasing and pose a serious problem. Most such infections present as bacteremia, pneumonia, or a wound infection; however, CNS infections are very rare. We herein present a case of ventriculitis caused by MDR-AB in a 37-year old man after a neurosurgical intervention. The patient was successfully treated with intrathecal colistimethate.

국내산 과일 및 채소 추출물을 이용한 체중 조절 관련 기능성 식품소재 탐색

김인혜;김종배;조강진;황유진;황경아;고정숙;엄애선 한양대학교 2011 韓國 生活 科學 硏究 Vol.31 No.1

Obesity can be defined as a disorder of lipid metabolism and the control of this process is regulated by enzymes involved in lipid metabolic pathway. Obesity is also characterized increases in the number and size of adipocyte differentiated from fibroblastic pre-adipocytes in adipose tissue. The aim of this study was to screen ethanol extracts of 21 fruits and vegetables cultivated in Republic of Korea for identifying natural sources of lipase inhibitor and to investigate the effects of several extracts resulting in suppression of triglyceride (TG) accumulation in 3T3-L1 cells. Among the tested extracts, Diospyros kaki L. showed the highest contents of total sugar and the content of total phenolic compounds was detected 17 species while there was not detected in Ixeris dentate root and Allium fistulosum C. etc. We were screened 26 extracts of fruits and vegetables for pancreatic lipase inhibitory activity compared with orlistat. The strongest lipase inhibitory activities were shown by extract of Allium sativum L. stem, Allium sativum L. bulb, Apteryx australis, Diospyros kaki L. Malus Pumila, Perilla frutescens and Prunus mume regardless of concentrations treated. Eight extracts including Brassica juncea var. integrilolia had no effect on lipase inhibition. During the differentiation of 3T3-L1 preadipocyte to mature adipocyte, the cells were treated with carrier or 7 extracts(1, 5 and 10 ㎍/ml), which was shown to highest lipase inhibitory effects. We were quantified lipid contents secreted mature adipocytes and stained with Oil Red O. All cells treated with the Apteryx australis and Prunus mume significantly decreased TG contents than the control. These findings suggested that the screened fruit and vegetable could be a promising source as the effective and safe lipase inhibitors for isolating bioactive ingredients and developing functional foods.

분만 후 심한 저혈당이 동반된 림프구성 뇌하수체염 1예의 경과 관찰

손장명,고승현,안유배,주강,이정록,양성은,송기호,손호영,전정수 대한내분비학회 2003 Endocrinology and metabolism Vol.18 No.3

저자들은 출산 후 발생한 심한 저혈당을 주소로 내원한 31세 여자 환자에서 뇌 자기공명영상검사에서 뇌하수체종괴가 관찰되고 복합 뇌하수체 기능검사 결과전반적인 뇌하수체 기능저하증의 소견을 보여 림프구성 뇌하수체염을 진단하고, 호르몬 보충요법만으로 경과를 관찰하여 호전된 예를 경험하였기에 문헌 고찰과 함께 보고하는 바이다. Lymphocytic hypophysitis, an inflammatory disease of the pituitary gland that is often associated with pregnancy, is caused by autoimmune destruction of the pituitary gland evidenced by diffuse inflammatory cell infiltration of the pituitary gland and some kinds of detectable autoantibodies. We report a case of lymphocytic hypophysitis in a 31-year-old woman presenting with severe hypoglycemia and hyponatremia after delivery. Hormonal study revealed panhypopituitarism and magnetic resonance imaging with enhancement showed the bulging contour of the right side pituitary gland with an ill-defined mass-like lesion and nodular thickening of the stalk The patient's symptoms and biochemical data improved greatly with replacement of L-thyroxine and glucocorticoid. Partial recovery of panhypopituitarism was also seen. The follow-up tests revealed dramatic resolution of the pituitary lesion (J Kor SOC Endocrinol 1 8 : 3 25 -33 1, 2003).

Effect of addition of methanol on rheological properties of silk formic acid solution

( Yu Jeong Bae ),( In Chul Um ) 한국잠사학회 2020 International Journal of Industrial Entomology Vol.40 No.1

Recently, many studies have been undertaken on the wet spinning and electrospinning of silk because wet-spun fibers and electrospun webs of silk can be applied in the biomedical and cosmetic fields owing to the good biocompatibility of silk. The rheological properties of silk solution are important because they strongly affect the spinning performance of the silk solution and the structures of resultant fibrous materials. Therefore, as a preliminary study on the effect of solvent composition on the rheological properties of silk fibroin (SF) solution and structure of the resultant film, in the reported work, methanol was added to the SF formic acid solution. A small amount of methanol (i.e. 2%) added to the SF formic acid solution significantly altered the rheological properties of the solution: its shear viscosity increased by 10 folds at low shear and decreased on increasing the shear rate, demonstrating shear thinning behavior of the SF solution. Dynamic tests for the SF solution indicated that the addition of 2% methanol altered the viscous state of the SF formic acid solution to elastic. However, the molecular conformation (i.e. β-sheet conformation) of the regenerated SF film cast from formic acid remained unchanged on the addition of 2% methanol. © 2020 The Korean Society of Sericultural Sciences Int. J. Indust. Entomol. 40(1), 28-32 (2020)

Morphology and spin transport in organic spin devices

Yu Jeong BAE,Nyun Jong LEE,Jessica WADE,Ji-Seon KIM,Eisuke ITO,Tae Hee KIM 한국진공학회 2016 한국진공학회 학술발표회초록집 Vol.2016 No.8

Alpha-lipoic Acid Protects Neuronal Cells from Ethanol-induced Neurotoxicity

Yu Jeong Bae,Yoo Hun Noh,Do Hee Kim,Jiae Park,Ok Hyeon Kim,Seung-Ah Lee,Yoon Hee Chung,Seung Ho Han,Kyung Yong Kim,Won Bok Lee 중앙대학교 의과대학 의과학연구소 2014 中央醫大誌 Vol.39 No.2

Ethanol exposure has various toxic effects on the human nervous system results in various developmental and behavioral deficits. Present studies showed the protective effect of alpha-lipoic acid (LA), a potent natural anti-oxidant and anti-inflammatory molecule against various brain toxins. Since LA has attracted more attention for its anti-apoptotic properties, we investigated the neuroprotective efficacy of LA in EtOH-treated SH-SY5Y human neuroblastoma cells. The cell death was evaluated by MTT assay, morphological assessment. EtOH induced neuronal cell death in a time- and dose-dependent manner. The cell death examined was increased and the apoptotic morphological features, including retraction of neuritis, cell shrinkage, and membrane blebbing were induced by EtOH. However, in the cells treated with LA for 1h and exposed to EtOH the EtOH-induced neuronal cell death was significantly attenuated in a dose-dependent manner. The LA effectively inhibited SH-SY5Y cell death and apoptotic morphological changes in the cells induced by EtOH. Our results indicate that pretreatment with LA interrupts EtOH-induced apoptotic morphological change, and suggest that some of the protective effects of LA and other antioxidants likely involve classical antioxidant actions by EtOH-related brain damages and nerve injuries by oxidative stress which causes apoptosis.

Metabolic Engineering for Resveratrol Derivative Biosynthesis in Escherichia coli

Jeong, Yu Jeong,Woo, Su Gyeong,An, Chul Han,Jeong, Hyung Jae,Hong, Young-Soo,Kim, Young-Min,Ryu, Young Bae,Rho, Mun-Chual,Lee, Woo Song,Kim, Cha Young Korean Society for Molecular and Cellular Biology 2015 Molecules and cells Vol.38 No.4

We previously reported that the SbROMT3syn recombinant protein catalyzes the production of the methylated resveratrol derivatives pinostilbene and pterostilbene by methylating substrate resveratrol in recombinant E. coli. To further study the production of stilbene compounds in E. coli by the expression of enzymes involved in stilbene biosynthesis, we isolated three stilbene synthase (STS) genes from rhubarb, peanut, and grape as well as two resveratrol O-methyltransferase (ROMT) genes from grape and sorghum. The ability of RpSTS to produce resveratrol in recombinant E. coli was compared with other AhSTS and VrSTS genes. Out of three STS, only AhSTS was able to produce resveratrol from p-coumaric acid. Thus, to improve the solubility of RpSTS, VrROMT, and SbROMT3 in E. coli, we synthesized the RpSTS, VrROMT and SbROMT3 genes following codon-optimization and expressed one or both genes together with the cinnamate/4-coumarate:coenzyme A ligase (CCL) gene from Streptomyces coelicolor. Our HPLC and LC-MS analyses showed that recombinant E. coli expressing both ScCCL and RpSTSsyn led to the production of resveratrol when p-coumaric acid was used as the precursor. In addition, incorporation of SbROMT3syn in recombinant E. coli cells produced resveratrol and its mono-methylated derivative, pinostilbene, as the major products from p-coumaric acid. However, very small amounts of pterostilbene were only detectable in the recombinant E. coli cells expressing the ScCCL, RpSTSsyn and SbROMT3syn genes. These results suggest that RpSTSsyn exhibits an enhanced enzyme activity to produce resveratrol and SbROMT3syn catalyzes the methylation of resveratrol to produce pinostilbene in E. coli cells.

Artificial Mutation for Silkworm Molecular Breeding Using Gene Scissors

Jeong Won Hong(홍정원),Chan Young Jeong(정찬영),Jeong Hee Yu(유정희),Su-Bae Kim(김수배),Sang Kuk Kang(강상국),Seong-Wan Kim(김성완),Nam-Suk Kim(김남숙),Kee Young Kim(김기영),Jong Woo Park(박종우) 한국생명과학회 2020 생명과학회지 Vol.30 No.8

Clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR associated protein (Cas)9을 이용하는 유전자 가위 기술은 미래 육종 기술로서 주목받고 있다. 본 연구에서는 3세대 유전자 가위 CRISPR/Cas9을 이용한 누에 kynurenine 3-monooxygenase (KMO) 유전자 편집을 통한 돌연변이 유발 및 선택 교배를 통하여 유전자의 세대간 전달을 분석하고자 하였다. 유전자 편집을 위하여 누에의 KMO 유전자에 대한 3종의 가이드 RNA를 제작하고, 제작된 gRNA는 Cas9 단백질과 복합체를 형성시켜 누에 세포주(BM-N)에 도입 후 T7 endonucleaseI 분석을 수행하여 최적의 gRNA를 선발하였다. 선발된 K1N gRNA는 누에 유전자를 편집하기 위하여 Cas9 단백질과 복합체를 형성시킨 후 누에 초가 배아에 미세주사하고 사육하였다. 미세주사 후 부화율은 18% 가량으로 낮게 나타났으나 생존한 개체 중 돌연변이 발생율은 60% 이상으로 비교적 높게 나타났다. 돌연변이가 발생된 G0세대의 KMO 유전자는 이형접합자 형태로 나타났으며, 표현형의 변화는 관찰되지 않았다. 하지만 형접합자들 사이의 근친 교배에 의해 탄생한 G1세대 돌연변이에서는 일부에서 알과 눈의 색 변화가 확인되었으며, 변이가 확인된 개체들사이의 근친교배를 통해 생산된 G2세대에서는 모든 개체에서 표현형의 변화가 나타났다. 이러한 결과에 비추어 볼 때, 유전자 가위를 이용한 돌연변이 육종에는 한계가 있으나 전통 교배 육종과 융합을 통하여 육종 기간을 비약적으로 단축시킬 수 있는 곤충 육종 기술로 발전가능성이 높다고 판단된다. Gene editing technology using the clustered regularly interspaced short palindromic repeat (CRISPR) and the CRISPR associated protein (Cas)9 has been highly anticipated in developing breeding techniques. In this study, we discuss gene scissors as a tool for silkworm molecular breeding through analysis of Bombyx mori Kynurenine 3-Monooxygenase (BmKMO) gene editing using the CRISPR/Cas9 system and analysis of generational transmission through mutagenesis and selective crossing. The nucleotide sequence of the BmKMO gene was analyzed, and three guide RNAs (gRNAs) were prepared. Each synthesized gRNA was combined with Cas9 protein and then analyzed by T7 endonuclease I after introduction into the BM-N silkworm cell line. To edit the silkworm gene, K1P gRNA and Cas9 complexes were subsequently microinjected into the silkworm embryos; the hatching rate was 18% and the incidence of mutation was 60%. The gene mutation was verified in the heterozygous G0 generation, but no phenotypic change was observed. In homozygotes generated by self-crossing, a mutant phenotype was observed. These results suggest that silkworm molecular breeding using the CRISPR/ Cas9 system is possible and could be an effective way of shortening the time required.

Adrenocortical Carcinoma Presenting as a Adrenal Incidentaloma

Yu Jin Jeong · Seok Won Kim · Hee Jeong Lee · Jin Hwa Kim · Sang Yong Kim · Hak Yeon Bae 조선대학교 의학연구원 2010 The Medical Journal of Chosun University Vol.35 No.2

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