Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

브라켓 부착시 실런트 레진이 법랑질 탈회에 미치는 영향

윤영아,이기헌,황현식,홍석진 전남대학교 치의학연구소 2002 구강과학 Vol.14 No.1

The purpose of this study was to evaluate the effects of a sealant resin on enamel demineralization in orthodontic bracket bonding. The forty eight extracted sound bovine teeth was subdivided into four groups and treated with Phase Ⅱ^(r) on the surface. Group 1 was not treated, Group 2 was acid etched with 37% phosphoric acid for 30 seconds, Group 3 was sealant applied after acid etching and Group 4 was resin paste applied after acid etching and sealant application. Each group was demineralized in artificial caries solution. Demineralized enamel depth was measured using by confocal laser scanning microscopy. The results were as follows. 1. The mean demineralized enamel depth was Group 1 (47.4㎛), Group 2 (61.8㎛), Group 3 (13.9㎛), Group 4 (8.2㎛). 2. The demineralized enamel depth was increased in Group 2 than Group 1 (p<0.05). 3. The demineralized enamel depth was reduced in Group 3 than Group1 and Group 2 with statistically significant differences (p<0.05). 4. Group 4 was very little demineralized. The results of the present study indicate that sealant application is useful for reducting enamel demineralization in orthodontic bracket bonding.

GPS 상시관측점을 이용한 차량항법 정확도 평가

이재원,송연경,김용석 東亞大學校 大學院 2004 大學院論文集 Vol.29 No.-

Nowadays it is necessary to manage the road system effectively because of the explosive increment of vehicle and goods. To resolve this problems through the fast upgrade of information about position and time of moving vehicle, the combined navigation system using GPS and complementary navigation system, i.e. INS, DR, etc. has been used. Although GPS is popular for the vehicle navigation system, this is not useful for the kinematic positioning of the vehicles in the urban canyon because of its few satellites. Therefore, this study deals with the kinematic positioning of the vehicles with GPS CORS to GPS navigation. For this, first the static single point positioning of GPS and GPS for reference station was performed to evaluate the accuracy of GPS positioning. Next, in the post-processed, the DGPS(Differential GPS) was performed for the kinematic positioning of the vehicles. So, it is expected that GPS CORS can be applicable to the control of traffic flow, the effective management of road system, and the development of ITS and it is regarded that the combined navigation system of vehicles with GPS, INS, and DR, etc. should be studied constantly.

복수과 진료 환자의 중복 처방에 대한 분석 및 평가

이지은,장혜경,오지영,유윤경,김현지,임숙인,연숙희,강진숙,최귀령 한국병원약사회 2003 병원약사회지 Vol.20 No.1

In an aging society, there is an increasing possibility of the duplication of the drugs given to patients because they take many kinds of drugs. Among the prescriptions given to the patients who was treated at multiple ambulatory clinics in St. Mary's hospital for one month of September, 2002, in which drugs that has identical or similar effects are prescribed, we analysed and evaluated them by patients' characteristics, kinds of medication, severity of side effects on a case by case basis. More, we assessed the potential additional costs. As a result of this study, the duplication rate turned out to be 6.69% and it was shown that the proportion of the elderly patients over 60 was high. The gastro-intestinal medications took up a large part and most of the cases showed that the danger caused by double taking of medicine was slight, but there were some examples in which the serious side effects were predicted. Expected additional costs were the average 11.349 won and it ranges from the minimum of 56 won to the maximum of 135,720 won. In conclusion, the management of drug histories of the patients who need the plural treatments is very important and the necessity of the individual and professional guidance of taking medicines for the elderly patients is emerged, too.

진공 Cavity상 멤브레인 구조를 가진 마이크로 유량센서의 단열특성

김석군,전치훈,김윤태,이종현 경북대학교 센서기술연구소 1998 센서技術學術大會論文集 Vol.9 No.1

We have proposed a surface-micromachined new microflow sensor to resolve the problems of poor thermal isolation and low sensitivity inherently in typical thermal flow sensors. A membrane of insulating films on a vacuum cavity supports a n'-poly heating resistor and two bidoped poly-Si thermophiles. We have investigated on heat-transfer characteristics of the sensor microstructure with the Sum-depth cavity of 100x100 μm^(2) by a two-dimensional numerical simulation as a function of the inside pressure of the cavity. As a result, the microstructure with the vacuum cavity shows better themal isolation effects than that of the air cavity.

강유전체 Pb(Zr_0.5₂ Ti_0.₄8)O₃박막의 성장 및 물리적 성질

표윤숙,김두회,이장기,김상수 昌原大學校 基礎科學硏究所 2000 基礎科學硏究所論文集 Vol.12 No.-

Ferroelectric Pb(Zr0.52Ti0.48)O3(PZT) thin films coated on Si(100), SiO2/Si(100), Pt/Ti/SiO2/Si(100) and ITO/glass substrates were perpared by sol-gel processing of metal alkoxide solutions and solvents. The XRD investigation shows that the tetragonal phase of polycrystalline PZT was formed by more than four times coating of the concentrated solution and by the heat treatment over 600℃. It was found by SEM that the thin film had an average thickness of about 900Å/coating and surfaces were crack-free and uniform. Capacitance and dielectric loss (tan δ) of PZT thin film were amounted to 0~30nF and 0.05∼20 in the region of 1 kHz ∼ 10 MHz respectively. From the hysteresis loops of PZT/Pt/Ti/SiO2/Si(100) and PZT/ITO/glass thin films, a remnant polarization of 0.2573 μC/cm2, 0.1873 μC/cm2 and a coercive field of 27.78 kV/cm, 24.44 kV/cm were obtained respectively. The band gap energy, refractive index, and extinction coefficient at λ =6235Å of the PZT thin films were 3.74 eV, 1.81 and 0.048 respectively.

PECVD로 제작한 수소화된 비정질 탄소(a-C:H) 박막의 화학결합 구조에 관한 연구

노지연,이석호,오동선,박혁렬,김영산 木浦大學校基礎科學硏究所 1997 基礎科學硏究誌 Vol.15 No.-

Hydrogenated amorphous carbon(a-C:H) films were prepared by PECVD(Plasma enhanced-chemical vapor deposition) method. The films were characterized using various spectroscopic measurements such as Raman scattering, FTIR(Fourier transform infrared) absorption spectroscopy. FRIR absorption reflects only the optically active hydrogen in the a-C:H and, using it together with Raman spectroscopy. The spectral analysis indicated that changes in Raman spectroscopy intensity occurring with increasing substrate temperature 50℃~300℃ were determined to be caused by changes in optically resonant components in the films. Furthermore, a significant correlation was the two crystalline forms of carbon-graphite and -diamond consist of sp² and sp³ bonds, respectively. Significant changes in chemical bonding(sp²/sp³) and micro-structure of hydrogenated amorphous carbon thin films occur as the substrate temperature varies from 50 to 300℃ during.

sol-gel법을 이용한 ?? 강유전체 박막의 성장과 특성

표윤숙,이장기,김상수 昌原大學校 基礎科學硏究所 1999 基礎科學硏究所論文集 Vol.11 No.-

We have developed a simple and rapid method for the synthesis of a precursor solution used in the production of ?? thin films of the layered-perovskite phase ??. Precursor solution preparation takes less than 30 min and involves the generation of two solutions : (a) Bi(O₂CMe)₃dissolved in pyridine and (b) ?? added to Sr(O₂CMe)₂and than solutbilized by HO₂CMe. After stirring separately for 10 min, these solutions are combined, stirred for an additional 10 min, and used without any further modifications. Films of the layered-perovskite phase were formed at temperatures as low as 700℃. Ferroelectric testing of SBN films, films, fired at 800℃ reveals standard hysteresis loop with no fatigue for up to 4×10 cycles.

연구개의 이소성 뇌조직 - 1예 보고-

최현주,이연수,김영신,이교영,강창석,심상인 대한병리학회 1998 Journal of Pathology and Translational Medicine Vol.32 No.11

오래 잠그어 둔 수도에서 유출되는 철분의 함량

송도미,이승희,신연욱,장혜경,오경숙,전현 효성여자대학교 가정대학 학도호국단 1984 家政大論集 Vol.3 No.-

This study given here presents the results of an experimental investigation of the iron contents contained in the city water which not used for a long time in the winter. The measurements were performed after turning on and for flowing water in a moment. And the quantitative analysis was studied by chemical form and the results were as follows. 1. The content of the ferrous ion was 113.8-14.4 ppm, water soluble iron was 22.5-14.4 ppm and acid soluble iron was 150-10 ppm. 2. Iron contents of the each chemical from gradually reduced but it appeared high value than standard value of city water until five minutes.