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Grease matrix as a versatile carrier of proteins for serial crystallography
Sugahara, Michihiro,Mizohata, Eiichi,Nango, Eriko,Suzuki, Mamoru,Tanaka, Tomoyuki,Masuda, Tetsuya,Tanaka, Rie,Shimamura, Tatsuro,Tanaka, Yoshiki,Suno, Chiyo,Ihara, Kentaro,Pan, Dongqing,Kakinouchi, Ke Nature Publishing Group 2015 NATURE METHODS Vol. No.
Serial femtosecond X-ray crystallography (SFX) has revolutionized atomic-resolution structural investigation by expanding applicability to micrometer-sized protein crystals, even at room temperature, and by enabling dynamics studies. However, reliable crystal-carrying media for SFX are lacking. Here we introduce a grease-matrix carrier for protein microcrystals and obtain the structures of lysozyme, glucose isomerase, thaumatin and fatty acid–binding protein type 3 under ambient conditions at a resolution of or finer than 2 Å.
Tanaka, Yasuo,Saito, Shohei,Mori, Shigeki,Aratani, Naoki,Shinokubo, Hiroshi,Shibata, Naoki,Higuchi, Yoshiki,Yoon, Zin Seok,Kim, Kil Suk,Noh, Su Bum,Park, Jong Kang,Kim, Dongho,Osuka, Atsuhiro WILEY-VCH Verlag 2008 Angewandte Chemie Vol.47 No.4
<B>Graphic Abstract</B> <P>Round the twist: Metalation of [36]octaphyrin 1 provided the Möbius aromatic Pd<SUB>2</SUB> complex 3 as well as the Hückel antiaromatic Pd<SUB>2</SUB> complex 2. This method can be applied to other expanded porphyrins and Group 10 metal ions. The aromatic/antiaromatic character was supported by NMR spectrscopy, NICS calculation, and two-photon absorption measurements. <img src='wiley_img/14337851-2008-47-4-ANIE200704407-content.gif' alt='wiley_img/14337851-2008-47-4-ANIE200704407-content'> </P>
Katsuyoshi Ando,Mikihiro Fujiya,Yoshiki Nomura,Yuhei Inaba,Yuuya Sugiyama,Takuya Iwama,Masami Ijiri,Keitaro Takahashi,Kazuyuki Tanaka,Aki Sakatani,Nobuhiro Ueno,Shin Kashima,Kentaro Moriichi,Yusuke Mi 대한장연구학회 2018 Intestinal Research Vol.16 No.3
Background/Aims: Venous thromboembolism (VTE) is a major extraintestinal manifestation in inflammatory bowel disease (IBD), regarded as an independent risk factor for VTE according to reports from Western countries. However, the incidence and risk factors of VTE in Asian IBD patients are not fully understood. We aimed to reveal the incidence and risk factors of VTE in Japanese IBD inpatients. Methods: The incidence of VTE in inpatients with IBD (n=340), gastrointestinal cancers (n=557), and other gastrointestinal diseases (n=569) treated at our hospital from 2009 to 2013 was retrospectively investigated. The characteristics and laboratory data of IBD inpatients with and without VTE were compared in univariate and multivariate analyses. Clinical courses of VTE in IBD were surveyed. Results: VTE was detected in 7.1% of IBD inpatients, significantly higher than in gastrointestinal cancer inpatients (2.5%) and inpatients with other gastrointestinal diseases (0.88%). The incidence of VTE in ulcerative colitis (UC) patients (16.7%) was much higher than that in those with Crohn’s disease (3.6%). In the univariate analysis, the risk factors were an older age, central venous catheter, prednisolone, surgery, low serum albumin, high serum C-reactive protein and D-dimer. According to a multivariate analysis, >50 years of age and surgery were the only risk factors. The in-hospital mortality rate of IBD inpatients with VTE was 4.2%. Conclusions: The incidence of VTE with IBD, especially UC, was found to be high compared with other digestive disease, which was almost equivalent to that of Western countries. The efficacy of prophylaxis needs to be investigated in Asian IBD patients.
Yoshihide Kanno,Tetsuya Ohira,Yoshihiro Harada,Yoshiki Koike,Taku Yamagata,Megumi Tanaka,Tomohiro Shimada,Kei Ito 대한소화기내시경학회 2018 Clinical Endoscopy Vol.51 No.3
Afferent loop syndrome is often difficult to resolve. Among patients with afferent loop syndrome whose data were extracted fromdatabases, 5 patients in whom metal stent placement was attempted were included and evaluated in this study. The procedure wastechnically successful without any adverse events in all patients. Metal stent(s) was placed with an endoscope in the through-the-scopemanner in 4 patients and via a percutaneous route in 1 patient. Obvious clinical efficacy was observed in all patients. Adverse eventsrelated to the procedure and stent occlusion during the follow-up period were not observed. Metal stent placement for malignantobstruction of the afferent loop was found to be safe and feasible.
Toshihiko Tomita,Hirokazu Fukui,Daisuke Morishita,Ayako Maeda,Yutaka Makizaki,Yoshiki Tanaka,Hiroshi Ohno,Tadayuki Oshima,Hiroto Miwa 대한소화기 기능성질환∙운동학회 2023 Journal of Neurogastroenterology and Motility (JNM Vol.29 No.1
Background/AimsDiarrhea-predominant irritable bowel syndrome (IBS-D)-like symptoms frequently occur in patients with quiescent Crohn’s disease (CD). To investigate the factors underlying IBS-D-like symptoms in patients with quiescent CD, we performed a comprehensive analysis of the clinical features and intestinal environment in those patients. MethodsWe performed a prospective observational study of 27 patients with quiescent CD (CD activity index [CDAI] ≤ 150; C-reactive protein ≤ 0.3 mg/dL). The presence and severity of IBS-D-like symptoms, health-related quality of life, disease-specific quality of life, and status of depression and anxiety were evaluated. The level of intestinal permeability, fecal calprotectin and organic acids and the profiles of gut microbiome were analyzed. ResultsTwelve of the 27 patients with quiescent CD (44.4%) had IBS-like symptoms, and these patients showed a significantly higher CDAI, IBS severity index and anxiety score than those without. The inflammatory bowel disease questionnaire score was significantly lower in the patients with IBS-D-like symptoms. There were no significant differences in small intestinal/colonic permeability or the levels of organic acids between the patients with and without IBS-D-like symptoms. Fusicatenibacter was significantly less abundant in the patients with IBS-D-like symptoms whereas their fecal calprotectin level was significantly higher (384.8 ± 310.6 mg/kg) than in patients without (161.0 ± 251.0 mg/kg). The receiver operating characteristic curve constructed to predict IBS-D-like symptoms in patients with quiescent CD using the fecal calprotectin level (cutoff, 125 mg/kg) showed a sensitivity and specificity of 73.3% and 91.7%, respectively. ConclusionMinimal inflammation is closely associated with the development of IBS-D-like symptoms in patients with quiescent CD.
Yoshihide Kanno,Tetsuya Ohira,Yoshihiro Harada,Shinsuke Koshita,Takahisa Ogawa,Hiroaki Kusunose,Yoshiki Koike,Taku Yamagata,Toshitaka Sakai,Kaori Masu,Keisuke Yonamine,Kazuaki Miyamoto,Megumi Tanaka,T 대한소화기내시경학회 2021 Clinical Endoscopy Vol.54 No.3
Background/Aims: The aim of this study was to evaluate the safety of sedation with propofol as an alternative to benzodiazepinedrugs in outpatient endoscopy. Methods: In this prospective study, examinees who underwent outpatient endoscopy under propofol sedation and submitted a nextdayquestionnaire with providing informed consent were evaluated. Periprocedural acute responses, late adverse events within 24hours, and examinee satisfaction were evaluated. Results: Among the 4,122 patients who received propofol in the 17,978 outpatient-based endoscopic examinations performedbetween November 2016 and March 2018, 2,305 eligible examinees (esophagogastroduodenoscopy for 1,340, endoscopicultrasonography for 945, and total colonoscopy for 20) were enrolled, and their responses to a questionnaire were analyzed. Themean propofol dose was 69.6±24.4 mg (range, 20–200 mg). Diazepam, midazolam, and/or pentazocine in combination withpropofol was administered to 146 examinees. Mild oxygen desaturation was observed in 59 examinees (2.6%); and mild bradycardia,in 2 (0.09%). Other severe reactions or late events did not occur. After eliminating 181 invalid responses, 97.7% (2,065/2,124) of thepatients desired propofol sedation in future examinations. Conclusions: Propofol sedation was found to be safe-without severe adverse events or accidents-for outpatient endoscopy on thebasis of the patients’ next-day self-evaluation. Given the high satisfaction level, propofol sedation might be an ideal tool for painlessendoscopic screening.
( Yukie Kidani ),( Yasuo Miki ),( Nana Nomimura ),( Shiori Minakawa ),( Norifumi Tanaka ),( Hiroshi Miyoshi ),( Koichi Wakabayashi ),( Yoshiki Kudo ) 대한산부인과학회 2016 대한산부인과학회 학술대회 Vol.102 No.-
Objectives: Hypoxic-ischemic encephalopathy (HIE) is a major cause of cerebral palsy in full-term infants. HIE occurs at a rate of about three per thousand live-born infants, even in developed countries. In recent years stem cell therapies have been applied in several fields of medicine. Endothelial progenitor cells (EPCs) are one of the major stem/progenitor cell subsets with the potential for repairing vascular injury. Therefore, we have speculated that the transplantation of CD133<sup>+</sup> cells, as a EPCs containing fraction, from umbilical cord blood could also be an useful therapy in perinatal hypoxia-induced brain injury. We established the ex vitro hypoxic-ischemic encephalopathy model to assess the effects of CD133<sup>+</sup> cells (endothelial progenitor cells) derived from human umbilical cord blood on nerve extension. In this study, we have investigated the therapeutic effects of CD133<sup>+</sup> cells for the treatment of neonatal HIE on an animal model. Methods: Hypoxic-ischemic brain lesions were induced in neonatal severe combined immunodeficiency mice using the Rice-Vanucci method. CD133<sup>+</sup> cells were administered by intraperitoneal injection 24 h after injury. Macroscopic assessment, growth evaluation and immunohistochemical analysis were performed. To evaluate motor function, rotarod test was performed every 7days between day 28 and day 56 of postnatal. Results: Immunohistochemical analysis revealed that intraperitoneally transplanted CD133<sup>+</sup> cells migrate towards the brain 48 h after injection. The ratio of lesioned to non-lesioned hemisphere area in the CD133<sup>+</sup> group was significantly higher than in the HI group. Moreover, in CD133<sup>+</sup> cell-treated animals, motor function improved and the brain was protected from the hypoxic-ischemic insult compared with untreated animals. Conclusion: Our results suggest that CD133<sup>+</sup> cells derived from human umbilical cord blood have therapeutic po-tential in neonatal hypoxic-ischemic encephalopathy.
무기 공정 모래 재생 처리에서의 습식 처리 조합과 배소 조건 검토
오구스 류야,다나카 요시키,토모치카 하야토,Ryuya Ogusu,Yoshiki Tanaka,Hayato Tomochika 한국주조공학회 2023 한국주조공학회지 Vol.43 No.5
이 보고서에서는 무기 공정에서의 무기 인공 재생 모래의 특성을 중심으로 다음과 같이 정리할 수 있다. 1) ICP 발광 분광 분석을 통한 재생 모래의 Na<sup>+</sup> 용출량, TP 휨강도 및 충전 밀동에는 높은 상관 관계가 확인되었다. 2) 현행 재생 방법 대비, 습식 연마 처리를 첨가함으로써 잔류 점결재가 제거되고 Na+ 용출량이 저감된 결과, 재생 모래의 TP 휨강도를 보다 개선 가능한 것이 확인되었다. 3) 재생 처리 공정 순서를 건식 연마 → 습식 연마 → 배소 처리로 함으로써, 가장 Na<sup>+</sup> 용출량이 낮고, 높은 휨강도를 얻을 수 있는 것이 확인되었다. 4) 습식 연마 단계를 거치지 않고 배소 처리 온도와 처리 시간을 궁리함으로써 충분한 품질의 재생 모래를 얻을 수 있는 가능성이 시사되었다. 마지막으로, 무기 공정은 유기 공정에 비해 압도적으로 냄새가 적고 생산성이 높으며 인공 모래와의 병용을 통한 재생 처리시의 폐기물량 저감, 주조품의 고품질화 등 이점이 기대되는 공정이다.
Current status of the diagnosis of chronic pancreatitis by ultrasonographic elastography
( Kazunori Nakaoka ),( Senju Hashimoto ),( Ryoji Miyahara ),( Hiroki Kawashima ),( Eizaburo Ohno ),( Takuya Ishikawa ),( Takamichi Kuwahara ),( Hiroyuki Tanaka ),( Yoshiki Hirooka ) 대한내과학회 2022 The Korean Journal of Internal Medicine Vol.37 No.1
Chronic pancreatitis (CP) is pathologically characterized by the loss of exocrine pancreatic parenchyma, irregular fibrosis, cellular infiltration, and ductal abnormalities. Diagnosing CP objectively is difficult because standard diagnostic criteria are insufficient. The change of parenchymal hardness is the key factor for the diagnosis and understanding of the severity of CP. The ultrasonography (US) or endoscopic ultrasonography (EUS) elastography have been used to diagnose pancreatic diseases. Both strain elastography (SE) and shear wave elastography are specific diagnostic techniques for measuring tissue hardness. Most previous studies were conducted with SE. There are three methods of interpreting SE; the method of recognizing the patterns in SE distribution images in the region of interest, the method of using strain ratio to compare the hardness of adipose tissue or connective tissue with that of the lesion, and the method of evaluating the hardness distribution of a target by histogram analysis. These former two methods have been used primarily for neoplastic diseases, and histograms analysis has been used to assess hardness distribution in the evaluation of CP. Since the hardness of the pancreas increases with aging, it is necessary to consider the age in the diagnosis of pancreatic disorders using US or EUS elastography.