갑상선 여포선암 진단시 HBME-1 면역염색법의 유용성

신영구,이기범,정윤석,김현만 대한내분비학회 2000 Endocrinology and metabolism Vol.15 No.4·5

Aspegillus nidulans 에서 정단성장이상 돌연변이주의 세포벽 구조 분석

정윤신,한갑훈,한동민 원광대학교 생명공학연구소 1997 생명공학연구소보 Vol.5 No.1

To investigate the effects of the cell wall on the pigmentation and branching in Aspergillus nidulans, the chemical composition and ultrastructure of cell wall in wild type (FGSC4), null pigmentation mutant (NPG, WX17) and suppressor of the null pigmentation mutation (SU-NPG, SU602) have been examined. The rate of protoplast formation of NPG and SU-NPG was faster than that of wild type, suggesting that cell wall structure of NPG and SU-NPG should be modified. Through the chemical analysis of cell wall it was found that the colorless phenotype of null pigmentation mutant was due to the lack of melanin in the hyphal wall. The amounts of alkali-soluble fraction, in which major component is thought to be glucan, in the cell wall extract of NPG and SU-NPG were less than that of wild type. The lack of melanin and glucan in the mutant hyphal wall might be the reason why the cell wall of mutants was more readily hydrolyzed than that of wild type when treated with the cell wall lytic enzyme Novozyme 234. Scanning electron micrographs (SEM) revealed that the most outer layer of conidia wall peeled off in NPG and SU-NPG on the 6th day from the complete of conidiation. And also SEM showed that hyphal growth and branching were developed not very well in NPG and SU-NPG. Transmission electron micrographs (TEM) showed that the plasma membrane was crenulated in wild type conidia, but that of NPG and SU-NPG was not. TEM also represented that the hyphal wall in SU-NPG was thicker tha/#n that of wild type and NPG. Also, tips were round-shaped in SU-NPG, but not in NPG and wild type. These results from electron microscope indicated that the ultrastructure of cell wall of mutants was modified.

일부 지역 농촌 노인의 철분 영양상태에 관한 연구

신소영,최미경,이윤신,승정자 동아시아식생활학회 1996 동아시아식생활학회지 Vol.6 No.2

The purpose of this study was to examine nutritional status of iron in 27 rural elderly subjects. The daily intake, urinary excretion and serum level of iron were determined by 24-hr food collection method, and 24-hr urine and serum collection for 3 days. The results were summarized as follows. 1. The mean age, height, weight, and blood pressure were 67.7 years, 163.2㎝, 55.9㎏, and 121.1/80.0㎜Hg in men and 69.9 years, 146.5㎝, 47.6㎏, and 108.9/71.1㎜Hg in women, respectively. 2. The daily intake of energy, protein, vitamin A, vitamin B₁, vitamin B₂, niacin, vitamin C, and iron were lower than Korean recommended dietary allowances. 3. The daily iron intake was 7.82㎎. The proportion of heme iron and nonheme iron to the total iron intake were 8.05% and 91.94%, respectively. Major iron sources of the rural elderly were cooked rice, Ko Chu Jang, beef blood, anchovy, soybean paste, and leafy radish. 4. The hemoglobin, hematocrit, serum iron, and total iron binding capacity were 14.3g/dl, 42.9%, 79.4 ㎍/dl, and 268.4 ㎍/dl in men and 12.9g/dl, 38.8%, 92.4 ㎍/dl, and 277.9 ㎍/dl in women, respectively. 5. There was a significant positive correlation between dietary intake and urinary excretion of iron.

시루봉地域一帶의 森林植生에 關한 硏究 : 天然林 및 人工林의 林分構造를 中心으로 Focused on Forest Stand Structure of Natural Artifical Forests

정윤수,신수철 동국대학교 생명자원과학대학 연습림 1997 연습림논문집 Vol.- No.5

To investigate the structure of the forest vegetation of Mt. Sirubong area in Chungbuk district, fifteen plots of natural forest and five plots of artificial forest were set up. As the result of classification and ordination analysis the study area was divided into six groups, which were Quercus mongolica community, Q. serrata community, Pinus densiflora community, Larix leptolepis community, P, rigida community and P. koraiensis community. Species diversity indices range of each of the communities was 0.8686∼1.2403, Quercus mongolica community showed the highest value and P. rigida community showed the lowest value among the communities. The similarity indices range were 17.14∼81.29%, between Quercus serrata community and Pinus densiflora community showed the highest value while on the other between Quercus serrata community and Larix leptolepis community showed the lowest value among the communities. The comparision of site index between Mt. Sirubong area with nationwide, Pinus koraiensis was the highest site index and P. rigida was above the average and Larix leptolepis was the average. Pinus densiflora showed below the average compared with nationwide was caused by the decline species of successional trends in the middle temperated forest zone. According to analysis of annual ring growth of major tree species by tree ring chronology, growth­decrease was occured in common in the year of 1974∼75, 1983∼85, 1987∼88, 1991 and 1995, this was highly concerned with the amount of precipitation during the period of from march to November.

생체분자 분리 및 분석에 있어서의 마이크로-나노 시스템

정우재,신동식,이윤식 한국공업화학회 2002 공업화학 Vol.13 No.8

휴먼게놈 프로젝트가 종료됨에 따라, 우리 몸의 대사ㆍ생리 작용, 질병에 직접 관여하고 있는 단백질의 분리와 구조 분석에 관심이 집중되고 있다. 현재 프로테오믹스의 근간이 되고 있는 2D-젤 전기영동법은 긴 소요시간, 복잡한 과정, 재현성 문제, 검출한계 등 그 문제점이 지적되고 있으며, 이를 해결하기 위한 마이크로-나노 대체 기술들이 최근 속속 보고되고 있다. 이에 본 총설에서는 단백질을 포함한 생체분자의 분리ㆍ검출 및 미량 분석을 위한 마이크로-나노 시스템 기술들의 최신 연구동향을 알아보고자 한다. 구체적으로는 마이크로 비드 어세이를 기반으로 하는 마이크로 분석 시스템, 나노 테크놀로지를 이용한 바이오 센서 또는 바이오 칩 분야와 마이크로 어레이 기술 등과 그 응용을 중심으로 최근 2∼3년간 중요 논문에 보고된 내용을 소개함으로써 최근 발전하고 있는 마이크로-나노 기술의 중요성에 대해서 언급하고자 한다. In the post-genomic era, purification and structure identification of proteins that are related to cell functions and diseases became the most important research topics. Although 2D-gel electrophoresis is currently the most widely accepted technique for the separation of complex protein mixtures, it still possesses some drawbacks to overcome. Being very labor intensive, it is time consuming, difficult to obtain reproducible results, and has low detection limit. Recently, various methods that utilize micro/nano systems to separate and analyze biomolecules including proteins appeared to overcome these drawbacks. In this report, we overviewed recent research trends of micro/nano bio field, by reviewing recent papers for the last 2∼3 years, and emphasized the importance of the development of bead based micro analysis system, nanotechnology for biosensing, and protein micro array system.

영구치 치수 기질세포를 이용한 연골 분화 및 분화 시기에 따른 형태학적 변화

정주령,김하나,박열,김민정,오영주,신수정,최윤정,김경호 大韓齒科保存學會 2012 Restorative Dentistry & Endodontics Vol.37 No.1

Objectives: The aim was to confirm the stem cell-like properties of the dental pulp stromal cells and to evaluate the morphologic changes during in vitro chondrogenesis. Materials and Methods: Stromal cells were outgrown from the dental pulp tissue of the premolars. Surface markers were investigated and cell proliferation rate was compared to other mesenchymal stem cells. Multipotency of the pulp cells was confirmed by inducing osteogenesis, adipogenesis and chondrogenesis. The morphologic changes in the chondrogenic pellet during the 21 day of induction were evaluated under light microscope and transmission electron microscope. TUNEL assay was used to evaluate apoptosis within the chondrogenic pellets. Results: Pulp cells were CD90, 105 positive and CD31, 34 negative. They showed similar proliferation rate to other stem cells. Pulp cells differentiated to osteogenic, adipogenic and chondrogenic tissues. During chondrogenesis, 3-dimensional pellet was created with multi-layers, hypertrophic chondrocyte-like cells and cartilage-like extracellular matrix. However, cell morphology became irregular and apoptotic cells were increased after 7 day of chondrogenic induction. Conclusions: Pulp cells indicated mesenchymal stem cell-like characteristics. During the in vitro chondrogenesis, cellular activity was superior during the earlier phase (within 7 day) of differentiation.

과학 성취도에 영향을 미치는 전두엽 연합령의 기능에 대한 종단적 연구

김영신,권용주,박윤복,구수정,정완호 한국교원대학교 과학교육연구소 2000 청람과학교육연구논총 Vol.10 No.1

The purpose of this study was to longitudinally analyze influences of functional prefrontal lobe on science achievement in middle school students. In this longitudinal study, 47 students(male 19, female 27) were sampled in local area. The students were administered test of GALT(group assessment of logical thinking), CTST(classroom test of scientific test), FIT(figural intersection test), GEFT(group embedded figural intersection test), TOL(tower of London), WCST(Wisconsin card sorting test) at December 1997 and 1999. It is substitute science achievement for science grade each year. The resulted that prefrontal lobe was influenced upon science achievement. Science achievement statistically significant correlated with functional prefrontal lobe, specially logical thinking level, scientific reasoning, perseveration error. And, the development of functional prefrontal lobe functions did not only linear, but also each function has differ with development speed and period. School science classroom was suited high level students. Who has been grade C and D in first-grade, they did not improved. A first-grade achievement effect on science achievement. And also scientific reasoning ability effect on science achievement.

인슐린의존형 당뇨병환자에서 임신중 병발한 당뇨병성 케톤산증 1예

이성근,신영구,이성규,정윤석,이관우,김현만,김행수 아주대학교 1997 아주의학 Vol.2 No.2

저자등은 인슐린의존형 당뇨병환자에서 임신중에 병발한 당뇨병성 케톤산증을 1예를 경험하였기에 문헌고찰과 함께 보고하는 바이다. IDDM is an autoimmune disease resulted from beta cell destruction and insulin deficiency, and developed mostly in children and puberty. Ketoaddosis remains one of the most senous complications that can occur in the pregnant diabetic. A 26-year-old woman, gravida 1, para 0, was admitted at 32 weeks gestation because of fever lasted for 6 days. She had been treated with insulin pump during the pregnancy. Blood sugar was well controlled before admission, and the etiology of her ketoacidosis was urinary tract infection and no insulin-intake. DKA was treated with fluids and intravenous insulin. Urinary tract infection was treated with antibiotics, based on culture test. Healthy newbom was later delivered by cesarean section due to fetal distress. We experienced a case of DKA in pregnant woman and report it with a review on literature.

관절외 근골격계의 염증성 질환에 대한 Tenoxicam (Ro 12-0068)의 임상적 시험결과 : Tenoxicam 20mg과 Piroxicam 20mg의 평행 이중 맹검법에 의한 시험관찰

노성만,정재윤,김신곤 최신의학사 1987 最新醫學 Vol.30 No.4

於靑島 海松林의 植生構造에 關한 硏究

金昌浩,辛壽哲,金熙采,鄭潤軾 동국대학교 생명자원과학대학 연습림 1995 연습림논문집 Vol.- No.4

This study was carried out to understand the vegetation structure of Pinus thunbergii forest on O˘cho˘ng island and the results were summarized as following: 1. The most dominant species on O˘chong island was Pinus thunbergii and the Ⅰ.Ⅴ. of Pinus thunbergii in the northern aspect showed higher than that of southeren aspect. Quercus acutissima was emerged to the Pinus thunbergii forest and the Ⅰ.Ⅴ. of Quercus acutissima was higher than that of Pinus thunbergii in shrub layer. In addition, the Ⅰ.Ⅴ. of Eurya japonica was the highest among shrubs. The Ⅰ.Ⅴ. of Castanopsis cuspidata var. sieboldii was the highest among evergreen broad leaved trees. 2. The sites investigated were grouped by Cluster Analysis into Pinus thunbergii forest and mixed-species forest in which Castanopsis cuspidata var. sieboldii, Carpinus coreana, and Quercus acuta are dominant. The factor affecting the most remarkable difference might be the silt between Pinus thunbergii forest and the mixed-species forest. 3. As results of COA Odination, community ordination was appeared to Pinus thunbergii area and mixed-species area. In case of the species ordination all the woody species were closely coordinated except Quercus acuta, Meliosma oldhamii, Quercus variabilis. Sand and organic matter were appeared as the most influencing factors to Ⅱ and Ⅲ axes, respectively. 4. The Ⅰ.Ⅴ. of Pinus thunbergii might be decreased by scarce reproduction in the shrub layer. On the other hand, Quercus acutissima, Castanopsis cuspidata var. sieboldii, and Carpinus coreana would increase and succession would be completed in some area by those species. 5. The content of NaCl in soil was much higher than that of land. Soil pH showed significant difference at 5% between the northern and the southern aspect and also sand, silt, Avail. P_2O_5. K^+, and Na^+ were showed significance 5% between Pinus thunbergii forest and mixed-species forest in which Castanopsis cuspidata var. sieboldii, Carpinus coreana, Quercus acuta are dominant. 6. The mainly emergent woody species in O˘chong island - Pinus thunbergii, Quercus acutissima, Q.acuta, Castanopsis cuspidata var. sieboldii, Carpinus coreana. Eurya japonica, Callicarpa japonica, Vibumum dilatatum, and so on - are strong in salinity. Threrfore, the vegetation of O˘chong island was severely influenced by sea breeze and NaCl in soil.