RISS 학술연구정보서비스

검색
다국어 입력

http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.

변환된 중국어를 복사하여 사용하시면 됩니다.

예시)
  • 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
  • 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
닫기
    인기검색어 순위 펼치기

    RISS 인기검색어

      검색결과 좁혀 보기

      • 좁혀본 항목

      • 좁혀본 항목 보기순서

        • 원문유무
        • 음성지원유무
          • 원문제공처
          • 등재정보
            • 학술지명
            • 주제분류
            • 발행연도
            • 작성언어
            • 저자

          오늘 본 자료

          • 오늘 본 자료가 없습니다.
          더보기
          • 무료
          • 기관 내 무료
          • 유료
          • Cloning and Sequencing of Breed specific DNA Fragment in Horse

            Cho,,Byoung,Wook 밀양대학교 농업기술개발연구소 2001 農業技術開發硏究所報 Vol.5 No.1

            본 연구는 말의 품종을 구분하는 품종 특이 표지인자를 클로닝하고, 염기서열을 분석하기 위하여 수행되었다. 임의의 Primer 800개중 8개(MG 162, MG 030, MG 549, MG 126, MG 065, MG 562, MG 244, MG 053)가 품종 특이 표지인자로 관찰되었으며 이들 중 MG 053, M 030은 품종 특이 표지인자로 C'클로닝 및 염기서열을 분석한 결과 품종 특이 표지인자로 판명되었다. RAPD(Random amplified polymorphic DNA) markers generated by shorts oligonucleotide primers of random sequences have been successfully employed to detect genetic polymorphims between horse breeds. We were able repidly to identify markers which distinguish Cheju horse from Thoroughbred, or other crossbred PCR products from eigh primers (MG 162, MG 030, MG 549, MG 126, MG 065, MG 562, MG 244, MG 053). Clearly identified marker differentiation between horse breeds. The primer MG 053 of ten generated polymorphic DNA fragment were specific for Thoroughbred, being present only in Thoroughbred but absent in Cheju horse and the primer MG 030 of ten generated polymorphic DNA fragment were specific for Cheju horse, being present only in Cheju horse but absent in Thoroughbred horse Among 8 random primers, 3 primers were Thoroughbred specific and f primers were Cheju-native horse specific. Testing individual horse revealed that 6 marker showed the similar band pattern between Cheju-native horse and Thoroughbred. However, 2 marker were wholly absent in breed while present in the other breed. MG 053 detracted only Thoroughbred and MG 030 was detected Cheju-native horse, respectively. We cloned horse specific allelic marker (MG 053 and MG 030). These results demonstrates that non repetitial sequence. Therefore, these RAPD bands specifie to breed have potential possibility for genetic marker for specific breed. RAPD bands specifie to breed have potential possibility for genetic marker for specific breed. RAPD bands specific for Cheju horse could be used to distinguish the Cheju horse from Thoroughbred or other crossbred.

          • 光 强度別 아주까리의 光合成速度와 氣孔傳導度의 變化 및 相互聯關性

            韓英熙,崔仁洙,朴賢哲,金成萬,金容澈,李忠烈 밀양대학교 농업기술개발연구소 2000 農業技術開發硏究所報 Vol.4 No.1

            The Ricinus communis L. was planted 70cm x 70cm in field to investigate on the changes of photosynthesis followed by the investigation on the light intensity. The photosynthetic rate and stomatal conductance were increased as the PAR was increased to 2000μ mol/m2/s in leaves of all. A linear equation were obtained between net photosynthetic and transpiration rate. The quadratic equations were obtained between net photosynthetic rate and stomatal conductance, and a linear equation was obtained between transpiration rate and stomatal conductance. It was also confimed that intercellular CO2 concentration was significantly correlated with net-photosynthetic rate and stomatal conductance under low light intensity.

          • 작약의 RAPD 분석을 위한 PCR 최적조건 구명

            최인수,김성만,김용철,이충렬,박현철 밀양대학교 농업기술개발연구소 2000 農業技術開發硏究所報 Vol.4 No.1

            To optimize the PCR condition is one of the most important steps for RAPD analysis. The purpose of this study was to optimize PCR condition in Paeonia. 3×3×3 factorial experiment for template DNA concentration, MgCl2 concentration, and amount of taq polymerase was conducted. Another factorial experiment for reaction temperature(denature, annealing, and extension) was also conducted. The most appropriate template DNA concentration was 60ng. Clear bands were observed from 2.5mM and 4.5mM of MgCl2 if template DNA concentration and amount of taq polymerase were proper. Amount of taq polymerase for the optimal PCR condition was 0.5unit and 1unit. In the consideration of results from template DNA concentration, MgCl2 concentration, and amount of taq polymerase, 4 conditions (60ng of template DNA, 2.5mM MgCl2 and 0.5unit taq polymerase; 60ng of template DNA, 4.5mM MgCl2 and 0.5unit taq polymerase; 60ng of template DNA, 4.5mM MgCl2 and lunit taq polymerase; and 40ng of template DNA, 4.5mM MgCl2 and 1unit taq polymerase) were best combinations for the optimal PCR condition. Reaction temperature for the optimal PCR condition was 92℃, 36℃, 72℃.

          • 작약지배지에서의 식물기생선충 발생 및 피해

            박현철,김성만,김용철,이충열,최인수 밀양대학교 농업기술개발연구소 2000 農業技術開發硏究所報 Vol.4 No.1

            A study on the damages and occurrence of phytophagous nematodes was carried out in the medicinal herb (Paeonia lactiflora) fields at Andong, Kyungbuk. According to the investigation of the nematode population, Meloidogyne spp. was dominated in the sampling area, followed by Helicotylenchus spp., Pratylenchus spp. and Tylenchus spp. among the phytophgous nematodes. However, Rhabditis spp., which is not causing any damages for the crops, were highly dominated among the total nematode population in the sampling area. Soil characteristics affected nematode distribution and population. The number of the nematode was 300 per 300g of soil within 5cm from the surface at the sampling area, and the nematodes were distributed even under 50cm.

          • 복숭아의 PCR 최적 조건 구명

            공현정,박진철,김성만,김용철,이충열,박현철,최인수 밀양대학교 농업기술개발연구소 2000 農業技術開發硏究所報 Vol.4 No.1

            To optimize PCR condition is the most important and fundamental step for experiments using PCR. The purpose of this study was to optimize PCR condition for RAPD analysis in peach. 3×3×3 factorial experiment for template DNA concentration, MgCl2 concentration, and amount of taq polymerase was conducted. Another factorial experiment for denature, annealing, extension temperature on the optimal PCR condition was also conducted. The best template DNA concentration was 20ng(0.5unit taq polymerase and 2.5mM MgCl2), 40ng(0.5unit taq polymerase and 4.5mM MgCl2, and 1unit taq polymerase and 2.5mM MgCl2), and 60ng(0.5unit taq polymerase and 2.5mM MgCl2, 0.5unit taq polymerase and 4.5mM MgCl2, 1unit taq polymerase and 2.5mM MgCl2, 1unit taq polymerase and 4.5mM MgCl2, and 1unit taq polymerase and 7.0mM MgCl2). The best MgCl2 concentration was 2.5mM(40ng template DNA and 1unit taq polymerase, 60ng template DNA and 0.5unit taq polymerase, and 60ng template DNA and 1unit taq Folymerase), and 4.5mM(40ng template DNA and 0.5unit taq polymerase, 40ng template DNA and 1unit taq polymerase, 60ng template DNA and 0.5unit taq polymerase, and 60ng template DNA and 1unit taq polymerase), and 7.0mM (60ng template DNA and 1unit taq polymerase). The best taq polymerase concentration was 0.5unit(20ng template DNA and 2.5mM MgCl2, 40ng template DNA and 4.5mM MgCl2, 60ng template DNA and 2.5mM MgCl2, and 60ng template DNA and 4.5mM MgCl2), and 1unit(40ng template DNA and 2.5mM MgCl2, 60ng template DNA and 2.5mM MgCl2, 60ng template DNA and 4.5mM MgCl2, and 60ng template DNA and 7.0mM MgCl2). When we consider results from template DNA concentration, MgCl2 concentration, and amount of taq polymerase, 5 conditions(60ng of template DNA, 7.0mM MgCl2 and 1unit taq polymerase; 60ng of template DNA, 4.5mM MgCl2 and 1unit taq polymerase; 60ng of template DNA, 2.5mM MgCl2 and 0.5unit taq polymerase; 40ng of template DNA, 2.5mM MgCl2 and 1unit taq polymerase; and 40ng of template DNA, 4.5mM MgCl2 and 0.5unit taq polymerase;) were best combinations for the optimal PCR condition. Reaction temperatures for the optimal PCR condition were 90℃, 40℃, 72℃ and 92℃, 36℃, 72℃.

          • 참외의 PCR 최적 조건 구명

            공현정,김용철,최인수 밀양대학교 농업기술개발연구소 2000 農業技術開發硏究所報 Vol.4 No.1

            This study was conducted to identify the optimized PCR condition in cucumis melo. Factorial experiments for template DNA concentration, MgCl2 concentration, amount of taq polymerase, and reaction temperature(denature, annealing, extension) were conducted. The best template DNA concentration was 40ng(0.5unit taq polymerase and 4.5mM MgCl2, and 1unit taq polymerase and 2.5mM MgCl2), 60ng(0.5unit taq polymerase and 2.5mM MgCl2, 0.5unit taq polymerase and 4.5mM MgCl2, 1unit taq polymerase and 2.5mM MgCl2, 1unit taq polymerase and 4.5mM MgCl2, and 1unit taq polymerase and 7.0mM MgCl2). The best MgCl2 concentration was 2.5mM(40ng template DNA and 1unit taq polymerase, 60ng template DNA and 0.5unit taq polymerase, and 60ng template DNA and 1unit taq polymerase)와 4.5mM(40ng template DNA and 0.5unit taq polymerase, 60ng template DNA and 0.5unit taq polymerase, and 60ng template DNA and 1unit taq polymerase), and 7.0mM(60ng template DNA and 1unit taq polymerase). The best amount of taq polymerase was 0.5unit(40ng template DNA and 4.5mM MgCl2, 60ng template DNA and 2.5mM MgCl2, and 60ng template DNA and 4.5mM MgCl2) and 1unit(40ng template DNA and 2.5mM MgCl2, 40ng template DNA and 4.5mM MgCl2, 40ng template DNA and 7.0mM MgCl2, 60ng template DNA and 2.5mM MgCl2, 60ng template DNA and 4.5mM MgCl2, and 60ng template DNA and 7.0mM MgCl2). When we consider results from template DNA concentration, MgCl2 concentration, and amount of taq polymerase, 6 conditions(60ng template DNA, 7.0mM MgCl2, 1unit taq polymerase; 60ng template DNA, 4.5mM MgCl2, 1unit taq polymerase; 60ng template DNA, 2.5mM MgCl2, 1unit taq polymerase; 60ng template DNA, 4.5mM MgCl2, 0.5unit taq polymerase; 60ng template DNA, 2.5mM MgCl2, 0.5unit taq polymerase; and 40ng template DNA, 2.5mM MgCl2, 1unit taq polymerase) were best combinations for the optimal PCR condition. Reaction temperatures for the optimal PCR condition were 90℃, 40℃, 72℃ and 92℃, 36℃, 72℃.

          • 무궁화의 PCR 최적조건 구명

            최재희,최인수 밀양대학교 농업기술개발연구소 2000 農業技術開發硏究所報 Vol.4 No.1

            To optimize the PCR condition is the most fundamental and important procedure for the further analysis such as RAPD. This study was conducted to optimize PCR condition in Hibiscus syriacus. 3×3×3 factorial experiment for template DNA concentration, MgCl2 concentration and amount of taq polymerase was conducted. Another factorial experiment for reaction temperature(denature, annealing, extension) was also conducted. The most clear and reproducible bands were identified from 60ng template DNA, those combinations were 60ng(0.5unit taq polymerase and 2.5mM MgCl2, 0.5unit taq polymerase and 4.5mM MgCl2, 1unit taq polymerase and 2.5mM MgCl2, and 1unit taq polymerase and 7.0mM MgCl2). Clear bands were observed from 2.5mM and 4.5mM of MgCl2 concentration, those combinations were 2.5mM(60ng template DNA and 0.5unit taq polymerase, 60ng template DNA and 1 unit taq polymerase), and 4.5mM(60ng template DNA and 0.5unit taq polymerase). Amount of taq polymerase for the optimal PCR condition was 0.5unit and 1unit, the best combinations were 0.5unit(60ng template DNA and 2.5mM MgCl2, 60ng template DNA and 4.5mM MgCl2), and 1unit(40ng template DNA and 7.0mM MgCl2. 60ng template DNA and 2.5mM MgCl2 and 60ng template DNA and 7.0mM MgCl2). When we consider results from template DNA concentration, MgCl2 concentration, and amount of taq polymerase, 3 conditions(60ng of template DNA, 2.5mM MgCl2 and 0.5unit taq polymerase; 60ng of template DNA, 4.5mM MgCl2 and 0.5unit taq polymerase; and 60ng of template DNA, 2.5mM MgCl2 and 1unit taq polymerase) were best combinations for the optimal PCR condition. Reaction temperatures for the optimal PCR condition were 86℃, 35℃, 64℃ ; 90℃ , 40℃, 72℃ ; and 92℃, 36℃, 72℃

          • 오이의 RAPD분석을 위한 PCR 최적화

            공현정,최인수 밀양대학교 농업기술개발연구소 2000 農業技術開發硏究所報 Vol.4 No.1

            This study was conducted to identify the best combinations of factors(template DNA concentration, MgCl2 concentration, and amount of taq polymerase) for the optimization of PCR in cucumber. 3×3×3 factorial experiment for template DNA concentration, MgCl2 concentration, and amount of taq polymerase was conducted. Another factorial experiment for denature, annealing, extension temperature on the optimal PCR condition was also conducted. The most clear and reproducible bands were appeared in 60ng(0.5unit taq polymerase and 2.5mM MgCl2, 0.5unit taq polymerase and 4.5mM MgCl2, and 1unit taq polymerase and 7.0mM MgCl2). The best MgCl2 concentration was 2.5mM(60ng template DNA and 0.5unit taq polymerase), 4.5mM(40ng template DNA and 0.5unit taq polymerase, 40ng template DNA and 1unit taq polymerase, and 60ng template DNA and 0.5unit taq polymerase), and 7.0mM(60ng template DNA and 1unit taq polymerase). The combinations which showed the best bands from the amount of taq polymerase were 0.5unit(60ng template DNA and 2.5mM MgCl2, 60ng template DNA and 4.5mM MgCl2) and 1unit(40ng template DNA and 4.5mM MgCl2, 60ng template DNA and 2.5mM MgCl2, and 60ng template DNA and 7.0mM MgCl2). When we consider results from template DNA concentration, MgCl2 concentration, and amount of taq polymerase, 3 conditions(60ng of template DNA, 2.5mM MgCl2 and 0.5unit taq polymerase; 60ng of template DNA, 4.5mM MgCl2 and 0.5unit taq polymerase; 60ng of template DNA, 7.0mM MgCl2 and 1unit taq polymerase) were best combinations for the optimal PCR condition. Reaction temperatures for the optimal PCR condition were 86℃, 35℃, 64℃; 90℃, 40℃, 72℃; and 92℃, 36℃, 72℃.

          • 한우에 있어서 GnRH와 PGF_2α에 의한 발정동기화 연구

            강한석,김선구 밀양대학교 농업기술개발연구소 2000 農業技術開發硏究所報 Vol.4 No.1

            This study was conducted to get some information for the timed insemination program development of Korean native cows. Korean native cows that didn't show estrus behavior up to 60days after calving were used. Estrus synchtonization was induced by injection of PGF2α and GnRH. The estrus synchronization rate was 40.0% in treatment of PGF2α- PGF2α and 93.3% in treatment of GnRH- PGF2α -GnRH. All synchronization cows ovulated between 26 and 32hrs after the 2nd injection of GnRH and the highest ovulation rate was shown at 28hrs after the 2nd injection of GnRH. The highest pregnancy rate was shown in case of artificial insemination at 24hrs after the 2nd injection of GnRH. The pregnancy rate was 50.7% in treatment of PGF2α - PGF2α and 76.9% in treatment of GnRH- PGF2α -GnRH.

          • 광주기에 따른 누에번데기 동충하초의 생장양태

            이은하,이상몽 밀양대학교 농업기술개발연구소 2000 農業技術開發硏究所報 Vol.4 No.1

            Host-pathogen relationship and comparison of inoculation methods of the six entomopathogenic fungi strains (three of Paecilomyces japonica, J2, C24O, C66O; two of Cordyceps militsris, C18, C738; one of Cordyceps scarabaeiclola, C252) to the silkworm, Bombyx mori were investigated. 1.All the six entomopathogenic fungi tested in the present study infected their host, silkworm, Bombyx mori, through the larval or the pupal stages. 2.Time of inoculation (or germination) on the host silkworm was at the 5th larval or pupal stages for all the fungi. 3.The Paecilomyces strains, J2, C24O, C66O showed higher infection ratio than the Cordyceps militaris C18, C738 and Cordyceps scarabaeicola C252. 4.The degree of infection level on the silkworm varied according to inoculation methods used. 5.Time required for fruiting-body formation was longer in the method "Spray" than in the method "injection or immersion"

          맨 위로 스크롤 이동