한국인에서 Quadruplex PCR을 이용한 8종류 상염색체 STR 유전좌의 다형성 분석

장영길,김기범,신창호,이숭덕,이윤성,이정빈 大韓法醫學會 2001 대한법의학회지 Vol.25 No.1

Two quadruplex PCR reactions were designed for 7 tetrameric (D3S2406, D4S2368, D5S818, D7S821, D9S925, D13S317, D19S253) and one trimeric (D6S1043) short tandem repeats loci to study the allele frequency and the applicability of genetic variation in these loci in forensic case works. For 310 unrelated Koreans DNA was isolated from peripheral blood using phenol/chloroform method. Quadruplex I was consisted of D4S2368, D6S1043, D7S821, D9S925 and quadruplex II D3S2406, D5S818, D13S317, D19S253. The amplified products were analyzed by 5% polyacrylamide gel electrophoresis followed by silver staining. The heterozygosity in each loci ranged 92.91-66.13%, and PD(Power of Discrimination) was above 085 in each loci. Every loci except D6S1043 followed hardy-Weinberg expectation. The cumulative PI was low as 1.65×10-10. Two mutations were noted, one in D19S253 and the other in D9S925 among 234 gametes. With these results above eight STR loci studied here preyed to be highly polymorphic enough to be used in forensic field. This study provides valuable population data in these loci for Korean.

비납솔더를 이용한 솔더접합부의 신뢰성에 관한 연구

하범용,이준환,윤준호,신영의 중앙대학교 기술과학연구소 2000 기술과학연구소 논문집 Vol.30 No.-

본 연구에선 실제적으로 전자 제품에 사용되고 있는 패키지(QFP : 피치간격 0.8mm, TSOP : 피치간격 0.4mm)를 사용하여 Sn/Ag 공정 솔더 페이스트를 사용하여 온도 조건에 따른 솔더 접합부의 초기 접합 강도를 45°Peel test를 통해 측정하였으며, Aging시간을 변수로 하여 솔더와 구리 합금 사이에서 생성되는 IMC층의 두께를 측정, 분석하여 Sn/Pb 공정 솔더 페이스트를 사용했을 경우와 비교, 검토하였다. 실험결과로부터 비납솔더인 Sn/Ag 공정 솔더를 사용하여 접합부의 품질 및 장기신뢰성(고온유지) 측면에서 만족할만한 결과를 얻었으며, Sn/Pb 공정솔더의 대체 재료 가능성을 실험을 통하여 입증하였다. As solder joint become small and fine, the reliability and solderability of solder joint is the critical issue in present electronic packaging industry. Besides the use of lead(Pb) containing solders for the interconnections of microelectronic subsytem assembly and packaging has enviromental issue. In this study, using Sn/Pb and Sn/Ag eutectic solder paste, to obtain decrease of solder joint strength with aging time, initial solder joint strength and aging strength after 1000 hour aging at 100℃ were measured by peel test. And to obtain the growth of intermetallic compound(IMC) layer thickness, IMC layer thickness was measured by scanning electron microscope(SEM). As a result, solder joint strength was decrease with aging time increasing. The mean IMC layer thickness was increased linearly with the square root of aging time. The diffusion coefficient(D) of IMC layer was found to 1.29×10 exp (-13) ㎠/s at using Sn/Pb solder paste, 7.56×10 exp (14) ㎠/s at using Sn/Ag solder paste.

정신분열병 환자에서 통계적 확률 해부학 지도를 이용한 확산텐서영상 연구 : 겉보기 확산 계수 지도를 이용한 고찰

홍순범,신용욱,김대진,문원진,정은철,이재성,박해정,권준수 大韓神經精神醫學會 2006 신경정신의학 Vol.45 No.4

Objectives : Diffusion tensor imaging (DTl) studies of schizophrenia, thus far, have mainly focused on white matter abnormalities reflected in anisotropy indices such as fractional anisotropy (FA), but recently, new implications of apparent diffusion coefficient (ADC) have been proposed. This is. to our knowledge, the first study to analyze ADC maps of schizophrenic Patients using statistical parametric anatomical mapping (SPAM). Methods : DTl was performed in 20 schizophrenic patients and an equal number of age- and sex-matched healthy controls. ADC maps were then generated, from which 98 volumes of interest were extracted using SPAM. Probability-weighted mean values of ADC were calculated from each volume of interest and compared between the two groups. Results : Significantly higher mean ADC values were observed in patients with schizophrenia compared to healthy controls in the following volumes of interest: bilateral hippocampal formation, bilateral uncus, nght thalamus, light temporal lobe white matter, right superior temporal gyrus, bilateral insula and right lateral ventricle. Conclusion : The results support the recently proposed assertion that regionally increased ADC may reflect cortical brain atrophy. In addition, a new possibility is raised that a neuropathological event involving both the gray and the white matter may also play a role.

들잔디 성숙종자로부터 캘러스배양 및 식물체 재분화에 미치는 몇 가지 요인의 영향

이상훈,김범수,원성혜,조진기,김기용,박근제,성병렬,이효신,이병현 Plant molecular biology and biotechnology research 2004 Plant molecular biology and biotechnology research Vol.2004 No.-

In an effort to optimize tissue culture responses of zoysiagrass (Zoysia japonica Steud.) for genetic transformation, factors affecting callus induction and plant regeneration were investigated. MS medium containing 3 ㎎/L 2,4-D was optimal for embryogenic callus induction from mature seed. The plant regeneration frequency of 73.3% was observed when embryogenic calli induced in this medium were transferred to N6 medium supplemented with 0.1 ㎎/L 2,4-D and 5 ㎎/L BA. Among several basic media, MS and N6 medium were optimal for callus induction and plant regeneration, respectively. Regenerated plants were grown normally when shoots transplanted to the soil. A rapid and efficient plant regeneration system established in this study will be useful for molecular breeding of turfgrass through genetic transformation.

Diazepam과 Lidocaine이 노인환자의 기관내삽관시 심전도 및 심맥관계에 미치는 영향

김경희,권성범,신용섭,최세진 충남대학교 의과대학 지역사회의학연구소 1995 충남의대잡지 Vol.22 No.1

We selected at random 30 geriatric patients who had received operation under the general anesthesia with endotracheal intubation. The patients were divided into 3 groups. Group 1 (control) had received endotracheal intubation without diazepam and lidocaine. Group 2 had received endotracheal intubation with diazepam. Group 3 had received endotracheal intubation with lidocaine. The changes of arterial blood pressure and pulse rate, and the appearance of arrhythmia were compared in each group. The results were as follows : 1. The increase of blood pressure and heart rate were less in group 2, 3 than the control group which showed significant increase of blood pressure and heart rate with endotracheal intubation. 2. The incidence of arrhythmias with endotracheal intubation was less in group 2(20%), group 3(50%) than the control group(80%). 3. The electrocardiographic change in the group 2 and 3 were not significant compared to group 1(control).

Sn-3.5wt%Ag 무연솔더를 이용한 μBGA 솔더접합부의 열피로수명 예측

이준환,하범용,신영의 중앙대학교 기술과학연구소 2000 기술과학연구소 논문집 Vol.30 No.-

본 연구에서는 μBGA와 Pb-free 솔더에 대한 수명평가방법을 제시하기 위하여 μBGA에 대하여 37wt%Pb-Sn 공정 솔더와 Sn-3.5wt%Ag 솔더를 채택한 3차원 유한요소해석모델을 구성해 보았다. 점탄소성거동에 대비하여 구성되어진 각각의 모델 중, 먼저 일반적인 솔더 형상을 가진 모델에 대하여 솔더 재질별로 온도범위 -40∼120℃, -20∼100℃, 20∼100℃의 세 가지 열사이클을 3회씩 가하여 솔더 접합부 계면에 집중되어진 응력과 변형율의 분포를 유한요소해석을 통해 살펴보았다. 또한 온도 변화에 따른 ΔT와 용융온도 T_M등의 변수를 이용하여 얻어진 γ를 이용하여 열피로수명식을 유도하였다. This study discusses thermal fatigue prediction of μBGA(Micro Ball Array) solder joint. Analysis method is used to three-dimensional finite element analysis with various solder alloys such as Sn-37wt%Pb, Sn-3.5wt%Ag under temperature cycling. Strain values, along with the result of solder material fatigue tests, were then used to predict the solder joint fatigue life with Coffin-Manson equation. As a result, it could be found that Sn-3.5wt%Ag has longer fatigue life than Sn-37wt%Pb in Low cycle fatigue. And using the analysis result, derive modified Coffin-Manson equation with temperature dependent factor γ.

만화 과제를 이용한 정신분열병 환자의 사회인지 능력의 측정

오정은,나명현,하태현,신용욱,노규식,홍순범,이경진,신민섭,이영호,권준수 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.3

Objectives : This study is to investigate the hypothesis that schizophrenic patients have difficulty in inferring mental state of others. Methods : Twenty schizophrenic patients and twenty normal subjects were administered to the Cartoon Task required inferring the character's intention in a given context. Results : The schizophrenic patients showed a statistically significant impairment in the Cartoon Task. Both groups chose the card depicting a frequent everyday action significantly more rather than the similar card to the last picture in each story for wrong answers. Conclusion : These preliminary results suggest that schizophrenia is associated with a specific deficit of the cognitive ability referred to as social cognition, and this deficit can be detected not only in the acute phase as found in previous research studies, but also in remission. Also when subjects fail to appreciate what is going on in the minds of other people, they seem to rely on a socially familiar experience.

논에서 물과 양분관리에 따른 메탄(CH_4), 아산화질소(N_2O) 배출 특성

김건엽,박상일,송범헌,신용광 한국환경농학회 2002 한국환경농학회지 Vol.21 No.2

물관리방법을 달리한 상시담수와 간단관개에서 메탄과 아산화질소의 배출 양상과 서로 다른 종류의 유기물원 및 질소비료 시용에 따른 메탄과 아산화질소 배출을 비교하고 저감 할 수 있는 방법을 찾기 위해 본 시험을 실시하였다. 물관리방법으로는 상시담수 (이앙∼출수 35일까지 담수)와 이앙 35일 이후 중간낙수한 간단관개 (중간낙수기간 20일),그리고 시비방법으로는 토양을 검정한 시비량인 토양검정, 토양 검정시비량+볏짚 돈분액비, LCU 완효성비료 그리고 N을 시용하지 않은 대조구 등을 처리하여 메탄 및 아산화질소 배출량을 측정하였다. 메탄 배출량은 간단관개보다 상시담수에서 높았고, 아산화질소 배출량은 이와 반대로 나타났다. 유기물과 비료시용 종류에 따른 메탄과 아산화질소 배출량은 액비와 검정시비+볏짚시용구에서 가장 높았으며, LCU 완효성비료구에서 가장 낮았다. 지구온난화잠재력으로 환산한 온실가스 배출은 물관리 조건에서 상시담수가 간단관개보다 170∼208% 높았으며, 요소를 시용하고 상시담수를 기준 한 것과 간단관개로 물 관리하여 각 처리에 따라 온실가스 배출을 비교하면 요소는 41.4%, LCU 완효성비료 55.8%로 온실가스 배출을 줄일 수 있는 것으로 나타났다. 그리고 비료이용효율은 LCU완효성비료, 액비>검정시비+볏짖>검정시비 순이었다. Emission of methane and nitrous oxide affected by nitrogen fertilizer materials were measured simultaneously in rice paddy fields under flooding and intermittent irrigation in 2000. Studies focused on mitigating CH_4 emission from rice paddy fields are summarized and the possibilities and limits applied to world's rice cultivation are discussed. The mitigation options are water management, soil amendments, organic matter management, different tillage, rotation, and cultivar selection. Altering water management, in particular promoting midseason aeration by short-term drainage, is one of the most promising strategies, although these practices may be limited to the rice paddy fields where the irrigation system is well prepared. The test site was divided into two water managements: a continuously flooded plot which was maintained flooded by constant irrigation from May to September, and an intermittently drained plot in which short-term (20days) draining practices were performed one times during the flooding period. By total emission of GHGs converted by global warming potential (GWP), flooding plots were higher 170~208% than intermittent irrigation plots. For emission of GHGs in fertilizer materials, it was high in the order of Swine slurry>Urea+Rice straw>Urea>LCU. Basing on GHGs emission of urea fertilization under flooding as baseline GWP of urea fertilization and Latex-coated urea under intermittent irrigation showed lower GHGs emission by 41.4% and 55.8%, respectively. In this case fertilizer use efficiency (kg unhulled rice/kg of applied N) were 18.2~20.2 and 18.7~19.0 and 9.3 and 5.8~6.6 for Swine slurry and LCU and Urea+Rice straw and Urea in the continuously flooded and intermittently drained plot.

Warfarin과 Baclofen 간의 약물 상호 작용의 검증

고현윤,박재홍,신용범 大韓再活醫學會 1999 Annals of Rehabilitation Medicine Vol.23 No.6

추간판 변성의 영상화: 자기공명영상과추간판 조영술의 비교

고현윤,김훈,이종언,신용범,김학진 大韓再活醫學會 2000 Annals of Rehabilitation Medicine Vol.24 No.1