GC-ECD를 이용한 유기염소계 및 Pyrethroid계 농약 동시 분석법

김우성,이선화,김재이,정지윤,이명자,박영채,이영자,정성욱,이봉헌,박흥재 한국환경과학회 2003 한국환경과학회지 Vol.12 No.4

Pesticides were extracted from samples with 70% acetone and methylene chloride in order, and then cleaned up via open-column chromatography apparatus packed with florisil, and finally analyzed simultaneously the organochlorine and pyrethroid pesticides using GC(ECD). An ultra-2 fused silica capillary column was used to separate and identify the products. The resolution between the last isomeric peak of cypermethrin(59.987min) and the first isomeric peak of flucythrinate(60.043min) was not satisfactory. The last isomeric peak of fenvalerate(62.344min) and the first isomeric peak of fluvalinate(62.397min) were overlapped. Recoveries of soybean sample for the most pesticides were 73.3% to 102.4%. Detection limits were between 0.004 and 0.063 ㎍/㎎ when this method was used.

Metagenomic Analysis of Fungal Communities Inhabiting the Fairy Ring Zone of Tricholoma matsutake

( Miae Kim1 ),( Hyeok Jun Yoon ),( Young Hyun You ),( Ye Eun Kim ),( Ju Ri Woo ),( Yeong Gyo Seo ),( Gyeong Min Lee ),( Young Ja Kim ),( Won Sik Kong ),( Jong Guk Kim ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.10

Tricholoma matsutake, an ectomycorrhiza that has mutual relationships with the rootlet of Pinus denisflora, forms a fruiting body that serves as a valuable food in Asia. However, the artificial culture of this fungus has not been successful. Soil fungi, including T. matsutake, coexist with many other microorganisms and plants; therefore, complex microbial communities have an influence on the fruiting body formation of T. matsutake. Here, we report on the structures of fungal communities associated with the fairy ring of T. matsutake through the pyrosequencing method. Soil samples were collected inside the fairy ring zone, in the fairy ring zone, and outside the fairy ring zone. A total of 37,125 sequencing reads were obtained and 728 to 1,962 operational taxonomic units (OTUs) were observed in the sampling zones. The fairy ring zone had the lowest OTUs and the lowest fungal diversity of all sampling zones. The number of OTUs and fungal taxa inside and outside the fairy ring zone was, respectively, about 2 times and 1.5 times higher than the fairy ring. Taxonomic analysis showed that each sampling zone has different fungal communities. In particular, out of 209 genera total, 6 genera in the fairy ring zone, such as Hemimycena, were uniquely present and 31 genera, such as Mycena, Boletopsis, and Repetophragma, were specifically absent. The results of metagenomic analysis based on the pyrosequencing indicate a decrease of fungal communities in the fairy ring zone and changes of fungal communities depending on the fairy ring growth of T. matsutake.

고등학교 가정과 교육을 위한 교과과정 영역별 필요도

김행자,안영희,이남기,이금남 한국 가정과 교육 학회 1996 한국가정과교육학회지 Vol.8 No.1

This study aims at finding a new home economics education which will include male and female students as its teaching objects, and then providing home economics teachers with useful materials. For this purpose I examined analyzed male and female the upper secondary school students' and parents' recognition and demand to the home economics education. The conclusion of this study is summarized as follows: 1. There was significant difference between male students(73.7%) and female students(89.8%) in the necessity for studying Home Economics(P<.001). In necessity of Home Economics education, male students emphasized the spiritual part but female students emphasized the functional part. In part of parents, there was no significant difference between fathers(95.5%) and mothers(96.4%). 2. The necessity of each part in the contents of Home Economics 1) In the part of family, there was significant difference between male and female students in the true nature of family, the role and responsibility of family, and the laws relating to family(P<.001). There was significant difference between mothers and fathers in the true nature of family and the role and responsibility of family. Fathers and male students emphasized the true nature of family and the role and responsibility of family more than mothers and female students did. 2) In the Home management and economy parts, there was significant difference between male and female students in the significance of home management(P<.001), the relation of family to society(P<.001), and fluctuating measures for home economy. There was significant difference between fathers and mothers in the relation of family to society(P<.05). Fathers and male students emphasized the relation of family to society more than mothers and female students did. Mothers, male and female students emphasized the importance of the protection of consumers. 3) In the clothes part, male students emphasized the role of clothes, the design and making of clothes(P<.001), the present situation of the resources of clothing and the distribution of clothes(P<.05) more than female students did. 5) In the part of housing, male students emphasized home planning, and arrangements and facilities of house more than female students did. Female students emphasized the interior of the house(P<.05). Fathers emphasized the planning of a residential space and arrangements and facilities of a house(P<.05) more than mothers did. 6) In the parts of human development and upbringing, male students emphasized the significance of being parents, family planning and the population problem(P<.001), pregnancy and delivery(P<.01), and the characteristic of juvenile development(P<.05) more than female students did. Parents and students emphasized the importance of prevention and countermeasure for diseases and the significance of being parents.

韓國産 밀어屬漁類(망둑어科)3型의 形態的 特徵

金榮子,田祥麟 상명대학교 자연과학연구소 1996 自然科學硏究 Vol.3 No.-

지식공학을 위한 Prolog 프로그래밍 환경에서 제어 정보에 관한 연구

김자룡,홍영표 진주여자전문대학 1990 論文集 Vol.13 No.-

In the Prolog programming environment it is easy to program by the Pattern compound function and the automatical feed-back device. On the contrary this feed-back device makes debugging difficult bacause user has difficulties in finding out program control. That is, there occurs bug that user doesn't notice error feed-back when performs the undefined predicate. This study proposes box model as debugging tool to control this, and Puts the examples debugging with the devide-and-query to support debug in the status of Algorithm. The devide-and-query is the method to find bug by asking the user whether each clause is correct or not. With this, Prolog, beyond the scope of knowledge representing language, will be able to perform logical interpretation as an Artifical Intelligence Language.

김치가 노화촉진쥐 간의 유리기 생성 및 항산화효소 활성에 미치는 영향

김종현,권명자,이소영,류재두,문갑순,최홍식,송영옥 부산대학교 김치연구소 2002 김치의 과학과 기술 Vol.8 No.-

본 연구는 김치가 노화 억제에 미치는 영향을 살펴보기 위하여 AIN-76마우스 식이에 배추, 갓김치, 갓배추김치를 각 동결건조 김치를 5%씩 첨가하여 만든 후 김치식이를 노화촉진쥐인 SAMP8에게 1년간 섭취시키면서 간의 노화관련 특성인 유리기 농도와 항산화효소계의 효소활성 변화를 살펴 보았다. 노화촉진쥐 간의 총유리기, OH radical, H_2O_2 그리고 TBARS모두 가령에 따라 증가되었으며, 간에서 생성된 유리기 농도와 TBARS 농도는 대조군에 비해 김치섭취시 유의적으로 감소되었다(p<0.05). 그러나 생성된 유리기를 제거하기 위한 SOD활성은 오히려 김치섭취군에서 유의적으로 증가되었으며(p<0.05), catalase와 GSH-px, GSH/GSSG의 비율도 김치섭취군에서 유의적으로 증가하였다(p<0.05). 따라서 김치섭취는 노화에 따라 발생되는 간의 유리기 및 TBARS 생성은 억제시키면서, 이를 제거하는 항산화계 효소는 활성화시켜 노화를 억제한 것으로 생각된다. 김치종류 중에서는 배추김치보다 배추갓김치와 갓김치가 유리기 생성 억제 및 항산화 효소 활성을 더욱 증진시킨 것으로 관찰되어 이는 첨가된 갓의 항산화물질 때문인 것으로 생각된다. This study was performed to investigate the effect of kimchi intake on antiaging characteristics in the brain of senescence-accelerated mouse (SAM) in terms of free radical production and anti-oxidative enzymes. Two hundreds twenty SAM (20 mice) were divided into four groups and fed kimchi diet for 12 months. Experimental groups were kimchi free AIN-76 diet (control) group, Korean cabbage kimchi diet (KCK)group, 30% mustard leaf added Korean cabbage kimchi diet (MKCK) group, and mustard leaf kimchi diet (MLK) group. Concentrations of total free radical, OH radical and H_2O_2 of control group increased up to 123%, 262% and 174% of initial value (p<0.05) after one year. Increase in free radical production in kimchi groups due to aging was decreased by kimchi feeding. Among kimchi groups. MKCK and MLK groups showed greater inhibiting effect against free radical production than KCK. The concentration of TBARS in the bruin of control group also significantly increased up to 362% of initial value as aged (p<0.05) and production of TBARS in kimchi groups were decreased. When the activities of Cu, Zn-SOD, Mn-SOD, GSH-px and catalase of kimchi groups were compared to those of control at the same experimental period, anti-oxidative enzyme activities of kimchi groups were lower than those of control (p<0.05). But GSH/GSSG in kimchi groups were higher compared to control. In conclusion, decrease in free radical production and increase in anti-oxidative enzyme activities were observed from kimchi groups suggesting that kimchi might have important role on retarding aging. Among kimchi variety tested in this experiment, MKCK and MLK seem to have greater effect on inhibiting free radical production and increasing anti-oxidative enzyme activities than KCK. Further study is needed to find out the mechanism of kimchi on retarding aging.

GC/NPD를 이용한 다성분 잔류농약의 동시분석

김우성,이선화,김상엽,정동윤,김재이,이영자,이홍재,정성욱,박흥재 한국환경과학회 2003 한국환경과학회지 Vol.12 No.10

Pesticides were extracted from samples with 70% acetone and methylene chloride in order, and then cleaned up via open-column chromatography apparatus packed with fiorisil, and finally analyzed simultaneously the organophosphorus pesticides using GC/NPD, Ultra-2 and Ultra-1 fused silica capillary columns were used to separate and identify the products. Recovery of most analytes from soybean sample, taken from pesticide residues well, was greater than(80%) for all except(6) analytes. This method can simultaneously determine multiple pesticides with a high degree of accuracy and precision.

어린이 김치 표준화에 대한 연구(1) : 어린이 김치의 담금법 개발 Development of the Preparation of Kimchi for the Children

송영옥,전영수,권명자,빈성미,김은희,문정원,김명 부산대학교 가정대학 1995 家政大學硏究報告 Vol.21 No.-

The purpose of this study is reporting the results of first trial for the standardization of children's kimchi in terms of brining standardization, development for the recipe ad pannel test for the children's kimchi. In order to develop the standard recipe for children's kimchi, our research team have visited kimchi factories, temples and houses located in Pusan and Kyung-Sang Nam Do province. Three kinds of chinese cabbage kimchi (hamyang, haeundae, and jinrae kimchi named by region where we collected) which got the top three highest score in panel testing was selected. The original recipes of these kimchi were sightly modified to prepared the kimchi at laboratory. Among three of them, hamyang kimchi which contained dried anchovy power was selected as a model kimchi for development of the children's kimchi recipe. We find out that the optimal salt concentration of brined cabbage and kimchi for the children's kimchi (sliced style kimchi, approximately 2.5X3cm a piece) that showed the hightest pennel score were approximately 1.5% and 2.0%, respectively. The salt concentration of brined cabbage became 1.5, 1.43, and 1.60% after 60 min at 10% brining solution, 45 min at 12% and 30 min at 15%, respectively. The developed recipe used for the children's kimchi at our team is as follows. The major ingredients used for the preparation of kimchi is brined sliced chinese cabbage (salt concentration is 1.5%) 1kg galic 11g, ginger 5.0g, red pepper powder 20.0g, fermented anchovy juice 25.0g, fermented shrimp juice 5.0g dried anchovy and sea weed extracts 30.0g, anchovy power 2.4g glutinious rice gel 40.0g, starch syrup, 10.0g, and apple juice 22.5g. The total acceptability test of developed children's kimchi (test kimchi) was carried out with 84 of 6th grade student during lunch time at elementary school in Pusan where has a school lunch program. Sixty nine percent of student answered test kimchi is very good, 25% of them said it is fair, and only 6% of students answered they didn't like test kimchil. However, for the kimchi supplied from school at lunch, 52% students answered the kimchi is good, 26% of them said it is fair, and 22% of them said they don't like school supplied kimchi. This result tells us that the test kimchi is better than school supplied kimchi and the test kimchi can be developed further as a children's kimchi.

구조화된 환자교육에 대한 연구논문 분석 : 고혈압 환자를 중심으로 forcused on the patients with hypertension

박청자,이경희,고효정,권영숙,김정남,박영숙 대한보건협회 2003 대한보건연구 Vol.29 No.2

This study was conducted to analyze the nursing research methodology and the key concepts used in articles related hypertension published in the RICH. The purpose of this study was for reflecting the trends of the research on the structured patient education focused the hypertensives. The results were as following. 1. There were 119 research studies related hypertension in RICH from 1994 to 2002. The number increased in 1997. Research studies of 98 have done since 1997 and it is 82% of the total research studies. 2. The most of the research studies are non-degree research studies. Research studies with fund have been rapidly increased in 2000 year. 3. The prevailing research design were the non-experimental design, quasi-experimental and pre-experimental designs in order. 4. The data collection method used most often physiologic measures(32.8%). 5. The domain of the key concepts that prevailed was health domain(51.8%), nursing domain(22.9%), and human being domain(20.5%) in order. And environmental domain (4.8%) were relatively low.

주의력결핍-과잉행동 아동과 관련된 문제행동 특성

윤정자,김영희 충북대학교 교육·생활연구소 1999 생활과학연구논총 Vol.2 No.-

The purpose of present study was to identify the associated probelm behaviors of Attention-Deficit Hyperactivity Disorder (ADHD) children and to examine the differences in probelm behaviors across situation. The subjects of survey research consisted of 228 preschool children(147 boys and 136 girls aged from 3- to 7-year-old) drawn from five Child Care Centers in Chung-Ju. Data were analyzed by the frequency, percentages, Cronbach α coefficient, F-test, Tukey post-hoc test, and Pearson correlation using SAS program. The results were as followings: ADHD was highly correlated with externalized problem behaviors such as oppositional, immatural, and aggressive behaviors. It also was significantly associated with internalized problem behavior such as emotional instability. Correlations between ADHD and other problem behaviors were from .14 through .63. There were significant differences in problem behaviors across situation. Among groups, ADHD-School group was more likely to link to problem behaviors than other groups. Children of pervasive ADHD groups were more likely to have externalized behaviors than other groups.