Cornin increases angiogenesis and improves functional recovery after stroke via the Ang1/Tie2 axis and the Wnt/b-catenin pathway

Yangyang Xu,Guanghua Zhang,Zechun Kang,Yong Xu,Wangling Jiang,Shuping Zhang 대한약학회 2016 Archives of Pharmacal Research Vol.39 No.1

We investigated whether cornin, an iridoid glycoside isolated from fruits of Verbena officinalis L., regulated angiogenesis and thereby improved functional outcomes after stroke and discovered a potential mechanism. The effects of cornin on proliferation of rat artery smooth muscle cell (RASMC) and signalling was investigated in vitro. Adult male rats were subjected to 1 h of middle cerebral artery occlusion (MCAO) and reperfusion and treated with or without 25 mg/kg of cornin, starting 24 h after ischemia and reperfusion, by continuous intravenous injection daily for 14 days. Neurological functional tests were performed and cerebral Evans blue extravasation was measured. Angiogenesis and angiogenic factor expressions were measured by immunohistochemistry and Western blotting, respectively. Cornin increased the proliferation of RASMC and enhanced the expression of Wnt5a, b-catenin, cyclin D1 and angiopoietin-1 (Ang1). Cornin treatment promoted angiogenesis in the ischemic brain core and improved functional outcomes after stroke. Cornin-treated MCAO rats showed significant increase in vascularization and expression of vascular endothelial growth factor and Ang1 and phosphorylation of Tie2 and Akt compared with vehicle-treated MCAO rats. The Ang1/ Tie2 axis and Wnt/b-catenin pathways appear to mediate cornin-induced angiogenesis.

Candidate Pruning-Based Differentially Private Frequent Itemsets Mining

Yangyang Xu,Zhaobin Liu,Zhonglian Hu,Zhiyang Li 보안공학연구지원센터 2016 International Journal of Database Theory and Appli Vol.9 No.7

Frequent Itemsets Mining(FIM) is a typical data mining task and has gained much attention. Due to the consideration of individual privacy, various studies have been focusing on privacy-preserving FIM problems. Differential privacy has emerged as a promising scheme for protecting individual privacy in data mining against adversaries with arbitrary background knowledge. In this paper, we present an approach to exploring frequent itemsets under rigorous differential privacy model, a recently introduced definition which provides rigorous privacy guarantees in the presence of arbitrary external information. The main idea of differentially privacy FIM is perturbing the support of item which can hide changes caused by absence of any single item. The key observation is that pruning the number of unpromising candidate items can effectively reduce noise added in differential privacy mechanism, which can bring about a better tradeoff between utility and privacy of the result. In order to effectively remove the unpromising items from each candidate set, we use a progressive sampling method to get a super set of frequent items, which is usually much smaller than the original item database. Then the sampled set will be used to shrink candidate set. Extensive experiments on real data sets illustrate that our algorithm can greatly reduce the noise scale injected and output frequent itemsets with high accuracy while satisfying differential privacy.

CDH17 nanobodies facilitate rapid imaging of gastric cancer and efficient delivery of immunotoxin

Jingbo Ma,Xiaolong Xu,Chunjin Fu,Peng Xia,Ming Tian,Liuhai Zheng,Kun Chen,Xiaolian Liu,Yilei Li,Le Yu,Qinchang Zhu,Yangyang Yu,Rongrong Fan,Haibo Jiang,Zhifen Li,Chuanbin Yang,Chengchao Xu,Ying Long,J 한국생체재료학회 2022 생체재료학회지 Vol.26 No.4

Background: It is highly desirable to develop new therapeutic strategies for gastric cancer given the low survival rate despite improvement in the past decades. Cadherin 17 (CDH17) is a membrane protein highly expressed in cancers of digestive system. Nanobody represents a novel antibody format for cancer targeted imaging and drug delivery. Nanobody targeting CHD17 as an imaging probe and a delivery vehicle of toxin remains to be explored for its theragnostic potential in gastric cancer. Methods: Naïve nanobody phage library was screened against CDH17 Domain 1-3 and identified nanobodies were extensively characterized with various assays. Nanobodies labeled with imaging probe were tested in vitro and in vivo for gastric cancer detection. A CDH17 Nanobody fused with toxin PE38 was evaluated for gastric cancer inhibition in vitro and in vivo. Results: Two nanobodies (A1 and E8) against human CDH17 with high affinity and high specificity were successfully obtained. These nanobodies could specifically bind to CDH17 protein and CDH17-positive gastric cancer cells. E8 nanobody as a lead was extensively determined for tumor imaging and drug delivery. It could efficiently co-localize with CDH17-positive gastric cancer cells in zebrafish embryos and rapidly visualize the tumor mass in mice within 3 h when conjugated with imaging dyes. E8 nanobody fused with toxin PE38 showed excellent anti-tumor effect and remarkably improved the mice survival in cell-derived (CDX) and patient-derived xenograft (PDX) models. The immunotoxin also enhanced the anti-tumor effect of clinical drug 5-Fluorouracil. Conclusions: The study presents a novel imaging and drug delivery strategy by targeting CDH17. CDH17 nanobodybased immunotoxin is potentially a promising therapeutic modality for clinical translation against gastric cancer.

HALF-METALLIC SILICENE AND GERMANENE NANORIBBONS: TOWARDS HIGH-PERFORMANCE SPINTRONICS DEVICE

YANGYANG WANG,JIAXIN ZHENG,ZEYUAN NI,RUIXIANG FEI,QIHANG LIU,RUGE QUHE,CHENGYONG XU 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2012 NANO Vol.7 No.5

By using first-principles calculations, we predict that an in-plane homogenous electrical ¯eld can induce half-metallicity in hydrogen-terminated zigzag silicene and germanene nanoribbons (ZSiNRs and ZGeNRs). A dual-gated ¯nite ZSiNR device reveals a nearly perfect spin-¯lter e±ciency (SFE)of up to 99% while a quadruple-gated ¯nite ZSiNR device serves as an effective spin ¯eld e®ect transistor (FET) with an on/o® current ratio of over 100 from ab initio quantum transport simulation. This discovery opens up novel prospect of silicene and germanene in spintronics.

Corrosion inhibition properties of two imidazolium ionic liquids with hydrophilic tetrafluoroborate and hydrophobic hexafluorophosphate anions in acid medium

Yangyang Guo,Bin Xu,Ying Liu,Wenzhong Yang,Xiaoshuang Yin,Yun Chen,Jinxun Le,Zhihao Chen 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.56 No.-

The ionic liquids, 1-vinyl-3-aminopropylimidazolium hexafluorophosphate ([VAIM][PF6]) and 1-vinyl-3-aminopropylimidazolium tetrafluoroborate ([VAIM][BF4]) acted as the corrosion inhibitors. Weight lossmeasurements showed the corrosion inhibition efficiencies of [VAIM][PF6] and [VAIM][BF4] were 90.53%and 54.01% at 45 C, respectively. The ILs were mix-type inhibitors. Raising the temperature can have adecreased inhibition efficiency of [VAIM][BF4], while [VAIM][PF6] presented an opposite trend. Inaddition, [VAIM][PF6] obeyed Langmuir monolayer adsorption isotherm, while [VAIM][BF4] obeyed EIAwadykinetic-thermodynamic adsorption for blocking active sites. Moreover, Molecular DynamicSimulations showed that [VAIM][PF6] owned a higher adsorption energy, which may be responsible forthe more adsorption groups of [VAIM][PF6].

A Novel Frequency Characteristic Model and Noise Shaping Method for Encoder-Based Speed Measurement in Motor Drive

Yangyang Chen,Ming Yang,Dianguo Xu,Frede Blaabjerg 전력전자학회 2019 ICPE(ISPE)논문집 Vol.2019 No.5

The performance of the closed-loop control system is determined by the accuracy of the feedback signal to some extent. In most motor drive systems, the position and speed signals come from the optical encoder. Thus, to reduce the quantization error from encoder and achieve better speed measurement results are the focuses of scholars and engineers. Although many improved speed measurement methods have been proposed, there are still few literatures discussed about the frequency characteristic of the encoder quantization noise. Even in some algorithms like Kalman filter based speed measurement methods usually regard the quantization noise as white noise. In this paper, a noise frequency characteristic model of encoder-based speed measurement in motor drive is built up. According to this model, the variation rule of the noise frequency is found. Besides, a variable-period noise shaping method is proposed to reduce the impact of quantization noise. Both the notch filter and a finite impulse response (FIR) filter are used to cope with the quantization noise. The frequency model and noise shaping method are verified by experiment results.

A New Theory for Acupuncture: Promoting Robust Regulation

Yuan Xu,Yang Guo,Yuru Song,Kuo Zhang,Yiping Zhang,Qiang Li,Shouhai Hong,Yangyang Liu,Yi Guo 사단법인약침학회 2018 Journal of Acupuncture & Meridian Studies Vol.11 No.1

Robustness, an ability of biological networks to uphold their functionalities in the face of perturbations, is a key characteristic of all living systems. Acupuncture is a procedure in which fine needles are inserted into an individual at discrete points and then manipulated, with the intent of preventing and curing diseases. Acupuncture does not directly eliminate pathogenic factors or pathological tissue; rather, acupuncture enhances the ability of the human body to self-medicate itself by activating complex regulatory systems and by maintaining physiological homeostasis to prevent or treat diseases. From this point of view, the effect of acupuncture on the human body is more likely a kind of regulation to promote robustness. That is to say, acupuncture has the ability to promote robustness. In this article, we review the properties and functions of acupuncture in preventing and treating diseases and in maintaining health by enhancing robustness.

Comparative experimental study of ethanol-air premixed laminar combustion characteristics by laser induced spark and electric spark ignition

Cangsu Xu,Yangyang Hu,Xiaolu Li,Xuan Zhou,Anhao Zhong 한국화학공학회 2017 Korean Journal of Chemical Engineering Vol.34 No.2

An experimental study of laminar combustion characteristics of ethanol-air premixed mixtures was conducted with different ignition methods, including laser induced spark ignition (LISI) and electric spark ignition (SI) at an initial condition of 358 K temperature and 0.1MPa pressure. Flame propagation with the two different ignition methods was analyzed and discussed. The laminar flame speed was extrapolated with a nonlinear extrapolation method. Results indicate that the laminar speed of ethanol-air mixtures with LISI is faster than that with SI at lean mixtures, but slower at stoichiometric and rich mixtures. The peak values of the laminar burning velocity for SI and LISI with nonlinear extrapolation are 50.1 cm/s and 47.6 cm/s at the equivalence ratio of 1.1, respectively. Laser-induced spark ignition is able to ignite leaner ethanol-air mixtures.

How to evaluate the adequacy of staging for nodal-negative epithelial ovarian cancer? Use of nodal staging score

Yuan Xu,Haoran Li,Xiaoxia Tong,Yangyang Pang,Xiaojuan Tong,Luhong Li,Lei Cheng 대한부인종양학회 2019 Journal of Gynecologic Oncology Vol.30 No.2

Objective: No guideline has been provided to assess the minimal number of lymph nodes (LNs) that should be dissected for accurate staging in patients with epithelial ovarian cancer (EOC). The aim of the study was to develop a nodal staging score (NSS) as an index to assess whether a pathologic (p)N0 EOC patient is indeed free of a nodal disease. Methods: A total of 16,361 EOC patients staged I–III between 2004 and 2013 were identified from the Surveillance, Epidemiology and End Result database. With a β-binomial model, NSS was calculated to assess the probability of true-negative findings of LN status. Results: With an increased number of LNs examined, the probability of missing a nodal disease decreased and varied among different pT stages. Given 1 LN examined, an NSS of 93.76% calculated could ensure a high adequacy of nodal-negative classification for pT1N0 EOC patients. For pT2N0 patients, 5 LNs examined could guarantee an NSS of 90% for adequate staging. Likewise, 11 and 29 LNs examined in pT3N0 patients could maintain NSS at the level of 80% and 90%, respectively. Our study suggested the optimal number of LNs that could be examined and stratified by the pT stages for EOC patients based on this statistical model derived from large pathologic data of clinical surgery patients. Conclusion: NSS, as an auxiliary tool, not only could assist the International Federation of Gynecology and Obstetrics staging more precisely, but also would provide a statistical basis for postoperative evaluation for further clinical decision-making.

Study on fracture characteristics of reinforced concrete wedge splitting tests

Hu Shaowei,XU Aiqing,HU Xin,YIN Yangyang 사단법인 한국계산역학회 2016 Computers and Concrete, An International Journal Vol.18 No.3

To study the influence on fracture properties of reinforced concrete wedge splitting test specimens by the addition of reinforcement, and the restriction of steel bars on crack propagation, 7 groups reinforced concrete specimens of different reinforcement position and 1 group plain concrete specimens with the same size factors were designed and constructed for the tests. Based on the double-K fracture criterion and tests, fracture toughness calculation model which was suitable for reinforced concrete wedge splitting tensile specimens has been obtained. The results show that: the value of initial craking load Pini and unstable fracture load Pun decreases gradually with the distance of reinforcement away from specimens’s top. Compared with plain concrete specimens, addition of steel bar can reduce the value of initial fracture toughness KIini, but significantly increase the value of the critical effective crack length ac and unstable fracture toughness KIun. For tensional concrete member, the effect of anti-cracking by reinforcement was mainly acted after cracking, the best function of preventing fracture initiation was when the steel bar was placed in the middle of the crack, and when the reinforcement was across the crack and located away from crack tip, it plays the best role in inhibiting the extension of crack.