Comparative analysis of metabolites and in vitro hypoglycemic activity of Taiwanofungus camphoratus cultured using various methods

Ming YongFei,Li Yin,Chu JianZhi,Zhou XiaoShuang,Huang YuXuan,Yang ShuDe,Mu YueJun,Wang Lin,Zhang Rui,Cheng XianHao 한국응용생명화학회 2024 Applied Biological Chemistry (Appl Biol Chem) Vol.67 No.-

Taiwanofungus camphoratus has attracted much attention because it can abundantly produce various active substances that exhibit blood-sugar lowering, immunity improving, and antioxidant properties. Currently, T. camphoratus is cultured using four main methods: cutting wood culture, solid-state fermentation, submerged fermentation, and dish culture. T. camphoratus produces different metabolites under different culture methods. In this study, nontargeted metabolomics was used to compare the metabolites of T. camphoratus produced under these four culture methods. Principal component analysis and supervised partial least squares-discriminant analysis were used to analyze the differences in the metabolites. Moreover, in vitro hypoglycemic activity of T. camphoratus extracts produced under four culture methods was compared by assessing their ability to inhibit the activity of α-glucosidase, α-amylase, and sucrase. A total of 186 metabolites were identified. In total, 127 metabolites were common under the four culture methods. Under solid-state fermentation, submerged fermentation, and cutting wood culture, 12, 1, and 4 metabolites were unique, respectively. The differential metabolites produced by T. camphoratus under four culture methods were mainly triterpenoids, phenolic compounds, and fatty acid compounds. α-glucosidase, α-amylase, and sucrase activity inhibition was the best using T. camphoratus extract obtained under cutting wood culture; the inhibition rates were 55.97%, 51.96%, and 78.02%, respectively, which were comparable to those exhibited by 0.001, 3, and 12 mg/mL acarbose (positive control). The metabolites produced by T. camphoratus and α-glucosidase, α-amylase, and sucrase inhibitory activities were different under the four culture methods. Cutting wood culture exhibited the best enzyme inhibitory activity. This study provided a theoretical basis for further use and development of various culture methods for the rational production of active metabolites of T. camphoratus . Taiwanofungus camphoratus has attracted much attention because it can abundantly produce various active substances that exhibit blood-sugar lowering, immunity improving, and antioxidant properties. Currently, T. camphoratus is cultured using four main methods: cutting wood culture, solid-state fermentation, submerged fermentation, and dish culture. T. camphoratus produces different metabolites under different culture methods. In this study, nontargeted metabolomics was used to compare the metabolites of T. camphoratus produced under these four culture methods. Principal component analysis and supervised partial least squares-discriminant analysis were used to analyze the differences in the metabolites. Moreover, in vitro hypoglycemic activity of T. camphoratus extracts produced under four culture methods was compared by assessing their ability to inhibit the activity of α-glucosidase, α-amylase, and sucrase. A total of 186 metabolites were identified. In total, 127 metabolites were common under the four culture methods. Under solid-state fermentation, submerged fermentation, and cutting wood culture, 12, 1, and 4 metabolites were unique, respectively. The differential metabolites produced by T. camphoratus under four culture methods were mainly triterpenoids, phenolic compounds, and fatty acid compounds. α-glucosidase, α-amylase, and sucrase activity inhibition was the best using T. camphoratus extract obtained under cutting wood culture; the inhibition rates were 55.97%, 51.96%, and 78.02%, respectively, which were comparable to those exhibited by 0.001, 3, and 12 mg/mL acarbose (positive control). The metabolites produced by T. camphoratus and α-glucosidase, α-amylase, and sucrase inhibitory activities were different under the four culture methods. Cutting wood culture exhibited the best enzyme inhibitory activity. This study provided a theoretical basis for further use and development of various culture methods for the rational production of active metabolites of T. camphoratus.

Scale inhibition performance of calcium sulfate by 1,6-diaminohexanecontained polyaminoamide dendrimers: Static experiment and MD simulation

Yue Sun,Li Li,Zhihao Chen,Xiaoshuang Yin,Wenzhong Yang,Yun Chen,Ying Liu 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.115 No.-

The calcium sulfate scale is a common scale in the industrial water system, which is difficult to beremoved by common chemical cleaning methods. In this paper, a new phosphorous free of 1,6-Diaminohexane-contained polyaminoamide dendrimers (PAMAM) scale inhibitor with a threedimensional(3D) branched structure has been developed. The scale inhibition performances of differentgenerations of PAMAM dendrimers (PAMAM-mid, PAMAM-0G, PAMAM-0.5G and PAMAM-1G) are estimatedby the static scale inhibition method. Moreover, the effects of the scale inhibitor concentrationsand solution temperatures on the CaSO4 scale inhibition efficiency are thoroughly discussed. Results indicatethat the PAMAM-mid and PAMAM-0.5G) exhibit excellent inhibition performance on CaSO4 precipitation. Especially, the CaSO4 scale inhibition efficiency of PAMAM-0.5G is above 95% when the dose is10 mg/L, and the corresponding value is almost 100% when the inhibitor dose is 20 mg/L. In addition,the influence of descaling agents on the crystal and morphology of CaSO4 scales are studied by XRDand SEM tests. The results show that the scale inhibition ability of the PAMAM molecules mainly inhibitsthe growth of these crystal planes by adsorption on the surface of the growing crystal. Molecular dynamicssimulation results find that the PAMAM-0.5G dendrimer molecule can energetically interact well withthree crystal planes of calcium sulfate dihydrate. Finally, the simulation results provide a theoreticalguidance to judge the performance of scale inhibitors and synthesize new high-efficiency scale inhibitor.

Corrosion inhibition properties of two imidazolium ionic liquids with hydrophilic tetrafluoroborate and hydrophobic hexafluorophosphate anions in acid medium

Yangyang Guo,Bin Xu,Ying Liu,Wenzhong Yang,Xiaoshuang Yin,Yun Chen,Jinxun Le,Zhihao Chen 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.56 No.-

The ionic liquids, 1-vinyl-3-aminopropylimidazolium hexafluorophosphate ([VAIM][PF6]) and 1-vinyl-3-aminopropylimidazolium tetrafluoroborate ([VAIM][BF4]) acted as the corrosion inhibitors. Weight lossmeasurements showed the corrosion inhibition efficiencies of [VAIM][PF6] and [VAIM][BF4] were 90.53%and 54.01% at 45 C, respectively. The ILs were mix-type inhibitors. Raising the temperature can have adecreased inhibition efficiency of [VAIM][BF4], while [VAIM][PF6] presented an opposite trend. Inaddition, [VAIM][PF6] obeyed Langmuir monolayer adsorption isotherm, while [VAIM][BF4] obeyed EIAwadykinetic-thermodynamic adsorption for blocking active sites. Moreover, Molecular DynamicSimulations showed that [VAIM][PF6] owned a higher adsorption energy, which may be responsible forthe more adsorption groups of [VAIM][PF6].

Fabrication and characterization of polypyrrole coatings by embedding antimony modified SnO2 nanoparticles

Zhihao Chen,Yijie Jin,Wenzhong Yang,Bin Xu,Yun Chen,Xiaoshuang Yin,Ying Liu 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.75 No.-

Polypyrrole (PPy) coatings were successfully electrosynthesized on the carbon steel by embedding SnO2and Sb-SnO2 nanoparticles (NPs). The associated structural, morphology, hydrophobic, physicalproperties, anti-corrosion and self-healing performance of these coatings were systematically studiedby several tests. XRD results showed that SnO2 had a tetragonal rutile structure and the lattice parametersindicated that Sb ions were successful substituted into SnO2 lattice. The average size of SnO2 NPs wasdecreased by the incorporation of Sb-SnO2 NPs, which were found through TEM images. The contactangle test showed that the hydrophobic of PPy coatings was greatly enhanced by the incorporation of Sb-SnO2 NPs. SEM results revealed that Sb-SnO2/PPy coating exhibited a more compact and uniform surfacethan SnO2/PPy coating, hence the physical barrier effect was significantly strengthened. Theelectrochemical measurements confirmed the excellent anti-corrosion and self-healing ability of Sb-SnO2/PPy coating for a long immersion time in HCl solution. The superior anti-corrosion performance ofSb-SnO2/PPy coating was ascribed to the synergistic effects of the anodic protection combined with theself-healing effect, the excellent physical barrier effect with compact, smooth and hydrophobic coatingsurface as well as the formation of p-n junction with decreased charge transfer.