MiRNA320a Inhibitor-Loaded PLGA-PLL-PEG Nanoparticles Contribute to Bone Regeneration in Trauma-Induced Osteonecrosis Model of the Femoral Head

Zhang Ying,Li Chuan,Wei Qiushi,Yuan Qiang,He Wei,Zhang Ning,Dong Yiping,Jing Zhenhao,Zhang Leilei,Wang Haibin,Cao Xiangyang 한국조직공학과 재생의학회 2024 조직공학과 재생의학 Vol.21 No.1

BACKGROUND: This study aimed to explore the effect of a nanomaterial-based miR-320a inhibitor sustained release system in trauma-induced osteonecrosis of the femoral head (TIONFH). METHODS: The miR-320a inhibitor-loaded polyethylene glycol (PEG)- Poly(lactic-co-glycolic acid) (PLGA)- Poly-L-lysine (PLL) nanoparticles were constructed using the double emulsion method. The TIONFH rabbit model was established to observe the effects of miR-320a inhibitor nanoparticles in vivo. Hematoxylin–eosin staining and microcomputed tomography scanning were used for bone morphology analysis. Bone marrow mesenchymal stem cells (BMSCs), derived from TIONFH rabbits, were used for in vitro experiments. Cell viability was determined using the MTT assay. RESULTS: High expression of miR-320a inhibited the osteogenic differentiation capacity of BMSCs in vitro by inhibiting the expression of the osteoblastic differentiation markers ALP and RUNX2. MiR-320a inhibitor-loaded PEG-PLGA-PLL nanoparticles were constructed with a mean loading efficiency of 1.414 ± 0.160%, and a mean encapsulation efficiency of 93.45 ± 1.24%, which released 50% of the loaded miR-320a inhibitor at day 12 and 80% on day 18. Then, inhibitor release entered the plateau. After treatment with the miR-320a inhibitor nanoparticle, the empty lacunae were decreased in the femoral head tissue of TIONFH rabbits, and the osteoblast surface/bone surface (Ob.S/BS), osteoblast number/bone perimeter (Ob.N/B.Pm), bone volume fraction, and bone mineral density increased. Additionally, the expression of osteogenic markers RUNX2 and ALP was significantly elevated in the TIONFH rabbit model. CONCLUSION: The miR-320a inhibitor-loaded PEG-PLGA-PLL nanoparticle sustained drug release system significantly contributed to bone regeneration in the TIONFH rabbit model, which might be a promising strategy for the treatment of TIONFH. BACKGROUND: This study aimed to explore the effect of a nanomaterial-based miR-320a inhibitor sustained release system in trauma-induced osteonecrosis of the femoral head (TIONFH). METHODS: The miR-320a inhibitor-loaded polyethylene glycol (PEG)- Poly(lactic-co-glycolic acid) (PLGA)- Poly-L-lysine (PLL) nanoparticles were constructed using the double emulsion method. The TIONFH rabbit model was established to observe the effects of miR-320a inhibitor nanoparticles in vivo. Hematoxylin–eosin staining and microcomputed tomography scanning were used for bone morphology analysis. Bone marrow mesenchymal stem cells (BMSCs), derived from TIONFH rabbits, were used for in vitro experiments. Cell viability was determined using the MTT assay. RESULTS: High expression of miR-320a inhibited the osteogenic differentiation capacity of BMSCs in vitro by inhibiting the expression of the osteoblastic differentiation markers ALP and RUNX2. MiR-320a inhibitor-loaded PEG-PLGA-PLL nanoparticles were constructed with a mean loading efficiency of 1.414 ± 0.160%, and a mean encapsulation efficiency of 93.45 ± 1.24%, which released 50% of the loaded miR-320a inhibitor at day 12 and 80% on day 18. Then, inhibitor release entered the plateau. After treatment with the miR-320a inhibitor nanoparticle, the empty lacunae were decreased in the femoral head tissue of TIONFH rabbits, and the osteoblast surface/bone surface (Ob.S/BS), osteoblast number/bone perimeter (Ob.N/B.Pm), bone volume fraction, and bone mineral density increased. Additionally, the expression of osteogenic markers RUNX2 and ALP was significantly elevated in the TIONFH rabbit model. CONCLUSION: The miR-320a inhibitor-loaded PEG-PLGA-PLL nanoparticle sustained drug release system significantly contributed to bone regeneration in the TIONFH rabbit model, which might be a promising strategy for the treatment of TIONFH.

Temperature-Dependent Photovoltaic Characterization of a CdTe/CdSe Nanocrystal’s Solar Cell

Huichao Zhang,Zhengyang Li,Jun Qian,Qiumei Guan,Xiaowei Du,Yiping Cui,Jiayu Zhang 대한금속·재료학회 2014 ELECTRONIC MATERIALS LETTERS Vol.10 No.2

An all-inorganic thin film solar cell was fabricated with colloidal CdTe and CdSe nanocrystals. Its temperaturedependent photovoltaic characterization was measured, and an open circuit voltage decay technique was used to determine the electron lifetime. The photovoltaic parameters, such as the exact carrier lifetime, were remarkably different between under low-temperature region and under temperature above 267 K. It is suggested that the temperature-dependent carrier conductivity results from a thermally-activated hopping process and this nanocrystals device is a kind of donor-acceptor solar cell.

Effects of intermittent chemical dosing on volatile sulfur compounds in sewer headspace

Guijiao Zhang,Tianfeng Gu,Yongchao Zhou,David Z. Zhu,Yiping Zhang 대한환경공학회 2022 Environmental Engineering Research Vol.27 No.3

Volatile sulfur compounds (VSCs), including hydrogen sulfide (H₂S) and volatile organic sulfide compounds (VOSCs), can be produced in sewer systems causing sewer odor problems. In this study, the effects of intermittently dosing ferric iron, hydrogen peroxide, and nitrate on H₂S and VOSCs in sewer headspace were investigated. In order to characterize the composition of VSCs, an HC-3 trace sulfur analyzer and gas chromatograph (GC) equipped with a triple quadrupole-type mass spectrometry (MS) apparatus were used to determine the VSCs. The results indicated that the effect of intermittent addition of 40 mg/L ferric iron or 40 mg/L hydrogen peroxide is limited for VSCs inhibition. The H₂S and VOSCs concentrations increased significantly in the late-stage experiments, even around 20% and 30% respectively higher than the initial average concentrations. However, the intermittent addition of 40 mg N/L nitrate has a relatively stable control effect of H₂S and VOSCs which maintaining 60% removal rate. Moreover, methyl mercaptan (MeSH) was the most abundant compound of the total VOSCs released and the results of the theoretical odor concentration study also indicate MeSH is the main VOSC causing the significant odor problem. Therefore, more attention should be focused on the VOSCs which have extremely low odor threshold.

Identification of Actinobacillus pleuropneumoniae Genes Preferentially Expressed During Infection Using In Vivo-Induced Antigen Technology (IVIAT)

Fei Zhang,Yangyi Zhang,Xintian Wen,Xiaobo Huang,Yiping Wen,Rui Wu,Qigui Yan,Yong Huang,Xiaoping Ma,Qin Zhao,Sanjie Cao 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.10

Host Langerin (CD207) is a receptor for <i>Yersinia pestis</i> phagocytosis and promotes dissemination

Yang, Kun,Park, Chae G,Cheong, Cheolho,Bulgheresi, Silvia,Zhang, Shusheng,Zhang, Pei,He, Yingxia,Jiang, Lingyu,Huang, Hongping,Ding, Honghui,Wu, Yiping,Wang, Shaogang,Zhang, Lin,Li, Anyi,Xia, Lianxu,B Nature Publishing Group 2015 Immunology and Cell Biology Vol. No.

<P><I>Yersinia pestis</I> is a Gram‐negative bacterium that causes plague. After <I>Y. pestis</I> overcomes the skin barrier, it encounters antigen‐presenting cells (APCs), such as Langerhans and dendritic cells. They transport the bacteria from the skin to the lymph nodes. However, the molecular mechanisms involved in bacterial transmission are unclear. Langerhans cells (LCs) express Langerin (CD207), a calcium‐dependent (C‐type) lectin. Furthermore, <I>Y. pestis</I> possesses exposed core oligosaccharides. In this study, we show that <I>Y. pestis</I> invades LCs and Langerin‐expressing transfectants. However, when the bacterial core oligosaccharides are shielded or truncated, <I>Y. pestis</I> propensity to invade Langerhans and Langerin‐expressing cells decreases. Moreover, the interaction of <I>Y. pestis</I> with Langerin‐expressing transfectants is inhibited by purified Langerin, a DC‐SIGN (DC‐specific intercellular adhesion molecule 3 grabbing nonintegrin)‐like molecule, an anti‐CD207 antibody, purified core oligosaccharides and several oligosaccharides. Furthermore, covering core oligosaccharides reduces the mortality associated with murine infection by adversely affecting the transmission of <I>Y. pestis</I> to lymph nodes. These results demonstrate that direct interaction of core oligosaccharides with Langerin facilitates the invasion of LCs by <I>Y. pestis</I>. Therefore, Langerin‐mediated binding of <I>Y. pestis</I> to APCs may promote its dissemination and infection.</P>

Polymorphisms in the Perilipin Gene May Affect Carcass Traits of Chinese Meat-type Chickens

Zhang, Lu,Zhu, Qing,Liu, Yiping,Gilbert, Elizabeth R.,Li, Diyan,Yin, Huadong,Wang, Yan,Yang, Zhiqin,Wang, Zhen,Yuan, Yuncong,Zhao, Xiaoling Asian Australasian Association of Animal Productio 2015 Animal Bioscience Vol.28 No.6

Improved meat quality and greater muscle yield are highly sought after in high-quality chicken breeding programs. Past studies indicated that polymorphisms of the Perilipin gene (PLIN1) are highly associated with adiposity in mammals and are potential molecular markers for improving meat quality and carcass traits in chickens. In the present study, we screened single nucleotide polymorphisms (SNPs) in all exons of the PLIN1 gene with a direct sequencing method in six populations with different genetic backgrounds (total 240 individuals). We evaluated the association between the polymorphisms and carcass and meat quality traits. We identified three SNPs, located on the 5' flanking region and exon 1 of PLIN1 on chromosome 10 (rs315831750, rs313726543, and rs80724063, respectively). Eight main haplotypes were constructed based on these SNPs. We calculated the allelic and genotypic frequencies, and genetic diversity parameters of the three SNPs. The polymorphism information content (PIC) ranged from 0.2768 to 0.3750, which reflected an intermediate genetic diversity for all chickens. The CC, CT, and TT genotypes influenced the percentage of breast muscle (PBM), percentage of leg muscle (PLM) and percentage of abdominal fat at rs315831750 (p<0.05). Diplotypes (haplotype pairs) affected the percentage of eviscerated weight (PEW) and PBM (p<0.05). Compared with chickens carrying other diplotypes, H3H7 had the greatest PEW and H2H2 had the greatest PBM, and those with diplotype H7H7 had the smallest PEW and PBM. We conclude that PLIN1 gene polymorphisms may affect broiler carcass and breast muscle yields, and diplotypes H3H7 and H2H2 could be positive molecular markers to enhance PEW and PBM in chickens.

Polymorphisms in the Perilipin Gene May Affect Carcass Traits of Chinese Meat-type Chickens

Lu Zhang,Qing Zhu,Yiping Liu,Elizabeth R. Gilbert,Diyan Li,Huadong Yin,Yan Wang,Zhiqin Yang,Zhen Wang,Yuncong Yuan,Xiao-Ling Zhao 아세아·태평양축산학회 2015 Animal Bioscience Vol.28 No.6

Improved meat quality and greater muscle yield are highly sought after in high-quality chicken breeding programs. Past studies indicated that polymorphisms of the Perilipin gene (PLIN1) are highly associated with adiposity in mammals and are potential molecular markers for improving meat quality and carcass traits in chickens. In the present study, we screened single nucleotide polymorphisms (SNPs) in all exons of the PLIN1 gene with a direct sequencing method in six populations with different genetic backgrounds (total 240 individuals). We evaluated the association between the polymorphisms and carcass and meat quality traits. We identified three SNPs, located on the 5′ flanking region and exon 1 of PLIN1 on chromosome 10 (rs315831750, rs313726543, and rs80724063, respectively). Eight main haplotypes were constructed based on these SNPs. We calculated the allelic and genotypic frequencies, and genetic diversity parameters of the three SNPs. The polymorphism information content (PIC) ranged from 0.2768 to 0.3750, which reflected an intermediate genetic diversity for all chickens. The CC, CT, and TT genotypes influenced the percentage of breast muscle (PBM), percentage of leg muscle (PLM) and percentage of abdominal fat at rs315831750 (p<0.05). Diplotypes (haplotype pairs) affected the percentage of eviscerated weight (PEW) and PBM (p<0.05). Compared with chickens carrying other diplotypes, H3H7 had the greatest PEW and H2H2 had the greatest PBM, and those with diplotype H7H7 had the smallest PEW and PBM. We conclude that PLIN1 gene polymorphisms may affect broiler carcass and breast muscle yields, and diplotypes H3H7 and H2H2 could be positive molecular markers to enhance PEW and PBM in chickens.

Effect of Fluctuation in Al Incorporation on the Microstructure, Bond Lengths, and Surface Properties of an AlxGa1-xN Epitaxial Layer

Xiong Zhang,Shuchang Wang,Hongquan Yang,Yiping Cui 대한금속·재료학회 2015 ELECTRONIC MATERIALS LETTERS Vol.11 No.4

AlxGa1-xN (0.15 ≤ x ≤ 0.87) epilayers were grown on sapphire substrates by metal-organic chemical vapor deposition (MOCVD). The lattice deformation, chemical bonding states, and local electronic structure were characterized by a series of techniques, including X-ray diffraction (XRD), Raman scattering, X-ray photoelectron (XPS), and extended Xray absorption fine structure (EXAFS) spectroscopy. The XRD and Raman spectra revealed that substitution of Ga with Al in AlxGa1-xN epilayers induces a significant contraction in the crystal lattice and a slight expansion of internal parameter u. The XPS measurements indicated the absence of remarkable disorder of the chemical bonds in AlGaN with a high Al content. The EXAFS analysis also demonstrated that the Ga-N and Ga-Al bond lengths are independent of the Al content, whereas the Ga-Ga bond length varies with Al content.

Displacement Interval Prediction Method for Step-like Landslides Considering Deformation State Dynamic Switching

( Linwei Li ),( Yiping Wu ),( Fasheng Miao ),( Yang Xue ),( Longfei Zhang ),( Kang Liao ),( Weifu Teng ),( Honglian Shi ) 대한지질공학회 2019 대한지질공학회 학술발표회논문집 Vol.2019 No.2

To overcome the drawbacks of previous displacement prediction models for step-like landslides, such as poor performance in predicting mutational displacement and unclear reliability of prediction results, this paper proposes a new hybrid method of landslide displacement prediction intervals. Firstly, the combination of SOM network and K-means clustering is implemented to divide the deformation states of step-like landslides into steady state and mutational state. Secondly, on the basis of expanding the mutational state samples through the comprehensive application of the engineering geology analogy method and the adaptive synthetic sampling algorithm, the random forest algorithm is used to establish an ensemble classifier for recognizing the landslide deformation states automatically. Finally, based on the Bootstrap-KELM-BPNN model, an interval prediction framework considering the dynamic switching of landslide deformation states is constructed to realize the dynamic prediction of landslide displacement. Taking Baishuihe landslide, a typical step-like landslide in the Three Gorges Reservoir Area, as an example, the dataset of XD01 monitoring point from June 2006 to December 2016 are explored to verify the effectiveness, accuracy and reliability of the proposed method.

Serotype-Independent Protection against Pneumococcal Infections Elicited by Intranasal Immunization with Ethanol-Killed Pneumococcal Strain, SPY1

XiuYu Xu,Qun Zhang,Jiangping Meng,Yiping Wang,Jie Zheng,Kaifeng Wu,Xuemei Zhang,Yibing Yin 한국미생물학회 2014 The journal of microbiology Vol.52 No.4

The 23-valent polysaccharide vaccine and the 7-valent pneumococcalconjugate vaccine are licensed vaccines that protectagainst pneumococcal infections worldwide. However,the incidence of pneumococcal diseases remains high in lowincomecountries. Whole-cell vaccines with high safety andstrong immunogenicity may be a favorable choice. We previouslyobtained a capsule-deficient Streptococcus pneumoniaemutant named SPY1 derived from strain D39. As anattenuated live pneumococcal vaccine, intranasal immunizationwith SPY1 elicits broad serotype-independent protectionagainst pneumococcal infection. In this study, forsafety consideration, we inactivated SPY1 with 70% ethanoland intranasally immunized BALB/c mice with killed SPY1plus cholera toxin adjuvant for four times. Results showedthat intranasal immunization with inactivated SPY1 inducedstrong humoral and cellular immune responses. Intranasalimmunization with inactivated SPY1 plus cholera toxin adjuvantelicited effective serotype-independent protection againstthe colonization of pneumococcal strains 19F and 4 as well aslethal infection of pneumococcal serotypes 2, 3, 14, and 6B. The protection rates provided by inactivated SPY1 againstlethal pneumococcal infection were comparable to those ofcurrently used polysaccharide vaccines. In addition, vaccinespecificB-cell and T-cell immune responses mediated theprotection elicited by SPY1. In conclusion, the 70% ethanolinactivatedpneumococcal whole-cell vaccine SPY1 is a potentiallysafe and less complex vaccine strategy that offersbroad protection against S. pneumoniae.