Relationship between 18F-FDG PET/CT Semi-Quantitative Parameters and International Association for the Study of Lung Cancer, American Thoracic Society/European Respiratory Society Classification in Lung Adenocarcinomas

Bu Lihong,Tu Ning,Wang Ke,Zhou Ying,Xie Xinli,Han Xingmin,Lin Huiqin,Feng Hongyan 대한영상의학회 2022 Korean Journal of Radiology Vol.23 No.1

Objective: To investigate the relationship between 18F-FDG PET/CT semi-quantitative parameters and the International Association for the Study of Lung Cancer, American Thoracic Society/European Respiratory Society (IASLC/ATS/ERS) histopathologic classification, including histological subtypes, proliferation activity, and somatic mutations. Materials and Methods: This retrospective study included 419 patients (150 males, 269 females; median age, 59.0 years; age range, 23.0–84.0 years) who had undergone surgical removal of stage IA–IIIA lung adenocarcinoma and had preoperative PET/CT data of lung tumors. The maximum standardized uptake values (SUVmax), background-subtracted volume (BSV), and background-subtracted lesion activity (BSL) derived from PET/CT were measured. The IASLC/ATS/ERS subtypes, Ki67 score, and epidermal growth factor/anaplastic lymphoma kinase (EGFR/ALK) mutation status were evaluated. The PET/CT semiquantitative parameters were compared between the tumor subtypes using the Mann–Whitney U test or the Kruskal–Wallis test. The optimum cutoff values of the PET/CT semi-quantitative parameters for distinguishing the IASLC/ATS/ERS subtypes were calculated using receiver operating characteristic curve analysis. The correlation between the PET/CT semi-quantitative parameters and pathological parameters was analyzed using Spearman’s correlation. Statistical significance was set at p < 0.05. Results: SUVmax, BSV, and BSL values were significantly higher in invasive adenocarcinoma (IA) than in minimally IA (MIA), and the values were higher in MIA than in adenocarcinoma in situ (AIS) (all p < 0.05). Remarkably, an SUVmax of 0.90 and a BSL of 3.62 were shown to be the optimal cutoff values for differentiating MIA from AIS, manifesting as pure ground-glass nodules with 100% sensitivity and specificity. Metabolic-volumetric parameters (BSV and BSL) were better potential independent factors than metabolic parameters (SUVmax) in differentiating growth patterns. SUVmax and BSL, rather than BSV, were strongly or moderately correlated with Ki67 in most subtypes, except for the micropapillary and solid predominant groups. PET/CT parameters were not correlated with EGFR/ALK mutation status. Conclusion: As noninvasive surrogates, preoperative PET/CT semi-quantitative parameters could imply IASLC/ATS/ERS subtypes and Ki67 index and thus may contribute to improved management of precise surgery and postoperative adjuvant therapy.

Double Tract Reconstruction Reduces Reflux Esophagitis and Improves Quality of Life after Radical Proximal Gastrectomy for Patients with Upper Gastric or Esophagogastric Adenocarcinoma

Xin Ji,Chenggen Jin,Ke Ji,Ji Zhang,Xiaojiang Wu,Ziyu Jia,Zhaode Bu,Jiafu Ji 대한암학회 2021 Cancer Research and Treatment Vol.53 No.3

Purpose The aim of the present study was to compare the difference between double tract reconstruction and esophagogastrostomy.Materials and Methods Patients who underwent radical proximal gastrectomy with esophagogastrostomy or double tract reconstruction were included in this study.Results Sixty-four patients were included in this study and divided into two groups according to reconstruction method. The two groups were well balanced in perioperative safety and 3-year overall survival (OS). The rates of postoperative reflux esophagitis in the double tract reconstruction group and esophagogastrostomy group were 8.0% and 30.8%, respectively (p=0.032). Patients in the double tract reconstruction group had a better global health status (p < 0.001) and emotional functioning (p < 0.001), and complained less about nausea and vomiting (p < 0.001), pain (p=0.039), insomnia (p=0.003), and appetite loss (p < 0.001) based on the European Organization for Research and Treatment of Cancer (EORTC) QLQ-C30 questionnaire. Regarding the EORTC QLQ-STO22 questionnaire, patients in the double tract reconstruction group complained less about dysphagia (p=0.030), pain (p=0.008), reflux (p < 0.001), eating (p < 0.001), anxiety (p < 0.001), dry mouth (p=0.007), and taste (p=0.001). The multiple linear regression analysis showed that reconstruction method, postoperative complications, reflux esophagitis, and operation duration had a linear relationship with the global health status score.Conclusion Double tract reconstruction could better prevent reflux esophagitis and improve quality of life without scarifying perioperative safety or 3-year OS.

Torque-Angle-Based Direct Torque Control for Interior Permanent-Magnet Synchronous Motor Drivers in Electric Vehicles

Xin Qiu,Wenxin Huang,Feifei Bu 전력전자학회 2013 JOURNAL OF POWER ELECTRONICS Vol.13 No.6

A modified direct torque control (DTC) method based on torque angle is proposed for interior permanent-magnet synchronous motor (IPMSM) drivers used in electric vehicles (EVs). Given the close relationship between torque and torque angle, proper voltage vectors are selected by the proposed DTC method to change the torque angle rapidly and regulate the torque quickly. The amplitude and angle of the voltage vectors are determined by the torque loop and stator flux-linkage loop, respectively, with the help of the position of the stator flux linkage. Furthermore, to satisfy the torque performance request of EVs, the nonlinear dead-time of the invertor caused by parasitic capacitances is considered and compensated to improve steady torque performance. The stable operation region of the IPMSM DTC driver for voltage and current limits is investigated for reliability. The experimental results prove that the proposed DTC has good torque performance with a brief control structure.

Torque-Angle-Based Direct Torque Control for Interior Permanent-Magnet Synchronous Motor Drivers in Electric Vehicles

Qiu, Xin,Huang, Wenxin,Bu, Feifei The Korean Institute of Power Electronics 2013 JOURNAL OF POWER ELECTRONICS Vol.13 No.6

A modified direct torque control (DTC) method based on torque angle is proposed for interior permanent-magnet synchronous motor (IPMSM) drivers used in electric vehicles (EVs). Given the close relationship between torque and torque angle, proper voltage vectors are selected by the proposed DTC method to change the torque angle rapidly and regulate the torque quickly. The amplitude and angle of the voltage vectors are determined by the torque loop and stator flux-linkage loop, respectively, with the help of the position of the stator flux linkage. Furthermore, to satisfy the torque performance request of EVs, the nonlinear dead-time of the invertor caused by parasitic capacitances is considered and compensated to improve steady torque performance. The stable operation region of the IPMSM DTC driver for voltage and current limits is investigated for reliability. The experimental results prove that the proposed DTC has good torque performance with a brief control structure.

Stable Isotopes Reveal Water Vapor Sources of Precipitation over the Jiaolai Plain, Shandong Peninsula, China

Ying Wang,Bu-li Cui,Dong-sheng Li,Ya-xuan Wang,Wan-xin Yu,He-hua Zong 한국기상학회 2022 Asia-Pacific Journal of Atmospheric Sciences Vol.58 No.2

A prerequisite for using isotopic techniques to study the regional water cycle of a mountainous area is to examine the stable isotopic composition of precipitation. These findings are of great significance for an in-depth understanding of water cycle processes. In this study, each precipitation event was sampled and used to investigate the characteristics of stable hydrogen and oxygen isotopes in precipitation over the Jiaolai Plain and its surrounding areas. NCEP/NCAR data was used for the wind speed and direction, relative humidity, and precipitable amount in the study area during the sampling period. The water vapor sources of the precipitation over the plain were revealed through a comparative analysis of seasonal variations in precipitation isotopes between Global Network of Isotopes in Precipitation stations located along different vapor transport paths. The results showed that the local meteoric water line was δ2H = 6.38 δ18O + 0.72, with a gradient of less than 8. This indicates that the precipitation process was affected by non-equilibrium evaporation occurring when the drops fell below the cloud base. Temperature and amount effects were observed in the δ18O of the precipitation, although the altitude effect was not significant. The water vapor source of the precipitation was predominantly controlled by the East Asian Monsoon from June to September, with the primary source being evaporation from the adjacent Pacific Ocean. The plain was controlled by the Westerlies from October through May, with the predominant vapor source being local evaporation. Water vapor from the polar region had a minimal impact. These findings can serve as the basis for studying surface water–groundwater–seawater transformations.

Research on Dynamic Decoupling Control Method of Three-phase Bearingless Induction Motor

Wen-shao Bu,Chun-xiao Lu,Cong-lin Zu,Xin-wen Niu 보안공학연구지원센터 2014 International Journal of Control and Automation Vol.7 No.5

Robust Sliding-mode Observer-based Sensor Fault Estimation, Actuator Fault Detection and Isolation for Uncertain Nonlinear Systems

Junqi Yang,Fanglai Zhu,Xin Wang,Xuhui Bu 제어·로봇·시스템학회 2015 International Journal of Control, Automation, and Vol.13 No.5

This paper deals with the issues of sensor fault estimation, actuator fault detection and isolation for a class of uncertain nonlinear systems. By taking the sensor fault vector as a part of an extended state vector, the original system with sensor faults, actuator faults and unknown inputs is transformed into an augmented singular system which is just with actuator faults and unknown inputs. For the constructed singular system, a robust sliding-mode observer is developed to simultaneously estimate the states and sensor faults of original system, and the observer gain matrices are computed in terms of linear matrix inequalities by solving an optimization problem. Then an actuator fault detector is designed to detect actuator faults when ones occur, and multiple observers used as actuator fault isolators are proposed to identify which actuator is with fault. Finally, a simulation example is given to illustrate the effectiveness of the proposed methods.

An Improved Method for Rapid Preparation of Oligodendrocytespecific Rabbit Polyclonal Antibody

Tao Wang,Wei Wang,Lintao Jia,Xin Bu,Fang Wang,Libo Yao,Bin Zhang 한국생물공학회 2011 Biotechnology and Bioprocess Engineering Vol.16 No.3

Antibodies are important tools in the study of protein function and diagnostic tests. However, traditional antiserum preparation requires a time-consuming immunization protocol and subsequent purification of polyclonal antibodies. In this study, a rapid and efficient method for polyclonal antibody preparation has been developed. Juxtanodin (JN) and silent information regulator-2 (Sirt2),both of which are oligodendrocyte-specific proteins, were used for antibody preparation. The N-terminal 170 amino acids of JN (JN170) and amino acids 231-351 of Sirt2 (Sirt2-121) were expressed as GST-tagged proteins from a pET-41a(+) vector in E. coli strain BL21 (DE3) cells. The fusion proteins were purified and used to immunize rabbits following both a traditional protocol, in which antigen was presented biweekly, and a modified rapid protocol, in which the immunization on day 1 was boosted on days 5and 28. ELISA, Western blot analysis and immunofluorescent staining showed that antibodies produced via the rapid protocol could recognize these two oligodendrocytespecific proteins in vitro and in the rat central nervous system (CNS), respectively, similar to those produced with the traditional protocol. Thus, our study provides a novel rapid method to prepare high specificity antibodies via a modified immunization protocol and subsequent antibody purification.

Comparative Proteomic Analysis of Changes in the Bovine Whey Proteome during the Transition from Colostrum to Milk

Zhang, Le-Ying,Wang, Jia-Qi,Yang, Yong-Xin,Bu, Deng-Pan,Li, Shan-Shan,Zhou, Ling-Yun Asian Australasian Association of Animal Productio 2011 Animal Bioscience Vol.24 No.2

Bovine whey protein expression patterns of colostrum are much different from that of milk. Moreover, bovine colostrum is an important source of protective, nutritional and developmental factors for the newborn. However, to our knowledge, no research has been performed to date using a comparative proteomic method on the changes in the bovine whey proteome during the transition from colostrum to milk. This study therefore separated whey protein of days 1, 3, 7 and 21 after calving using two dimension electrophoresis. Differentially expressed proteins at different collection times were identified using high-performance liquid chromatography in tandem with mass spectrometry (LC/MS) and validated by enzyme-linked immunosorbent assay (ELISA) in order to understand the developmental changes in the bovine whey proteome during the transition from colostrum to milk. The expression patterns of whey protein of days 1 and 3 post-partum were similar except that immunoglobulin G was down-regulated on day 3, and four proteins were found to be down-regulated on days 7 and 21 compared with day 1 after delivering, including immunoglobulin G, immunoglobulin M, albumin, and lactotransferrin, which are involved in immunity and molecule transport. The results of this study confirm the comparative proteomic method has the advantage over other methods such as ELISA and immunoassays in that it can simultaneously detect more differentially expressed proteins. In addition, the difference in composition of milk indicates a need for adjustment of the colostrum feeding regimen to ensure a protective immunological status for newborn calves.

XPC 939A>C and 499C>T Polymorphisms and Skin Cancer Risk: a Meta-analysis

Ji, Geng,Lin, Yuan,Cao, Song-Yu,Li, Luo-Zhu,Chen, Xin-Long,Sun, Bu-Mei,Chen, Chuan-Jun,Ma, Hong-Xia Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.10

The xeroderma pigmentosum complementation group C gene (XPC) has been identified as important for repairing UV-related DNA damage. Some subtle changes in this gene may impair repair efficiency and influence susceptibility to human cancers, including skin cancer. Two polymorphisms in XPC, 939A>C (rs2228001) and 499C>T (rs2228000), are considered to have possible associations with the risk of skin cancer, but the reported results have been inconsistent. Here we performed a meta-analysis of the available evidence regarding the relationship between these two polymorphisms and the risk of skin cancer. All relevant studies were searched using PubMed, Embase and Web of Science before February 2012. A total of 8 case-control studies were included in this analysis, and no convincing associations between the two polymorphisms and risk of skin cancer were observed in any of the genetic models. Stratified analyses by skin cancer type also did not detect significant associations in any subgroup. This meta-analysis suggested that the XPC 939A>C and 499C>T polymorphisms may have little involvement in susceptibility to skin cancer.