The Aurora Kinase Inhibitor CYC116 Promotes the Maturation of Cardiomyocytes Derived from Human Pluripotent Stem Cells

Xin Xie,Wanzhi Tu,Chenwen Huang,Ziyang Chen,Xinyue Ren,Bingqing He,Xiaoyan Ding,Yuelei Chen,Xin Xie 한국분자세포생물학회 2022 Molecules and cells Vol.45 No.12

Human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) have great potential in applications such as regenerative medicine, cardiac disease modeling, and in vitro drug evaluation. However, hPSC-CMs are immature, which limits their applications. During development, the maturation of CMs is accompanied by a decline in their proliferative capacity. This phenomenon suggests that regulating the cell cycle may facilitate the maturation of hPSC-CMs. Aurora kinases are essential kinases that regulate the cell cycle, the role of which is not well studied in hPSC-CM maturation. Here, we demonstrate that CYC116, an inhibitor of Aurora kinases, significantly promotes the maturation of CMs derived from both human embryonic stem cells (H1 and H9) and iPSCs (induced PSCs) (UC013), resulting in increased expression of genes related to cardiomyocyte function, better organization of the sarcomere, increased sarcomere length, increased number of mitochondria, and enhanced physiological function of the cells. In addition, a number of other Aurora kinase inhibitors have also been found to promote the maturation of hPSC-CMs. Our data suggest that blocking aurora kinase activity and regulating cell cycle progression may promote the maturation of hPSC-CMs.

The Aurora Kinase Inhibitor CYC116 Promotes the Maturation of Cardiomyocytes Derived from Human Pluripotent Stem Cells

Xin Xie,Wanzhi Tu,Chenwen Huang,Ziyang Chen,Xinyue Ren,Bingqing He,Xiaoyan Ding,Yuelei Chen,Xin Xie 한국분자세포생물학회 2022 Molecules and cells Vol.45 No.12

Human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) have great potential in applications such as regenerative medicine, cardiac disease modeling, and in vitro drug evaluation. However, hPSC-CMs are immature, which limits their applications. During development, the maturation of CMs is accompanied by a decline in their proliferative capacity. This phenomenon suggests that regulating the cell cycle may facilitate the maturation of hPSC-CMs. Aurora kinases are essential kinases that regulate the cell cycle, the role of which is not well studied in hPSC-CM maturation. Here, we demonstrate that CYC116, an inhibitor of Aurora kinases, significantly promotes the maturation of CMs derived from both human embryonic stem cells (H1 and H9) and iPSCs (induced PSCs) (UC013), resulting in increased expression of genes related to cardiomyocyte function, better organization of the sarcomere, increased sarcomere length, increased number of mitochondria, and enhanced physiological function of the cells. In addition, a number of other Aurora kinase inhibitors have also been found to promote the maturation of hPSC-CMs. Our data suggest that blocking aurora kinase activity and regulating cell cycle progression may promote the maturation of hPSC-CMs.

Biocatalysis and Fermentation Technology : Thermostable Sites and Catalytic Characterization of Xylanase XYNB of Aspergillus niger SCTCC 400264

( Xin Ran Li ),( Hui Xu ),( Jie Xie ),( Qiao Fu Yi ),( Wei Li ),( Dai Rong Qiao ),( Yi Cao ),( Yu Cao ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.4

In order to improve the expression of heat-resistant xylanase XYNB from Aspergillus niger SCTCC 400264, XynB has been cloned into Pichia pastoris secretary vector pPIC9K. The XynB production of recombinant P. pastoris was four times that of E. coli, and the Vmax and specific activity of XynB reached 2,547.7 umol/mg and 4,757 U/mg, respectively. XynB still had 74% residual enzyme activity after 30 min of heat treatment at 80°C. From the van der Waals force analysis of XYNB (ACN89393 and AAS67299), there is one more oxygen radical in AAS67299 in their catalytic site, indicating that the local cavity is much more free, and it is more optimal for substrate binding, affinity reaction, and proton transfer, etc, and e ventually i ncreasing enzyme activity. The H-bonds analysis of XYNB indicated that there are two more H-bonds in the 33rd Ser of XYNB (AAS67299) than in the 33rd Ala(ACN89393 ), and two H-bonds between Ser70 and Asp67.

The Realization and Simulation of the Wireless Ad-hoc Network

Xin Xie,Yixin Yan 보안공학연구지원센터 2014 International Journal of Future Generation Communi Vol.7 No.5

Anti-tumor Effects and Apoptosis Induction by Realgar Bioleaching Solution in Sarcoma-180 Cells in Vitro and Transplanted Tumors in Mice in Vivo

Xie, Qin-Jian,Cao, Xin-Li,Bai, Lu,Wu, Zheng-Rong,Ma, Ying-Ping,Li, Hong-Yu Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.6

Background: Realgar which contains arsenic components has been used in traditional Chinese medicine (TCM) as an anticancer drug. However, neither Realgar nor its formula are soluble in water. As a result, high dose of Realgar has to be administered to achieve an effective blood medicine concentration, and this is associated with adverse side effects. The objective of the present study was to increase the solubility of a formula using hydrometallurgy technology as well as investigating its effects on in vitro and in vivo cell proliferation and apoptosis in Sarcoma-180 cell line. Materials and Methods: Antiproliferative activity of Realgar Bioleaching Solution (RBS) was evaluated by MTT assay. Further, effects of RBS on cell proliferation and apoptosis were studied using flow cytometry and transmission electron microscopy. Kunming mice were administered RBS in vivo, where arsenic specifically targeted solid tumors. Results: The results indicated that RBS extract potently inhibited the tumor growth of Sarcoma-180 cell line in a dose-dependent manner. Flow cytometry and transmission electron microscopy further indicated that RBS significantly induced cell apoptosis through the inhibition of cell cycle pathway in a dose-dependent manner. Further, on RBS administration to mice, arsenic was specifically targeted to solid tumor.s Conclusions: RBS could substitute for traditional Realgar or its formula to work as a potent tool in cancer treatment.

Controlled canard configuration study for a solid rocket motor based unmanned air vehicle

Xie Kan,Liu Yu,Xin Jianren 대한기계학회 2009 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.23 No.12

This paper presents an aerodynamic study for a target unmanned air vehicle with controlled canard, at cruise Mach number between 0.7 and 0.85, using a solid rocket motor as power. The results of semi-empirical method (DATCOM), computational fluid dynamics (CFD) were compared with wind tunnel tests to evaluate perdition accuracy. Here DATCOM shows big discrepancy in pitching-moment coefficient; the CFD estimation of static aerodynamic force and moment agree well with wind tunnel data. An analysis was focused on the longitudinal data for complicated controlled canard-elements interactions and canard vortex behavior. The canard vortex interaction with tail wing of initial configuration concept with a thick canard (NACA0012) and the final improved configuration with a thin canard (NACA0007) were reviewed. It is proved that the stability and control characteristics of whole TUAV are sensitive to the thickness of canard in transonic peed regime because of different canard vortex intensity and effect generated.

Transfer function modeling of structural vibration of complex aerospace structures based on finite element analysis

Xin Feng Wu,Yongjun Lei,Daokui Li,Yan Xie 대한기계학회 2013 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.27 No.5

An approach to establish the transfer function of complex aerospace structures to express their structural vibration behavior through finite element modeling is proposed. The fundamental idea of the approach is to characterize the vibrations of complicated structures through the transfer function model for an advanced control system design, while the parameters of the model are identified from the response data obtained through finite element analysis. The proposed method comprises four steps, namely, finite element modeling and validation, data preparation based on the finite element analysis, parameter identification of the transfer function, and model validation,and is presented from both frequency and time domains. The developed approach is applied to a cantilever beam, a strap-on launch vehicle,and a local part of the aerospace structure to demonstrate the method’s effectiveness and satisfactory performance.

An Electric-Field Coupled Power Transfer System with a Double-sided LC Network

Xie, Shi-Yun,Su, Yu-Gang,Zhou, Wei,Zhao, Yu-Ming,Dai, Xin The Korean Institute of Power Electronics 2018 JOURNAL OF POWER ELECTRONICS Vol.18 No.1

Electric-field coupled power transfer (ECPT) systems employ a high frequency electric field as an energy medium to transfer power wirelessly. Existing ECPT systems have made great progress in terms of increasing the transfer distance. However, the topologies of these systems are complex, and the transfer characteristics are very sensitive to variations in the circuit parameters. This paper proposes an ECPT system with a double-sided LC network, which employs a parallel LC network on the primary side and a series LC network on the secondary side. With the same transfer distance and output power, the proposed system is simpler and less sensitive than existing systems. The expression of the optimal driving voltage for the coupling structure and the characteristics of the LC networks are also analyzed, including the transfer efficiency, parameter sensitivity and total harmonic distortion. Then, a design method for the system parameters is provided according to these characteristics. Simulations and experiments have been carried out to verify the system properties and the design method.

Infection of tomato by Tomato Yellow Leaf Curl Virus alters the foraging behavior and parasitism of the parasitoid Encarsia formosa on Bemisia tabaci

Xin Liu,Yanyan He,Wen Xie,Qingjun Wu,Youjun Zhang,Yong Liu,Shaoli Wang 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.2

Encarsia formosa Gahan is a solitary endoparasitoid that is commercially reared and released for augmentative biological control of whiteflies including Bemisia tabaci (Gennadius). Bemisia tabaci biotypes B and Q are two most invasive species that greatly reduce crop yields in China by feeding on plant sap and by transmitting Tomato Yellow Leaf Curl Virus (TYLCV). The effects of TYLCV infection of tomato on E. formosa foraging on B. tabaci B and Q are unknown. In Y-tube olfactometer assays in the present study, E. formosa significantly preferred TYLCVinfected tomato plants over TYLCV-free plants. The wasp females also significantly preferred TYLCV-infected tomato plants infested with 3rd-instar nymphs of B. tabaci biotype Q over TYLCV-free plants with biotype Q nymphs. However, no significant differences were observed when B. tabaci biotype B was infested on tomato plants. The oviposition bioassays confirmed that TYLCV infection on tomato plants resulted in the recruitment of parasitoids. These results indicate that TYLCV-infection of tomato increase the foraging of E. formosa on B. tabaci, as differs on the B and Q biotypes.

A Cloud-Edge Collaborative Computing Task Scheduling and Resource Allocation Algorithm for Energy Internet Environment

( Xin Song ),( Yue Wang ),( Zhigang Xie ),( Lin Xia ) 한국인터넷정보학회 2021 KSII Transactions on Internet and Information Syst Vol.15 No.6

To solve the problems of heavy computing load and system transmission pressure in energy internet (EI), we establish a three-tier cloud-edge integrated EI network based on a cloud-edge collaborative computing to achieve the tradeoff between energy consumption and the system delay. A joint optimization problem for resource allocation and task offloading in the three-tier cloud-edge integrated EI network is formulated to minimize the total system cost under the constraints of the task scheduling binary variables of each sensor node, the maximum uplink transmit power of each sensor node, the limited computation capability of the sensor node and the maximum computation resource of each edge server, which is a Mixed Integer Non-linear Programming (MINLP) problem. To solve the problem, we propose a joint task offloading and resource allocation algorithm (JTOARA), which is decomposed into three subproblems including the uplink transmission power allocation sub-problem, the computation resource allocation sub-problem, and the offloading scheme selection sub-problem. Then, the power allocation of each sensor node is achieved by bisection search algorithm, which has a fast convergence. While the computation resource allocation is derived by line optimization method and convex optimization theory. Finally, to achieve the optimal task offloading, we propose a cloud-edge collaborative computation offloading schemes based on game theory and prove the existence of Nash Equilibrium. The simulation results demonstrate that our proposed algorithm can improve output performance as comparing with the conventional algorithms, and its performance is close to the that of the enumerative algorithm.