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( Xihong Zhao ),( Jun Wang ),( Fereidoun Forghani ),( Joong Hyun Park ),( Myoung Su Park ),( Kun Ho Seo ),( Deog Hwan Oh ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.12
Conventional molecular detection methods cannot distinguish between viable and dead Escherichia coli O157 cells. In this study, the loop-mediated isothermal amplification (LAMP) method combined with propidium monoazide (PMA) treatment was developed to selectively detect viable E. coli O157 cells. Four primers, including outer primers and inner primers, were specially designed for the recognition of six distinct sequences on the serogroups (O157) of the specific rfbE gene of the E. coli O157 genome. PMA selectively penetrated through the compromised cell membranes and intercalated into DNA. Amplification of DNA from dead cells was completely inhibited by 3.0 μg/ml PMA, whereas the DNA derived from viable cells was amplified remarkably within 1 h by PMA-LAMP. Exhibiting high sensitivity and specificity, PMA-LAMP is a suitable method for evaluating the inactivation efficacy of slightly acidic electrolyzed water in broth. PMA-LAMP can selectively detect viable E. coli O157 cells. This study offers a novel molecular detection method to distinguish between viable and dead E. coli O157 cells.
Xihong Zhao,Li Wang,Jin Chu,Yanmei Li,Yanyan Li,Zhenbo Xu,Lin Li,Mark E. Shirtliff,Xiaowei He,Yao Liu,Jihua Wang,Liansheng Yang 한국식품과학회 2010 Food Science and Biotechnology Vol.19 No.6
A loop-mediated isothermal amplification (LAMP) method for rapid detection of the food-borne Salmonella strains had been developed and evaluated in this study. The optimal reaction condition was found to be 65℃ for 45 min, with the detection limit as 1 pg DNA/tube and 100 CFU/reaction. Application of LAMP assays was performed on 214 food-borne Salmonella strains using a rapid procedure and easy result confirmation, where the specificity of LAMP and polymerase chain reaction (PCR)assays was 97.7% (209/214) and 91.6% (196/214),respectively; with a 100% specificity for both assays.
Xihong Zhao,Li Wang,Jin Chu,Yanyan Li,Yanmei Li,Lin Li,Mark E. Shirtliff,Xiaowei He,Yao Liu,Jihua Wang,Liansheng Yang,Zhenbo Xu 한국식품과학회 2010 Food Science and Biotechnology Vol.19 No.5
A loop-mediated isothermal amplification (LAMP) method for rapid detection of the foodborne Vibrio parahaemolyticus strains and related virulent factors had been developed and evaluated in this study. Six primers, including outer primers, inner primers, and loop primers, were specially designed for recognizing 8 distinct sequences on 3 target genes, which were tlh, tdh, and trh. The detection limits were found to be 100, 100 fg, and 1 pg DNA/tube for tlh, tdh, and trh, respectively. Application of LAMP assays were performed on 368 foodborne V. parahaemolyticus strains, the sensitivities of LAMP assays for the tlh, tdh, and trh were 100, 95.6, and 96.4%, and the negative predictive values (NPV) were 100, 84.7, and 93.1%, respectively; with a 100% specificity and positive predictive value (PPV) for all 3 target genes.
Field Testing and Analysis During Top-down Construction of Super-tall Buildings in Shanghai
Yongjing Tang,Xihong Zhao 대한토목학회 2016 KSCE JOURNAL OF CIVIL ENGINEERING Vol.20 No.2
Although certain measurements regarding super-tall buildings constructed using the top-down method have been published, new advances in top-down construction have been developed with the construction of super-tall buildings in China. This paper presents the theory of soil-structure interactions in pile foundations and extends it to the top-down construction method. Based on this theory, the forces and the deformation of the diaphragm wall, slab and soldier piles at various stages of construction can be computed. Two typical tall buildings of 60 and 37 stories with deeply embedded 4-level and 5-level basements located in Shanghai were used as case studies of the vertical displacements of their diaphragm walls and soldier piles, the deflections of the diaphragm walls, the earth pressures, and the rebar stresses during top-down construction. The values measured in the field agree well with the predicted values from soilstructure interactions theory and statistical-empirical formulas. Two additional super-tall buildings of 101 and 121 stories, in which the 4- level and 5-level basements and the main buildings are round, are discussed regarding their unique deformational characteristics. In addition, the diaphragm wall can serve a load-sharing function. These engineering case studies, including the comprehensive predictions of deformation based on field tests and estimates using statistical formulas, can improve tall building design.
Significance of Viable but Nonculturable Escherichia coli: Induction, Detection, and Control
( Tian Ding ),( Yuanjie Suo ),( Qisen Xiang ),( Xihong Zhao ),( Shiguo Chen ),( Xingqian Ye ),( Donghong Liu ) 한국미생물 · 생명공학회 2017 Journal of microbiology and biotechnology Vol.27 No.3
Diseases caused by foodborne or waterborne pathogens are emerging. Many pathogens can enter into the viable but nonculturable (VBNC) state, which is a survival strategy when exposed to harsh environmental stresses. Pathogens in the VBNC state have the ability to evade conventional microbiological detection methods, posing a significant and potential health risk. Therefore, controlling VBNC bacteria in food processing and the environment is of great importance. As the typical one of the gram-negatives, Escherichia coli (E. coli) is a widespread foodborne and waterborne pathogenic bacterium and is able to enter into a VBNC state in extreme conditions (similar to the other gram-negative bacteria), including inducing factors and resuscitation stimulus. VBNC E. coli has the ability to recover both culturability and pathogenicity, which may bring potential health risk. This review describes the concrete factors (nonthermal treatment, chemical agents, and environmental factors) that induce E. coli into the VBNC state, the condition or stimulus required for resuscitation of VBNC E. coli, and the methods for detecting VBNC E. coli. Furthermore, the mechanism of genes and proteins involved in the VBNC E. coli is also discussed in this review.