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Xihong Zhao,Li Wang,Jin Chu,Yanyan Li,Yanmei Li,Lin Li,Mark E. Shirtliff,Xiaowei He,Yao Liu,Jihua Wang,Liansheng Yang,Zhenbo Xu 한국식품과학회 2010 Food Science and Biotechnology Vol.19 No.5
A loop-mediated isothermal amplification (LAMP) method for rapid detection of the foodborne Vibrio parahaemolyticus strains and related virulent factors had been developed and evaluated in this study. Six primers, including outer primers, inner primers, and loop primers, were specially designed for recognizing 8 distinct sequences on 3 target genes, which were tlh, tdh, and trh. The detection limits were found to be 100, 100 fg, and 1 pg DNA/tube for tlh, tdh, and trh, respectively. Application of LAMP assays were performed on 368 foodborne V. parahaemolyticus strains, the sensitivities of LAMP assays for the tlh, tdh, and trh were 100, 95.6, and 96.4%, and the negative predictive values (NPV) were 100, 84.7, and 93.1%, respectively; with a 100% specificity and positive predictive value (PPV) for all 3 target genes.
Xihong Zhao,Li Wang,Jin Chu,Yanmei Li,Yanyan Li,Zhenbo Xu,Lin Li,Mark E. Shirtliff,Xiaowei He,Yao Liu,Jihua Wang,Liansheng Yang 한국식품과학회 2010 Food Science and Biotechnology Vol.19 No.6
A loop-mediated isothermal amplification (LAMP) method for rapid detection of the food-borne Salmonella strains had been developed and evaluated in this study. The optimal reaction condition was found to be 65℃ for 45 min, with the detection limit as 1 pg DNA/tube and 100 CFU/reaction. Application of LAMP assays was performed on 214 food-borne Salmonella strains using a rapid procedure and easy result confirmation, where the specificity of LAMP and polymerase chain reaction (PCR)assays was 97.7% (209/214) and 91.6% (196/214),respectively; with a 100% specificity for both assays.