Effects of Lactobacillus salivarius Ren on cancer prevention and intestinal microbiota in 1, 2-dimethylhydrazine-induced rat model

Ming Zhang,Xing Fan,Bing Fang,Chengzhen Zhu,Jun Zhu,Fazheng Ren 한국미생물학회 2015 The journal of microbiology Vol.53 No.6

Probiotics have been suggested as a prophylactic measure in colon cancer. The aim of this study was to investigate the impact of Lactobacillus salivarius Ren (Ren) in modulating colonic microbiota structure and colon cancer incidence in a rat model after injection with 1,2-dimethyl hydrazine (DMH). The results indicated that oral administration of Ren could effectively suppress DMH-induced colonic carcinogenesis. A significant decrease in cancer incidence (87.5% to 25%) was detected in rats fed with a dose of 5 × 1010 CFU/kg bodyweight per day. Using denaturing gradient gel electrophoresis and Real-time PCR combined with multivariate statistical methods, we demonstrated that injection with DMH significantly altered the rat gut microbiota, while Ren counteracted these DMH-induced adverse effects and promoted reversion of the gut microbiota close to the healthy state. Tvalue biplots followed by band sequencing identified 21 bacterial strains as critical variables affected by DMH and Ren. Injection of DMH significantly increased the amount of Ruminococcus species (sp.) and Clostridiales bacteria, as well as decreasing the Prevotella sp. Administration of Ren reduced the amount of Ruminococcus sp., Clostridiales bacteria, and Bacteroides dorei, and increased the amount of Prevotella. Real-time PCR results were consistent with the results derived by t-value biplots. These findings suggested that Ren is a potential agent for colon cancer prevention. In conclusion, the results in the present study suggest a potential therapeutic approach based on the modulation of intestinal microflora by probiotics may be beneficial in the prevention of colorectal carcinogenesis.

A conventional route to scalable morphology-controlled regular structures and their superhydrophobic/hydrophilic properties for biochips application

Ren, Hong-Xuan,Chen, Xing,Huang, Xing-Jiu,Im, Maesoon,Kim, Dong-Haan,Lee, Joo-Hyung,Yoon, Jun-Bo,Gu, Ning,Liu, Jin-Huai,Choi, Yang-Kyu Royal Society of Chemistry 2009 Lab on a chip Vol.9 No.15

<P>We use a conventional and straightforward route to fabricate scalable morphology-controlled regular structures. This route is based on the etching of PDMS microlens array in CF<SUB>4</SUB> and CF<SUB>4</SUB>/O<SUB>2</SUB> plasma. PDMS microlens array can be changed to regularly isolated microdot structures array in CF<SUB>4</SUB> plasma. Microbowl shaped structures array can be reached in CF<SUB>4</SUB>/O<SUB>2</SUB> plasma. Moreover, a set of structures after CF<SUB>4</SUB> plasma treatment display superhydrophobicity, while a set of structures after CF<SUB>4</SUB>/O<SUB>2</SUB> plasma treatment present hydrophilicity. DNA molecules can be readily enriched on the hydrophilic surface. We believe that the regular structure array surfaces provide a useful inspiration towards biomolecular detection and transportation in biochips.</P> <P>Graphic Abstract</P><P>Morphology-controlled regular structures and their opposite wettabilities can be obtained based on the etching of PDMS microlens array in CF<SUB>4</SUB> and CF<SUB>4</SUB>/O<SUB>2</SUB> plasma. DNA molecules enrichment is also investigated. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=b905804d'> </P>

A NOVEL ANTI-ESTROGEN RECEPTOR TARGETED WATER-SOLUBLE QDs FOR BREAST CANCER CELL IMMUNOFLUORESCENT LABELING

XING REN,Jing-Yuan Wang,HAILONG HUANG,ZHELI XU,GUANG SUN 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2014 NANO Vol.9 No.7

This research is aimed to develop a novel kind of biomarker based on water-soluble quantum dots(QDs) conjugated with estrogen receptor (ER) for the detection of breast cancer (BC). To achievethe purpose, hydrophobic octadecylamine-coated QDs were encapsulated with amphiphilic bio-compatible centipede-like polymer p(aspartate)-Na-graft-p(ethylene glycol)-dodecylamine(PASP-Na-g-PEG-DDA), and then modi¯ed with ER monoclonal antibodies, which is one ofthe most popular biomarkers for the detection and management of breast cancer worldwide. Thetargeted QDs kept the original optical property of QDs. The cytotoxicity in vitro experimentsshowed that QDs-ER probes had no obvious toxic side e®ects. Remarkably, confocal laser scanningmicroscopy (CLSM) revealed that the localized °uorescence appeared around the nucleus of humanbreast cancer cell MCF7 (ER positive) compared with that of human breast cancer cellMBA-MD-231 (ER negative). According to the above analysis, this new kind of probe has greatpotential in the diagnosis of breast tumor in vitro in future clinical application.

중국 화장품시장에서 외국계 공급업체와 중국 현지 소매업체간 관계의 질에 영향을 미치는 요인에 관한 연구

임성요(Xing Yao Ren),오세조(Sejo Oh),성민(Min Sung) 한국유통학회 2006 流通硏究 Vol.11 No.4

이 연구는 중국 화장품 시장에서 외국계 공급업체와 현지 소매업체간 관계의 질 변수인 신뢰와 결속에 영향을 미치는 선행변수들을 규명하는데 초점을 맞추었다. 연구대상은 중국 상하이 지역에서 외국계 화장품 공급업체와 거래하고 있는 현지의 뷰티숍으로 선정하였고 설문조사를 수행하여 실증분석을 하였다. 표본은 상하이의 전체 뷰티숍 리스트를 바탕으로 중ㆍ대형 뷰티숍을 단순 무작위 표본추출법에 의해 선발하였고, 그 수는 72개였다. 회귀 분석을 통해 가설을 검증한 결과, 중국 현지 소매업체의 신뢰를 증가시키는 외국계 공급업체의 능력, 문화적 민감성, 그리고 현지 소매업체와의 관시의 영향력과 현지 소매업체의 결속을 증가시키는 외국계 공급업체의 업무 공식화와 문화적 민감성의 영향력 모두가 존재하는 것으로 나타났다. 또한, 공급업체에 대한 소매업체의 결속을 증가시키는 소매업체의 신뢰의 영향력이 존재하는 것으로 나타났다. 그러나 신뢰를 증가시키는 업무 공식화와 결속을 증가시키는 관시의 직접적인 영향력은 나타나지 않았다. 끝으로, 이 연구의 이론적 의의와 관리적 시사점들이 논의되었고, 연구의 한계점들과 향후의 연구방향들이 제시되었다. This study focuses on finding out the determinants of relational quality between the supplier and the retailer in the Chinese cosmetics market. A total of 72 questionnaires were collected by surveying beauty shops which mainly do business with the foreign cosmetics suppliers in Shanghai area. The findings support the premise that a retailer’s trust in a supplier is positively related to the retailer’s commitment. Supplier’s ability, guanxi and cultural sensitivity have positive impacts on trust. Formalization and cultural sensitivity affect commitment positively. But the formalization is not significantly related to the retailer’s trust. The guanxi is not found to be significantly related to the retailer’s commitment yet. Finally, the authors discuss some theoretical contributions, managerial implications. And then, they present limitations of this study and the future research directions.

A Comparable Study on the Microwave Absorption Properties of Al/Fe/Co Doped OMC/Paraffin Wax Composites

Yanyan Ren,Hongfeng Li,Guanglei Wu,Le Yang,Chenhui Zheng,LIUDING WANG,Fang Ren,Hui Xing,HONGJING WU 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2016 NANO Vol.11 No.2

Al/Fe/Co doped ordered mesoporous carbon (OMC) composites have been synthesized by a facile method, and the influence of dopant on the electromagnetic (EM) and microwave absorption properties was investigated in the frequency range of 2–18 GHz. Compared with Fe/Co– OMC composites, the Al–OMC nanocomposite played a great role in adjusting values and frequency dependence of complex permittivity, which gives rise to significant improved microwave absorption and reduced thickness of the corresponding paraffin wax composites. Reflection loss (RL) values less than -5 dB and -10 dB were obtained in the frequency range of 9.2–18 GHz and 10.7–14.7 GHz with a single thickness of 2.00 mm, respectively. Such enhanced EM wave absorption property of the Al–OMC/paraffin wax composite was ascribed to its superior impedance matching characteristic. Thin thickness, broadband absorption microwave absorbers with Al doped OMC nanocomposites were obtained.

Spatial Analysis of Rural Medical Facilities Using Huff Model : A Case Study of Lankao County, Henan Province

Pengyan Zhang,,Xing Ren,Qianqian Zhang,Jianjian He,Yongmin Chen 보안공학연구지원센터 2015 International Journal of Smart Home Vol.9 No.1

Rural health care reformed in the new situation in China, indicating that the construction of medical facilities not only to solve difficult medical problems, but also to take into account the efficiency and fairness of the use of medical resources allocation. Facing these problems, the paper aims to assess the spatial distribution of rural medical services by using geographic information systems (GIS) and spatial accessibility indexes. Lankao County in Henan Province and 21 hospitals of township and county are selected as study samples. First adopted ArcGIS10.0 to collate data. Second, the authors established a network dataset to analysis the maximum coverage for hospital services space accessibility. At last, established Huff model and built a scientific evaluation for the current hospitals’ distribution. There are several findings from the case study. First, the distribution of medical facility is unbalanced in rural areas in term of per capita, such as opportunity for assessing medical services and the convenience of utilizing medical services. Second, by using the Huff Model to calculate the potential population in service regions, the Huff Model to calculate the potential population in service regions, the results showed the low efficiency of the use of medical resources and the problems in these regions.

An Improved LDV-Hop Localization Algorithm in Wireless Sensor Networks

Xiaoan Bao,Zhongtao Ren,Na Zhang,Xing Hu,Tongshu Wu,Yangqiu Liu 보안공학연구지원센터 2014 International Journal of Control and Automation Vol.7 No.10

Localization of sensor nodes is a fundamental and a key technology in wireless sensor networks, it has been widely studied by many universities and research institutions in recent years and many localization algorithms have been put forward by scholars from diverse aspects. A new localization algorithm, which is based on the LDV-Hop algorithm, is proposed in this paper. Firstly, to reduce the localization error we modify the average one-hop distance between anchor nodes, and the average one-hop distance used for each unknown node's location is also modified through weighting the received average one-hop distance from anchor nodes. Then, to solve the problem of low localization coverage which is the main disadvantage of LDV-Hop localization algorithm, we regard regular node with newly estimated location as anchor node and utilize believable factor threshold to restrain the error accumulation brought by this method.

Characterization of Ecdysteroid UDP-Glucosyltransferase Gene Promoter from Bombyx mori Nucleopolyhedrovirus

Xing-Jia Shen,Yong-Zhu Yi,Shun-Ming Tang,Zhi-Fang Zhang,Yi-Ren Li,Jia-Lu He,Xiang-Fu Wu 한국잠사학회 2004 International Journal of Industrial Entomology Vol.8 No.2

Bombyx mori nucleopolyhedrovirus (BmNPV) ecdysteroid UDP-glucosyltransferase gene (egt) promoter fragments of different lengths were amplified from BmNPV ZJ-8 genomic DNA by PCR. Reporter plasmids pBmegt542-luc, pBmegt309-luc and pBmegt159- luc with luciferase (luc) driven by egt promoters were constructed. Both in vitro and in vivo expressions showed that BmNPV egt promoter activity requires the transactivation of viral factor(s), and expression of luc was detected earliest at 24 hrs post infection (pi). BmNPV ZJ-8 homologous region 3 (hr3) increased the expression of luc by over 1,600-fold. Molting hormone of 1.0 ~ 2.0 mg/ml can dramatically down regulate expression of luc. Juvenile hormone analogue of 0.5 ~ 2.0 mg/ml increased expression of luc by 145.8% to 75.7%. Deletion assay revealed that the promoter fragment of 159 bp contains the basal promoter structure; Promoter fragments of 309 bp and 542 bp showed similar but much higher transcriptional activities than that of 159 bp, suggesting that nucleotide from -159 to -309 nt upstream the translation initiation site harbors the main cis-acting elements.

Characterization of Ecdysteroid UDP-Glucosyltransferase Gene Promoter from Bombyx mori Nucleopolyhedrovirus

( Xing Jia Shen ),( Yong Zhu Yi ),( Shun Ming Tang ),( Zhi Fang Zhang ),( Yi Ren Li ),( Jia Lu He ),( Xiang Fu Wu ) 한국잠사학회 2007 International Journal of Industrial Entomology Vol.8 No.2

Bombyx mori nucleopolyhedrovirus(BmNPV) ecdysteroid UDP-glucosyltransferase gene (egt) promoter fragments of different lengths were amplified from BmNPV ZJ-8 genomic DNA by PCR. Reporter plasmids pBmegt542-luc, pBmegt309-luc and pBmegtl59-luc with luciferase (luc) driven by egt promoters were constructed. Both in vitro and in vivo expressions showed that BmNPV egt promoter activity requires the transactivation of viral factor(s), and expression of luc was detected earliest at 24 hrs post infection (pi). BmNPV ZJ-8 homologous region 3 (hr3) increased the expression of luc by over 1,600-fold. Molting hormone of 1.0∼2.0㎍/㎖ can dramatically down regulate expression of luc. Juvenile hormone analogue of 0.5∼2.0㎍/㎖ increased expression of luc by 145.8% to 75.7%. Deletion assay revealed that the promoter fragment of 159 bp contains the basal promoter structure; Promoter fragments of 309 bp and 542 bp showed similar but much higher transcriptional activities than that of 159 bp, suggesting that nucleotide from -159 to -309 nt upstream the translation initiation site harbors the main cis-acting elements.

Increased impact of heat domes on 2021-like heat extremes in North America under global warming

Xing Zhang,Tianjun Zhou,Wenxia Zhang,Liwen Ren,Jie Jiang,Shuai Hu,Meng Zuo,Lixia Zhang,Wenmin Man 한국기상학회 2023 한국기상학회 학술대회 논문집 Vol.2023 No.4