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        A NOVEL ANTI-ESTROGEN RECEPTOR TARGETED WATER-SOLUBLE QDs FOR BREAST CANCER CELL IMMUNOFLUORESCENT LABELING

        XING REN,Jing-Yuan Wang,HAILONG HUANG,ZHELI XU,GUANG SUN 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2014 NANO Vol.9 No.7

        This research is aimed to develop a novel kind of biomarker based on water-soluble quantum dots(QDs) conjugated with estrogen receptor (ER) for the detection of breast cancer (BC). To achievethe purpose, hydrophobic octadecylamine-coated QDs were encapsulated with amphiphilic bio-compatible centipede-like polymer p(aspartate)-Na-graft-p(ethylene glycol)-dodecylamine(PASP-Na-g-PEG-DDA), and then modi¯ed with ER monoclonal antibodies, which is one ofthe most popular biomarkers for the detection and management of breast cancer worldwide. Thetargeted QDs kept the original optical property of QDs. The cytotoxicity in vitro experimentsshowed that QDs-ER probes had no obvious toxic side e®ects. Remarkably, confocal laser scanningmicroscopy (CLSM) revealed that the localized °uorescence appeared around the nucleus of humanbreast cancer cell MCF7 (ER positive) compared with that of human breast cancer cellMBA-MD-231 (ER negative). According to the above analysis, this new kind of probe has greatpotential in the diagnosis of breast tumor in vitro in future clinical application.

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