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Diatomaceous earth를 이용한 경제적이고 효율높은 homemade PCR purification column의 제조 및 평가
이동호,이승관,이창규,조경진,최명재,이국성 高麗大學校 倂設 保健大學 保健科學硏究所 2002 保健科學論集 Vol.28 No.2
In recent years the amount of sequencing has increased tremendously, especially due to the introduction of automated DNA sequencing. PCR is principal method for the synthesis of templates for DNA sequencing. Consequently, the need for PCR cleanup for template preparation has increased. Already, many PCR purification kits are marketed. This DNA purification kits uses most glass membrane or silica gel, So, can do rapidly efficient purification by simple miniprep. But, efficiency of purification kits is different according to manufacturer and is very expensive. Therefore, we compare the homemade Diatomaceous earth column with major manufacturer's spin column methods for DNA purification. As a result, we found that the homemade Diatomaceous earth column method resulted in equal or better yields relative to the commercial purification kits.
박노국,이종욱,이승철,이종대,류시옥,이태진 영남대학교 공업기술연구소 2000 工業技術硏究所論文集 Vol.28 No.2
The zinc titanate sorbents(ZTG40) were prepared and the tests for durability and attrition resistance were carried out to develop the highly effective desulfurization sorbents. In the attrition tests for ZTG40 the results showed that the attrition resistance was 94.08% and that the sulfur capacity was maintained above 15g S/100g sorbent throughout the 100-cycle test, its maximum being 23.05g S/100g sorbent. As compared to sorbents developed by the developed countries, ZTG40 showed superior performances. In the advanced countries 15g S/100g sorbent is a yardstick of excellency for the sulfur capacity of desulfurization sorbents. Therefore, ZTG40 developed in this study has the desirable physical and chemical properties for fluidized desulfurization of coal gases.
21C 부산체육의 활성화를 위한 시설 및 프로그램 연구
이상봉,김용재,서국웅,양점홍 釜山大學校 附設 體育科學硏究所 1996 體育科學硏究所 論文集 Vol.12 No.-
This research investigated Institution and Program for the Development of the Pusan Sports in 21C by examining literatures. Conclusiona from the results as follows. 1. We should provide a long period propulsion project in three stage with planning, diffusion and development stage for the bring development and diffusion of Program. 2. We should establish a specialty research institution, the exclusive responsibility of program's development and research. 3. We should provide various participation programs not that for an elite athlete but that for all nation. 4. Enlarge the same taste club and we must support with various administration and financial. 5. Cultivate a specialty leader and we must take interested in the study and training with continual.
이진하,김진수,김성국 慶北大學校 齒科大學 1990 慶北齒大論文集 Vol.7 No.2
This is a case report of both superior(5mm) & posterior (7mm) repositioning of maxilla. This patient, 25-year old female, was admitted to our Dept. of Oral & Maxillofacial Surgery with chief complaint of lip incompetence & maxillary prognathism after orthodontic treatment. In this case, we performed Le fort Ⅰ osteotomy and ostectomy of maxillary tuberosity region including the perpendicular plate of the palatine bone. This repositioned maxilla is fixed with miniplate and wedge-shaped corticocancellous bone blocks are grafted between the margins of the upper & lower segments. As a result, we achieved improving appearance & lip incompetence.
태권도선수의 체중감량시 補中治濕湯 투여가 신체조성, 혈액생화학적 변인 및 호르몬 변화에 미치는 영향
이승국,문원재,오재근 韓國體育大學校 1997 論文集 Vol.20 No.-
This study was to investigate the effects of Bo-Jung-Chi-Seub-Tang administration on body composition, blood biochemical factors and hormonal changes during 2 weeks Weight Reduction in TaeKwonDo Athletes. 10 subjects were belong to be in elite athletes of KNUPE. They were divided two groups(administration group and non-administration) by 5 subjects in each group randomly. As the results of the experiment, conclusions were made as follows: In terms of % Fat, Fat W.T., Mg, TG, Renin, it was shown significant differences between two groups. Although there were no significant differences, rate of weight reduction and levels of LBM, BUN, total cholesterol, HDL-C, LDL-C in Bo-Jung-Chi-Seub-Tang administration group were changed more higher or lower than non-administration group.
강성진,김광수,이지현,김종국,김민정 고신대학교 의과대학 2009 고신대학교 의과대학 학술지 Vol.24 No.1
A 21-year-old woman developed painful left third, fourth and sixth nerve palsy. The retro-orbital pain and ophthalmoplegia were improved after steroid pulse therapy. After six weeks, during took steroid by orally left facial weakness developed. Neurologic examination showed left peripheral seventh cranial nerve palsy. The Tolosa-Hunt syndrome associated with facial palsy is rare condition. So we report a case of Tolosa-Hunt syndrome preceded by ipsilateral facial palsy
이용훈,최석천,지명국,이상철,정효민,정한식 경상대학교 해양산업연구소 2002 해양산업연구소보 Vol.15 No.-
The purpose of this research is to obtain the heat transfer characteristics of the absorber type solar collector. This new system moving by time and degree. The second system had the solar tracking system. The x-y axis were designed at intervals of 0.33˚/min and 0.16˚/min respectively. In this system the absorber specification was 1OOLX80Φ1.4t. This experiment was conducted from July to August. 2002. The temperature of solar collector was reached about mean temperature 250℃.
李成植,金相烈,金燦國 동아대학교 환경문제연구소 1979 硏究報告 Vol.3 No.1
The purpose of this study is to elucidate the effect of surfactant on oxygen absorption in the bubble column. The experiment was carried out according to some parametars such as temperature(8∼36℃), concentration of surfactant (1.25×10^(-3)∼5×10^(-3)%), air velocity (0.38∼0.96m/sec) and hole diameter(0.7∼0.9㎜)of plate. The equilibrium concentration of dissolved oxygen, overall transfer coefficient and surface tension were investigated by experimental data. The results indicated that equilibrium concentration of dissolved oxygen decreased approximately by 5∼20% on water without surfactant and overall transfer coefficient decreased approximately by 10∼30% on water without surfactant.
혼합형 자가면역성 용혈성 빈혈을 동반한 전신성 홍반성 루푸스 1예
이승철,김선임,이승훈,이현경,조남국,한성훈,유영진,조종래 인제대학교 2000 仁濟醫學 Vol.21 No.2
A 17-year-women developed mixed warm and cold antibody type autoimmune hemolytic anemia associated with systemic lupus erythematosus. The patients was admitted to our hospital for syncope. Jaundice was observed. Urinalysis revealed protein and hemoglobin. Her hemoglobin was 4.6g/dl and reticulocytes were 10%. Total bilirubin was elevated, while haptoglobin and complement were abnormally reduced. ANA and mitochondrial Ab were detected. The direct and indirect Coombs test were positive. The direct antiglobulin test was positive ; IgG and C3d were detected on the red cell surface. The cold agglutinin(CA) titer was 128. The patients was initially treated with high dose steroid therapy. However the patients did not responded to high dose steroid therapy. We treated the patients with plasmapheresis and synchronized cyclophosphamide therapy. Clinical remission of hematologic complications was achieved in this patients. We report a case of mixed type autoimmune hemolytic anemia with SLE that responded plasma pheresis and synchronized cyclophosphamide therapy.
코크스 오븐에서 에너지 효율 향상을 위한 통합연소진단시스템 개발
이지수,이용국,이성우,심재우 한국공업화학회 2004 응용화학 Vol.8 No.1
In making coke through carbonization process, it is possible to produce the required high quality coke in blast furnace and reduce the cost of energy as well as increase the life of coke oven if we maintain the desired coke oven temperature by means of optimal combustion management (CM). In this study, hybrid combustion diagnostic expert system (HCDES) was developed through analyzing the trend and temperature distribution of flues in the oven and combining the domain expert's knowledge. The model also considered and incorporated the characteristics and the structure of the oven. In developing the diagnostic system, a general and accessible expert language, CLIPS (C Language Integrated Production System) was used.