PCR 기법을 이용한 한국재래산양 β-casein 유전자의 특성

김지애,류승희,유성란,이준헌,서길웅,김선균,상병찬 충남대학교 농업과학연구소 2002 농업과학연구 Vol.29 No.2

본 연구는 한국 재래 산양 112두와 유산양인 Saanen종 7두의 혈액으로부터 genomic DNA를 추출하고, PCR-RFLP 방법에 의해 β-casein 유전자의 특성을 분석하여 한국재래산양의 효율적인 유전자원의 보전 및 개량을 위한 기초 자료로 제공하고자 실시하였다. 한국재래산양의 genomic DNA로부터 PCR기법을 이용하여 β-casein의 유전자좌를 증폭한 결과 각각 481bp 크기의 단편이 양호하게 증폭되었음을 확인하였다. β-casein 유전자좌의 증폭산물에 대한 Bal Ⅰ의 제한효소를 처리한 결과, β-casein AB형은 481bp, 284bp 및 197bp의 단편을, 그리고 BB형은 284BP와 197BP의 단편을 한국재래산양과 유산양인 Saanen 종에서 확인 할 수 있었다, 유전자형 빈도에 있어서는 한국재래산양에서 β-casein AB 및 BB의 빈도는 각각 6.25 및 93.75%이었고, 유산양인 Saanen 종은 각각 57.14 및 42.86%이었다. 유전자빈도에 있어서는 한국재래산양의 β-casein A 및 B의 빈도가 각각 0.031 및 0.969이었고, Saanen 종에서는 각각 0.286 및 0.714의 빈도를 보였다 한국재래산양의 β-casein 유전자의 염기서열과 이미 보고되어 있는 goat의 염기서열(GeneBank accession Number M90556)간에는 총 11개의 염기서열에 차이를 나타내어 97.71%의 상동성을 보였다, 따라서 한국재래산양의 β-casein 유전자의 다형성과 염기서열 분석에 의한 분자유전학적 특성의 규명은 한국재래산양의 유전자원의 보전 및 개량을 위한 기초 및 응용 자료로 이용될 수 있을 것으로 생각된다. This study was performed to provide the basic data for preservation and improvement of genetic resources according to finding genetic construction obtained from analysis of genetic characteristics of β-casein gene in Korean Native goat and Saanen using the PCR-RFLP. This study confirmed the amplified products of 481bp fragments obtained from the amplification of β-casein loci by PCR. The β-casein AB genotype showed 481, 284 and 197bp, and β-casein BB genotype showed 284 and 197bp fragments in Korean Native goat and Saanen. The frequencies of β-casein genotype in Korean Native goat were 6.25 and 93.75% for AA and AB and the frequencies of β-casein genotype in Saanen were 57.14 and 42.86% for AA and AB types. The frequencies of β-casein A and B alleles were 0.031 and 0.969 in Korean Native goat and the frequencies of β-casein A and B alleles are 0.286 and 0.714 in Saanen, respectively. The nucleotide sequence of β-casein gene of Korean Native goat was 97.71% higher homology with 11 nucleotide sequences difference of that of goat reported in GeneBank (M90556). Therefore, this study of molecular genetic characteristics by the analysis of genetic polymorphism and sequencing for β-casein gene should be used as basic and applying data for preservation and improvement of genetic resources in Korean Native goat breeding.

복막투석복막염 환자에서 발생한 창자벽공기증 1예

정선영,나지훈,최윤정,고성애,조규향,박종원,도준영,윤경우 영남대학교 의과대학 2009 Yeungnam University Journal of Medicine Vol.26 No.1

Peritonitis is a serious problem in patients undergoing peritoneal dialysis. Rarely pneumatosis intestinalis can occur as a complication of this infectious process. Pneumatosis intestinalis is a potential life threatening condition with a challenging management. The mortality of peritoneal dialysis patients with pneumatosis intestinalis secondary to mesenteric ischemia is almost 100%. We describe a rare case of pneumatosis intestinalis in a peritoneal dialysis patient who developed Staphylococcus aureus peritonitis which was initially treated with appropriate antibiotics. Since initial response to therapy was not achieved, an abdominal computerized tomography was done which revealed a pneumatosis intestinalis. A laparotomy was performed and small bowel necrosis was seen. A segmented resection with ileostomy, jejunostomy was done. Though surgical treatment was performed, the patient died in 2 weeks after admission. Pneumocystitis intestinalis in peritoneal dialysis peritonitis is a uncommon complication which requires prompt evaluation to rule out mesenteric ischemia as it carries a high mortality and its management will be surgical.

갯쑥부쟁이(Heteropappus hispidus (Thunb.))의 감마방사선 감수성과 M1 세대 형질변동 특징

오병권,홍경애,송성준,유장걸,이선주,이영일 제주대학교 방사능이용연구소 2003 연구보고 Vol.17 No.-

제주도에서 자생하는 갯쑥부정이(Heteropappus hispidus(Thunb.))에 감마선을 10, 20, 40, 80, 120, 160, 200, 300, 400Gy 수준으로 나누어서 조사한 뒤 발아율 및 발아 30일 후의 본엽 출현을, LD_(50), 본엽길이, 줄기부분의 안토시아닌 색소 형성 유무, 생육 60일 후의 로젯형 엽, multi-shoot 형성율, 초장, 채화 결실율등의 주요 양적형질들을 생존개체를 대상으로 실시하였다. 120Gy 까지의 발아율은 88.5%로 대조구와 큰 차이를 보이지 않았으나 160Gy 이상에서 부터는 급격히 감소하여 치사선량(LD_(50))은 160Gy 범위 정도였다. 또한, 본엽 출연율과 그 길이는 조사선량이 높아질수록 억제되었다. 안토시아닌 색소 형성은 조사전량이 높을수록 대조구 2%에서 30%로 높아졌으며 로젯형도 고선량 처리구에서 발생하였다. Multi-shooting은 80Gy 이상일 때 부터 나타났으며 multi-shooting을 형성하는 개체들은 초장은 짧고 줄기는 가늘게 다발형으로 자라는 특성이 있어서 만일 형질이 고정된다면 화분 관상용 개체로 선발하기에 적합하였다. 특히, 조사선량이 증가됨에 따라서 안토시아닌 색소 형성, 로젯형 개체, multi-shoot등의 개체가 많이 출현되었는데 이는 돌연변이 유기의 선발지표가 될 수 있을 것으로 보인다. The seeds of naturally growing Heteropappus hispidus Thunb were treated by nine different doses(0, 10, 20, 40, 80, 120, 160, 200, 300, 400㏉) of gamma rays to investigate their germination rate and to quantify the characteristics of their germinated plant as like as leaf appearance and length, the formation rate of anthocyanin color in stem 30 days after germination, the formation rate of rosette leaf and multi-shoot, the flowering and seed-bearing, and shoot length. The germination rate at least up to 120 ㏉ was not greatly affected but was rapidly decreased at over 160 ㏉. It seemed that lethal dose(LD_(50)) of germination was 160 ㏉. The leaf appearance and growth was also inhibited, but the formation rate of anthocyanin color in the flower stem was enhanced up to 30% with dose. The rosette plants were observed in plants irradiated with higher than 40 ㏉. Multi-shoots were developed over 80 ㏉. For a short shoot length and bundle of thin stem, it was considered that they can be selected as the potential pot flower plants, through genetic fixation. In particular, it was suggested that the formation of anthocyanin color in flower stem, rosette and multi-shoot plants induced by the morphologically markers for the mutant selection of Heteropappus hispidus (Thunb.)

갯쑥부쟁이(Heteropappus hispidus (Thunb.))의 감마방사선 감수성과 M1 세대 형질변동 특징

오병권,홍경애,송성준,유장걸,이선주,이영일 한국환경농학회 2003 한국환경농학회지 Vol.22 No.4

제주도에서 자생하는 갯쑥부쟁이(Heteropappus hispidus (Thunb.))에 감마선을 10, 20, 40, 80, 120, 160, 200, 300, 400 Gy 수준으로 나누어서 조사한 뒤 발아율 및 발아 30일 후의 본엽 출현율, LD_(50), 본엽길이, 줄기부분의 안토시아닌 색소 형성 유무, 생육 60일 후의 로젯형 엽, multi-shoot 형성율, 초장, 개화 결실률 등의 주요 양적형질들을 생존개체를 대상으로 실시하였다. 120 Gy 까지의 발아율은 88.5%로 대조구와 큰 차이를 보이지 않았으나 160 Gy 이상에서 부터는 급격히 감소하여 치사선량(LD_(50))은 160 Gy 범위 정도였다. 또한, 본엽 출현율과 그 길이는 조사선량이 높아질수록 억제되었다. 안토시아닌 색소 형성은 조사선량이 높을수록 대조구 2%에서 30%로 높아졌으며 로젯형도 고선량 처리구에서 발생하였다. Multi-shooting은 80 Gy 이상일 때 부터 나타났으며 multi-shooting을 형성하는 개체들은 초장은 짧고 줄기는 가늘게 다발형으로 자라는 특성이 있어서 만일 형질이 고정된다면 화분 관상용 개체로 선발하기에 적합하였다. 특히, 조사선량이 증가됨에 따라서 안토시아닌 색소 형성, 로젯형 개체, multi-shoot등의 개체가 많이 출현되었는데 이는 돌연변이 유기의 선발지표가 될 수 있을 것으로 보인다. The seeds of naturally growing Heteropappus hispidus (Thunb.) were treated by nine different doses (0, 10, 20, 40, 80, 120, 160, 200, 300, 400 ㏉) of gamma rays to investigate their germination rate and to quantify the characteristics of their germinated plants as like as leaf appearance and length, the formation rate of anthocyanin color in stem 30 days after germination, the formation rate of rosette leaf and multi-shoot, the flowering and seed-bearing, and shoot length. The germination rate at least up to 120 ㏉ was not greatly affected but was rapidly decreased at over 160 ㏉. It seemed that lethal dose (LD_(50)) of germination was 160 ㏉. The leaf appearance and growth was also inhibited, but the formation rate of anthocyanin color in the flower stem was enhanced up to 30% with dose. The rosette plants were observed in plants irradiated with higher than 40 ㏉. Multi-shoots were developed over 80 ㏉. For a short shoot length and bundle of thin stem, it was considered that they can be selected as the potential pot flower plants, through genetic fixation. In particular, it was suggested that the formation of anthocyanin color in flower stem, rosette and multi-shoot plants induced by the high dose of gamma rays could be utilized as the morphological markers for the mutant selection of Heteropappus hispidus (Thunb.).

주의력결핍과잉행동장애와 Alpha-1C-Adrenergic 수용체 유전자와의 연관성에 대한 연구

조수철,김재원,김붕년,황준원,박미라,김순애,조대연,유희정,정운선,손정우,박태원 大韓神經精神醫學會 2008 신경정신의학 Vol.47 No.1

Objectives : Neurobiological and pharmacological research has suggested that the dysregulation of the central noradrenergic systems might be involved in the pathophysiology of ADHD. The aim of this study was to examine the association of the alpha-1C-adrenergic receptor gene (ADRA1C) with ADHD in Korean subjects. Methods : In a case-control study, we assessed 186 DSM-IV ADHD probands and 150 normal controls. One hundred and eight trios were studied in a family-based association analysis. Psychiatric diagnoses were derived through structured diagnostic interviews. For the clinical evaluation of the ADHD subjects, the Child Behavior Checklist (CBCL), the ADHD Rating Scale-IV (ARS) and the Junior Temperament and Character Inventory (JTCI) were administered. A computerized continuous performance test (CPT) was used to measure the inattention and impulsivity of the ADHD children. Results : There were no significant differences in the genotype or allele frequencies of the ADRA1C PstI polymorphism between the ADHD and control group (p>0.05). The transmission disequilibrium test (TDT) analysis observed no evidence for biased transmission of any of the alleles of the PstI polymorphism. There were no significant differences in the CPT or JTCI profiles between those ADHD subjects with the CC genotype and those with the other (CT+TT) genotypes at the PstI polymorphism. Conclusion : The results of this study do not support the ADRA1C as a major genetic susceptibility factor in ADHD.

정신분열병의 유전적 고위험군에서 인지, 정서 및 사회기능 연구

이경진,위휘,유소영,이애라,송지연,하태현,홍경수,김명선,권준수 大韓神經精神醫學會 2006 신경정신의학 Vol.45 No.1

Objectives : Vulnerability marker in biological relatives of schizophrenia may be very useful in identifying the propensity for the development of schizophrenia. This study suggests that the relatives at risk for genetically developing schizophrenia have impairments in several domains of cognition, social function and affective function as a vulnerability marker. Methods : A neurocognitive function test, a social function test and an affective function test were administered to fifteen healthy relatives from families with two or more patients with schizophrenia (geneticallly high risk subjects). Fifteen healthy controls matched for age and gender were recruited in this study. Results : Compared to the control subject, the genetic high risk subjects performed significantly more poorly in the selective attention and recall memory of neurocognitive function. The high dsk subjects also had lower scores in independence-performance and independence-competence of the social function test and emotion control of the affective function test. Conclusion : Selective attention, recall memory, independence-performance, independence-competence and emotion control ability may be a valuable marker for genetic study of schizophrenia.

Functional Expression of Anti-hepatitis B Virus (HBV) preS2 Antigen scFv by cspA Promoter System in Escherichia coli and Application as a Recognition Molecule for Single-walled Carbon Nanotube (SWNT) Field Effect Transistor (FET)

Jun, Sun-Ae,Nam, Dong-Hyun,Lo, Young-Seob,Kim, Ju-Jin,Chang, Hyun-Ju,Lee, Jeong-O,Kim, Yong-Hwan,Sang, Byoung-In 한국생물공학회 2010 Biotechnology and Bioprocess Engineering Vol.15 No.5

The preS2 antigens of hepatitis B virus (HBV), which causes a serious health problem in the world, have been implicated in hepatocyte cell binding and viral penetration. Therefore, the importance of antibody production against preS2 antigen for early diagnosis of HBV has been well established. In this study, the recombinant HBV preS2 single chain variable fragment (scFv) antibody was successfully expressed in E. coli with the novel cold shock vector (pCold) under the cspA promoter, and its expression level was compared with the pET vector under the T7 promoter. Additionally, a host with an oxidizing cytoplasm, E. coli trxB/gor double mutant, was used to improve the soluble expression. The anti-HBV preS2 scFv using pCold vector was successfully expressed in a soluble and functional form in both wild type and double mutant E. coli, while the scFv using the pET vector was expressed in an insoluble form in spite of using a double mutant providing an oxidizing environment. The induction with 0.05 mM IPTG showed a 2-fold higher functional expression compared to induction with 1 mM IPTG, and the functional expression at the induction temperature ($15^{\circ}C$), which is optimal temperature for pCold vector, was improved 2-fold and 3-fold at 4 and $25^{\circ}C$, respectively. The efficacy of anti-HBV preS2 scFv for detecting HBV preS2 antigen was tested and verified by using Ni-decorated single-walled carbon nanotube (SWNT) field effect transistors.

The production of 1,3-propanediol by Klebsiella pneumonia using crude glycerol

Sun-Ae Jun,Cheol-Hee Kang,Yong Hwan Kim 한국생물공학회 2008 한국생물공학회 학술대회 Vol.2008 No.4

생물화공 : 글리세롤로부터 1,3-propanediol 생산을 위한 Klebsiella pneumoniae 배양 조건 최적화

전선애 ( Sun Ae Jun ),공성욱 ( Sean W Kong ),상병인 ( Byoung In Sang ),엄영순 ( Young Soon Um ) 한국화학공학회 2009 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.47 No.6

바이오디젤 부산물인 폐글리세롤을 이용한 생물학적 1,3-propanediol 생산

전선애(Sun-Ae Jun),강철희(Cheol-Hee Kang),공성욱(Sean W Kong),상병인(Byoung-In Sang),엄영순(Youngsoon Um) 한국생물공학회 2008 KSBB Journal Vol.23 No.5