Evaluation of Non-isothermal Melt Crystallization Kinetics of the Poly(lactic acid)/Graphene Composites under Variant Cooling Circumstances via Solidification Analysis Approach

Bin Yang,Peng Zhang,Shu-Chun Zhao,Ru Xia,Li-Fen Su,Ji-Bin Miao,Peng Chen,Jia-Sheng Qian,You Shi 한국고분자학회 2019 폴리머 Vol.43 No.5

The poly(lactic acid) (PLA)/graphene (GN) composites were prepared by solution blending. An in situ measurement technique was adopted to examine the non-isothermal crystallization of PLA/GN composites, which was different from the typical circumstances at constant cooling rate in DSC. The rheological properties of the samples were investigated, and the Carreau-A model was applied to non-linearly fit the rheological data. The non-isothermal crystallization kinetics and solidification behaviors at variant cooling rates were investigated using a modified version of Avrami equation by Jeziorny coupled with three-parameter model (TPM) and four-parameter model (FPM). Our findings showed that the viscosity of the PLA/GN composites dropped with increasing shear rate or GN content. Crystallization kinetic analysis suggested that GN served as an effective nucleating agent for PLA under non-isothermal melt crystallization conditions with variant cooling rate.

SOX12 Promotes Thyroid Cancer Cell Proliferation and Invasion by Regulating the Expression of POU2F1 and POU3F1

Zhenxi Su,Wenqing Bao,Guanghua Yang,Jianping Liu,Bin Zhao 연세대학교의과대학 2022 Yonsei medical journal Vol.63 No.6

Purpose: SOX12 is overexpressed in many cancers, and we aimed to explore the biological function and mechanism of SOX12 inthyroid cancer. Materials and Methods: We first analyzed the expression of SOX12 in thyroid cancer using data in The Cancer Genome Atlas. Immunohistochemistryand qRT-PCR were performed to identify SOX12 expression in thyroid cancer tissue and cells. Thyroid cancercells were transfected with small interfering RNA targeting SOX12, and cellular functional experiments, including CCK8, woundhealing, and Transwell assays, were performed. Protein expression was examined by Western blot analysis. A xenograft model wasdeveloped to evaluate the effect of SOX12 on tumor growth in vivo. Results: SOX12 expression was increased in thyroid cancer tissue and cells. SOX12 promoted cell proliferation, migration, and invasionand accelerated tumor growth in vivo. The expression of PCNA, Cyclin D1, E-cadherin, Snail, MMP-2, and MMP-9 was affectedby SOX12 knockdown. Bioinformatic analysis showed that SOX12 could interact with the POU family. SOX12 knockdown inhibitedthe expression of POU2F1, POU2F2, POU3F1 and POU3F2, and SOX12 expression showed a positive correlation with POU2F1,POU3F1, and POU3F2 expression in clinical data. POU2F1 and POU3F1 were able to reverse the effect of SOX12 knockdown onthyroid cancer cells. Conclusion: SOX12 affects the progression of thyroid cancer by regulating epithelial-mesenchymal transition and interacting withPOU2F1 and POU3F1, which may be novel targets for thyroid cancer molecular therapy.

Effects of Zn Addition on Dynamic Recrystallization of High Strain Rate Rolled Al–Mg Sheets

Lu Zhao,Weijun Xia,Hongge Yan,Jihua Chen,Bin Su 대한금속·재료학회 2022 METALS AND MATERIALS International Vol.28 No.5

Effects of Zn addition (0, 0.5, 1.0 and 1.5 wt%) on dynamic recrystallization (DRX) behavior of Al-9.2Mg alloy during highstrain rate rolling (with rolling temperature of 400 °C and strain rate of 8.6 s−1) are carefully investigated. The Zn contentplays a significant role in the enhanced DRX of Al-9.2Mg alloy. The critical strain (εc) and the peak strain (εp) are reducedfrom 0.178 ± 0.002 to 0.102 ± 0.001 and 0.211 ± 0.003 to 0.139 ± 0.002 with the Zn content increasing from 0 to 1.5%,respectively. Almost complete DRX grains are obtained in the Al-9.2Mg-0.5Zn alloy. The DRX volume fraction (FDRX), theaverage DRX grain size (AGS), the volume fraction of high-angle grain boundaries (FHAGBs) and the average grain boundaryangle (θAV) are 91.5%, 5.6 μm, 91.1% and 37.6 º, respectively. The Zn addition can enhance the formation of deformationbands (DBs) and promote the DRX process by providing the nucleation sites for new grains, while the increased dynamicprecipitates inhibit DRX. Continuous dynamic recrystallization (CDRX) is the dominant DRX mechanism, while discontinuousdynamic recrystallization (DDRX) is also existent. Effects of Zn addition on DRX process of the Al–Mg alloys canbe ascribed to the more DBs, the reduced stacking fault energy (SFE) and the dynamic precipitates.

Prognostic Value of PLCE1 Expression in Upper Gastrointestinal Cancer: a Systematic Review and Meta-analysis

Cui, Xiao-Bin,Peng, Hao,Li, Su,Li, Ting-Ting,Liu, Chun-Xia,Zhang, Shu-Mao,Jin, Ting-Ting,Hu, Jian-Ming,Jiang, Jin-Fang,Liang, Wei-Hua,Li, Na,Li, Li,Chen, Yun-Zhao,Li, Feng Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.22

Background: A number of studies have identified a shared susceptibility locus in phospholipase C epsilon 1 (PLCE1) for esophageal squamous cell carcinoma (ESCC) and gastric cardia adenocarcinomas (GCA). However, the results of PLCE1 expression in esophageal and gastric cancer remain inconsistent and controversial. Moreover, the effects on clinicopathological features remain undetermined. This study aimed to provide a precise quantification of the association between PLCE1 expression and the risk of ESCC and GCA through meta-analysis. Materials and Methods: Eligible studies were identified from PubMed, Wanfang Data, ISI Web of Science, and the Chinese National Knowledge Infrastructure databases. Using RevMan5.2 software, pooled odds ratios (ORs) with 95% confidence intervals (CIs) were employed to assess the association of PLCE1 expression with clinicopathological features relative to ESCC or GCA. Results: Seven articles were identified, including 761 esophageal and gastric cancer cases and 457 controls. Overall, we determined that PLCE1 expression was associated with tumor progression in both esophageal cancers (pooled OR=5.93; 95%CI=3.86 to 9.11) and gastric cancers (pooled OR=9.73; 95%CI=6.46 to 14.7). Moreover, invasion depth (pooled OR=3.62; 95%CI=2.30 to 5.70) and lymph node metastasis (pooled OR=4.21; 95%CI=2.69 to 6.59) were linked with PLCE1 expression in gastric cancer. However, no significant associations were determined between PLCE1 overexpression and the histologic grade, invasion depth, and lymph node metastasis in esophageal cancer. Conclusions: Our metaanalysis results indicated that upregulated PLCE1 is significantly associated with an increased risk of tumor progression in ESCC and GCA. Therefore, PLCE1 expression can be appropriately regarded as a promising biomarker for ESCC and GCA patients.

On the hydraulic axial thrust of Francis hydro-turbine

Xing-Ying Ji,Xiao-Bin Li,Wen-Tao Su,Xu Lai,Tian-Xiao Zhao 대한기계학회 2016 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.30 No.5

We present a new method for the computation of hydraulic axial thrust of Francis hydro-turbine. This approach combines the computation of axial thrust on the crown, band and blade by using the numerical simulation and calculation of the pressure distribution outside the crown and band based on the theory of fluid mechanics. The axial thrusts are calculated for different flow conditions with various suction head and flow rate. The calculated results are consistent with that of experimental test on the prototype hydro-turbine, and this combination is proven to be an effective way to assess the magnitude of hydraulic axial thrust for hydro-turbine. Also, the suction head of the turbine is shown to have significant influence on the hydraulic axial thrust.

Differences in the biological properties of mesenchymal stromal cells from traumatic temporomandibular joint fibrous and bony ankylosis: a comparative study

Zhang Pei-Pei,Liang Su-Xia,Wang Hua-Lun,Yang Kun,Nie Shao-Chen,Zhang Tong-Mei,Tian Yuan-Yuan,Xu Zhao-Yuan,Chen Wei,Yan Ying-Bin 한국통합생물학회 2021 Animal cells and systems Vol.25 No.5

The aim of this study was to compare the functional characteristics of mesenchymal stromal cells (MSCs) from a sheep model of traumatic temporomandibular joint (TMJ) fibrous and bony ankylosis. A sheep model of bilateral TMJ trauma-induced fibrous ankylosis on one side and bony ankylosis on the contralateral side was used. MSCs from fibrous ankylosed callus (FAMSCs) or bony ankylosed callus (BA-MSCs) at weeks 1, 2, 4, and 8 after surgery were isolated and cultured. MSCs derived from the bone marrow of the mandibular condyle (BM-MSCs) were used as controls. The MSCs from the different sources were characterized morphologically, phenotypically, and functionally. Adherence and trilineage differentiation potential were presented in the ovine MSCs. These cell populations highly positively expressed MSC-associated specific markers, namely CD29, CD44, and CD166, but lacked CD31 and CD45 expressions. The BA-MSCs had higher clonogenic and proliferative potentials than the FA-MSCs. The BA-MSCs also showed higher osteogenic and chondrogenic potentials, but lower adipogenic capacity than the FA-MSCs. In addition, the BA-MSCs demonstrated higher chondrogenic, but lower osteogenic capacity than the BM-MSCs. Our study suggests that inhibition of the osteogenic and chondrogenic differentiations of MSCs might be a promising strategy for preventing bony ankylosis in the future.

Cordblood-Based High-Throughput Screening for Deafness Gene of 646 Newborns in Jinan Area of China

Shou-Xia Li,Ding-Li Chen,Su-Bin Zhao,Li-Li Guo,Hai-Qin Feng,Xiao-Fang Zhang,Li-Li Ping,Zhi-Ming Yang,Cai-Xia Sun,Gen-Dong Yao 대한이비인후과학회 2015 Clinical and Experimental Otorhinolaryngology Vol.8 No.3

Objectives. Infants with slight/mild or late-onset hearing impairment might be missed in universal newborn hearing screening (UNHS). We identified the mutation hot spot of common deaf gene in the newborns in Jinan area population by screening the mutation spot with neonate cord blood, in order to make clear whether the neonate cord blood for screening is feasible. Methods. Six hundred and forty-six newborns were subjected to both UNHS and genetic screening for deafness by using neonate cord blood. The newborn genetic screening targeted four deafness-associated genes, which were commonly found in the Chinese population including gap junction beta-2 protein (GJB2), gap junction beta-3 protein (GJB3), solute carrier family 26 member 4 (SLC26A4), and mtDNA 12S rRNA. The most common 20 spot mutations in 4 deaf genes were detected by MassARRAY iPLEX platform and mitochondrial 12S rRNA A1555G and C1494T mutations were sequenced using Sanger sequencing. Results. Among the 646 newborns, 635 cases passed the UNHS and the other 11 cases (1.7%) did not. Of the 11 failures, two cases were found to carry homozygous GJB2 p.R143W pathogenic mutation, one case was found to have heterozygous GJB2 235delC mutation, and another one case carried heterozygous GJB3 p.R180X pathogenic mutation. Six hundred and thirty-five babies passed the newborn hearing screening, in which 25 babies were identified to carry pathogenic mutations, including 12 heterozygotes (1.9%) for GJB2 235delC, eight heterozygotes (1.3%) for SLC26A4 IVS7-2A>G, one heterozygote (0.2%) for p.R409H, two homozygotes (0.3%) for m.1494C>T, and two homozygotes (0.3%) for m.1555A>G. Conclusion. Newborn genetic screening through the umbilical cord blood for common deafness-associated mutations may identify carriers sensitive to aminoglycoside antibiotic, and can effectively prevent or delay hearing loss occurs.

Associations of Plasma Glucagon Levels with Estimated Glomerular Filtration Rate, Albuminuria and Diabetic Kidney Disease in Patients with Type 2 Diabetes Mellitus

Hua-Xing Huang,Liang-Lan Shen,Hai-Yan Huang,Li-Hua Zhao,Feng Xu,Dong-Mei Zhang,Xiu-Lin Zhang,Tong Chen,Xue-Qin Wang,Yan Xie,Jian-Bin Su 대한당뇨병학회 2021 Diabetes and Metabolism Journal Vol.45 No.6

Background: Type 2 diabetes mellitus (T2DM) is characterized by elevated fasting glucagon and impaired suppression of postprandial glucagon secretion, which may participate in diabetic complications. Therefore, we investigated the associations of plasma glucagon with estimated glomerular filtration rate (eGFR), albuminuria and diabetic kidney disease (DKD) in T2DM patients.Methods: Fasting glucagon and postchallenge glucagon (assessed by area under the glucagon curve [AUCgla]) levels were determined during oral glucose tolerance tests. Patients with an eGFR <60 mL/min/1.73 m2 and/or a urinary albumin-to-creatinine ratio (UACR) ≥30 mg/g who presented with diabetic retinopathy were identified as having DKD.Results: Of the 2,436 recruited patients, fasting glucagon was correlated with eGFR and UACR (r=–0.112 and r=0.157, respectively; P<0.001), and AUCgla was also correlated with eGFR and UACR (r=–0.267 and r=0.234, respectively; P<0.001). Moreover, 31.7% (n=771) presented with DKD; the prevalence of DKD was 27.3%, 27.6%, 32.5%, and 39.2% in the first (Q1), second (Q2), third (Q3), and fourth quartile (Q4) of fasting glucagon, respectively; and the corresponding prevalence for AUCgla was 25.9%, 22.7%, 33.7%, and 44.4%, respectively. Furthermore, after adjusting for other clinical covariates, the adjusted odds ratios (ORs; 95% confidence intervals) for DKD in Q2, Q3, and Q4 versus Q1 of fasting glucagon were 0.946 (0.697 to 1.284), 1.209 (0.895 to 1.634), and 1.521 (1.129 to 2.049), respectively; the corresponding ORs of AUCgla were 0.825 (0.611 to 1.114), 1.323 (0.989 to 1.769), and 2.066 (1.546 to 2.760), respectively. Additionally, when we restricted our analysis in patients with glycosylated hemoglobin <7.0% (n=471), we found fasting glucagon and AUCgla were still independently associated with DKD.Conclusion: Both increased fasting and postchallenge glucagon levels were independently associated with DKD in T2DM patients.

Effect of Heat Treatment on the Microstructure and Properties of High Strain Rate Rolled 7050 Aluminum Alloy

Yang Liao,Hongge Yan,Weijun Xia,Jihua Chen,Bin Su,Xinyu Li,Lu Zhao 대한금속·재료학회 2022 METALS AND MATERIALS International Vol.28 No.4

High strain rate rolling (HSRR) is developed to improve the strength, plasticity and stress corrosion resistance of 7050 aluminumalloy simultaneously. The volume fraction of the small-angle grain boundary in the HSRRed alloy sheet is 89.9%. TEM shows that the presence of dislocations and sub-grains promotes the precipitation of the second phase. The alloy inthe T6 state has the highest strength (622 MPa) and the biggest elongation to rupture (20.6%), but poor corrosion resistance. The over-aged state shows the best corrosion resistance. The RRA state can achieve the tensile strength equivalent to T6 of614 MPa, the highest yield strength of 561 MPa and corrosion resistance comparable to the over-aged state. TEM observationshows the existence of sub-grains and dislocations plays an important role in the nucleation of the second phase. Thehigh strength can be attributed to the higher volume fraction of the precipitates, and the high plasticity can be attributed tothe higher work hardening rate. The better corrosion resistance can be ascribe to the wider precipitate-free zones at the grainboundary and the presence of the larger size second phase particles.

Effect of Mg Content on the Damping Behavior of Al–Mg Alloys

Zhenzhen Li,Hongge Yan,Jihua Chen,Weijun Xia,Bin Su,Lu Zhao,Min Song 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.9

This article investigated the effect of Mg content (4.5, 6.5 and 9.2, in wt%) on the damping capacities of Al–Mg alloys. The results indicate that the damping behavior can be divided into three regions. Region I refers to the low strain amplituderegion (ε < 5 × 10−5), where the damping capacity decreases with increasing the Mg content and has almost no relation withthe strain amplitude. Region II is the middle strain amplitude region (5 × 10−5 < ε < 8 × 10−4), where the damping capacityincreases rapidly with the strain. Region III refers to the high strain amplitude region (8 × 10−4 < ε < 2 × 10−3), where thedamping capacity remains constant and is independent of the strain when the strain is high enough, but increases with the Mgcontent. The damping values Q−1 of Al–4.5Mg, Al–6.5Mg and Al–9.2Mg alloys are 0.01501 ± 0.00032, 0.01633 ± 0.00032and 0.01862 ± 0.00119 at the strain of 1 × 10−3, respectively. The damping capacity in Region I is mainly determined bythe lattice distortion caused by Mg addition and the restoring force caused by pinning points and Suzuki segregation. Theextended dislocations break away from the pinning effect of Mg atoms and become moveable in Region II, and the movementof extended dislocations is the dominant damping mechanism in Region III.