Effect of Non-animal-Derived Nitrogen Sources on the Production of Hyaluronic Acid by Streptococcus sp. KL0188

Lee, Gil-Yong,Ha, Suk-Jin,Jung, Jong-Hyun,Seo, Dong-Ho,Park, Jong-Yul,Kim, Su-Rin,Park, Nam-Woo,Kweon, Dong-Keon,Park, Sang-Hoo,Park, Cheon-Seok The Korean Society for Applied Biological Chemistr 2009 Applied Biological Chemistry (Appl Biol Chem) Vol.52 No.3

Hyaluronic acid (HA) is a linear high-molecular-weight polysaccharide with useful biomedical applications. Streptococcus zooepidemicus, a typical HA-producing bacterium, requires an animal-derived nitrogen source such as tryptone, peptone or sheep blood as a nutrient. Sixteen non-animal-derived (NAD) nitrogen sources were tested as a replacement for the expensive animal-derived nitrogen sources, which may have safety issues. Among the sixteen tested NAD nitrogen sources, a yeast-derived nitrogen source (YE 0251) showed the highest HA productivity, which was equivalent to the control HA production medium containing tryptone in a 5-L jar and in 3,000-L industrial fermentations. In the 3,000-L fermentation, YE 0251 increased cell mass (dry cell weight) and HA production by 11% and 8%, respectively, compared with the control HA production medium. The fmal specific volumetric productivity (0.41 g/L h) was improved by about 70% after reducing the fermentation time from 20 h to 12 h, compared to the conventional production medium.

텍스트 종류와 Cattell-Horn-Carroll(CHC) 인지요인과의 관계

김수연 ( Su Yhen Kim ),하대현 ( Dae Hyun Ha ) 한국독서학회 2006 독서연구 Vol.0 No.16

초등학교 아동을 대상으로 연구한 보고들을 살펴보면 이야기 텍스트 이해를 설명적 텍스트 이해보다 더 쉬워한다. 이런 차이는 주로 배경지식에 의한 것으로 해석되나 텍스트 이해는 개인의 인지 능력의 영향을 받으며, 인지적 능력의 부족이 이해 능력에 어려움을 가져온다. 본 연구에서는 텍스트의 종류에 따라 어떤 인지적 요인들이 관여하는지를 새로운 지능이론의 관점으로 알아보고자 한다. 본 연구에서 사용된 지능 검사는 현대의 지능이론으로 새로이 제안되는CHC(Cattell-Horn-Carroll) 지능 이론에 따르며 본 연구에서 사용된CHC 지능검사의 신뢰도는 .60-.79이며, 타당도 검증을 위한 탐색적 요인분석과 확인적 요인분석을 실시하여 타당도를 확인하였다. 개인내의 이야기 텍스트를 설명적 텍스트보다 더 이해하기 쉬워한다는 것을 통계적으로 확인하였다. 개인 간 차이로 이야기 텍스트를 더 잘 이해하는 집단과 설명적 텍스트를 더 잘 이해하는 집단 간의 인지적 차이를 살펴보았다. 이야기 텍스트 이해를 잘하는 집단이 Gc(결정지능)와 Ga(청각처리)는 p<.05에서 의미 있는 차이가 있었으며 Gsm(단기기억)은p<.01에서 의미 있게 높은 점수를 보여주었다. 이런 결과는 어려서의 읽기 경험은인지적 요인에 영향을 미치며, 이야기 텍스트를 어려서부터 접하고 경험한 아동의 Gc, Ga, Gsm이 높아질 수 있다는 것을 시사하여 준다. The present study was to explore the relationship between the narrative/expository texts and CHC cognitive ability in 4,5,6 grade elementary school. They were easier to understand the narrative texts than the expository, because of they had not only prior knowledge but also cognitive ability related text comprehension. On the view of recent CHC cognitive ability, this study investigated two types(narrative/ expository) of texts related cognition. The measures of CHC had high reliability and good validity - exploratory, confirmatory factor analysis. The subjects of this study were divided to two groups. The first group was more comprehensible to understand narrative text than expository text. The second group was more comprehensible to understand expository text than narrative text. Between two groups was verified from t-test. The results of this study clearly indicated that the first group was more significant to effect on Gc(comprehension knowledge), Ga(auditory processing) and Gsm (short-term memory) play an important role than the second group.

비만을 동반한 위암 환자에서 두 병을 같이 수술 치료한 경험(증례)

최경현,윤기영,문형환,신연명,서경원,안수미,송윤미,석정희,정경연,이은하 고신대학교의과대학 2008 고신대학교 의과대학 학술지 Vol.23 No.3

Obesity is growing problem in Korea. We had a case of bariatric surgery during gastric cancer operation. Patient was 29 year old Korean lady with early gastric cancer located in the lesser curvature side of the middle 1/3 of the stomach. Her body weight was 89 kg, height 163 cm, and thus body mass index was 33.5 kg/m2. Preoperative blood pressure was 130/90 mmHg, hemoglobin 12.9 g/dL, total lymphocyte count 3,280/mL, serum albumin 4.3 g/dL, CEA 1.1 ng/mL, CA 19-9 9.1 ng/mL, and alpha fetoprotein 2 ng/mL. Another associated disease was right thyroid follicular neoplasm. The chest X-ray was normal. After IM injection of 2,500 units of heparin 30 minutes before the induction of general anesthesia, she received curative subtotal gastrectomy and Roux en Y gastrojejunostomy when a malabsorption loop of 120 cm jejunum was incorporated between the Treitz ligament and the end to side jejuno-jejunostmy site at May 29th 2006. Her postoperative course was uneventful except a minor wound seroma and the elevations of serum amylase(up to 4 folds) and lipase(up to 2 folds). She lost her body weight 9 kg in 5th, 21 kg in 7th, and 27 kg in 10th postoperative months to became 61.5 kg. On follow up exams in July 2008, she gained 4 kg to overcome her weakness and fatigue. From the above result, the incorporation of a malabsorption loop during reconstruction phase of gastric cancer operation was a good option for obese gastric cancer patients especially in cases of early gastric cancer.

상황버섯(Phellinus linteus)으로부터 Ergosterol의 분리

류하나,유종수,송명종,이대영,김동현,노영덕,김인호,백남인 경희대학교식량자원개발연구소 2007 硏究論文集 Vol.26 No.1

상황버섯을 80% MeOH 용액으로 추출하고, 추출물을 EtOAc, n-BuOH 및 물로 분배, 추출하였다. 이 중 EtOAc 분획을 silica gel 및 octadecylsilica gel(ODS) column chromatography로 정제하여 1종의 sterol 화합물을 분리하였다. 화합물의 화학구조는 NMR, MS 및 IR 등의 스펙트럼 데이터를 해석하여, ergosta-7,22E-diene-3β,5α,6β-triol(cerevisterol, 1)로 동정하였다. 이 화합물은 상황버섯에서 처음 분리 보고 되었다. Phellinus linteus was extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with EtOAc, n-BuOH and H_(2)O. The repeated silica gel and ODS column chromatographies of the EtOAc fraction led to the isolation of one sterol. From the result of spectral data including NMR, MS and IR, the chemical structure of the sterol was determined as ergosta-7,22E-diene-3β,5α,6β-triol (cerevisterol, 1). Compound 1 was isolated from the Phellinus linteus for the first time in this study.

미량 Ni 첨가 Fe-Ni 합금의 고온산화 거동에 관한 연구

孫根洙,尹在弘,金晋夏,金顯洙,成田敏夫,林重成 대한금속재료학회 2002 대한금속·재료학회지 Vol.40 No.10

Oxidation behavior of Fe-Ni alloys added by small amount of Ni was studied, under the exposure to the temperatures up to 1100℃, 1150℃, and 1200℃, respectively. The slope of mass change curve representing an oxidation rate was 1 or 2 up to 20 seconds and decreased to 1/2 after 20 seconds in a parabolic shape. The temperature of the initial stage of oxidation increased more quickly at the furnace inside than at the surface of the specimen, and its behavior was reversed due to oxidation heat after 20 seconds later. Oxide layer was mainly composed of FeO. And particle of Fe_3O_4 and Fe_2O_3 was distributed in FeO layer. The order of the composition ratio for the oxide was Fe_2O_3,·Fe_3O_4,·FeO at the oxide surface, FeO,·Fe_3O_4,·Fe_2O_3 at the internal oxide, and FeO,·Fe_3O_4,·Fe_2O_3 at the surface of metal after stripping oxide layer.

蒼耳子에 대한 면역글로블린E 생성억제 효과

宋昊埈,河顯秀,金台現,辛民敎 대한본초학회 1998 大韓本草學會誌 Vol.13 No.2

We studies inhibitation effect of IgE release in the human B cell line(U266B1) and in the serum and spleen cell of mouse that has been immunized by antigen in order to find antiallergic effect of Fructus Xanthii (FX). We have some findings as below. 1. IgE release in serum was inhibited dose dependently in case of administering FX orally in the mouse immunized. 2. IgE release in serum was inhibited dose dependently in case of injecting FX into abdomen in the mouse immunized. 3. When we take LPS+IL-4 way in the mouse spleen cell, IgE release was less than LPS way. 4. When we take LPS+IL-4 way in the human B cell line (U266B1), IgE release was less than LPS way.

부산 일부 대학교 학생들의 니코틴 의존도와 구취의 연관성

김유린(외 5명) 신라대학교 자연과학연구소 2012 自然科學論文集 Vol.22 No.-

The purpose of this study is to examine the association of bad breath and nicotine dependency. By use of the findings from this study, This study conducted the survey of 53 smoker who visited dental hygiene lab for delivery from October to November 2012 with structured questionnaires(Fagerstrom Tolerance Questionnaire FTQ) and equipment(CO measuring instrument, Oral Chroma etc). The data were analyzed by using SPSS 20.0 program for χ2-test, ANOVA. Smokers' average age was 23 years old. The more higher nicotine dependence the more the average age was higher. The more higher nicotine dependence the less to nque brushing. No association of nicotine dependence and self-bad breath level. CO State was Significant differences(ND1(M=2.58), ND2(M=3.98), ND3(M=7.41), ND4(M=4.19), ND5(M=13.14))(F=2.616, p=.047). Dimethyl Sulfide was Significant differences(F=3.026, p=.026).

무선 애드 혹 기반 액티브네트워크상에서 QoS지원에 관한 연구

李基源,裵振勝,丁燦赫,金玄郁,李光培,姜景仁,朴京培,文泰洙,郭承郁,河載承 明知大學校 産業技術硏究所 2003 産業技術硏究所論文集 Vol.22 No.-

Abstract - In this thesis, we propose a QoS supporting method on wireless ad hoc-based active network for improving reliability. On the loss of route path, the existing DSR routing protocol has several problems with wireless network communication because of decease of data reception rate and increase of network load. The proposed method improves reliability of data transfer by using functions of active network. That is, by using functions of active nodes and active packets, the proposed method supports QoS with reservation transfer method that saves data packets on transfer at intermediate nodes with lost path and then re-transmit the packets after route reconfiguration.

말단 비대증의 임상적 특성

박은미,안용환,김현정,김병수,임종주,김하영,박병현,조정구 圓光大學校 醫科學硏究所 2004 圓光醫科學 Vol.19 No.2

랫드에서 인체 재조합 적혈구 조혈인자, rHuEPO의 13주 정맥투여 아만성독성에 관한 연구

김형식,곽승준,천선아,박현선,한하수,임소영,안미영,김원배,김병문,안병옥,홍성렬,이병무 성균관대학교 약학연구소 1998 成均藥硏論文集 Vol.10 No.1

A recombinant human erythropoietin (rHuEPO) was administered intravenously at dosage levels of 0, 100, 500, and 2500 IU/㎏/day for a period of 13 weeks. There were no observed clinical signs and deaths related to treatment in all groups tested. Decreases in body weight gain and food consumption were observed only in males of 2,500 IU/㎏ group after 2 weeks. In hematological parameters, erythrocyte content, hematocrit values and hemoglobin concentration were dose-dependently increased in rHuEPO treated groups. The ratio between kidney weight and whole body weight was significantly increased in females of 500 and 2,500 IU/㎏ groups. The spleen weight was also increased in both sexes of 500 and 2,500 IU/㎏ groups. However, the absolute weight change of other organs was not observed. In histopathological examinations, the renal tubular basophilia was observed only in males and females of 2,500 IU/㎏ groups. From these results, it is concluded that the no-observed adverse effect level(NOAEL) of rHuEPO is 100 IU/㎏ in rats in the present study.