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- 저자 : Sol-Hee LEE (검색결과 300 건)
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Lee, Min-Hye,Lee, Seung-Hoon,Kim, Jihong,Lee, Sol Yi,Lim, Dae-Hee,Hwang, Kyoungtae,Hwang, Hansu,Jung, Yong Chae,Noh, Yong-Young,Kim, Dong-Yu Elsevier 2017 Carbon Vol.125 No.-
<P>The demand for high-purity semiconducting single-walled carbon nanotubes (sc-SWNTs) has increased considerably with the aim of utilizing their superior properties in a range of future applications. Among post-sorting methods, conjugated polymers have been regarded as one of the candidates to selectively isolate sc-SWNTs with uniform electrical properties. Herein, we demonstrate the ability to selectively sort two types of SWNTs by the two polymers PCTV18T and PC12TV18T, which have different alkyl side-chain densities. PC12TV18T, with a high alkyl chain density, shows great sorting ability for both high-pressure carbon monoxide and plasma-torch-grown SWNTs with a weight ratio of almost 1:1 in toluene solution. In addition, it is found that PC12TV18T selectively sorts sc-SWNTs with the high purity. The chirality and diameters of the enriched sc-SWNTs are further confirmed by Raman spectroscopy and photoluminescence excitation/emission mapping. Finally, we fabricate bottom gate/bottom contact thin-film transistors using the enriched sc-SWNTs as semiconductors to verify the electrical performance. Devices with well-percolated sc-SWNT networks displayed p-dominant properties with average charge-carrier mobilities of 2.05 cm(2) V-1 S-1 and 9.87 cm(2) V-1 S-1 and on/off current ratios of approximately 10(4) and 10(5) for high-pressure carbon monoxide and plasma-torch-grown SWNTs, respectively. (C) 2017 Elsevier Ltd. All rights reserved.</P>
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Homo-succinic acid production by metabolically engineered <i>Mannheimia succiniciproducens</i>
Lee, Jeong Wook,Yi, Jongho,Kim, Tae Yong,Choi, Sol,Ahn, Jung Ho,Song, Hyohak,Lee, Moon-Hee,Lee, Sang Yup Elsevier 2016 Metabolic engineering Vol.38 No.-
<P><B>Abstract</B></P> <P>Succinic acid (SA) is a four carbon dicarboxylic acid of great industrial interest that can be produced by microbial fermentation. Here we report development of a high-yield homo-SA producing <I>Mannheimia succiniciproducens</I> strain by metabolic engineering. The PALFK strain (<I>ldhA</I> <SUP> <I>-</I> </SUP>, <I>pta</I> <SUP> <I>-</I> </SUP>, <I>ackA</I> <SUP> <I>-</I> </SUP> <I>, fruA</I> <SUP> <I>-</I> </SUP>) was developed based on optimization of carbon flux towards SA production while minimizing byproducts formation through the integrated application of <I>in silico</I> genome-scale metabolic flux analysis, omics analyses, and reconstruction of central carbon metabolism. Based on <I>in silico</I> simulation, utilization of sucrose would enhance the SA production and cell growth rates, while consumption of glycerol would reduce the byproduct formation rates. Thus, sucrose and glycerol were selected as dual carbon sources to improve the SA yield and productivity, while deregulation of catabolite-repression was also performed in engineered <I>M. succiniciproducens</I>. Fed-batch fermentations of PALFK with low- and medium-density (OD<SUB>600</SUB> of 0.4 and 9.0, respectively) inocula produced 69.2 and 78.4g/L of homo-SA with yields of 1.56 and 1.64mol/mol glucose equivalent and overall volumetric SA productivities of 2.50 and 6.02g/L/h, respectively, using sucrose and glycerol as dual carbon sources. The SA productivity could be further increased to 38.6g/L/h by employing a membrane cell recycle bioreactor system. The systems metabolic engineering strategies employed here for achieving homo-SA production with the highest overall performance indices reported to date will be generally applicable for developing superior industrial microorganisms and competitive processes for the bio-based production of other chemicals as well.</P> <P><B>Highlights</B></P> <P> <UL> <LI> <I>Mannheimia succiniciproducens</I> was engineered to produce homo-succinic acid. </LI> <LI> Different carbon sources were examined for optimal succinic acid production. </LI> <LI> Catabolic repression deregulation allowed co-fermentation of sucrose and glycerol. </LI> <LI> High productivity and yield of homo-succinic acid were achieved. </LI> <LI> Membrane cell recycling fermentation resulted in productivity of 38.6g/L/h. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
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LEE, HEE JUNG,JO, SUZY,RYU, JEONGHYUN,JEONG, HAN-SOL,LEE, GUEMSAN,RYU, MI HEON,JUNG, MYEONG HO,KIM, HYUNGWOO,KIM, BYUNG JOO SPANDIDOS PUBLICATIONS 2015 MOLECULAR MEDICINE REPORTS Vol.12 No.2
<P>Schisandra chinensis Turcz. fruit is widely used to treat skin diseases. The aim of this study was to determine the anti-inflammatory effects of the methanol extract of S. chinensis (MESC) on 1-fluoro-2,4-dinitrofluorobenzene (DNFB)-induced contact dermatitis (CD) in mice. The effects of MESC on ear thickness and weight, histopathological changes, immune cell filtration and cytokine production were investigated in DNFB-induced CD mice. Topical application of MESC effectively inhibited ear swelling (30 or 300 μg on the left ear, P<0.001; 30 μg on the right ear, P<0.001). MESC also inhibited hyperplasia, spongiosis (100 μg/ear, P<0.05 and 300 μg/ear, P<0.001, respectively) and immune cell infiltration (100 μg/ear, P<0.05; 300 μg/ear, P<0.001) induced by DNFB. In addition, MESC suppressed increases in tumor necrosis factor (TNF)-α levels (100 or 300 μg/ear, P<0.05), interferon (INF)-γ (30 μg/ear, P<0.05; 100 μg/ear, P<0.01; 300 μg/ear, P<0.001), interleukin (IL)-6 (300 μg/ear, P<0.05) and monocyte chemoattractant protein (MCP)-1 (30 μg/ear, P<0.05; 100 μg/ear, P<0.01; 300 μg/ear, P<0.001). These results suggest that the anti-inflammatory effects of MESC are mediated by the reduced production of TNF-α, IFN-γ, IL-6 and MCP-1, and that MESC has potential use for the treatment of inflammatory skin diseases.</P>
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Effect of Seawater on the Technological Properties of Chicken Emulsion Sausage in a Model System
Sol Hee Lee,Juhui Choe,Jong-Chan Kim,Hack Youn Kim 한국축산식품학회 2020 한국축산식품학회지 Vol.40 No.3
The aim of this study was to compare the effect of seawater to that of conventional salt (NaCl) on the technological properties of chicken emulsion sausages in a model system. Chicken sausages were prepared with seawater at three levels (10%, 15%, and 20%) in iced water (10%, 5%, and 0%, respectively) or with iced water (20%) and salt (1.2%). There was no difference in pH values and fat loss from emulsion stability between the two treatments. In general, with an increase in the amount of seawater, the water holding capacity (cooking yield and water loss), protein solubility (total and myofibrillar protein), and viscosity were increased. The addition of 20% seawater induced greater (p<0.05) water holding capacity, protein solubility, and viscosity compared to the control sample treated with salt, which was accompanied by an increase in the level of myosin heavy chain protein of samples with 10% and 20% seawater. Furthermore, addition of at least 15% seawater increased all of the main textural properties except for cohesiveness along with the moisture of sausage, whereas the fat and protein contents were decreased. Based on these results, the addition of ≥15% seawater to chicken breast sausage can induce equivalent or enhanced technological properties to those induced with salt, including water holding capacity, protein solubility, viscosity, and textural properties.
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Sol In Suk,Lee Eun,양현종,Lee Yong Ju,Yum Hye Yung,Lee Mi-Hee,Chu Mi Ae,Moon Hui jeong,김효빈,서주희,Shim Jung Yeon,Ahn Ji Young,Jang Yoon Young,Chung Hai Lee,Chung Eun Hee,Kim Kyunghoon,김봉성,김철홍,Park Yang,신미용 대한소아청소년과학회 2023 Clinical and Experimental Pediatrics (CEP) Vol.66 No.3
Background: Human coronaviruses (HCoV) cause mild upper respiratory infections; however, in 2019, a novel coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), emerged, causing an acute respiratory disease pandemic. Coronaviruses exhibit marked epidemiological and clinical differences.Purpose: This study compared the clinical, laboratory, and radiographic findings of children infected with SARS-CoV-2 versus HCoV.Methods: SARS-CoV-2 data were obtained from the Korea Disease Control and Prevention Agency (KDCA) registry and 4 dedicated coronavirus disease 2019 (COVID-19) hospitals. Medical records of children admitted with a single HCoV infection from January 2015 to March 2020 were collected from 10 secondary/tertiary hospitals. Clinical data included age, sex, underlying disease, symptoms, test results, imaging findings, treatment, and length of hospital stay.Results: We compared the clinical characteristics of children infected with HCoV (n=475) to those of children infected with SARS-CoV-2 (272 from KDCA, 218 from COVID-19 hospitals). HCoV patients were younger than KDCA patients (older than 9 years:3.6% vs. 75.7%; P<0.001) and patients at COVID-19 hospitals (2.0±2.9 vs 11.3±5.3; P<0.001). Patients with SARS-CoV-2 infection had a lower rate of fever (26.6% vs. 66.7%; P<0.001) and fewer respiratory symptoms than those with HCoV infection. Clinical severity, as determined by oxygen therapy and medication usage, was worse in children with HCoV infection. Children and adolescents with SARS-CoV-2 had less severe symptoms.Conclusion: Children and adolescents with COVID-19 had a milder clinical course and less severe disease than those with HCoV in terms of symptoms at admission, examination findings, and laboratory and radiology results.
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Lee Eunyoung,Park Sehee,Choi Jae-Phil,Kim Min-Kyung,Yang Eunmi,Ham Sin Young,Lee Seungjae,Lee Bora,Yang Jeong-Sun,Park Byoung Kwon,Kim Da Sol,Lee So-Young,Lee Joo-Yeon,Jang Hee-Chang,Jeon Jaehyun,Park 대한의학회 2023 Journal of Korean medical science Vol.38 No.8
Background: Information on the effectiveness of nirmatrelvir/ritonavir against the omicron is limited. The clinical response and viral kinetics to therapy in the real world need to be evaluated. Methods: Mild to moderate coronavirus disease 2019 (COVID-19) patients with risk factors for severe illness were prospectively enrolled as a treatment group with nirmatrelvir/ritonavir therapy versus a control group with supportive care. Serial viral load and culture from the upper respiratory tract were evaluated for seven days, and clinical responses and adverse reactions were evaluated for 28 days. Results: A total of 51 patients were analyzed including 40 in the treatment group and 11 in the control group. Faster symptom resolution during hospitalization (P = 0.048) was observed in the treatment group. Only minor adverse reactions were reported in 27.5% of patients. The viral load on Day 7 was lower in the treatment group (P = 0.002). The viral culture showed a positivity of 67.6% (25/37) vs. 100% (6/6) on Day 1, 0% (0/37) vs. 16.7 (1/6) on Day 5, and 0% (0/16) vs. 50.0% (2/4) on Day 7 in the treatment and control groups, respectively. Conclusions: Nirmatrelvir/ritonavir against the omicron was safe and resulted in negative viral culture conversion after Day 5 of treatment with better symptomatic resolution.
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Physicochemical properties analysis of bamboo salt on chicken emulsion sausage
( Sol-hee Lee ),( Gye-woong Kim ),( Hack-youn Kim ) 한국축산학회 2020 한국축산학회지 Vol.62 No.1
Quality characteristics of chicken emulsion sausage manufactured with various levels of NaCl and 9 times heated bamboo salt (0.3%, 0.6%, 0.9%, and 1.2% respectively) were examined. The pH value of chicken emulsion sausage was increase tendency with increasing amount of bamboo salt, on the contrary in case of NaCl sample were decrease tendency with increasing amount of NaCl. Both before and after heating, redness of chicken emulsion sausage with bamboo salt treatments were upward trend with increasing amount of bamboo salt. water holding capacity (WHC) of 1.2% NaCl sample was significantly higher than 0.3%-0.9% (p < 0.05), but 0.9%, 1.2% bamboo salt samples were significantly higher than 0.3, 0.6 % (p < 0.05). Water loss of 1.2% NaCl and 0.9% bamboo salt samples were significantly lower than other treatment (p < 0.05). Protein solubility values significantly increased amount of bamboo salt and NaCl (p < 0.05), and samples of 0.9% NaCl and 0.6% bamboo salt values show similar values. Cooking yield of samples were increased tendency with increasing amount of NaCl and bamboo salt. Also viscosity values of sample containing 1.2% bamboo salt sample showed higher viscosity than other treatments. These results show that containing 1.2% NaCl chicken emulsion sausage and 0.9% bamboo salt chicken emulsion sausage were similar physicochemical properties. Therefore, bamboo salt is suitable for manufacturing chicken emulsion sausage.
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Sol-Hee Lee,Hack-Youn Kim 한국축산식품학회 2020 한국축산식품학회지 Vol.40 No.6
The objective of this study was to compare the effects of various salts on the physicochemical properties of pork emulsion sausages. Pork sausages were prepared using two different salts, sodium nitrite (SN) and sodium chloride (SC), and concentrated seawater (CSW). The CIE L*, CIE a*, and CIE b*, and chroma values of cooked and uncooked sausages with added CSW were significantly higher than those of the sausages with added SC (p<0.05). However, uncooked and cooked sausages with added SN and CSW had similar CIE a* values (p>0.05). The residual NO₂⁻ content of sausages with added CSW was significantly lower than that of sausages with added SN. Addition of CSW to sausages resulted in a higher cooking yield compared to the other treatments (p<0.05). Addition of SC resulted in significantly higher volatile basic nitrogen (VBN) and thiobarbituric acid (TBA) values compared to the other treatments. Furthermore, addition of CSW enhanced important physicochemical properties, including CIE a*, CIE b*, residual nitrite content, cooking yield, VBN, TBA, textural properties, and crosssectional area.
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