Clinical Manifestations and Treatment Outcomes of Eosinophilic Gastroenteritis in Children

Jong Sub Choi,Shin Jie Choi,Kyung Jae Lee,Ahlee Kim,Jung Kyung Yoo,양혜란,문진수,장주영,고재성,강경훈 대한소아소화기영양학회 2015 Pediatric gastroenterology, hepatology & nutrition Vol.18 No.4

Purpose: The aim of the present study was to investigate the clinical features and outcome of eosinophilic gastro-enteritis (EGE) in children.Methods: Our study enrolled 24 children who were diagnosed with EGE from 1993 to 2014 at the Department of Pediatrics, Seoul National University Children’s Hospital. The patients’ clinical manifestations, treatments, and out-comes were reviewed from the medical records.Results: The mean age at diagnosis was 5.3 years. Most patients had gastrointestinal symptoms including diarrhea (54.2%) and abdominal pain (45.8%). Peripheral eosinophilia was present in 91.7% of the patients. Thirteen patients (54.2%) showed anemia, and 15 patients (62.5%) had hypoalbuminemia. EGE was classified as mucosal, sub-serosal, or muscular in 75.0%, 20.8%, and 4.2% of cases, respectively. Three patients showed gastroduodenal ulcers upon endoscopic analysis. A history of allergy was reported in 13 patients, including atopic dermatitis, allergic rhinitis, and asthma. Five patients (20.8%) improved with food restrictions. Among the 19 patients treated with steroids, 11 (57.9%) discontinued steroid treatment without subsequent relapse, 4 (21.1%) relapsed after ceasing steroid treat-ment, and 4 (21.1%) showed no response to steroids. Two patients who were resistant to steroids underwent ther-apeutic surgery. The presence of gastroduodenal ulcers was significantly associated with relapse and steroid resistance.Conclusion: A high suspicion of EGE is warranted when children have nonspecific gastrointestinal symptoms and peripheral eosinophilia. Most patients improved with food restrictions or steroid treatment, although one-third of pa-tients showed a relapse or steroid resistance.

A pre-dose biomarker Ccrn4l for predicting the susceptibility of cisplatin-induced hepatotoxicity

Da-Bin Hwang,Dong-Hoon Won,Yoo-Sub Shin,Shin-Young Kim,Changuk Kim,Jun-Won Yun 한국실험동물학회 2021 한국실험동물학회 학술발표대회 논문집 Vol.2021 No.7

Cisplatin-induced liver injury is one of the limitations of its use as an anti-cancer drug. In particular, individual differences in sensitivity to drug-induced liver injury (DILI) can lead to life-threatening situations. The purpose of this study is to discover which innate genetic factors determine diversity for susceptibility to DILI. Rats undergo a liver prebiopsy and a 3-week postoperative recovery period prior to cisplatin administration. Two days after administration of cisplatin, hepatotoxicity was confirmed through serum biochemical and histopathological analysis. Based on liver-related biochemical test results (ALT, AST, γGT), rats were sub-grouped into the Susceptible (top five) or Resistant (bottom five) group for RNA sequencing using the pre-collected liver samples. As a result, pre-dose 161 genes were differentially expressed between the susceptible and resistant groups. Among them, Ccrn4l, a clock-controlled gene included in the "rhythmic process", was inherently low in gene expression before drug administration in both cisplatin- and acetaminophen-susceptible animals. In addition, in the susceptible group, the innately low expression level of Ccrn4l was maintained even after cisplatin treatment with decreased antioxidants, increased nitration, and apoptosis. When checking the correlation between Ccrn4l, antioxidant catalase, and mitochondrial RNA in the liver, it was confirmed that they showed similar mRNA expression patterns according to individual circadian variation. Surprisingly, Ccrn4l knockdown WB-F344 cells prompted cisplatin-induced mitochondrial dysfunction, apoptosis, and decreased catalase activity. In conclusion, individual innate hepatic Ccrn4l level may be a novel factor affecting cisplatin-induced hepatotoxicity susceptibility by modulating mitochondrial and antioxidant functions.

The Wnt/β-catenin signaling pathway plays a role in drug-induced liver injury by regulating cytochrome P450 2E1 expression

Shin Yoo-Sub,Hwang Da-Bin,Won Dong-Hoon,Kim Shin-Young,Kim Changuk,Park Jun Won,Jeon Young,Yun Jun-Won 한국독성학회 2023 Toxicological Research Vol.39 No.3

Drug-induced liver injury (DILI) is a major cause of acute liver failure and drug withdrawal. Cytochrome P450 (CYP) 2E1 is involved in the metabolism of several drugs, and can induce liver injury through the production of toxic metabolites and the generation of reactive oxygen species. This study aimed to elucidate the role of Wnt/β-catenin signaling in CYP2E1 regulation for drug-induced hepatotoxicity. To achieve this, mice were administered cisplatin or acetaminophen (APAP) 1 h after treatment with the CYP2E1 inhibitor dimethyl sulfoxide (DMSO), and histopathological and serum biochemical analyses were performed. APAP treatment induced hepatotoxicity, as evidenced by an increase in liver weight and serum ALT levels. Moreover, histological analysis indicated severe injury, including apoptosis, in the liver tissue of APAP-treated mice, which was confirmed by TUNEL assay. Additionally, APAP treatment suppressed the antioxidant capacity of the mice and increased the expression of the DNA damage markers γ-H2AX and p53. However, these effects of APAP on hepatotoxicity were significantly attenuated by DMSO treatment. Furthermore, the activation of Wnt/β-catenin signaling using the Wnt agonist CHIR99021 (CHIR) increased CYP2E1 expression in rat liver epithelial cells (WB-F344), whereas treatment with the Wnt/β-catenin antagonist IWP-2 inhibited nuclear β-catenin and CYP2E1 expression. Interestingly, APAP-induced cytotoxicity in WB-F344 cells was exacerbated by CHIR treatment and suppressed by IWP-2 treatment. Overall, these results showed that the Wnt/β-catenin signaling is involved in DILI through the upregulation of CYP2E1 expression by directly binding the transcription factor β-cat/TCF to the Cyp2e1 promoter, thus exacerbating DILI.

여천(與天) 이용설(李容卨)의 의료 계몽과 학술활동

신유섭 ( Yoo Sub Shin ),신재의 ( Jae Eui Shin ),유형식 ( Hyung Sik Yoo ) 연세대학교 의과대학 의사학과 의학사연구소 2014 연세의사학 Vol.17 No.1

The purpose of this study is to explore the medical enlightenment and academic activities of Dr. Yong-Sul Lee who graduated from the Severance Union Medical College in 1919. Dr. Lee was medically trained in both China and the United States, and by 1926 had developed advanced concepts about the prevention of disease, as well as about its treatment. Christian Messenger between 1927 and 1929, about his experience as a Christianity doctor working with various infectious diseases, and also surgical, pediatric and obstetric diseases. The main purpose of his articles was directed towards the medical enlightenment of the Korean people. He especially emphasized the prevention of diseases by maintaining hygiene, and he described the treatments for various diseases, and warned against the harmfulness of certain traditional medicines. His academic activities were published in those newspapers, magazines and medical journals that were concerned with infectious and orthopedic diseases. He reported on his method of treatment for amoebic abscess of the liver at the Pan Asia-Pacific Surgical Conference in 1929. He was a member of the Korean Medical Society established in 1930, and was involved in the first orthopedic surgery in Korea.

An Epidemiologic Study on Hosts and Pathogens of Urinary Tract Infection in Urban Children of Korea (2012-2017)

Yoo, Yeong Myong,Park, Byeong Sub,Lee, Shin Young,Park, Kyu Jung,Jung, Hyun Joo,Pai, Ki Soo Korean Society of Pediatric Nephrology 2019 Childhood kidney diseases Vol.23 No.1

Purpose: We aimed to determine characteristics of host, causative organisms, and antibiotic susceptibility of bacteria in pediatric patients with UTI living in metropolitan area of Korea. Methods: Retrospective investigation was done for the causative organisms of UTI in 683 pediatric cases treated at Ajou University Hospital from 2012 to 2017. Patients were classified into Escherichia coli and non-E. coli group, where E. coli group was subdivided into ESBL(+) and ESBL(-) groups based on whether the bacteria could produce extended spectrum beta-lactamase (ESBL). Antibiotic susceptibility of the causative organism was also determined. Results: A total of 683 UTIs occurred in 550 patients, of which 463 (67.8%) were first-time infection and 87 (32.2%) were recurrent ones (2-7 recurrences, 2.52 average), and 64.9% were male and 35.1% were female. The most common causative organism was E. coli (77.2%) and ESBL(+) E. coli was found in 126 cases. The susceptibility of E. coli to 3rd or 4th generation cephalosporin was relatively higher than that to ampicillin or amoxicillin/clavulanic acid. ESBL(+) E. coli showed higher resistance rate to 3rd or 4th generation cephalosporin than ESBL(-) E. coli. Conclusion: New treatment guideline should be considered due to the incidence of ESBL(+) E. coli increased up to one quarter of UTI cases.

한국 일 농촌지역 노인의 일상생활 수행능력 및 정신건강에 관한 사회정신의학적조사

신승철,김찬형,이종섭,이호영,유계준 大韓神經精神醫學會 1992 신경정신의학 Vol.31 No.6

This paper represents results of a study concerning activities of daily living(ADL) and menial health among the elderly in a Korean rural community. The parts of the Older Americans Resources and Services(OARS)-Korean version multidimentional functional assessment questionnaire were administered to a stratified second stage cluster sample of 1005 non-institulionalized elderly persons who are over 65 year old. The term impaired ADL group is used to refer to persons who have moderate to total impairment in ADL. and those who have mildly or no-impairment in ADL is referred to the normal-range group. The difference between these two groups is the level of independence in life. The percentages of impaired ADL group was significantly increased with advancing age(p<0.05). The overall incidence of impaired ADL group was 6.1%. The family was the major helper both for the impaired ADL group was (100.0%) and normal-range group (96.4 %). The ADL impairment group did significantly worse in the interviewer's assessment of mental health than the normal-range group(p<0.05). The need for doctor in impaired ADL group was greater than the normal-range group, but the number of visits to the clinic by the former was significantly fewer than the latter(p<0.05). The long-term care program is recommended for the elderly in community, especially for the impaired ADL group.

Living Related Liver Transplantation in an Infant with Neonatal Hemochromatosis

Shin Jie Choi,Jong Sub Choi,Peter Chun,Jung Kyung Yoo,Jin Soo Moon,고재성,김우선,강경훈,이남준 대한소아소화기영양학회 2016 Pediatric gastroenterology, hepatology & nutrition Vol.19 No.2

Neonatal hemochromatosis (NH) is a severe neonatal liver injury that is confirmed by extra-hepatic iron accumulation. Although a recent study described treating NH with exchange transfusions and intravenous immunoglobulin, liver transplantation should be considered for patients with severe liver failure that does not respond to other medical treatment. Herein, we report the case of a two-month-old female infant who presented with persistent ascites and hyperbilirubinemia. Her laboratory findings demonstrated severe coagulopathy, high indirect and direct bilirubin lev-els, and high ferritin levels. Abdominal magnetic resonance imaging presented low signal intensity in the liver on T2-weighted images, suggesting iron deposition. The infant was diagnosed with NH as a result of the clinical findings and after congenital infection and metabolic diseases were excluded. The infant was successfully treated with a living-donor liver transplantation. Living related liver transplantation should be considered as a treatment option for NH in infants.

유청을 이용한 영지버섯과 잎새버섯의 균사체 배양

신동화,유진영,구영조,정건섭,최신양 한국균학회 1991 韓國菌學會誌 Vol.19 No.1

For the production of mycelia of mushroom by submerged culture, the experiment was carried out. mushroom. The optimum culture broth for Ganoderma lucidum NG-L were composed of CMC 0.8%(w/v) and (NH₄)₂SO₄ 0.2%, with 10%(v/v) of inoculum size and pH 5.5 when the milk whey was used as basal medium. In case of Grifola frondosa ATCC48688, the optimum broth were composed of soluble starch 2%(w/v) and KNO₃ 0.1%(w/v), with 8%(v/v) of inoculum size and pH 5.2. Among several plant growth hormones, indole-3-acetic acid and gibberellin A₃-3acetate stimulated the mycelial growth of Ganoderma lucidum NG-L and Grifola frondosa ATCC 48688 respectively. The culture broth of these mushrooms inhibited the growth of B. subtilis and P. aeruginosa.

유선질환에서 Keratin 출현도에 관한 면역 조직화학적 관찰

신동용,박언섭,유재형,송계용 중앙대학교 의과대학 의과학연구소 1987 中央醫大誌 Vol.12 No.3

To observe the epithelial staining reaction and the distribution of cytokeratin in carcinoma and a variety of benibn breast diseases, immunohistochemical studies were carried out with paraffin embedded sections. The studies consisted of 16 cases of infiktrating duct carcinoma, 1 case of medullary carcinoma, 16 cases of fibroadenoma and 28 cases of fibrocystic diseases. Method of immunohistochemical staining was PAP(peroxidase-antiperoxidase)method. The results were as follows: 1. The weakly positive reaction with keratin were observed in 13 cases out of 16 cases of infiltrating duct carcinoma and the positivities in the epithelial of the hyperplastic ducts adjacent to carcinoma were stronger than that of carcinomas. 2. The weakly to moderately positive reactions with keratin were observed in 13 cases out of 16 cases of fibroadenoma and the positivities in the epithelial of the pericanalicular area were stronger than that of the intracanalicular area. 3. The weakly to moderately positive reactions with keratin were observed in all 28 cases of fibrocystic diseases. Adenosis and epithelial hyperplasia revealed stronger positive staining than those of fibrosis and cystic disease. 4. Therefore, reactivities of epithelial cytokeratin were stronger in the epithelium of benign condition than carcinomas and in more differentiated carcinoma than less differentiated one.

두경부암종에서 혈관내피세포성장인자 및 p53 단백발현관찰

신민수,배준우,이동기,이태진,김미경,박언섭,유재형 중앙대학교 의과대학 의과학연구소 2001 中央醫大誌 Vol.26 No.1