Fosfomycin의 항균효과 및 capillary electrophoresis에 의한 측정법에 관한 연구

윤효인,박승춘,이규승,권진욱,조준형,신광순,김무열,허강준,조명행 충남대학교 수의과대학 동물의과학연구소 1996 動物醫科學硏究誌 Vol.4 No.-

Fosfomycin, a low molecular antibiotic, is to be easily synthesized, therefore to be possibly used in veterinary sectors due to econimic reasons. In this study, we determined the antibacterial activities of fosfomycin against some domestic animal-origin pathogens, and its combined effects with some important antibacterials available. We also compared detection methods of fosfomycin from various measuring instruments. The results obtained through this experiment were as follows: 1. Antibacterial activities of fosfomycin against the pathogens used in this experiment were appeared fairly low. We found the antobacterial activities depanded on the kinds of media and the addition of some material thereins, which suggests the importance of appropriate medium so as to judge the antibacterial activity of fosfomycin. 2. Fosfomycin showed varying antibacterial effects in combination with other antibacterials, which should be taken into consideration when combining other antobacterials especially in the clinical settings. Of the combined antivacterials, fosfomycin had synergistic effects in combination with amoxicillin but antagonistic effects with oxytetracyline. 3. Conventional detection methods using HPLC, GC and spectrophotometer were not appropriate to measure fosfomycin. Capillary electrophoresis by the way of the indirect ultraviolet detection method was good enough to detect fosfomycin in the range of expected concentration in tissues, with the limit of quantitation (LOQ) of 1.25 ㎍/㎖. We recommend the eletrophoresis method could be used to detect fosfomycin, thereby being able to set the optimal dosage for animals and providing the measures in relation with the residual concentration in the meat.

토끼에서 enrofloxacin과 enrofloxacin acetate의 比較藥物動態學

윤효인,박승춘,김창식,김민규,최기섭,신광순,박종일,조준형,조명행 충남대학교 수의과대학 동물의과학연구소 1996 動物醫科學硏究誌 Vol.4 No.-

This study was designed to examine the in vitro antibacterial activities and pharmacokinetics of enrofloxacin acetate (ENFXA) and enrofloxacin (ENFX). Two enrofloxacins had wide spectrum antibacterial activities against Gram positive and negative bacteria, and mycoplasma, showing potent antibacterial activities with low MICs (0.05-3.33 ㎍/㎖ for Gram positives, 0.002 ㎍/㎖ for Gram negatives and 0.02 ㎍/㎖ for mycoplasma). In order to investigate the profiles of two formulations of enrofloxacin we studied comparative pharmacokinetics of them in rabbits. Five rabbits were given 5 ㎎/㎏ body weight of each enrofloxacin formulation intravenously (i.v.) and orally (p.o.) in a cross-over study. Pharmacokinetic parameters of both formulations were calculated by the use of PCNONLIN, a computer program. Their bioavailibility in rabbits, the means of the area under the curve (AUC). After oral administration of each formulation in rabbits, the mean elimination half-lives (t_1/2,ke) were 3.25h (ENFX) and 4.32 h (ENFXA), and mean AUC 4.27 ㎍·h/㎖ (ENFX) and 6.21 ㎍·h/㎖ (ENFXA). Both enrofloxacin formulations seemed to have good tissue distribution and penetration as indicated by large volume of distribution: 4.76 1/㎏ for ENFX and 7.23 1/㎏ for ENFXA. With the results obtained in this study, ENFXA could be used in place of ENFX in rabbits.

관동맥 우회로 이식술후 우회 혈관의 개존 여부 평가에 있어 전자선 단층 촬영을 이용한 3차원 혈관조영술의 유용성

하종원,조승연,심원흠,정남식,이향미,최규옥,장양수,유경종,강면식 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.5

돼지에서 정맥, 근육 그리고 경구 투여시의 enrofloxacin의 약물동태학

윤효인,김무열,박승춘,조준형,박병권,이내경,노상석,장범수,신광순,조명행 충남대학교 수의과대학 동물의과학연구소 1996 動物醫科學硏究誌 Vol.4 No.-

In order to characterize pharmacokinetic profiles according to route of a new enrofloxacin salt form (Enrotil®), it was given to 4 healthy pigs via oral (p.o.), intramuscular (i.m.) or intravenous (i.v.) administrations at a dose rate of 5 ㎎/㎏ body weight. Enrofloxacin (ENFX) in serum was detected by bioassay using E. coli BE1186 as a test organism. The biological elimination half-lives (t_1/2(β)) of ENFX were 6.76±0.99 h (i.v.), 7.16±2.30 h (i.m.) and 11.45±3.90 h (p.o.), Volume of distribution (Vd) of enrofloxacin was 2.20±0.31 L/㎏ (i.v.), 2.52±0.60 L/㎏ (i.m.) and 1.88±0.33 L/㎏ (i.m.). Mean residence time (MRT) was 8.77±1.26 h after i.v. injection and the maximal concentration time (Tmax) following p.o. and i.m. administration was 0.76±0.09 h and 0.60±0.12 h, indicating a rapid absorption from these routes. Bioavailibility (F) was calculated as 64.1% for p.o. administration and 59.71% for i.m. injections. In summary, the newly formulated enrofloxacin salt form has shown a high water solubility, rapid absorption and large tissue distribution, suggesting a potential antibacterials for oral application on a large scale in veterinary sectors.

변이형 협심증 환자의 진단에 있어 Iodine-123Metaiodobenzylguanidine 심근 스캔의 유용성

하종원,이종두,장양수,정남식,권준,임세중,이영준,심원흠,조승연,김성순 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.2

육제품 중 아질산염 잔류량과 몇 가지 농산물의 질산염 함량

하정욱,이승철,윤이란 慶南大學校 附設 工業技術硏究所 1998 硏究論文集 Vol.16 No.-

식육 햄, 식육 소시지 및 어육 소시지 등의 시료를 각각 15개씩 취하여 아질산염 잔류량을 정량하고, 또한 구기자, 오미자, 결명자, 시금치, 오렌지, 피망, 고추, 마늘 및 레몬 등의 몇가지 농산물을 구입하여 질산염의 함량을 측정한 결과는 다음과 같았다. 즉, 육제품의 경우 아질산염 잔류량은 식육 햄 12.8∼32.85 ppm, 식육 소시지 1.12∼14.71 ppm 및 어육 소시지 0.02∼0.45 ppm으로 식육 제품은 큰 변동치를 보였으나 어육 제품은 상당히 낮은 수준의 잔유량을 나타내었다. 한편 어육 소시지 15개 시료 중 8개 시료에서는 아질산염이 검출되지 않았다. 농산물 시료 중의 질산염 함량은 구기자 10,347 ppm(건물기준), 피망 7,236 ppm, 결명자 5,820 ppm(건물기준), 오렌지 4,152 ppm으로 유럽의 기준치 (1500∼3500 ppm)보다 많았으며, 나머지는 오미자 2,910 ppm, 시금치 2,078 ppm, 마늘 1,061 ppm, 고추 575 ppm, 레몬 170 ppm 순이었다. The residues of nitrite in meat products such as meat ham, meat sausage and fish sausages were determined with 15 samples, respectively. And the nitrate content of various agricultural products such gugija (Lycium chinese Mill.), omija(Maximowiczia chinensis Repr.), gyeolmyeongja (Cassia tora L.), spinach, orange, sweet pepper, red pepper, garlic bulb and lemon. The residues of nitrite in meat ham, meat sausage and fish sausage were 12.85∼32.85 ppm, 1.12∼14.71 ppm and 0.02∼0.4 ppm, respectively. The average residual content of nitrite in fish sausage was 0.17±0.13 ppm and was not detected from 7 samples of fish sausage among 15. The respective nitrate content of gugija, gyeolmyeongja and sweet pepper were 10.347 ppm (dry basis ), 5,820 ppm and 7.236 ppm (dry basis) and other results showed 4,152 ppm for orange, 2,910 ppm for omija, 2.78 ppm for spinach, 1,061 ppm for garlic bulb, 575 ppm for red pepper and 170 for lemon.

시금치 및 마늘의 아질산염 소거작용 및 전자공여작용

하정욱,이승철,윤이란 경남대학교 공업기술연구소 2000 硏究論文集 Vol.18 No.-

주요 야채 및 향신료로 이용되고 있는 시금치와 마늘로부터 물 추출물과 메탄을 추출물을 조제한 다음, phenol 화합물의 함량, 전자공여작용 및 아질산염 소거작용 등을 측정하고 검토하였다. 시금치와 미늘의 methanol 추출물이 물 추출물보다는 다소 높은 전자공여 작용을 나타내었으며, 방향족 화합물의 함량은 시금치의 methanol 추출물과 마늘의 물 추출물이 다른 두 종류의 추출물 보다는 비교적 높은 전자공여작용을 보였다. 그리고 물 추출물과 methanol 추출물 중에서 pH1.2에서 마늘의 물 추출물이 98.62%로 가장 두드러진 아질산염 소거율을 나타내었으며, 이 외에는 아질산염 소거작용면에서 거의 전반적으로 methanol 추출물이 물 추출물보다는 훨씬 효과적이었다. This study was conducted to investigate the electron donating ability(EDA) and nitrite scavenging ability of water extracts and methanol extracts from spinach and garlic-bulb. Methanol extracts from two samples showed comparatively higher EDA, and the content of phenolic substances of methanol extract from spinach and water extract from garlic-bulb was more than another extracts. The nitrite scavenging ability of water extracts from garlic-bulb showed the highest ratio of 98.62% among those extracts at pH1.2 and the decreasing tendency was clearly pH-dependent. Generally methanol extracts from two samples showed higher effective nitrite scavenging abilities than water extracts.

n-CdS_(0.46)Se_(0.54)/p-Cu_92-x)S_(0.46)Se_(0.54) 이종접합 태양전지의 제작과 그 특성에 관한 연구

유상하,최승평,이상열,홍광준,서상석,김혜숙,전승룡,윤은희,문종대,신영진,정태수,신현길,김택성,유기수 全北大學校 基礎科學硏究所 1994 基礎科學 Vol.16 No.-

승화방법에 의해 CdS_0.46Se_0.54 단결정을 성장하여 결정구조를 조사하고, Van der Pauw 방법으로 Hall effect를 측정하여 carrier density의 온도 의존성과 mobility의 온도 의존성을 조사하였다. 성장된 CdS_0.46Se_0.54 단결정을 치환반응하여 n-CdS_0.46Se_0.54/p-Cu_2-xS_0.46Se_0.54 이종접합 태양전지를 제작하였다. Spectral response, 전류-전압특성 및 전력변환 효율을 조사하여 그 결과로부터 개방전압은 0.48V, 단락 전류 밀도는 21mA/㎠, fill factor와 전력변환효율은 각각 0.75와 9.5%를 얻었다. CdS_0.46Se_0.54 single crystal was grown by a sublimation method. The crystal structure and the temperature dependence of carrier density and mobility of CdS_0.46Se_0.54 single crystal were studied. Heterojunction solar cells on n-CdS_0.46Se_0.54/p-Cu_2-xS_0.46Se_0.54 were fabricated by the substitution reaction. The spectral response, the J-U characteristics and the conversion efficiency of the n-CdS_0.46Se_0.54/p-Cu_2-xS_0.46Se_0.54 heterojunction solar cells were studied. The open-cricuit voltage, short-circuit density, fill factor and conversion efficiency of n-CdS_0.46Se_0.54/p-Cu_2-xS_0.46Se_0.54 heterojunction solar cells under 80mW/㎠ illumination were found to be 0.48V, 21mA/㎠, 0.75 and 9.5%, respectively.

랫드에서 신우황청심원의 급성 및 아급성독성시험

오승민,남혜윤,김준수,연제덕,신대희,이진영,박대규,조명행,정규혁 성균관대학교 약학연구소 1998 成均藥硏論文集 Vol.10 No.1

The acute and subacute toxicity of New Woohwangchungsimwon(NWCH) which was used l-muscone as substitutive material of musk were investigated in S.D. rats. In intraperitoneal acute toxicity test, rats(Sprague-Dawley, SPF) were injected intraperitoneally with dosages of 0, 540, 750, 1,070, 1,500 and 3,000 ㎎/㎏. Body weights were significantly decreased at 540 ㎎/㎏ dose group in both sexes and abnormal autopsy findings were founded in both sexes at all treated groups. Intraperitoneal LD_50 of NWCH was 812.3 ㎎/㎏ in male and 872.3 ㎎/㎏ in female rats. In the subacute toxicity study, NWCH was administrated orally to both sexes of rats for 4 weeks as several doses(0, 320, 800 and 2,000 ㎎/㎏). There were neither dead animals nor significant changes of body weights during the experimental period. In addition, no differences were found between control and treated groups in clinical signs, urinalysis, hematology, serum biochemical analysis, and other findings. Above data strongly suggest that no observed adverse effect level of NWCH might be over 2,000 ㎎/㎏/day in this study.

국내 검정콩 육성품종의 종자특성

주용하,박재훈,윤승길,김영호,김성민,정길웅 한국국제농업개발학회 2002 韓國國際農業開發學會誌 Vol.14 No.4

1. 수분흡수율이 가장 높은 시간은 다원콩(침지 후 4시간)을 제외한 모든 품종이 침지후 2시간이었으며, 둔화되기 시작한 시간은 침지후 약 10시간이었고, 평형에 도달한 시간은 침지후 약 16시간이었다. 2. 수분흡수율은 품종간에 차이가 있었는데 가장 높은 수분흡수율을 보인 품종은 검정콩2호이었으며, 가장 낮은 품종은 다원콩이었다. 3. 발아율의 범위는 28이었으며, 평균발아율은 93.9%이었고, 가장 높은 품종은 청자콩과 흑청콩이었다. 4. 발아세의 범위는 52이었으며, 평균발아세는 81.9%이었고, 가장 높은 품종은 흑청콩이었다. 5. 평균발아일수와 T_50의 범위는 모두 2이었으며, 평균은 각각 2.6과 2.3이었고, 품종간에는 선흑콩이 가장 길었다. 6. 발아균일도의 범위는 3.1이었으며, 평균은 1.3이었고, 높은 그룹(흑청콩·청자콩·검정콩2호·다원콩·검정올콩)과 낮은 그룹(검정콩1호·일품검정콩·선흑콩)으로 분류되었다. 7. 알칼리붕괴도는 공시품종의 평균이 4.65이었으며 등급은 4∼5등급을 나타내었다. 붕괴도가 가장 높고 우수한 품종은 검정콩1호였으며 가장 낮은 품종은 선흑콩이었다. The water absorption rate after soaking of seeds was the highest at 2 hours in all varieties except Dawonkong(4 hours after soaking), and became slowed down at 10 hours, and reached moisture equilibrium in stopping almost at 16 hours. The water absorption rate was different among varieties, the highest variety was Geomjeongkong #2 whereas the lowest variety was Dawonkong. The range of germination percentage was 28, and average value 93.9%, and the highest varieties were Cheongjakong and Heukcheongkong. The range of germination speed was 52. average value 81.9%, and the highest variety was Heukcheongkong. The range of average days to germination and T_50 were all two days and these average was 2.6 and 2.3, respectively. Seonheukkong among varieties was very long in average days to germination and T_50. The range of germination uniformity was 3.1 and average was 1.3 and divide into two groups such as high group(Heukcheongkong, Cheongjakong, Gemjeongkong #2, Dawonkong, Geomjeongolkong) and low group(Geomjeongkong #1, llpumgeomjeongkong, Seonheukkong). Varietal mean of alkali digestibility value was 4.65 belong to 4∼5 class, the highest variety was Geomjeongkong #1 whereas Seonheukkong was very low among varieties.