육제품 중 아질산염 잔류량과 몇 가지 농산물의 질산염 함량

하정욱,이승철,윤이란 慶南大學校 附設 工業技術硏究所 1998 硏究論文集 Vol.16 No.-

식육 햄, 식육 소시지 및 어육 소시지 등의 시료를 각각 15개씩 취하여 아질산염 잔류량을 정량하고, 또한 구기자, 오미자, 결명자, 시금치, 오렌지, 피망, 고추, 마늘 및 레몬 등의 몇가지 농산물을 구입하여 질산염의 함량을 측정한 결과는 다음과 같았다. 즉, 육제품의 경우 아질산염 잔류량은 식육 햄 12.8∼32.85 ppm, 식육 소시지 1.12∼14.71 ppm 및 어육 소시지 0.02∼0.45 ppm으로 식육 제품은 큰 변동치를 보였으나 어육 제품은 상당히 낮은 수준의 잔유량을 나타내었다. 한편 어육 소시지 15개 시료 중 8개 시료에서는 아질산염이 검출되지 않았다. 농산물 시료 중의 질산염 함량은 구기자 10,347 ppm(건물기준), 피망 7,236 ppm, 결명자 5,820 ppm(건물기준), 오렌지 4,152 ppm으로 유럽의 기준치 (1500∼3500 ppm)보다 많았으며, 나머지는 오미자 2,910 ppm, 시금치 2,078 ppm, 마늘 1,061 ppm, 고추 575 ppm, 레몬 170 ppm 순이었다. The residues of nitrite in meat products such as meat ham, meat sausage and fish sausages were determined with 15 samples, respectively. And the nitrate content of various agricultural products such gugija (Lycium chinese Mill.), omija(Maximowiczia chinensis Repr.), gyeolmyeongja (Cassia tora L.), spinach, orange, sweet pepper, red pepper, garlic bulb and lemon. The residues of nitrite in meat ham, meat sausage and fish sausage were 12.85∼32.85 ppm, 1.12∼14.71 ppm and 0.02∼0.4 ppm, respectively. The average residual content of nitrite in fish sausage was 0.17±0.13 ppm and was not detected from 7 samples of fish sausage among 15. The respective nitrate content of gugija, gyeolmyeongja and sweet pepper were 10.347 ppm (dry basis ), 5,820 ppm and 7.236 ppm (dry basis) and other results showed 4,152 ppm for orange, 2,910 ppm for omija, 2.78 ppm for spinach, 1,061 ppm for garlic bulb, 575 ppm for red pepper and 170 for lemon.

Micro-HPLC를 이용한 조제분유 중 비타민 A,E 동시분석법 개발

윤이란 ( I Ran Yun ),최유정 ( You Jeong Choi ),이민권 ( Min Kwon Lee ),정명호 ( Byeong Hun Kim ),김병훈 한국가축위생학회 2006 韓國家畜衛生學會誌 Vol.29 No.3

Semi-micro-HPLC using a column-switching technique was developed for simul-taneous determination of vitamin A and E contents in infant formula. Vitamin A and E were extracted by PDA-HPLC with reversed phase column using organic solvent and their contents in Certified Reference Material(CRM) and infant formula were deter-mined and compared with hydrolysis method and rapid extraction. Developed method has many advantages of simple and rapid sample preparation and simultaneous deter-mination of vitamin A and E by micro-HPLC using reversed phase column.

갈근 및 녹차추출물의 아질산염 소거작용 및 간독성 보호효과

윤이란 ( I Ran Yun ),최유정 ( You Jeong Choi ),허정호 ( Jung Ho Heo ),최철웅 ( Chul Yung Choi ),성태종 ( Tae Jong Seoung ),김윤근 ( Yun Geun Kim ),김종수 ( Jong Shu Kim ) 한국가축위생학회 2010 韓國家畜衛生學會誌 Vol.33 No.3

This study was designed to evaluated the nitrite scavenging activity and protective effect of the Puerariae Radix and green tea extract on lead acetate and cadmium-induced liver damage in mice. The quantitative analytical method for major antioxidants, isoflavones, puerarin, catechine and caffeine in galgun (Puerariae Radix) and green tera extract were established by HPLC. Contents of isoflavones, such as daidzin, genistin, daidzein and genistein were 4.23g/100g, 0.13g/100g, 0.07g/100g, and 0.03g/100g, and puerarin contents was 8.99g/100g, respectively. The total catechins and caffeine contents of green tea extract were 49.24g/ 100g and 6.53g/100g. The nitrite scavenging ability of galgun extract (pH 1.2, 100mg/ml) was 98.07% and it was higher than those of other extracts. It was the highest at the pH 1.2 and more than 64% in 25~ 100mg green tea extract, and was dependents on pH and concentration of the samples. The hepatoprotective effects of an aqueous extract from the root of galgun and standard puerarin were evaluated against lead acetate and cadmium-induced liver damage in mice. Galgun extract and standard at a dose of 100mg/kg and 10mg/kg, 50mg/kg were administered orally once daily for successive 5 days and then a lead acetate and cadmium were orally at 3 hrs after the every day administration of galgun. The substantially elevated serum enzymatic activities of alanine and aspartate aminotransferase were due to lead acetate and cadmium treatment was dose dependently restored to the near normal level. In addition, galgun extract also significantly prevented the elevation of hepatic malon-dialdehyde formation in the liver of lead acetate and cadmium intoxicated mice in a dose-dependent manner. The results of this study clearly indicated that green tea and galgun extracts had nitrite scavenging ability and galgun extract had potent hepato-protective effects against lead acetate and cadmium-induced hepatic damage in mice and standard puerarin was also showed similar to the results of the galgun extracts.

구기자, 오미자 및 결명자의 아질산염 소거작용

하정욱,황용일,윤이란 慶南大學校 附設 工業技術硏究所 1999 硏究論文集 Vol.17 No.-

기호음료의 재료인 구기자, 오미자, 및 결명자 등으로부터 물 추출물과 메탄올 추출물을 조제한 다음, phenol 화합물의 함량, 전자공여작용 및 아질산염 소거작용 등을 관찰하고, 이들 요인 간의 상관관계를 검토하였다. 추출물 중 오미자와 결명자의 메탄올 추출물이 비교적 높은 전자공여작용을 나타내었고, 방향족 화합물의 함량은 결명자>구기자>오미자의 순이었다. 물추출물의 아질산염 소거작용은 pH 1.2에서 구기자가 85.61%로 가장 현저하였고, 소거율은 구기자>오미자>결명자의 순이었으며, pH의존적으로 감소하였다. 메탄올 추출물의 아질산염 소거작용은 pH 1.2에서 구기자 96.03%. 결명자 89.38%. 및 오미자 87.23%로 매우 높은 소거율을 보였으며, pH 증가시 급격하게 감소하는 경향을 나타내었다. 그리고. 아질산염 소거작용과의 상관관계면에서 전자공여작용은 비교적 낮은 상관계수를 나타내었고, 방향족 화합물은 낮은 pH에서는 비교적 높은 값을 보였으나, pH 6.0에서는 상관관계를 거의 볼 수 없었다. 그리고 전반적으로 메탄올 추출물이 물 추출물에 비해 높은 아질산염 소거율을 유지하였다. This study was conducted to investigate the content of phenolic compounds, electron donating ability(EDA) and nitrite scavenging ability of water extracts and methanol extracts from Lycium chinese Mill Shizanda chinensis Baillon, and Cassia tora L. Methanol extracts from Shizandra chinensis Baillon showed comparatively higher EDA, and the content of phenolic compounds was in the sequence of Cassia tora L.>Lycium chinense Mill>Schizandra chinensis Baillon. The nitrite scavenging ability of water extracts from Lycium chinense Mill showed higher ratio of 85.61% than Shizandra chinensis Baillon and Cassia tora L. at pH 1.2, and decreased remarkably in the higher range of pH. The nitrite scavenging ratios of methanol extracts were 96.03% of Lycium chinense Mill, 89.30% of Cassia tora L. and 87.23% of Shizandra chinensis Baillon and the decreasing tendency was clearly pH-dependent. Generally methanol extracts showed higher coefficients than water extracts and the content of phenolic compounds had higher coefficients at lower pH range against nitrite scavenging ability.

시금치 및 마늘의 아질산염 소거작용 및 전자공여작용

하정욱,이승철,윤이란 경남대학교 공업기술연구소 2000 硏究論文集 Vol.18 No.-

주요 야채 및 향신료로 이용되고 있는 시금치와 마늘로부터 물 추출물과 메탄을 추출물을 조제한 다음, phenol 화합물의 함량, 전자공여작용 및 아질산염 소거작용 등을 측정하고 검토하였다. 시금치와 미늘의 methanol 추출물이 물 추출물보다는 다소 높은 전자공여 작용을 나타내었으며, 방향족 화합물의 함량은 시금치의 methanol 추출물과 마늘의 물 추출물이 다른 두 종류의 추출물 보다는 비교적 높은 전자공여작용을 보였다. 그리고 물 추출물과 methanol 추출물 중에서 pH1.2에서 마늘의 물 추출물이 98.62%로 가장 두드러진 아질산염 소거율을 나타내었으며, 이 외에는 아질산염 소거작용면에서 거의 전반적으로 methanol 추출물이 물 추출물보다는 훨씬 효과적이었다. This study was conducted to investigate the electron donating ability(EDA) and nitrite scavenging ability of water extracts and methanol extracts from spinach and garlic-bulb. Methanol extracts from two samples showed comparatively higher EDA, and the content of phenolic substances of methanol extract from spinach and water extract from garlic-bulb was more than another extracts. The nitrite scavenging ability of water extracts from garlic-bulb showed the highest ratio of 98.62% among those extracts at pH1.2 and the decreasing tendency was clearly pH-dependent. Generally methanol extracts from two samples showed higher effective nitrite scavenging abilities than water extracts.

녹차 추출물의 첨가가 저장 중 유화형 소시지의 품질 특성 및 아질산염 감소에 미치는 영향

양한술,정진연,이정일,윤이란,주선태,박구부 한국축산식품학회 2006 한국축산식품학회지 Vol.26 No.4

LC/MS/MS를 이용한 식용란 중 fluoroquinolone계 항균물질의 분석법에 관한 연구

최유정 ( You Jeong Choi ),윤이란 ( I Ran Yun ),남상윤 ( Sang Yun Nam ),박영호 ( Young Ho Park ),김병훈 ( Byeong Hun Kim ),손성기 ( Seong Gi Son ) 한국가축위생학회 2007 韓國家畜衛生學會誌 Vol.30 No.1

An atmospheric pressure chemical ionization(APcI) LC/MS/MS method was developed for the simultaneous analysis of fluoroquinolones(norfloxacin, ciprofloxacin, enrofloxacin, danofloxacin) residues in eggs. The spiked and blank samples were extracted from whole eggs using 50mM phosphate buffer(pH7.4). The extract was cleaned up by passage though Oasis(R) MAX extraction cartridge for solid-phase extraction followed by elution with 4% formic acid in methanol. The extract of sample was separated on a Waters AtlantisTM dC18 reversed-phase column(4.6×150㎜, 5㎛) and analyzed by APcI positive mode mass spectrometry. The mobile phase consists of aqueous 0.2% nonafluoropentanoic acid(NFPA) and methanol. Multiple reaction monitoring(MRM) using the precursor to product ion combinations of m/z 320 → 302, 332 → 314, 360 → 342 and m/z 358 → 340 were used to quantify norfloxacin(NOR), ciprofloxacin(CIP), enrofloxacin(ENR) and danofloxacin(DAN), respectively. The limits of quantification(LOQ) were 7.8ppb for NOR, 8.5ppb for CIP, 8.9ppb for ENR, and 4.8ppb for DAN. Average recoveries of fortified sample at levels of 0.025 to 0.1ppm were estimated 71.29% for NOR, 75.27% for CIP, 85.51% for ENR and 81.22% for DAN. These results could be applied for the confirmation and quantification in eggs.

돼지 분변 및 도체에서 분리한 대장균, 장구균의 항생제 내성율 조사

정귀옥 ( Kwi Ok Jeong ),허정호 ( Jung Ho Heo ),이종민 ( Jong Min Lee ),윤이란 ( I Ran Yun ),최유정 ( You Jeong Choi ),김종수 ( Jong Shu Kim ) 한국가축위생학회 2010 韓國家畜衛生學會誌 Vol.33 No.3

The present study was conducted to investigate isolation and antimicrobial resistance ratio of E. coli, E. faecium and E. faecalis from feces(150 samples) and carcasses (150 samples) on slaughtered pigs from 6 slaughterhouse of 13 cities in the Gyeongnam during the period from January 2009 to December 2009. Isolation ratio of E. coli from feces and carcasses were 98 (65.3%) and 110 (73.3%), respectively, and simultaneously, E. faecalis and E. faecium from feces and carcasses were isolated 21 (14%), 52(34.7%) and 18 (12%), 14 (9.3%), respectively. All E. coli isolated from feces and carcasses except cefepime (0%) and ceftiofur (0%) were exhibited 2.4~83.6% of resistance to teteracycline (83.6%), ampicillin (68.2%), streptomycin (60%), chloram-phenicol (53.8%) and cephalothin (2.4%). All E. faecalis isolated from feces and carcasses except penicillin(0%) and vancomycin (0%) were exhibited 2.7~80.8% of resistance to teteracycline (80.8%), quinupristin/dalfopristin (78%), erythromycin (56.1%), streptomycin (43.8%) and bacitracin (2.7%). All E. faecium isolated from feces and carcasses except gentamicin (0%), vancomycin (0%), florfenicol (0%), linezloid (0%) and bacitracin (0%) were exhibited 3.1~53.1% of resistance to rifampin (53.1%), erythromycin and tetracycline (25%), penicillin (15.6%), ciprofloxacin (9.3%), and streptomycin, chloramphenicol, and quinupristin/dalfopristin (3.1%). According to the heard size, resistance ratio of E. coli strains isolated from feces and carcasses in slaughtered pigs-breeding farms over 1,500 heard to tetracycline, ampicillin, streptomycin and chloramphenicol showed higher resistance ratio (1.0 ~16.8%) than those of farms-breeding under 1,500 heard. From the our results, we suggest that a few of antimicrobials were used in the Gyeongnam than the other cities.