사매가 Ovalbumin감작으로 유발된 천식 생쥐 모델에서 기관지염증 억제에 미치는 영향

임중근,노성수,길기정,이영철,서영배 대한본초학회 2005 大韓本草學會誌 Vol.20 No.1

Asthma is a chronic inflammatory disease of the airways, characterized by airway eosinophilia, goblet cell hyperplasia with mucus hypersecretion, and hyper-responsiveness to both inhaled allergens and nonspecific stimuli. Eosinophil are important in the pathogenesis of asthma, with release of inflammatory mediators such as histamine, leukotrienes, cytokines, and IgE after exposure to allergen. Objectives : This research investigates the effect of the extract of Duchesnea chrysanta (EDC) on OVA-indued asthma murine model. Methods : After i.p of C57BL/6 mice, OVA sensitization (day 0) were challenged intratracheally with OVA on days 8 and 21. EDC administered weeks 8, blocked the airway inflammatory response to OVA assessed 24 h after the last OVA challenge on day 22. Results : The CC chemokine receptor 3 (CCR3) is expressed by eosinophils, mast cells, and Th2 cells. The role of CCR3 in a murine model of allergic skin inflammation induced by repeated epicutaneous sensitization with ovalbumin (OVA), and characterized by eosinophil skin infiltration, local expression of Th2 cytokines, and airway hyperresponsiveness (AHR) to inhaled antigen. The administration of EDC during OVA inhalation significantly reduced CCR3 plays an essential role in eosinophil recruitment to the skin and the lung and in the development of AHR.. EDC extract reduced total lung cells and eosinophils in bronchoalveolar lavage fluid, airway tissue eosinophilia, goblet cell hyperplasia, mucus secretion, and peribronchial edema. The inhibitory effect on airway eosinophilia and AHR was associated with reduced levels of IL-4, IL-5, and IL-13 in the bronchoalveolar lavage fluid as well as reduced serum levels of OVA-IgE. We show that OVA inhalation following OVA immunization increased airway responsiveness to induced IL-113, IL-4 and IL-5 mRNA expression in the lung tissue. The administration of EDC during OVA inhalation significantly reduced this increased airway hyperresponsiveness and also inhibited airway inflammation. Conclusions : These results support EDC as a therapeutic target in asthma and indicate that selective EDC inhibitors can reduce allergic airway inflammation.

한국인 직무 스트레스 측정도구의 개발 및 표준화

장세진,고상백,강동묵,김성아,강명근,이철갑,정진주,조정진,손미아,채창호,김정원,김정일,김형수,노상철,박재범,우종민,김수영,김정연,하미나,박정선,이경용,김형렬,공정옥,김인아,김정수,박준호,현숙정,손동국 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Background and Purposes: Over the past three decades, numerous studies performed in Korea have reported that job stress is a determinant risk factor for chronic diseases and work disability. Every society has its own culture and occupational climate particular to their organizations, and hence experiences different occupational stress. An occupational stress measurement tool therefore needs to be developed to estimate it objectively. The purpose of this study is to develop and standardize the Korean Occupational Stress Scale (KOSS) which is considered to be unique and specific occupational stressors in Korean employees. Subjects and Methods: Data were obtained from the National Study for Development and Standardization of Occupational Stress (NSDSOS Project: 2002-2004). A total of 12,631 employees from a nationwide sample proportional to the Korean Standard Industrial Classification and the Korean Standard Occupational Classification were administered. The KOSS was developed for 2 years (2002-2004). In the first year, we collected 255 items from the most popular job stress measurement tools such as JCQ, ERI, NIOSH and OSI, and 44 items derived from the a qualitative study (depth interview). Forty-three items of KOSS, in the second year, were retained for use in the final version of the KOSS by using Delphi and factor analysis. Items were scored using conventional 1-2-3-4 Likert scores for the response categories. Results: We developed eight subscales by using factor analysis and validation process: physical environment (3 items), job demand (8 items), insufficient job control (5 items), interpersonal conflict (4 items), job insecurity (6 items), organizational system (7 items), lack of reward (6 items), and occupational climate (4 items). Together they explained 50.0% of total variance. Internal consistency alpha scores were ranged from 0.51 to 0.82. Twenty-four items of the short form of the KOSS (KOSS-SF) were also developed to estimate job stress in the work setting. Because the levels of the subscales of occupational stress were gender dependent, gender-specific standard norms for both the 43-item full version and the 24-item short form using a quartile for the subscales of KOSS were presented. Conclusion: The results of this study suggest that KOSS might be an appropriate measurement scale to estimate occupational stress of Korean employees. Further and more detailed study needs to be conducted to improve the validity of this scale.

無酸素性 運動能力과 生理學的 變因의 關聯性

김기진,윤성원,김학열,정정진,우도영,노성규,손태열,안의수 江原大學校附設 體育科學硏究所 1991 江原大學校附設體育科學硏究所論文集 Vol.- No.16

Wingate test 및 Supramaximal exercise를 이용한 무산소성 운동능력의 측정시 나타난 변인의 관련성을 살펴본 본 연구를 요약하면 다음과 같다. Wingate test시 혈중젖산농도 최고치는 9.04±0.62mM로서 Supramaximal exercise시 보다 유의하게 높았으며, 혈장 LDH 및 CPK활성도는 두가지 운동부하후 모두 유의한 변화는 나타나지 않았다. 근전도상의 적분치, 중간파형빈도 및 평균파형빈도도 유의한 변화는 없었다. 등속성 수축시의 최대토오크는 양쪽 다리 모두에서 초당 60도의 각속도에서가장 높았다. Wingate test시의 최대파워는 등속성 수축시 최대토오크 및 Wingate test시 중간파형빈도의 직접적인 영향을 받는 것으로 나타났으며, 평균파워는 Wingate test시의 적분치 변화, 듣는 쪽 슬관절의 등속성 수축시최대토오크 및 Supramaximal exercise시 혈장 LDH 활성도의 직접적인 영향을 받는 것으로 나타났다. 이상의 결과에서 무산소성 운동능력과 관련지어 그 기전을 살피고자 할 때 체내 화학적 변화, 전기생리적 변화 및 발현능력의 복합성을 고려해야 하며 최대누적 산소결핍량의 활용가능성은 측정강도 결정방법을 중심으로한 계속적인 연구가 요구된다. The present study examined the relationship of variables related to anaerobic capacity with Wingate test and supramaximal exercise. Peak value of blood lactate concentration with Wingate test showeda significantly higher than suprammaximal exercise, and plasma LDH & median power frequency and mean power frequency were not any difference between two tests. Peak torque of isokinetic contractionh on knee joint showed a highest value in 60 degrees of angular velocity among a various angular velocity. Peak power of Wingate test was related to peak torque of isokinetic contraction and median power frequency of EMG with Wingate test, and mean power of Wingate test was related to integrated EMG with Wingate test, peak torque with isokinetic contraction of dominant knee joint and plasma LDH activity with supramaximal exercise. In this result, when it analyzed the mechanism of anaerobic capacity, ti was required the consideration of complexity according to biochemical alteration, electrophysiological alteration and revelation of performance, Appicapability of maximal accumulated O₂deficit was demaned a continuous study with the focus of exercise intensity.

Development of Medifood Material by the Application of Korean Medicine Theory

Seong-Soo Roh 한국식품영양과학회 2019 한국식품영양과학회 학술대회발표집 Vol.2019 No.10

Toosendan Fructus ameliorates the pancreatic damage through the anti-inflammatory activity in non-obese diabetic mice : Erratum

( Seong-soo Roh ),( Yong-ung Kim ) 대한본초학회 2016 大韓本草學會誌 Vol.31 No.1

Toosendan Fructus ameliorates the pancreatic damage through the anti-inflammatory activity in non-obese diabetic mice

Roh, Seong-Soo,Kim, Yong-Ung The Korea Association of Herbology 2015 大韓本草學會誌 Vol.30 No.2

Objectives : The present study was conducted to examine whether Toosendan Fructus has an ameliorative effect on diabetes-induced alterations such as oxidative stress and inflammation in the pancreas of non-obese diabetic (NOD) mice, a model of human type I diabetes. Methods : Extracts of Toosendan Fructus (ETF) were administered to NOD mice at three doses (50 mg/kg, 100 mg/kg and 200 mg/kg). Mice at 18 weeks of age were measured glucose tolerance using intraperitoneal glucose tolerance test. After 28 weeks of ETF treatment, glucose, total cholesterol (TC), triglyceride (TG), and proinflammatory cytokines in serum, western blot analyses and a histopathological examination in pancreas tissue, and on the onset of diabetes were investigated. Results : The results showed that levels of glucose, glucose tolerance, TC, TG, interferon-${\gamma}$, interleukin (IL)-1 ${\beta}$, IL-6, and IL-12 in serum were down-regulated, while IL-4, IL-10, SOD, and catalase significantly increased. In addition, ETF improved protein expression of proinflammatory mediaters (such as cyclooxygenase-2, and inducible nitric oxide synthase) and a proapoptotic protein (caspase-3) in the pancreatic tissue. Also, in the groups treated with ETF (100 mg/kg or 200 mg/kg), insulitis and infiltration of granulocytes were alleviated. Conclusions : Based on these results, the anti-diabetic effect of ETF may be due to its anti-inflammatory and antioxidant effect. Our findings support the therapeutic evidence for Toosendan Fructus ameliorating the development of diabetic pancreatic damage via regulating inflammation and apoptosis. Our future studies will be focused on the search for active compounds in these extracts.

Status and Change in Chemical Properties of Polytunnel Soil in Korea from 2000 to 2012

Seong Soo Kang,Ahn Sung Roh,Seung Chul Choi,Young Sang Kim,Hyun Ju Kim,Moon Tae Choi,Byoung Gu Ahn,Hee Kwon Kim,Sang Jo Park,Young Han Lee,Sang Ho Yang,Jong Soo Ryu,Yeon Gyu Sohn,Myeong Sook Kim,Myung 한국토양비료학회 2013 한국토양비료학회지 Vol.46 No.6

Chemical properties of agricultural soils in Korea have been investigated at four-year interval in order of paddy, polytunnel, upland, and orchard soils since 1999; polytunnel soils were investigated over the whole country in 2000, 2004, 2008, and 2012. Polytunnel soils were taken from the surface (0-15 cm) and subsurface (15-30 cm) at 2,651, 1,274, 1,374 and 1,374 sites in all provinces of South Korea. One hundred sampling sites located in more than 400 m altitude were additionally investigated in 2008 and 2012. Average of soil chemical properties in 2012 except Jeju province were 6.6 for pH, 3.2 dS m<SUP>-1</SUP> for EC, 37 g kg<SUP>-1</SUP> for organic matter (OM), 1,049 mg kg<SUP>-1</SUP> for available (Avail.) phosphate, 1.58 cmolc kg<SUP>-1</SUP> for exchangeable (Exch.) K, 10.6 cmolc kg<SUP>-1</SUP> for Exch. Ca, and 3.3 cmolc kg<SUP>-1</SUP> for Exch. Mg. Except pH, averages of all chemical properties exceeded the upper limit of optimal range. The median values except pH showed a lower value than the averages. The pH, OM and Exch. Ca had slightly increased from 6.3 to 6.6, from 34 to 37 g kg<SUP>-1</SUP>, and from 7.7 in 2000 to 10.6 cmolc kg<SUP>-1</SUP> in 2012, respectively. The order of sample ratios exceeding the optimal range were Avail. P2O5 (83%) > Exch. Ca (80%) > Exch. K (70%) > Exch. Mg (65%) > EC (55%) > OM (48%) > pH (29%) in 2012. The order of sample ratios below the optimal range was OM (25%) > Exch. K (25%) > pH (20%), Exch. Mg and Avail. P2O5 (9%) > Exch. Ca (6%) in 2012. The excessive proportion of pH, Exch. Ca, Exch. Mg and OM slightly increased, while the insufficient proportion of those decreased. Approximately 55% of polytunnel soils exceeding EC 2 dS m<SUP>-1</SUP> was evaluated with salt accumulated soils having the risk of growth disorder of crops. Nutrient contents in polytunnel soils in Korea showed high level especially Avail. P₂O? and Exch. cations. Therefore, recommended fertilization based on soil testing or plant testing is needed for soil nutrient management.

Mouse Single Oral Dose Toxicity Study of DHU001, a Polyherbal Formula

Roh, Seong-Soo,Ku, Sae-Kwang Korean Society of ToxicologyKorea Environmental Mu 2010 Toxicological Research Vol.27 No.1

This study was conducted to obtain acute information of the oral dose toxicity of DHU001, a polyherbal formula in male and female mice. In order to calculated 50% lethal dose ($LD_{50}$) and approximate lethal dose (LD), test material was once orally administered to male and female ICR mice at dose levels of 2000, 1000, 500, 250 and 0 (vehicle control) ml/kg (body weight). The mortality and changes on body weight, clinical signs, gross observation, organ weight and histopathology of principle organs were monitored 14 days after treatment with DHU001. We could not find any mortalities, DHU001 treatment-related clinical signs, changes on the body and organ weights, gross and histopathological findings. The results obtained in this study suggest that $LD_{50}$ and approximate LD in mice after single oral dose of DHU001 were considered over 2000 mg/kg in both female and male mice.

Micronucleus Test of DHU001, a Polyherbal Formula, in Bone Marrow Cells of Male ICR Mice

Seong-Soo Roh,Hyeung-Sik Lee,Sae-Kwang Ku 한국독성학회 2009 Toxicological Research Vol.25 No.4

The genotoxic effects of DHU001, a polyherbal formula were evaluated using the mouse micronucleus test. DHU001 was administered once a day for 2 continuous days by oral gavage to male ICR mice at doses of 2000, 1000 and 500 ㎎/㎏. Cyclophosphamide was used as a known genotoxic agent in a positive control. The appearance of a micronucleus is used as an index for genotoxic potential. In addition, the changes on the total white blood cells and differential counts on the lymphocytes, neutrophils, eosinophils, basophils and monocytes in the prepared blood smears were also conducted to observe the possible immunosuppression. The results indicats that DHU001 showed no genotoxicity effects up to 2000 mg/kg dosing levels and did not influenced on the total white blood cells and differential counts. In addition, it is also considered that there were no problems from cytotoxicity of DHU001 tested in this study because the polychromatic erythrocyte ratio was detected as > 0.41 in all tested groups.

Micronucleus Test of DHU001, a Polyherbal Formula, in Bone Marrow Cells of Male ICR Mice

Roh, Seong-Soo,Lee, Hyeung-Sik,Ku, Sae-Kwang Korean Society of ToxicologyKorea Environmental Mu 2009 Toxicological Research Vol.26 No.4

The genotoxic effects of DHU001, a polyherbal formula were evaluated using the mouse micronucleus test. DHU001 was administered once a day for 2 continuous days by oral gavage to male ICR mice at doses of 2000, 1000 and 500 mg/kg. Cyclophosphamide was used as a known genotoxic agent in a positive control. The appearance of a micronucleus is used as an index for genotoxic potential. In addition, the changes on the total white blood cells and differential counts on the lymphocytes, neutrophils, eosinophils, basophils and monocytes in the prepared blood smears were also conducted to observe the possible immunosuppression. The results indicats that DHU001 showed no genotoxicity effects up to 2000 mg/kg dosing levels and did not influenced on the total white blood cells and differential counts. In addition, it is also considered that there were no problems from cytotoxicity of DHU001 tested in this study because the polychromatic erythrocyte ratio was detected as > 0.41 in all tested groups.