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( Seok Jeong Lee ),( Kyoung Ae Kong ),( Yon Ju Ryu ),( Jin Hwa Lee ),( Jung Hyun Chang ) 대한결핵 및 호흡기학회 2014 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.118 No.-
Background: Osteoporosis is an important comorbidity in patients with COPD. Osteoporosis and its complication such as fracture cause poor quality of life and even mortality. We investigated risk factors of osteoporosis in COPD patients. Methods: We analyzed data from the 2008-2011 KNHANES. Subjects who aged 40 years or more and performed acceptable and qualified spirometry, dual-energy X-ray absorptiometry, the nutrition examination survey, and serum vitamin D measurements were enrolled. COPD was defined as an FEV₁/FVC < 0.7. Osteoporosis was defined as a bone mineral density (BMD) T-score =-2.5 at femur or lumbar spine. Results: A total of 881 subjects with COPD were enrolled, and 141 (16%) had osteoporosis. Epidemiologic and nutritional variables and serum vitamin D levels of osteoporosis group were compared with those of non-osteoporosis group, and variables for multiple logistic regression model were selected. Old age (60-69 years vs. 40-49 years, OR 33.78, 95% CI 3.60-317.14), female (OR 15.54, 95% CI 6.71-36.00), low body mass index (BMI) (< 18.5 kg/m² vs. 18.5-25 kg/m², OR 14.24, 95% CI 4.38-46.31), lack of moderate exercise (exercise vs. lack of exercise, OR 0.41, 95% CI 0.19-0.88), and lower intake of carotene (per 1000 mg increase, OR 0.88, 95% CI 0.79-0.97) were independently associated with osteoporosis in COPD subjects. Although vitamin D deficiency, defined as serum levels < 20 ng/mL, was more prevalent in COPD subjects with osteoporosis, multiple logistic analysis failed to show statistical significance. Conclusions: Old age, female, low BMI, lack of exercise, and lower carotene intake were risk factors of osteoporosis in COPD. Acknowledgement: This was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (2010-0027945).
( Seok Jeong Lee ),( Kyoung Ae Kong ),( Yon Ju Ryu ),( Jin Hwa Lee ),( Jung Hyun Chang ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
Background: Osteoporosis is an important comorbidity in patients with COPD. Osteoporosis and its complication such as fracture cause poor quality of life and even mortality. We investigated risk factors of osteoporosis in COPD patients. Methods: We analyzed data from the 2008-2011 KNHANES. Subjects who aged 40 years or more and performed acceptable and qualified spirometry, dual-energy X-ray absorptiometry, the nutrition examination survey, and serum vitamin D measurements were enrolled. COPD was defined as an FEV₁/FVC < 0.7. Osteoporosis was defined as a bone mineral density (BMD) T-score =-2.5 at femur or lumbar spine. Results: A total of 881 subjects with COPD were enrolled, and 141 (16%) had osteoporosis. Epidemiologic and nutritional variables and serum vitamin D levels of osteoporosis group were compared with those of non-osteoporosis group, and variables for multiple logistic regression model were selected. Old age (60-69 years vs. 40- 49 years, OR 33.78, 95% CI 3.60-317.14), female (OR 15.54, 95% CI 6.71-36.00), low body mass index (BMI) (< 18.5 kg/m² vs. 18.5-25 kg/m², OR 14.24, 95% CI 4.38-46.31), lack of moderate exercise (exercise vs. lack of exercise, OR 0.41, 95% CI 0.19-0.88), and lower intake of carotene (per 1000 mg increase, OR 0.88, 95% CI 0.79-0.97) were independently associated with osteoporosis in COPD subjects. Although vitamin D deficiency, defined as serum levels < 20 ng/mL, was more prevalent in COPD subjects with osteoporosis, multiple logistic analysis failed to show statistical significance. Conclusions: Old age, female, low BMI, lack of exercise, and lower carotene intake were risk factors of osteoporosis in COPD. Acknowledgement: This was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (2010-0027945).
Kong, Byung Ho,Shin, Hyun-Do,Kim, Se-Hoon,Mok, Hyun-Su,Shim, Jin-Kyoung,Lee, Ji-Hyun,Shin, Hye-Jin,Huh, Yong-Min,Kim, Eui-Hyun,Park, Eun-Kyung,Chang, Jong Hee,Kim, Dong-Seok,Hong, Yong-Kil,Kim, Sun Ho Lychnia 2013 International journal of oncology Vol.42 No.5
<P>The presence of glioma stromal mesenchymal stem?like cells (GS-MSLCs) in tumors from glioma patients has been previously reported. The mechanisms through which these cells function as a part of the glioma microenvironment, however, remain incompletely understood. We investigated the biological effects of GS-MSLCs on glioma cancer stem cells (gCSCs), testing the hypothesis that GS-MSLCs alter the biological characteristics of gCSCs. GS-MSLCs and gCSCs were isolated from different glioblastoma (GBM) specimens obtained from patients. In in vitro experiments, gCSCs were cultured alone or co-cultured with GS-MSLCs, and gCSCs cell counts were compared between the two groups. In addition, two groups of orthotopic GBM xenografts in mice were created, one using gCSCs from the monoculture group and one using gCSCs isolated from the co-culture group, and tumor volume and survival were analyzed. Furthermore, in vivo proliferation, apoptosis and vessel formation were examined using immunohistochemical analyses. In vitro cell counts for gCSCs co-cultured with GS-MSLCs increased 3-fold compared to gCSCs cultured alone. In orthotopic xenograft experiments, mice injected with gCSCs isolated from the co-culture group had significantly larger tumor volume, measured on day 40 after injection, and their survival times were shorter. Immunohistochemical analysis showed increased tumor expression of CD31, indicative of enhanced microvessel formation in mice injected with gCSCs co-cultured with GS-MSLCs compared to mice injected with gCSCs cultured alone. However, proliferation (PCNA) and apoptosis (TUNEL) markers showed no significant difference between the two groups. In conclusion, GS-MSLCs may influence the biological properties of gCSCs, shifting them towards a more aggressive status; moreover, increased angiogenesis may be a critical component of this mechanism.</P>
CMOS Transistors with a 70-nm Gate Length for 0.13-μm-Node High-Performance Applications
Kyoung-Seok Rha,Dong-Hun Lee,Eun-Seung Jung,Hae-Kyung Kong,Hyae-Ryoung Lee,Jeong-Ho Lyu,Jeong-Hwan Yang,Jin-Suk Jung,Jung-A Choi,Kwang-Pyuk Suh,Young-Wug Kim 한국물리학회 2004 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.44 No.1
Conventional CMOS transistors for high performance with a 70-nm physical gate length were fabricated to evaluate the 130-nm-technology node. In this work, we enhanced the performance of transistors by using high-dose P+ implantation, a plasma-nitridation gas oxide, a hydrogen prebake (HPB), and a mechanical stress layer. We also reduced the overlap capacitances by using an oset spacer. The NMOS and PMOS have drive currents equal to 880 A/m and 420 A/m, respectively, with Ioff = 10 nA/m at Vdd = 1.2 V. The unit delay of the ring oscillator (1 fan-out) was 11.5 ps.
Prognostic Value of Glioma Cancer Stem Cell Isolation in Survival of Primary Glioblastoma Patients
Kong, Byung Ho,Moon, Ju Hyung,Huh, Yong-Min,Shim, Jin-Kyoung,Lee, Ji-Hyun,Kim, Eui-Hyun,Chang, Jong Hee,Kim, Dong-Seok,Hong, Yong-Kil,Kim, Sun Ho,Lee, Su-Jae,Kang, Seok-Gu Hindawi Publishing Corporation 2014 Stem cells international Vol.2014 No.-
<P>Cancer stem cells (CSCs) have been reported to be critical in the initiation, maintenance, and progression of cancers. The expression of stem cell markers, such as podoplanin (PDPN), CD133, and nestin, may have been correlated with malignant progression. However, the effects of CSCs and stem cell markers on clinical outcomes in cancer patients remain unclear. In this study, we assessed the prognostic roles of glioma CSCs (gCSCs) isolation and stem cell markers in patients with primary glioblastoma (pGBM). A cohort of 39 patients with pGBM was separated into two groups, those positive or negative for gCSCs, and the correlation between gCSC and patient survival was evaluated. We observed significantly different cumulative survival (<I>P</I> = 0.045) when comparing patients positive for gCSCs patients and negative for gCSC. Among the patients positive for gCSCs, we observed no significant differences in survival between those whose gCSCs were each positive or negative for PDPN, CD133, or nestin. This study strongly supports the prognostic value of gCSCs isolation on the survival of patients with pGBM.</P>
VHS (viral hemorrhagic septicemia)의 원인병원체인 VHSV (genotype IVa)에 대한 단클론 항체 개발
공경희 ( Kyoung-hui Kong ),오명주 ( Myung-joo Oh ),장민석 ( Min-seok Jang ),김춘섭 ( Choon-sup Kim ),김위식 ( Wi-sik Kim ) 한국어병학회 2019 한국어병학회지 Vol.32 No.2
본 연구에서는 넙치(Paralichthys olivaceus)로부터 분리한 바이러스성출혈성패혈증바이러스(viral hemorrhagic septicemia virus, VHSV, genotype IVa)에 대한 단클론 항체(monoclonal antibody, MAb)를 개발하였다. VHSV에 대한 항체를 생산하는 총 5개의 hybridoma clone을 생산하였다. 4개의 MAbs(2C10, 18H4, 23H6, 30B7)는 glycoprotein을 인식하였고, MAb 15E10은 nucleocapsid protein을 인식하였다. 5개의 MAbs는 western blot 상에서 VHSV에 감염된 세포와 넙치시료에 반응하였으나, 정상 세포와 넙치시료에는 반응하지 않았다. 또한 ELISA상에서 VHSV에만 반응하였고 6종의 어류바이러스(IHNV, HIRRV, SVCV, IPNV, MABV, NNV)에는 반응하지 않았다. 이상의 결과, 본 연구에서 제작된 MAbs는 VHSV에만 특이적으로 반응하는 것이 확인되어 VHSV를 검사하는데 사용될 수 있을것으로 사료된다. We developed and subsequently characterized mouse antibodies (MAbs) against viral hemorrhagic septicemia virus (VHSV, genotype IVa), the causative agent of VHS. Five hybridoma clones secreting MAbs against VHSV were established. The MAbs recognized the glycoprotein (MAbs 2C10, 18H4, 23H6, and 30B7) and nucleocapsid protein (15E10) of VHSV by western blot analysis. All five MAbs reacted with VHSV-infected cells and tissue homogenates of VHSV-infected olive flounder (Paralichthys olivaceus) by western blot analysis. Whereas, no reactivity was observed in normal cells and tissue homogenates of normal olive flounder. Moreover, these MAbs reacted with VHSV, but did not react with other fish viruses (infectious hematopoietic necrosis virus, hirame rhabdovirus, spring viraemia of carp virus, infectious pancreatic necrosis virus, marine birnavirus, and nervous necrosis virus) by enzyme linked immunosorbent assay (ELISA). These results indicate that the MAbs are specific to VHSV and can be of value in VHSV detection.
Chromothripsis in Treatment Resistance in Multiple Myeloma
Lee, Kyoung Joo,Lee, Ki Hong,Yoon, Kyong-Ah,Sohn, Ji Yeon,Lee, Eunyoung,Lee, Hyewon,Eom, Hyeon-Seok,Kong, Sun-Young Korea Genome Organization 2017 Genomics & informatics Vol.15 No.3
Multiple myeloma (MM) is a malignant disease caused by an abnormal proliferation of plasma cells, of which the prognostic factors include chromosomal abnormality, ${\beta}$-2 microglobulin, and albumin. Recently, the term chromothripsis has emerged, which is the massive but highly localized chromosomal rearrangement in response to a one-step catastrophic event. Many studies have shown an association of chromothripsis with the prognosis in several cancers; however, few studies have investigated it in MM. Here, we studied the association between chromothripsis-like patterns and treatment resistance or prognosis. First, we analyzed nine MM cell lines (U266, MM.1S, RPMI8226, KMS-11, KMS-12-BM, KMS-12-PE, KMS-28-BM, KMS-28-PE, and NCI-H929) and bone marrow samples of four patients who were diagnosed with MM by next-generation sequencing-based copy number variation analysis. The frequency of the chromothripsis-like pattern was observed in seven cell lines. We analyzed the treatment-induced chromothripsis-like patterns in KMS-12-BM and KMS-12-PE cells. As a result, breakpoints and chromothripsis-like patterns were increased after drug treatment in the relatively resistant KMS-12-BM. We further analyzed the patients' results according to the therapeutic response, which was divided into sensitive and resistant, as suggested by the International Myeloma Working Group. The chromothripsis-like pattern was more frequently observed in the resistant group. In the sensitive group, the frequency of the chromothripsis-like pattern decreased after treatment, whereas the resistant group showed increased chromothripsis-like patterns after the treatment. These results suggest that the chromothripsis-like pattern is associated with treatment response in MM.