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양식산 터봇, Scophthalmus maximus에서의 Irido-like virus 감염
김위식,김기홍,김춘섭,김영진,정성주,정태성,신이치, 키타무라,마모루, 요시미즈,오명주 한국어병학회 2003 한국어병학회지 Vol.16 No.3
본 연구는 병리조직학적 및 전자현미경적 방법을 통해 양식산 turbot에서 발생하는 irido-like virus의 감염증을 소개하고자 한 것이다. 2003년 6월 전북 고창 소재 육상수조에서 양식중인 turbot치어에서 50∼70%의 대량폐사가 발생하였다. 병어는 사료섭취가 저하되고 힘없이 유영하다 폐사되었으며, 죽기 직전의 어류는 복부가 팽만되고 안구가 돌출되며 체색이 옅어지는 증상을 보였다. 해부학적 조사 결과, 아가미 빈혈과 간이 퇴색되어 있었으며, 특히 비장조직이 심하게 비대되어 있었다. 병어에 대한 조직학적 검사에서 비대 세포를 특징으로 하는 특이 병변이 주로 신장과 비장의 조혈조직에서 관찰되었으며, 아가미, 심장, 위, 장, 간, 췌장, 지방 조직에서도 관찰되었다. 그리고 전자현미경적 관찰 결과, 비대 세포의 세포질내에서 육각형의 지름이 136∼159 ㎚인 Irido-like virus를 관찰할 수 있었다. The high mortality of cultured juvenile turbot, Scophthalmus maximus occurred in Gochang on June, 2003. The diseased fish was lethargic with reduced feed intake. Grossly, these fish showed pale body, abdominal extension and exophthalmia. The dominant internal gross features of diseased fish were severely enlarged spleen, pale gills and or liver. Diseased fish histologically showed basophilic enlarged cells in the kidney, spleen, gill, heart, stomach, intestine, liver, pancreas and adipose tissue. Transmission electron microscopy (TEM) reveled hexagonal virions in the cytoplasm of necrotic cells. The viral particles lead a central electron-dense core and an electron translucent zone, and were 136-159 nm in diameter. These results suggest that the virus belonging to the iridoviridae was responsible for the mortality of cultured juvenile turbot.
국내의 양식산 무지개송어 성어로부터 분리된 IHNV의 G protein gene에 대한 연구
김기홍,김위식,김춘섭,김영진,정태성,신이치, 키타무라,정성주,오명주 한국어병학회 2003 한국어병학회지 Vol.16 No.3
An Infectious hematopoietic necrosis virus strain (IHNV-RTK) was isolated from cultured rainbow trout at Kumi in Korea during 2000 and 2001. In the RT-PCR amplification with the specific primer set designed from IHNV G protein region, a 540 bp PCR product was amplified from the RTK strain. The RTK strain showed higher sequence homology with the published IHNV G protein genes (RB-76, LR-73, Col-85, and Carson-89).
자연산 숭어(Mugil cephalus)의 Myxobolus sp. 감염증
김위식,이무근,박경희,정성주,오명주 한국어병학회 2003 한국어병학회지 Vol.16 No.1
2002년 2월 광양만에서 자연 서식하는 숭어에서 폐사가 발생하였다. 그 폐사 원인을 조사하는 과정에서 Myxobolus sp.의 감염을 확인하여, 본 연구에서는 병리조직학적 검사를 통한 Myxobolus sp.의 감염증을 보고하게 되었다. Myxobolus sp.의 cyst는 숭어의 장간막, 간, 아가미 및 인두낭에서 관찰되었으며, 병리조직학적 검사에서 Myxobolus sp.의 전신감염을 확인할 수 있었다. 감염 충체는 길이 10-12 ㎛, 폭 9-10 ㎛, 두께 6.4-7.2 ㎛의 크기로서 체내에 길이 4-5.2 ㎛, 폭 2.5-3.3 ㎛의 2개의 극낭을 가지고 있었다. The mortality of wild mullet, Mugil cephalus was detected in Kwang-Yang bay on February, 2002. The mullet were infected with Myxobolus sp., the cysts of Myxobolus sp. were found in the mesentery, liver, gill and pharyngeal pocket. The histological findings suggested a systemic infection by the Myxobolus sp.. The spores were measured 10-12 (10.9)㎛ in length, 9-10 (9.4) ㎛ in width, 6.4-7.2 (6.8) ㎛ in thickness, with polar capsules of 4-5.2 (4.4) ㎛ in length and 2.5-3.3 (2.9) ㎛ in width.
Kim, Jae-Ok,Kim, Soo-Jin,Kim, Jong-Oh,Kim, Wi-Sik,Oh, Myung-Joo Elsevier 2018 Aquaculture Vol.489 No.-
<P>Nervous necrosis virus (NNV) is one of the most serious fish pathogens which causes damage in > 120 species of cultured marine fishes worldwide including sevenband grouper (Hyporthodus septemfasciatus). The present study was undertaken to compare NNV genomic segments RNA1 and RNA2 through qRT-PCR and determine viral infectivity in eight organs of dead or survived sevenband grouper after intramuscular (IM) injection or immersion challenge by NNV. IM injection with NNV at 10(0.5), 10(1.5), 10(2.5) and 10(3.5) TCID50/100 mu L/fish resulted in cumulative mortality of 0%, 20%, 40% and 100%, respectively. Immersion challenge with NNV at 10(1.5), 10(3.5) and 10(5.5) TCID50/mL resulted in cumulative mortality of 0%, 40% and 60%, respectively. The minimum NNV concentration pathogenic to fish by IM injection was approximately at least 10-fold lower compared to that by immersion challenge route. The ratio of the RNA1 segment and infective particles in dead fish was higher than RNA2/TCID50, which indicates that RNA1 was overproduced than RNA2 segment. This implies that RNA2 segment is very efficient in packaging infectious particles. Brain and eye were found to have the highest NNV titer and copy number. The gill, spleen and kidney were detected higher than the other non-nervous tissues, suggesting that these tissues can be used for NNV diagnostics although they do not seem to represent the infective tissues. In survived fish, NNV was under the detection limit although NNV copy number was detected, which indicates that although NNV could get into the body, it failed to produce complete virion.</P>
Kim, Wi-Sik,Oh, Myung-Joo Inter-Research 2014 Diseases of aquatic organisms Vol.109 No.1
<P>Aquabirnavirus is an epizootic virus in Japanese eel Anguilla japonica farms in Korea, although its origin is unclear. In the present study, nucleotide sequences of the VP2/NS junction region of 9 Korean aquabirnaviruses from cultured eel in various areas of Korea during 2000-2009 were analyzed to evaluate their genetic relatedness to worldwide isolates. The nucleotide sequences showed more than 94.2% identity among the 9 Korean eel isolates, 71.2% identity among 16 Korean isolates from freshwater and marine fish, and 71.1% identity among 25 worldwide isolates. All 9 isolates in this study were phylogenetically classified into genogroup II, including isolates from Denmark, Spain, Taiwan and Japan, and were discrete from salmonid and marine fish isolates (genogroup I and VII) in Korea. These results suggest that the Korean eel isolates have most likely been introduced from outside the country and not from coastal areas of Korea.</P>