食生活의 노래, 製作 硏究 : Five Basal Food Groups

朴圓記,金慶洋 조선대학교 교육연구소 1987 교과교육연구 Vol.10 No.-

흰쥐에서 절식과 고지방식이가 콜레스테롤 및 지방대사에 미치는 영향

양정례,박경아,진현화,김양하 창원대학교 생활과학연구소 2001 생활과학연구 Vol.5 No.-

This study was performed to investigate the effects of diet pattern on lipid and cholesterol metabolism in rats. Sprague-Dawley rats were fed four types of diets for four weeks, respectively: a control diet, 40% energy restriction diet, high animal fat diet or high plant fat diet. Control and 40% energy restriction diets were composed by AIN-76 diet and high fat diets were composed by 53.6% of animal or plant fat. In rats fed with 40% energy restriction diet showed significantly low body weight gain, liver, abdominal and subcutaneous adipose tissue weights compared to rats fed with control diet. In rats fed with high animal fat diet showed significantly high body weight gain, liver, abdominal and subcutaneous adipose tissue weights compared to rats fed with control diet. In rats fed with high plant fat diet showed similar body weight gain. The increase of adipose tissue weight was much smaller in rats fed with high plant compared to rats fed with high animal fat diet implying the effect of high fat diet on fat accumulation on adipose tissue may defend on the kinds of fats. Plasma total cholesterol and triglyceride concentrations were significantly decreased in rats fed high plant fat diet. However, liver total cholesterol and triglyceride concentrations were signigficantly increased in rats fed high animal fat diet. The fibrinolytic activity of serum was significantly increased in rats fed high plant fat diet compared to rats fed with control diet.

Carbonic Anhydrase Inhibitors가 Lipopolysaccharide에 의해 유도된 골흡수에 미치는 영향

朴亮鎬,車敬石,金世源 단국대학교 치의학연구소 1992 논문집 Vol.4 No.-

To study bone resorption mechanism, effect of LPS on the ^45Ca release from fetal rat ulnae and radii, and effects of carbonic anhydrase inhibitors on the LPS-induced bone resorption in organ culture were studied. Ulnae and radii were removed from 19 day old fatal rats, prelabelled by subcutaneous injection of 200μCi ^45CaCl_2 into their mother on the 17th day of gestation. Radioactivities of ^45Ca released into media were determined after 24, 48 and 72 hours. Effects of LPS and carbonic anhydrase inhibitors were observed by the ratio of % release of ^45Ca between paired control and experimental group. The observed results were as follows : 1. LPS(1㎍/ml) supplemented in media for 72hours increased the ^45Ca release significantly after 48 and 72 hours of culture and LPS(10㎍/ml) increased the ^45Ca release significantly after 72 hours of culture. 2. LPS-induced ^45Ca release was not inhibited significantly by 1mM sulfanilamide but inhibited significantly by 10mM sulfanilamide after 48 and 72 hours of culture. 3. LPS-induced ^45Ca release was not inhibited significantly by 0.1mM dichlorphenamide but inhibited significantly by 1mM dichlorphenamide after 48 and 72 hours of culture. 4. LPS-induced ^45Ca release was not inhibited significantly by 1mM acetazolamide but inhibited significantly by 5mM acetazolamide after 72 hours of culture.

SEM-EDX를 이용한 대기 점오염에서 배출된 개별입자상 오염물질의 물리화학적 특성

박정호,양수명,강경희,정재우 진주산업대학교 2004 論文集 Vol.43 No.-

The purpose of the study was to classify individual particle emitted from the point source of air pollution emissions in Kyongsangnam-do by SEM/EDX (scanning electron microscopy / energy dispersive X-ray analyzer). The SEM/EDX provided various physical parameters including particle`s optical diameter and chemical information. The total of 344 individual particle sample were collected at 5 point source including coal-fired power plant, incinerator, and oil boiler. Thus number fraction of each particle can be estimated based on chemical composition. To obtain number fraction of each particle class, an agglomerative hierarchical cluster analysis was initially applied to create particle classes for each sample. The study provides opportunities to identify particles source quantitatively and to develope various receptor models.

뇌손상시 교세포의 작용에 관한 전자현미경적 연구

양남길,고정식,안의태,박경호 순천향대학교 1984 논문집 Vol.7 No.1

뇌조직의 별성 또는 질병과정에서 각종 신경교세포의 역활을 비교 검토하기 위하여 일차적으로 창상 치유과정의 실험을 시행했다. 뇌조직의 미세구조가 확연하지 못한 부위가 많은 점을 고려하여 이번 실험에서는 이미 저자들이 오랫동안 연구하여 미세구조의 특징을 잘 알고 있는 뇌기저핵의 fundus striati를 주변구조로부터 외각적으로 분리시킨 상태에서 1, 2, 3, 7, 14, 28일간 생존시켜 창상 치유과정에서, 각 미세구조별로 퇴행과정과 교세포 종류별 활동상황을 전자현미경으로 관찰하여 다음과 같은 결론을 얻었다. (1) 창상 후 형태학적 변화는 투사세포의 괴사, 외래 신경축삭과 종말의 괴사, 괴사종말과 연접한 연접후구조의 부종과 극돌기 합입등으로서 이미 발표한 성적들??과 같았다. (2) 수술초기에는 원형질성 섬유세포가 neuropil전역에 걸쳐 활동성으로 바뀌고, 모든 퇴행성 구조를 감싸면서 다른 구조와 분리시켰다. (3) 분리된 퇴행성 구조물은 용해되기 시작했다. (4) 미세교세포가 증가하였고 이들 세포는 다량의 지방소적을 함유하고 있었다. (5) 혈관주위세포는 기저막으로 싸인채 팽대되며, 주변에는 미세교세포와 이행형이면서 기저막이 없는 세포들이 많이 출현하여 뇌대식세포의 혈액세포 또는 혈관주위세포 기원설에 일치하는 형태학적 소견을 보였다. (6) 회돌기교세포는 변성축삭으로부터 수초의 회수작업등의 수동적 반응을 보였다. (7) 교세포의 비교판독 결과 대식세포는 외인성 이물질을 파괴소화시키는 작업을 수행하며, 원형질성 성상세포가 모든 변성구조물의 용해·흡수·제거작용을 이행하고, 최종적으로 섬유성 성상교세포가 빈자리의 섬유화를 완성한다. (8) 조직의 섬유화가 치유과정과 동시에 이루어지는 것은 뇌의 신경세포재생이 이루어지지 못하는 주요 원인으로 생각된다. Structural variations of the neuroglial cells in the degenerating nervous tissues are points of arguements in the neuromorphological field. The short of ultrastructural information on the specific nuclei is one of the critical points in the study of neuroglial activities, because the exact readings of the ultrastructural elements are essential for the differentiation of glial-target relationships. Having the experiences of extensive studies on the fundus striati nucleus (Nucleus accumbens septi) during the last 10 years, we have studied the glial-target relationships following the surgical isolation of the fundus striati uncleus of cat. Surgery was performed by vertically inserting a stainless stell tube-knife, measuring 5mm in diameter, into the brain down to the base of the skull. The blood supply to the tissue cylinder was maintained through the vessels at the base of the skull. The blood supply to the tissue cylinder was maintained through the vessels at the base of the brain. After the survival time of 1, 2, 3, 7, 14 and 28 days, cats were perfused with a mixture of 1% paraformaldehyde-1% glutaraldehyde in phosphate buffer (pH 7.4) and postfixed in osmium tetroxide solution. The ultrathin sections were counterstained with uranyl acetate-lead citrate, and were examined in a JEM 100CX electron microscope. The results are as follow: 1. In the primary stage, the protoplasmic astroglial cells are activated throughout the neuropil. They fill the large area between neuronal elements, and separate the degenerating elements from the intact elements. 2. The astroglial cells attack and dissolve the degenerated elements. 3. The microglial cells increase in number and are activated. 4. In the later stage, around the capillaries, there accumulate many hypertropied pericytes, activated brain macrophages, and the cells of transitional forms between pericytes and macrophages. 5. Ameboid microglia or macrophages also can separate the altering elements from the intact elements. 6. Oligodendroglia shows passive reaction of retraction its myelinating lamellae from degenerating axons. 7. Glial fibrosis actively spreading in the degenerating nervous tissue was suspected as the possible reason of loss of regenerative function in central neuronal cells.

咸平灣에 對한 生態學的 硏究

朴炅洋,宋泰坤 木浦大學校沿岸生物硏究所 1987 沿岸生物硏究 Vol.4 No.1

1984년 2월부터 1985년 9월 사이에 함평만의 생태학적 기초연구를 위하여 수질, 저질 및 규조류에 대한 조사를 실시하였다. 해수에 있어서 수온의 분포는 최저 -0.2℃에서부터 최고 27.6℃의 큰 변화를 보였으며 염분량은 29.32∼32.51‰, 용존산소량 3.66∼8.08㎎/ℓ, COD 0.56∼2.67㎎/ℓ, 총질소량 0.77∼8,36㎍-at/ℓ, 그리고 인산염 0.28∼0.89㎍-at/ℓ범위였으며, 이들 환경요인의 수직적인 분포양상은 표층과 저층간에 큰 변화가 없는 혼합구조를 보였다. 부유물질량은 4.0∼40.0㎎/ℓ 범위였는데 김의 채취시기에 그 양이 많았다. 저질은 만의 입구부와 간석지의 일부를 제외하고는 silt와 clay로 구성되었으며, 이들의 pH는 6.6∼7.7, 전기전도도는 1.6∼5.0mmhos/㎝ 범위였다. 저질 중에 함유된 유효인량은 1.15∼6.17ppm, 전질소량 0.20∼0.74㎎/g, 페오파이친 색소량 0.1∼5.6㎍/g, COD 6.2∼12.7㎎/g 및 유화물량 0.03∼0.70㎎/g였다. 이들의 수평적인 분포는 서로 유사하엿으며, COD,는 부영양화의 상한치에 많이 미달되는데 비해 유화물량은 상한치를 넘는 곳도 있었다. 조사기간동안 채집된 규조류는 22속 41종 1변종 1품종으로 총 44종류였으며, Coscinodiscus속의 출현율이 높았고, 4월과 7월에는 각각 Paralia sulcata와 Eucampia zoodiacus가 많이 나타났다. 규조류의 현존량은 162×10³∼8,910×10³cell/㎥로 11월에 그 양이 많았고 6월에 적었다. Physicochemical properties of water and bottom mud, and the species composi tion and biomass of diatoms were studied in Hampyung Bay for the period of Feburary, 1984 and September, 1985. The variation of water temperature was great, ranging from - 0.2 to 27.6℃. Water qualities such as salinity, DO, COD, total nitrogen and phosphorous showed no significant difference in relation to water depth. Suspended substance content of water varied from 4.0 to 40.0㎎/ℓ and the highest value showed in late winter and early spring. Soil particels of bottom mud was composed of clay and silt in almost sites except the mouth of Hampyung Bay. Horizontal distribution in quantities of physicochemical factors of bottom mud such as pH, conductivity, available phosphorus, phaeophytin content, COD and sulfide was almost same pattern. The diatoms collected during the investigated period were composed of 41 species, 1 variety, 1 forma in 22 genera. Genus Coscinodiscus was a high frequent group. Paralia sulcata was dominant species in April and Eucampica zoodiacus in July. Cell number of diatoms fluctuated with a range from 162 10³cells/㎥ to 8,910 10³cells/㎥.

중학교 가정 교과 수행평가 방법 및 도구 개발

박선영,조필교,이양심,윤인경,임양순,최창숙,장명희 한국 가정과 교육 학회 1998 한국가정과교육학회지 Vol.10 No.2

The purpose of the study is to develop a Performance Assessment Method & Tools for Home Economics at middle school. Through the content analyses of middle school Home Economics curriculum and 8 textbooks of lst, 2nd, and 3rd grade respectively, teching elements for each content area were drawn and each specific learning objectives were idntified accordingly. In addition, assessment element for student's performance in each teaching element were specified for analysis. Analytic framework for the development of Performance Assessment Method and Tools were proposed by each teaching element. Beased on the analytic framework proposed, a model method and tools were developed by 10 different test measures of description written test, statement written test, oral test, pro and con discussion, performance test, experiment, interview, observation, self-test report, and portfolio. The model proposed in the study has been modified and improved through the feasibility studies of experts' examination and field research application. The model assessment method and tools for the study has been compleeted finally by proposing 45 performance assessment tools for the content areas of food, clothing, housing, resource management and consumerism, human development and family relationship.

우리나라 소아에서의 β- Lactamase 산생 Escherichia의 장관내 존재

박현수,김경희,조양자,서인수 대한감염학회 1989 감염 Vol.21 No.2

In order to evaluate the possibility that facal E. coli might serve as a reservoir of β-lactamases for Haemophilus influenzae, Neisseria gonorrhoeae, or other members of the Family Enterbacteriaceae, 135 fecal E. coli isolates from Korean children uder two years of age were tested for resistance to ampicillin (??) and for β-lactamases activity. ?? was found in 115(85%) strains of E. coli tested. Of the 115 ?? E. coli, 87(76%) were β-lactamases producers. High rates of ?? and β-lactamases activity were found uniformly in various pathogenic types of E. coli. Our observations raise questions concerning the importance of fecal E. coli in the prevalance of β-lactamase producing bacteria in the community. Fecal E. coli of Korean children may be important in the epidemiology of β-lactamases producing pathogens in humans.

사람태아 간적혈구형성에 따른 간세포의 변화

박영양,김경용,이원복,김동창 중앙대학교 의과대학 의과학연구소 1992 中央醫大誌 Vol.17 No.1

The ultrastructural development of fetal liver cells according to the activity of hepatic erythropoiesis was studied with 15 human fetal livers from 7 to 34 weeks of gestation. 1) The 1st state(7-8 weeks of gestation) : The nucleus of hepatocyte was oval with granular euchromatin evenly dispersed throughout the interior and prominent nucleoli. The hepatocytes often exhibited many mitochondria and short dilated rERs but no sERs and glycogen deposit. Two types of reticulum cells with intracellular organells developed with close relation with erythropoietic cells. The nucleus of the 1st type was similar with that of hepatocyte and the cytoplasm was full of well-developed multiayered rERs and numerous mitochondria. The nucleus of the 2nd type was irregular and has high electron-dense nucleoplasm. and the cytoplasm was packed with more developed multiayered rERs and oval mitochondria. 2) The 2nd state(9-22 weeks of gestation) : Most of hepatocytes differentiated well and exhibited numerous mitochondria and free ribosome, 5 to 6 layered rER, glycogen deposit, and there were sppearance of sER and hemosiderin in the cytoplasm. With age, the 2nd type reticulum cell became the majority and had more glycogen deposit and less rER. The space of Disse was well-developed but the sinusoidal wall still immature. 3) The 3rd state(23-34 weeks of gestation) : The majority of hepatocytes were mature cells. Reticulum cells, greatly decreasing with age, exhibited increasing lipid storage and decrease of mitochondria and rER. In summary, the maturation of hepatocyte and activity of reticulum cell may influence the hepatic erythropoiesis.

皮膚自家移植후의 形態學的 變化에 관한 硏究

梁原模,李永浩,申台均,朴景蘭,金源式 충남대학교 의과대학 지역사회의학연구소 1989 충남의대잡지 Vol.16 No.2

The skin of abdominal wall homografted t the back of rats and observed the morphological changes of grafted tissues and mother tissues on the 1 day, 1 week & 2 weeks after operation. For light microscopic observation, the tissues were fixed within 10% neutral buffered formalin solution and stained with routine hematoxylin & eosin and alcian blue-safranin. The results were as follows; 1. The grafted abdominal skin were degenerated markedly on 1 day after operation but some cells near hair follicle remained alive. 2. In the grafted skin, the epidermis was formed newly at the upper part of muscle layer on 1 weeks after operation. 3. The epidermis of grafted skin was thicker than mother skin on 2 weeks after operation, and there were many small cells having long nucleus arranged vertically to the basal lamina. 4. Few mast cells were found in the lower portion of muscle layer on 1 day after graft operation, but the mast cells which contain heparin mainly increased in number gradually from 1 weeks to 2 weeks after operation.