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Kang, Jae Seung,Kim, Ha Na,Jung, Da Jung,Kim, Jee Eun,Mun, Ga Hee,Kim, Yeong Seok,Cho, Daeho,Shin, Dong Hoon,Hwang, Young-Il,Lee, Wang Jae Williams & Wilkins 2007 The Journal of investigative dermatology Vol.127 No.3
It is well known that UVB (290–320 nm) induces inflammation in skin by the transcription and release of cytokines and chemokines from skin keratinocytes. In addition, it is considered that intracellular reactive oxygen species (ROS) plays an important role in UVB-induced inflammatory response in the skin. Therefore, we investigated the effect of vitamin C, a potent antioxidant, on the regulation of UVB-induced skin inflammation via the modulation of chemokines production. Vitamin C uptake into keratinocytes is increased by UVB irradiation in a time- and dose-dependent manner through the translocation of sodium-dependent vitamin C transporter-1 (SVCT-1), a vitamin C-specific transporter, from the cytosol to the membrane. To evaluate the effect of vitamin C on the chemokine mRNA expression, we performed RNase protection assay. As a result, there was a remarkable change in chemokine mRNA expression, especially IL-8 and monocyte chemoattractant protein (MCP)-1 expression. In addition, increased IL-8 and MCP-1 mRNA expressions were suppressed by vitamin C treatment. We also confirmed the results of protein levels measured by ELISA. Taken together, vitamin C uptake is increased in UVB-irradiated keratinocytes through the translocation of SVCT-1 and regulates inflammatory response in the skin via the downregulation of IL-8 and MCP-1 production.Journal of Investigative Dermatology (2007) 127, 698–706. doi:10.1038/sj.jid.5700572; published online 28 September 2006
생쥐의 卵子와 初期胚子에 對한 Clomiphene Citrate의 細胞毒性에 關한 硏究
金惠敬,潘勝一,金洋一,文蓮子,朴承澤,鄭然泰 圓光大學校 醫科學硏究所 1993 圓光醫科學 Vol.9 No.1-2
In order to elucidate the cytotoxicity of clomiphene citrate, the rate of in vitro fertilization(IVF) of ova and the developmental rate of early embroys in A-strain mouse were examined. The rate of ova cleavaging to 2-cell stage by IVF was remarkably decreased(12.6%) at the concentration of 10μg/㎖ of clomiphene than that of the control(65.2%) when the ova fertilized in vitro were cultured for 24 hours in the untreated medium after treatment ova with clomiphene for 5 hrs. But there was no ovum cleavaging to 2-cell stage at 50μg/㎖ clomiphene except only a few 2-polar bodied ova. The developmental rate of mouse early embryos was decreased dose-dependently. Especially at 25μg/㎖ of clomiphene, the number of embryos cleavaging to blastocyst from 2-cell stage(41.4%) was decreased remarkably compared with that of the control(87.2%). And only a few embryos were developed to 8-cell stage at clomiphene concentration of 50μg/㎖ after 2-cell embryos were incubated for 72 hours in clomiphene-treated medium. Morphological changes such as fragmentation and fusion of blastomeres, cytolysis and developmental retardation were increased with dose-dependently. These results suggest that clomiphene has cytotoxic effect by the decrease of the rate of IVF, the developmental rate and the degenerative changes of murine ova and early embryos.
심태용,신성휴,오승일,문정환 한국운동역학회 2004 한국운동역학회지 Vol.14 No.3
T. Y. SHIM, S. H. SHIN, S. I. OH, J. I-. MUN. EMG Analysis for Investigation Muscle-Collaborated Relationship during Golf Swing. Korean Journal of Sport Biomechanics, Vol. 14, No. 3, pp. 177-189, 2004. Kinematic and kinetic analysis using 3D Motion Capture system are common, yet there is little in the literature that discuss the relationship and coactivity between muscles during the golf swing. The purpose of this study was to describe the relationship between the employed 16 muscles during golf swing. We could observe 3 muscle patterns such as 'Line' shape, 'L' shape, and 'Loop' shape for the golf swing activity. The 'Line' shape indicates that two muscles act almost perfectly in phase, and the 'L' shape represents that two muscles act in a reciprocating manner(When one is active, the other is quiescent and vice versa). And the 'Loop' shape indicates that two muscles act sequently(After one is active, the other act). In these results, we knew the muscle patterns during golf swing is similar to the patterns during gait. And we presented it was possible to show the consistence of golf swing through the frequency analysis of muscle patterns. We believe that the results potentially useful for the golf players and coaches to analyze their performance.
Mun, Ga Hee,Kim, Myeung Ju,Lee, Ju Hyun,Kim, Hyun Jun,Chung, Yoon Hee,Chung, Young Bae,Kang, Jae Seung,Hwang, Young Il,Oh, Seung Ha,Kim, Joong-Gon,Hwang, Douk Ho,Shin, Dong Hoon,Lee, Wang Jae Wiley Subscription Services, Inc., A Wiley Company 2006 Journal of neuroscience research Vol.83 No.5
<P>Sodium-dependent vitamin C transporters (SVCTs) is known to transport the reduced form of ascorbic acid into the cell, whereas the oxidized form of vitamin C (VC) is moved through a facilitative sugar transporter, such as glucose transporter (GLUT). With regard to the distribution of SVCT1 and -2 within the various organs, they were reported to be expressed in different types of cells. Especially in the central nervous system, only SVCT2 mRNA was expressed mainly in neurons and some types of neuroglial cells. However, data on the expression of SVCT proteins in the brain are scant. Therefore, we tried to develop comprehensive data on the distribution of SVCT proteins in adult rat brain by using immunohistochemical techniques for the first time. In our study, SVCT2 immunoreactivities (IRs) were intensely localized in the neurons of cerebral cortex, hippocampus, and Purkinje cells of cerebellum, and much weaker SVCT2 IRs were found in the other brain regions. Judging from double-immunohistochemical data, most of the cells expressing SVCT2 IRs were likely to be neurons or microglia, even though the cells in choroids plexus or ependymal cells around the ventricles also exhibited SVCT2 IRs. Complete mapping of the distribution of SVCT2 IRs was available by using a semiquantitative method. The subcellular localization of SVCT proteins is necessary for understanding the exact role of the protein, so the current overall mapping of SVCT IRs in the rat brain could be the basis for further studies on related subjects. © 2006 Wiley-Liss, Inc.</P>
( Jae Il Kim ),( Sang Mun Bae ),( Eun Ju Do ),( Yeon Mi Ryu ),( Sang Yeob Kim ),( Seung Jae Myung ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
Background: Molecular imaging in endoscopy, which uses the molecular signature of cells for targeted imaging of GI pathologies, may be useful for improved detection and treatment of colorectal malignancies. Colon cancer secreted protein-2 (CCSP-2) has been reported as a novel protein expressed in colon adenomas and cancers but not in normal colon tissue. The aim of this study was to determine whether molecular imaging using a CCSP-2-targeted probe can detect colorectal tumors. Methods: Anti-CCSP-2-antibody was labeled with fi uorescent dye. Mouse mAb IgG1 Isotype control was used for control antibody to CCSP-2 antibody. To anticipate apotential use of CCSP-2-targeted imaging in colonoscopy, the topical application of labeled antibody was simulated by incubation of frozen tissue specimens from 6 colorectal cancer patients in fi uorescently labeled antibody solution. We observed the change of fi uorescence signal intensity after incubation. Ex vivo molecular imagings were correlated with histopathology, immunohistochemistry. Results: Molecualr imagings using a CCSP-2-targeted probe showed that all colorectalcancer tissues displayed high signal intensities compared with adjacent normal mucosas. The median tumor-to-background fi uorescence ratio was 3.3 (range 2.3-4.3). In contrast, imagings using a mouse mAb IgG1 control probe showed similar signal intensities of colorectal cancer tissues and adjacent normal mucosas. IHC showed CCSP-2 expressions in all colorectal cancer tissues. There were no CCSP-2 expressions in adjacent normal mucosas. Conclusions: CCSP-2, as a novel protein that distinctly expressed in colon adenomas and cancers, is a promising marker for detection of colorectal tumors. This study demonstrates a rigorous strategy for targeted detection to localize colonic dysplasia that can be distinguished in the large area of colonic mucosa.